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IHC (Immunohistochemistry)
(IHC of mouse kidney using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.)
Goat CD31 Polyclonal Antibody
Anti-CD31
Reactivity
Human, Rat, Mouse, Monkey and Canine proteins.
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Epitope Affinity Purified
Synonyms
CD31, Antibody; Anti-CD31; CD31/endoCAM; endoCAM; PECA1; PECAM1; PECAM-1; platelet/endothelial adhesion molecule 1 antibody; anti-CD31 antibody
Host
Goat
Reactivity
Human, Rat, Mouse, Monkey and Canine proteins.
Clonality
Polyclonal
Isotype
IgG
Specificity
Using PBM cell lysates detects a 130-140 kDa band by Western blot.
Purity/Purification
Epitope Affinity Purified
Form/Format
Aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Applicable Applications for anti-CD31 antibody
Western Blot (WB), Immunohistochemistry-Frozen (IHC-F), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2,000
IHC (F): 1:250-1:1,000
IHC (P): 1:250-1:1,000
IHC (F): 1:250-1:1,000
IHC (P): 1:250-1:1,000
Handling
The antibody solution should be gently mixed before use.
Immunogen
Purified recombinant peptide within residues 690 aa to the C-terminus of human CD31 produced in E. coli.
Preparation and Storage
For continuous use, store at 2-8°C for one-two days. For extended storage, store in -20°C freezer. Working dilution samples should be discarded if not used within 12 hours.
IHC (Immunohistochemistry)
(IHC of mouse kidney using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.)
IHC (Immunohistochemistry)
(IHC of mouse kidney using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.)
IHC (Immunohistchemistry)
(IHC of mouse lung using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.)
IHC (Immunohistchemistry)
(IHC of mouse lung using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.)
IHC (Immunohistochemistry)
(IHC of mouse spleen using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.)
IHC (Immunohistochemistry)
(IHC of mouse spleen using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.)
IHC (Immunohistochemistry)
(IHC of human breast carcinoma using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.)
IHC (Immunohistochemistry)
(IHC of human breast carcinoma using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.)
WB (Western Blot)
(Endogenous CD31 detected at 1/500 dilution; lysate at 100 ug per lane and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
WB (Western Blot)
(Endogenous CD31 detected at 1/500 dilution; lysate at 100 ug per lane and rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
IHC (Immunohistochemistry)
(IHC of human lymph node using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.)
IHC (Immunohistochemistry)
(IHC of human lymph node using anti-CD31 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD31 Ab at 1:750/DAB detection.)
Application Data
Application Data
Related Product Information for anti-CD31 antibody
Goat polyclonal antibody to CD31. CD31 is a 130-140 kDa type I transmembrane glycoprotein. It is expressed in platelets, granulocytes, stem cells of myeloid lineage, monocytes and endothelial cells. It is likely to be involved in integrin activation, leukocyte migration and angiogenesis.
Similar Products
Product Notes
The CD31 (Catalog #AAA13874) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD31 reacts with Human, Rat, Mouse, Monkey and Canine proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's CD31 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Frozen (IHC-F), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2,000 IHC (F): 1:250-1:1,000 IHC (P): 1:250-1:1,000. Researchers should empirically determine the suitability of the CD31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD31, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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