WB (Western Blot)
(Detection of human Cyclin T1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-Cyclin T1 antibody AAA212191 (lot AAA212191-1) used for IP at 6 ug/mg lysate. Cyclin T1 was also used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human Cyclin T1 Polyclonal Antibody | anti-CCNT1 antibody
Rabbit anti-Cyclin T1 Antibody, Affinity Purified
Gene Names
CCNT1; CCNT; CYCT1; HIVE1
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Cyclin T1, Antibody; Rabbit anti-Cyclin T1 Antibody, Affinity Purified; CYCT1; cyclin-T1; HIVE1; MLLT10/CCNT1 fusion; cyclin C-related protein; CDK9-associated C-type protein; CCNT; human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1; Cyclin-T; Cyclin-T1; cyclin T1; anti-CCNT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-CCNT1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine, Horse, Chimpanzee
Immunogen
Between 425 and 475
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Cyclin T1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-Cyclin T1 antibody AAA212191 (lot AAA212191-1) used for IP at 6 ug/mg lysate. Cyclin T1 was also used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human Cyclin T1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug) and HEK293T (T; 50 ug) cells. Antibody: Affinity purified rabbit anti-Cyclin T1 antibody AAA212191 (lot AAA212191-1) used for IP at 6 ug/mg lysate. Cyclin T1 was also used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Cyclin T1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cyclin T1 antibody AAA212191 (lot AAA212191-1) used for IP at 6 ug/mg lysate. Cyclin T1 was also immunoprecipitated by rabbit anti-Cyclin T1 antibodies)
IP (Immunoprecipitation)
(Detection of human Cyclin T1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cyclin T1 antibody AAA212191 (lot AAA212191-1) used for IP at 6 ug/mg lysate. Cyclin T1 was also immunoprecipitated by rabbit anti-Cyclin T1 antibodies)
IHC (Immunohistochemistry)
(Detection of human Cyclin T1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cyclin T1 (Cat. No. AAA212191 lot 1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Cyclin T1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cyclin T1 (Cat. No. AAA212191 lot 1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-CCNT1 antibody
Cyclin T1 is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, Cyclin T1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60563].
Product Categories/Family for anti-CCNT1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,206 Da
NCBI Official Full Name
cyclin-T1 isoform a
NCBI Official Synonym Full Names
cyclin T1
NCBI Official Symbol
CCNT1
NCBI Official Synonym Symbols
CCNT; CYCT1; HIVE1
NCBI Protein Information
cyclin-T1
UniProt Protein Name
Cyclin-T1
UniProt Gene Name
CCNT1
UniProt Synonym Gene Names
CycT1; Cyclin-T
UniProt Entry Name
CCNT1_HUMAN
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Product Notes
The CCNT1 ccnt1 (Catalog #AAA212191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Cyclin T1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin T1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CCNT1 ccnt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin T1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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