FCM/FACS (Flow Cytometry)
(CCNT1 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse CCNT1 Polyclonal Antibody | anti-CCNT1 antibody
CCNT1 Antibody (Center)
Gene Names
CCNT1; CCNT; CYCT1; HIVE1
Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CCNT1, Antibody; CCNT1 Antibody (Center); Cyclin-T1; CycT1; Cyclin-T; CCNT1; anti-CCNT1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CCNT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the Central region of human CCNT1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.35 (varies by lot)
Sequence Positions
253-281
Sequence Length
184
Applicable Applications for anti-CCNT1 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CCNT1 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CCNT1 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(CCNT1 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CCNT1 antibody detected the CCNT1 protein (arrow).)
WB (Western Blot)
(CCNT1 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CCNT1 antibody detected the CCNT1 protein (arrow).)
WB (Western Blot)
(CCNT1 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the CCNT1 antibody detected the CCNT1 protein (arrow).)
WB (Western Blot)
(CCNT1 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the CCNT1 antibody detected the CCNT1 protein (arrow).)
Related Product Information for anti-CCNT1 antibody
CCNT1 belongs to the highly
conserved cyclin family, whose members are characterized by a
dramatic periodicity in protein abundance through the cell cycle.
Cyclins function as regulators of CDK kinases. Different cyclins
exhibit distinct expression and degradation patterns which
contribute to the temporal coordination of each mitotic event. This
cyclin tightly associates with CDK9 kinase, and was found to be a
major subunit of the transcription elongation factor p-TEFb. The
kinase complex containing this cyclin and the elongation factor can
interact with, and act as a cofactor of human immunodeficiency
virus type 1 (HIV-1) Tat protein, and was shown to be both
necessary and sufficient for full activation of viral
transcription. This cyclin and its kinase partner were also found
to be involved in the phosphorylation and regulation of the
carboxy-terminal domain (CTD) of the largest RNA polymerase II
subunit.
conserved cyclin family, whose members are characterized by a
dramatic periodicity in protein abundance through the cell cycle.
Cyclins function as regulators of CDK kinases. Different cyclins
exhibit distinct expression and degradation patterns which
contribute to the temporal coordination of each mitotic event. This
cyclin tightly associates with CDK9 kinase, and was found to be a
major subunit of the transcription elongation factor p-TEFb. The
kinase complex containing this cyclin and the elongation factor can
interact with, and act as a cofactor of human immunodeficiency
virus type 1 (HIV-1) Tat protein, and was shown to be both
necessary and sufficient for full activation of viral
transcription. This cyclin and its kinase partner were also found
to be involved in the phosphorylation and regulation of the
carboxy-terminal domain (CTD) of the largest RNA polymerase II
subunit.
Product Categories/Family for anti-CCNT1 antibody
References
Moiola, C., et al. Cell Cycle 9(15):3119-3126(2010)
Schonichen, A., et al. Biochemistry 49(14):3083-3091(2010)
Czudnochowski, N., et al. J. Mol. Biol. 395(1):28-41(2010)
Kapasi, A.J., et al. J. Virol. 83(11):5904-5917(2009)
Cho, S., et al. EMBO J. 28(10):1407-1417(2009)
Schonichen, A., et al. Biochemistry 49(14):3083-3091(2010)
Czudnochowski, N., et al. J. Mol. Biol. 395(1):28-41(2010)
Kapasi, A.J., et al. J. Virol. 83(11):5904-5917(2009)
Cho, S., et al. EMBO J. 28(10):1407-1417(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80685
NCBI Official Full Name
cyclin-T1 isoform a
NCBI Official Synonym Full Names
cyclin T1
NCBI Official Symbol
CCNT1
NCBI Official Synonym Symbols
CCNT; CYCT1; HIVE1
NCBI Protein Information
cyclin-T1
UniProt Protein Name
Cyclin-T1
UniProt Gene Name
CCNT1
UniProt Synonym Gene Names
CycT1; Cyclin-T
UniProt Entry Name
CCNT1_HUMAN
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Product Notes
The CCNT1 ccnt1 (Catalog #AAA289627) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 253-281. The CCNT1 Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CCNT1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CCNT1 ccnt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCNT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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