DB (Dot Blot)
(Dot blot analysis of anti-phospho-EphA2-pS897 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Rabbit Phospho-EphA2 (S897) Polyclonal Antibody | anti-EPHA2 antibody
Phospho-EphA2 (S897) Antibody
Gene Names
EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6
Reactivity
Human (Predicted Reactivity: Monkey, Mouse)
Applications
ELISA, Dot Blot, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-EphA2 (S897), Antibody; Phospho-EphA2 (S897) Antibody; Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; EPHA2; ECK; anti-EPHA2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Monkey, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EphA2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S897 of human EphA2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.35 (varies by lot)
Sequence Length
497
Applicable Applications for anti-EPHA2 antibody
ELISA, DB (Dot Blot), WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
DB (Dot Blot)
(Dot blot analysis of anti-phospho-EphA2-pS897 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
DB (Dot Blot)
(Dot blot analysis of anti-phospho-EphA2-pS897 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
WB (Western Blot)
(Western blot analysis of EphA2 Antibody (pS897) Pab (RB21717) pre-incubated without(lane 1) and with(lane 2) FBS in HA cell line lysate. EphA2 Antibody (pS897) (arrow) was detected using the purified Pab(Kindly provided by Dr. John Wu).)
WB (Western Blot)
(Western blot analysis of EphA2 Antibody (pS897) Pab (RB21717) pre-incubated without(lane 1) and with(lane 2) FBS in HA cell line lysate. EphA2 Antibody (pS897) (arrow) was detected using the purified Pab(Kindly provided by Dr. John Wu).)
WB (Western Blot)
(Western blot analysis of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml, using phospho-EphA2 Antibody (pS897) (upper) or Beta-actin (lower).)
WB (Western Blot)
(Western blot analysis of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml, using phospho-EphA2 Antibody (pS897) (upper) or Beta-actin (lower).)
Related Product Information for anti-EPHA2 antibody
EphA2 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands.
Product Categories/Family for anti-EPHA2 antibody
References
Larsen, A.B., et al. Cell. Signal. 22(4):636-644(2010)
Salaita, K., et al. Science 327(5971):1380-1385(2010)
Zhuang, G., et al. Cancer Res. 70(1):299-308(2010)
Salaita, K., et al. Science 327(5971):1380-1385(2010)
Zhuang, G., et al. Cancer Res. 70(1):299-308(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
108266
NCBI Official Full Name
ephrin type-A receptor 2
NCBI Official Synonym Full Names
EPH receptor A2
NCBI Official Symbol
EPHA2
NCBI Official Synonym Symbols
ECK; CTPA; ARCC2; CTPP1; CTRCT6
NCBI Protein Information
ephrin type-A receptor 2
UniProt Protein Name
Ephrin type-A receptor 2
UniProt Gene Name
EPHA2
UniProt Synonym Gene Names
ECK
UniProt Entry Name
EPHA2_HUMAN
Similar Products
Product Notes
The EPHA2 epha2 (Catalog #AAA290118) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-EphA2 (S897) Antibody reacts with Human (Predicted Reactivity: Monkey, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-EphA2 (S897) can be used in a range of immunoassay formats including, but not limited to, ELISA, DB (Dot Blot), WB (Western Blot). Researchers should empirically determine the suitability of the EPHA2 epha2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-EphA2 (S897), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.