Rabbit anti-Human MEK1 Polyclonal Antibody | anti-MEK1 antibody

anti-MEK1 Antibody

Cat. Num. AAA297743

• Gene Names: MAP2K1; MEK1; MKK1; MAPKK1; PRKMK1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MEK1, Antibody; anti-MEK1 Antibody; Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; 2.7.12.2; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K1 (HGNC:6840); MEK1; PRKMK1; R03487NP; anti-MEK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This anti-MEK1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human anti-MEK1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

• Applicable Applications for anti-MEK1 antibody: WB (Western Blot)
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• HGNC: 6840
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(All lanes: Anti-anti-MEK1 Antibody at 1:500 dilution + A2058 whole cell lysateLysates/proteins at 20 ug per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 43 KDaBlocking/Dilution buffer: 5% NFDM/TBST.Dilution: WB 1:500)

WB (Western Blot)

(All lanes: Anti-anti-MEK1 Antibody at 1:1000 dilutionLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: A2058 whole cell lysateLysates/proteins at 20 ug per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 43 KDaBlocking/Dilution buffer: 5% NFDM/TBST.Dilution: WB 1:1000)

Related Product Information for anti-MEK1 antibody

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed:29433126). Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

Product Categories/Family for anti-MEK1 antibody

New Antibodies; Primary Antibodies; Developmental Biology; Signal Transduction

References

Seger R.,et al.J. Biol. Chem. 267:25628-25631 (1992).Zheng C.-F.,et al.J. Biol. Chem. 268:11435-11439 (1993).Stewart S.,et al.Mol. Cell. Biol. 19:5523-5534 (1999).Zheng C.-F.,et al.EMBO J. 13:1123-1131 (1994).Duesbery N.S.,et al.Science 280:734-737 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 5579478
• NCBI GeneID: 5604
• NCBI Accession #: NP_002746.1
• NCBI GenBank Nucleotide #: NM_002755.3
• UniProt Accession #: Q02750
• Molecular Weight: 43,439 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 1
• NCBI Official Symbol: MAP2K1
• NCBI Official Synonym Symbols: MEK1; MKK1; MAPKK1; PRKMK1
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 1; MEK 1; MAPKK 1; MAPK/ERK kinase 1; ERK activator kinase 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 1
• UniProt Gene Name: MAP2K1
• UniProt Synonym Gene Names: MEK1; PRKMK1; MAP kinase kinase 1; MAPKK 1; MKK1; MEK 1
• UniProt Entry Name: MP2K1_HUMAN