WB (Western Blot)
(Detection of human Bub3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Bub3 antibody AAA210909 (lot AAA210909-1) used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human Bub3 Polyclonal Antibody | anti-Bub3 antibody
Rabbit anti-Bub3 Antibody, Affinity Purified
Gene Names
BUB3; BUB3L; hBUB3
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Bub3, Antibody; Rabbit anti-Bub3 Antibody, Affinity Purified; testicular tissue protein Li 27; BUB3 budding uninhibited by benzimidazoles 3 homolog; BUB3L; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog; hBUB3; mitotic checkpoint component; mitotic checkpoint protein BUB3; Mitotic checkpoint protein BUB3; BUB3, mitotic checkpoint protein; anti-Bub3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-Bub3 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Predicted Reactivity
Mouse, Bovine, Orangutan
Immunogen
Between 278 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Bub3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Bub3 antibody AAA210909 (lot AAA210909-1) used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human Bub3 by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Bub3 antibody AAA210909 (lot AAA210909-1) used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human Bub3 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Bub3 (Cat. No. AAA210909) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Bub3 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Bub3 (Cat. No. AAA210909) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-Bub3 antibody
Bub3 is an adaptor protein and component of the spindle checkpoint machinery. Bub3 and other spindle checkpoint proteins ensure that segregation does not proceed until all replicated chromatids are associated with a functional bipolar spindle. Bub3 forms a constitutive complex with Bub1. Bub3 has been demonstrated to also function as a transcriptional repressor in association with Cdc20 and histone deacetylases.
Product Categories/Family for anti-Bub3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,955 Da
NCBI Official Full Name
mitotic checkpoint protein BUB3 isoform a
NCBI Official Synonym Full Names
BUB3 mitotic checkpoint protein
NCBI Official Symbol
BUB3
NCBI Official Synonym Symbols
BUB3L; hBUB3
NCBI Protein Information
mitotic checkpoint protein BUB3; mitotic checkpoint component; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog; BUB3 budding uninhibited by benzimidazoles 3 homolog
UniProt Protein Name
Mitotic checkpoint protein BUB3
UniProt Gene Name
BUB3
UniProt Entry Name
BUB3_HUMAN
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Product Notes
The Bub3 bub3 (Catalog #AAA210909) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Bub3 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bub3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Bub3 bub3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bub3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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