Immunogen
(Immunogen: Recombinant protein representing aa 2-120 of human HMGB1 (green) depicted within in the full length structure prediction (blue) via AlphaFold. (https://alphafold.ebi.ac.uk/).)
Rabbit anti-Human HMGB1 Polyclonal Antibody | anti-HMGB1 antibody
HMGB1 Polyclonal Antibody
Reactivity
Human
Applications
Western Blot
Purity
Protein A purified
Synonyms
HMGB1, Antibody; HMGB1 Polyclonal Antibody; High mobility group protein B1; anti-HMGB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Protein A purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide.
Concentration
1 mg/mL (varies by lot)
Applicable Applications for anti-HMGB1 antibody
WB (Western Blot)
Immunogen
Recombinant Human HMGB1 aa 2-120
Preparation and Storage
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Immunogen
(Immunogen: Recombinant protein representing aa 2-120 of human HMGB1 (green) depicted within in the full length structure prediction (blue) via AlphaFold. (https://alphafold.ebi.ac.uk/).)
Immunogen
(Immunogen: Recombinant protein representing aa 2-120 of human HMGB1 (green) depicted within in the full length structure prediction (blue) via AlphaFold. (https://alphafold.ebi.ac.uk/).)
WB (Western Blot)
(20 ug of the indicated human whole cell extract was loaded onto a 4-20% SDS-PAGE gel. After electrophoresis, proteins were transferred to a PVDF membrane and probed with 3 ug/mL of antibody. Secondary detection was performed with 0.08 ug/mL of HRP-conjugated donkey anti-rabbit IgG antibody. Images were captured after development of membrane following exposure to AvivaBlot™ Femto ECL Reagent)
WB (Western Blot)
(20 ug of the indicated human whole cell extract was loaded onto a 4-20% SDS-PAGE gel. After electrophoresis, proteins were transferred to a PVDF membrane and probed with 3 ug/mL of antibody. Secondary detection was performed with 0.08 ug/mL of HRP-conjugated donkey anti-rabbit IgG antibody. Images were captured after development of membrane following exposure to AvivaBlot™ Femto ECL Reagent)
Related Product Information for anti-HMGB1 antibody
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
NCBI and Uniprot Product Information
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Product Notes
The HMGB1 (Catalog #AAA201888) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HMGB1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HMGB1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the HMGB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMGB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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