FCM/FACS (Flow Cytometry)
(HMG1L10 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human HMG1L10 Polyclonal Antibody | anti-HMGB1 antibody
HMG1L10 Antibody (N-term)
Gene Names
HMGB1; HMG1; HMG3; SBP-1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
HMG1L10, Antibody; HMG1L10 Antibody (N-term); High mobility group protein B1; High mobility group protein 1; HMG-1; HMGB1; HMG1; anti-HMGB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This HMG1L10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-36 amino acids from the N-terminal region of human HMG1L10.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
9-36
Sequence Length
215
Applicable Applications for anti-HMGB1 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(HMG1L10 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(HMG1L10 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(HMG1L10 Antibody (N-term) western blot analysis in K562,Jurkat cell line lysates (35ug/lane).This demonstrates the HMG1L10 antibody detected the HMG1L10 protein (arrow).)
WB (Western Blot)
(HMG1L10 Antibody (N-term) western blot analysis in K562,Jurkat cell line lysates (35ug/lane).This demonstrates the HMG1L10 antibody detected the HMG1L10 protein (arrow).)
Related Product Information for anti-HMGB1 antibody
HMGB1L10 binds preferentially single-stranded DNA and unwinds double stranded DNA (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24894
NCBI Official Full Name
high mobility group protein B1
NCBI Official Synonym Full Names
high mobility group box 1
NCBI Official Symbol
HMGB1
NCBI Official Synonym Symbols
HMG1; HMG3; SBP-1
NCBI Protein Information
high mobility group protein B1
UniProt Protein Name
High mobility group protein B1
UniProt Gene Name
HMGB1
UniProt Synonym Gene Names
HMG1; HMG-1
UniProt Entry Name
HMGB1_HUMAN
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Product Notes
The HMGB1 hmgb1 (Catalog #AAA283787) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 9-36. The HMG1L10 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HMG1L10 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the HMGB1 hmgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMG1L10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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