IHC (Immunohiostchemistry)
(Anti-NF-kB p65 antibody, AAA45068, IHC(P)IHC(P): Human Mammary Cancer Tissue)
Rabbit NF-kB p65 Polyclonal Antibody | anti-RELA antibody
Anti-NF-kB p65 antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
NF-kB p65, Antibody; Anti-NF-kB p65 antibody; Transcription factor p65; v-rel reticuloendotheliosis viral oncogene homolog A(avian); Avian reticuloendotheliosis viral(v rel) oncogene homolog A antibody; MGC131774 antibody; NF kappa B p65delta3 antibody; NFKB 3 antibody; NFKB3 antibody; Nuclear Factor NF Kappa B p65 Subunit antibody; Nuclear factor NF-kappa-B p65 subunit antibody; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody; OTTHUMP00000233473 antibody; OTTHUMP00000233474 antibody; OTTHUMP00000233475 antibody; OTTHUMP00000233476 antibody; OTTHUMP00000233900 antibody; p65 antibody; p65 NF kappaB antibody; p65 NFkB antibody; RELA antibody; TF65_HUMAN antibody; Transcription Factor p65 antibody; v rel avian reticuloendotheliosis viral oncogene homolog A(nuclear factor of kappa light polypeptide gene enhancer in B cells 3(p65)) antibody; V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A antibody; v rel reticuloendotheliosis viral oncogene homolog A(avian) antibody; V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody; v-rel reticuloendotheliosis viral oncogene homolog A antibody; anti-RELA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
551
Applicable Applications for anti-RELA antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human NF-kB p65 (143-158aa VPIEEQRGDYDLNAVR), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti-NF-kB p65 antibody, AAA45068, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti-NF-kB p65 antibody, AAA45068, IHC(P)IHC(P): Human Mammary Cancer Tissue)
WB (Western Blot)
(Anti-NF-kB p65 antibody, AAA45068, Western blottingLane 1: Rat Testis Tissue LysateLane 2: HELA Cell LysateLane 3: A431 Cell LysateLane 4: JURKAT Cell Lysate)
WB (Western Blot)
(Anti-NF-kB p65 antibody, AAA45068, Western blottingLane 1: Rat Testis Tissue LysateLane 2: HELA Cell LysateLane 3: A431 Cell LysateLane 4: JURKAT Cell Lysate)
Related Product Information for anti-RELA antibody
Description: Rabbit IgG polyclonal antibody for Transcription factor p65(RELA) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: RELA(V-REL AVIAN RETICULOENDOTHELIOSIS VIRAL ONCOGENE HOMOLOG A), also called NFKB3 or NFKB, p65 SUBUNIT. NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins, which inactivate NFKB by trapping it in the cytoplasm. The p65(RELA) heterodimer is the most abundant form of NFKB. And the RELA gene is located on 11q13.1. RELA is a nonhistone substrate of HDAC3 and that IKBA-dependent nuclear export of the HDAC3-deacetylated RELA replenishes the depleted cytoplasmic pool of latent NFKB-IKBA complexes for subsequent NFKB responses. RELA nucleocytoplasmic redistribution coincided with export of PPARG, and immunoprecipitation analysis indicated that PPARG-RELA association was dependent on the PPARG C-terminal ligand-binding domain. IKK-dependent phosphorylation of RELA on ser468 enhanced binding of GCN5 to RELA and RELA ubiquitination.
Background: RELA(V-REL AVIAN RETICULOENDOTHELIOSIS VIRAL ONCOGENE HOMOLOG A), also called NFKB3 or NFKB, p65 SUBUNIT. NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins, which inactivate NFKB by trapping it in the cytoplasm. The p65(RELA) heterodimer is the most abundant form of NFKB. And the RELA gene is located on 11q13.1. RELA is a nonhistone substrate of HDAC3 and that IKBA-dependent nuclear export of the HDAC3-deacetylated RELA replenishes the depleted cytoplasmic pool of latent NFKB-IKBA complexes for subsequent NFKB responses. RELA nucleocytoplasmic redistribution coincided with export of PPARG, and immunoprecipitation analysis indicated that PPARG-RELA association was dependent on the PPARG C-terminal ligand-binding domain. IKK-dependent phosphorylation of RELA on ser468 enhanced binding of GCN5 to RELA and RELA ubiquitination.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
59,910 Da
NCBI Official Full Name
Transcription factor p65
NCBI Official Synonym Full Names
v-rel avian reticuloendotheliosis viral oncogene homolog A
NCBI Official Symbol
RELA
NCBI Official Synonym Symbols
p65; NFKB3
NCBI Protein Information
transcription factor p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; v-rel reticuloendotheliosis viral oncogene homolog A; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
UniProt Protein Name
Transcription factor p65
UniProt Gene Name
RELA
UniProt Synonym Gene Names
NFKB3
UniProt Entry Name
TF65_HUMAN
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Product Notes
The RELA rela (Catalog #AAA45068) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NF-kB p65 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NF-kB p65 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the RELA rela for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF-kB p65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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