WB (Western Blot)
(Detection of human CDCA2 by western blot. Samples: Whole cell lysate (50 ug) from K-562, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDCA2 antibody (AAA213473 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human CDCA2 Polyclonal Antibody | anti-CDCA2 antibody
Rabbit anti-CDCA2 Antibody, Affinity Purified
Gene Names
CDCA2; Repo-Man
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CDCA2, Antibody; Rabbit anti-CDCA2 Antibody, Affinity Purified; Repo-Man; cell division cycle-associated protein 2; PPP1R81; protein phosphatase 1, regulatory subunit 81; recruits PP1 onto mitotic chromatin at anaphase protein; Cell division cycle-associated protein 2; cell division cycle associated 2; anti-CDCA2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CDCA2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 200 and 250
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CDCA2 by western blot. Samples: Whole cell lysate (50 ug) from K-562, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDCA2 antibody (AAA213473 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human CDCA2 by western blot. Samples: Whole cell lysate (50 ug) from K-562, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDCA2 antibody (AAA213473 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human CDCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDCA2 antibody (AAA213473 lot 1) used for IP at 1 ug per reaction. CDCA2 was also immunoprecipitated by rabbit anti-CDCA2 antibody BL24283. For blotting immunoprecipitated CDCA2, AAA213473 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CDCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDCA2 antibody (AAA213473 lot 1) used for IP at 1 ug per reaction. CDCA2 was also immunoprecipitated by rabbit anti-CDCA2 antibody BL24283. For blotting immunoprecipitated CDCA2, AAA213473 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-CDCA2 antibody
CDCA2 is a regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q69YH5].
Product Categories/Family for anti-CDCA2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
112,676 Da
NCBI Official Full Name
cell division cycle-associated protein 2
NCBI Official Synonym Full Names
cell division cycle associated 2
NCBI Official Symbol
CDCA2
NCBI Official Synonym Symbols
Repo-Man
NCBI Protein Information
cell division cycle-associated protein 2; recruits PP1 onto mitotic chromatin at anaphase protein; protein phosphatase 1 [gamma] (PPP1R3A) binding protein Repo-Man
UniProt Protein Name
Cell division cycle-associated protein 2
UniProt Gene Name
CDCA2
UniProt Synonym Gene Names
Repo-Man
UniProt Entry Name
CDCA2_HUMAN
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Product Notes
The CDCA2 cdca2 (Catalog #AAA213473) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CDCA2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDCA2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CDCA2 cdca2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDCA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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