Rabbit Connexin 43 Polyclonal Antibody | anti-GJA1 antibody

Connexin 43 Antibody (N-term)

Cat. Num. AAA286929

• Gene Names: GJA1; HSS; CMDR; CX43; GJAL; ODDD; AVSD3; HLHS1
• Reactivity: Human, mouse, rat (Predicted Reactivity: Bovine, Xenopus)
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Synonyms: Connexin 43, Antibody; Connexin 43 Antibody (N-term); Gap junction alpha-1 protein; Connexin-43; Cx43; Gap junction 43 kDa heart protein; GJA1; GJAL; anti-GJA1 antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat (Predicted Reactivity: Bovine, Xenopus)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This Connexin 43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-138 amino acids from the N-terminal region of human Connexin 43.
• Purity/Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 107-138
• Sequence Length: 382

• Applicable Applications for anti-GJA1 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).
• Cellular Location: Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. Endoplasmic reticulum {ECO:0000250|UniProtKB:P23242}. Note=Localizes at the intercalated disk (ICD) in cardiomyocytes and the proper localization at ICD is dependent on TMEM65. {ECO:0000250|UniProtKB:P23242}
• Tissue Location: Expressed in the heart and fetal cochlea.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(Anti-GJA1 Antibody (N121) at 1:2000 dilution + C6 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 43 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

((LEFT)Western blot analysis of anti-GJA1 Antibody (N-term) in CEM cell line lysates (35ug/lane). GJA1(arrow) was detected using the purified Pab.(RIGHT) Western blot analysis of hGJA1-N121. Connexin in mouse brain tissue lysates (35ug/lane).GJA1 (arrow) was detected using the purified Pab.)

WB (Western Blot)

(Western blot analysis of lysates from Hela, U-87 MG, C6 cell line and mouse heart tissue lysate (from left to right), using GJA1 Antibody (N121). AAA286929 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Related Product Information for anti-GJA1 antibody

Gap junction protein, alpha 1 is a member of the connexin gene family and a component of gap junctions. Gap junctions are composed of arrays of intercellular channels and provide a route for the diffusion of materials of low molecular weight from cell to cell. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5.

Product Categories/Family for anti-GJA1 antibody

Crown Antibodies; Cardiovascular; Signal Transduction

References

Spinella, F., et al., J. Biol. Chem. 278(42):41294-41301 (2003). Contreras, J.E., et al., Proc. Natl. Acad. Sci. U.S.A. 100(20):11388-11393 (2003). Qin, H., et al., J. Biol. Chem. 278(32):30005-30014 (2003). Cameron, S.J., et al., J. Biol. Chem. 278(20):18682-18688 (2003). Ma, X.D., et al., World J. Gastroenterol. 9(5):946-950 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4504001
• NCBI GeneID: 2697
• NCBI Accession #: NP_000156.1
• NCBI GenBank Nucleotide #: NM_000165.4
• UniProt Accession #: P17302; Q6FHU1; Q9Y5I8; B2R5U9
• NCBI Official Full Name: gap junction alpha-1 protein
• NCBI Official Synonym Full Names: gap junction protein, alpha 1, 43kDa
• NCBI Official Symbol: GJA1
• NCBI Official Synonym Symbols: HSS; CMDR; CX43; GJAL; ODDD; AVSD3; HLHS1
• NCBI Protein Information: gap junction alpha-1 protein
• UniProt Protein Name: Gap junction alpha-1 protein
• UniProt Gene Name: GJA1
• UniProt Synonym Gene Names: GJAL; Cx43
• UniProt Entry Name: CXA1_HUMAN