IF (Immunofluorescence)
(Immunofluorescence of bub3 in Jurkat cells with bub3 antibody at 20 μg/mL.)
Rabbit anti-Human, Mouse Bub3 Polyclonal Antibody | anti-BUB3 antibody
Bub3 Antibody
Gene Names
BUB3; BUB3L; hBUB3
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
Bub3 Antibody is affinity chromatography purified via peptide column.
Synonyms
Bub3, Antibody; Bub3 Antibody; Bub3; BUB3L; hBUB3; Mitotic checkpoint protein BUB3; budding uninhibited by benzimidazoles 3 homolog (yeast); anti-BUB3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Bub3 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
328
Applicable Applications for anti-BUB3 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Conjugate
Unconjugated
Immunogen
Bub3 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human bub3.
Buffer
Bub3 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
Bub3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
IF (Immunofluorescence)
(Immunofluorescence of bub3 in Jurkat cells with bub3 antibody at 20 μg/mL.)
IF (Immunofluorescence)
(Immunofluorescence of bub3 in Jurkat cells with bub3 antibody at 20 μg/mL.)
ICC (Immunocytochemistry)
(Immunocytochemistry of Bub3 in Jurkat cells with Bub3 antibody at 10 μg/mL.)
ICC (Immunocytochemistry)
(Immunocytochemistry of Bub3 in Jurkat cells with Bub3 antibody at 10 μg/mL.)
WB (Western Blot)
(Western blot analysis of bub3 in Jurkat cell lysate with bub3 antibody at (A) 0.5 and (B) 1 μg/mL.)
WB (Western Blot)
(Western blot analysis of bub3 in Jurkat cell lysate with bub3 antibody at (A) 0.5 and (B) 1 μg/mL.)
Related Product Information for anti-BUB3 antibody
Bub3 Antibody: The mitotic checkpoint protein Bub3 is involved with the essential spindle checkpoint pathway which operates during early embryogenesis. Bub3 is important during G2 and early mitosis stages, permitting entry into mitosis depending upon the assembly state of microtubules, thus preventing premature sister chromatid separation, mis-segregation and aneuploidy. Bub3 contains four WD repeat domains and is required for the kinetochore localization of Bub1, a related kinase that is necessary for spindle assembly checkpoint function. Bub1 is able to autophosphorylate and can catalyze the phosphorylation of Bub3. Both Bub1 and Bub3 are mutually dependent for function. Altered Bub expression levels may significantly impair mitotic checkpoint function and is associated with tumor cell proliferation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
36,955 Da
NCBI Official Full Name
Mitotic checkpoint protein BUB3
NCBI Official Synonym Full Names
BUB3 mitotic checkpoint protein
NCBI Official Symbol
BUB3
NCBI Official Synonym Symbols
BUB3L; hBUB3
NCBI Protein Information
mitotic checkpoint protein BUB3; mitotic checkpoint component; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog; BUB3 budding uninhibited by benzimidazoles 3 homolog
UniProt Protein Name
Mitotic checkpoint protein BUB3
UniProt Gene Name
BUB3
UniProt Entry Name
BUB3_HUMAN
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Product Notes
The BUB3 bub3 (Catalog #AAA39375) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bub3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Bub3 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the BUB3 bub3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bub3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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