Rabbit HSP60 (Heat Shock Protein 60) Polyclonal Antibody | anti-HSP60 antibody

HSP60 (Heat Shock Protein 60)

Cat. Num. AAA76739

• Gene Names: HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• Reactivity: Human, Mouse, Rat, Primate, Bovine, Dog, Porcine, Equine
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
• Synonyms: HSP60 (Heat Shock Protein 60), Antibody; HSP60 (Heat Shock Protein 60); anti-HSP60 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Primate, Bovine, Dog, Porcine, Equine
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Form/Format: Liquid. Supplied as an aliquot of serum plus 5mM NaN3

• Applicable Applications for anti-HSP60 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
• Immunogen: Recombinant full length human HSP60 expressed in and purified from E coli
• Preparation and Storage: Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for sixmonths without detectable loss of activity. The antibody can be stored at 2 degree - 8 degree C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of different tissue or cell lysates using rabbit pAb to HSP-60, AAA76739, dilution 1:5,000 in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong 60kDa band present in all preparations corresponds to HSP60 protein.)

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of HeLa cells stained with rabbit pAb to HSP60, AAA76739, dilution 1:1,000, in red, and costained with chicken pAb to vimentin in green. The blue is DAPI staining of nuclear DNA. The HSP60 antibody gives strong and specific staining of mitochondria while the vimentin antibody reveals cytoplasmic intermediate filaments.)

Related Product Information for anti-HSP60 antibody

The heat shock proteins were discovered, as the name suggests, since they are heavily upregulated when cells are stressed by temperatures above the normal physiological range. They are expressed in unstressed cells also and have a normal function as chaperones, helping other proteins to fold correctly. The need for chaperones is much greater if a cell or tissue is stressed by heat, and so these proteins become heavily up regulated.. In addition to its role as a heat shock protein, HSP60 plays an important role in the transport and maintenance of mitochondrial proteins as well as the transmission and replication of mitochondrial DNA. HSP60 has also been implicated in the initiation and/or progression of some subtypes of cardiovascular disease (CVD).

NCBI and Uniprot Product Information

• NCBI GI #: 31542947
• NCBI GeneID: 3329
• NCBI Accession #: NP_002147.2
• NCBI GenBank Nucleotide #: NM_002156.4
• UniProt Accession #: P10809; Q38L19; Q9UCR6; B2R5M6
• Molecular Weight: 61,055 Da
• NCBI Official Full Name: 60 kDa heat shock protein, mitochondrial
• NCBI Official Synonym Full Names: heat shock 60kDa protein 1 (chaperonin)
• NCBI Official Symbol: HSPD1
• NCBI Official Synonym Symbols: HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• NCBI Protein Information: 60 kDa heat shock protein, mitochondrial; chaperonin 60; 60 kDa chaperonin; heat shock protein 65; P60 lymphocyte protein; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1
• UniProt Protein Name: 60 kDa heat shock protein, mitochondrial
• UniProt Gene Name: HSPD1
• UniProt Synonym Gene Names: HSP60; CPN60; HSP-60; Hsp60
• UniProt Entry Name: CH60_HUMAN