Rabbit anti-Human PRKAR1A Polyclonal Antibody | anti-PRKAR1A antibody

PRKAR1A Polyclonal Antibody

Cat. Num. AAA36653

• Gene Names: PRKAR1A; CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Affinity Purification
• Synonyms: PRKAR1A, Antibody; PRKAR1A Polyclonal Antibody; PRKAR1A; CAR; CNC; CNC1; DKFZp779L0468; MGC17251; PKR1; PPNAD1; PRKAR1; TSE1; anti-PRKAR1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 381

• Applicable Applications for anti-PRKAR1A antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human PRKAR1A
• Calculated Molecular Weight: 43kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human oophoroma using PRKAR1A antibody at dilution of 1:100 (40x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using PRKAR1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Related Product Information for anti-PRKAR1A antibody

The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation (1). Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer. In this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (C-alpha, C-beta, and C-gamma) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. The two R families exist in two isoforms, alpha and beta (RI-alpha, RI-beta, RII-alpha, and RII-beta). Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC, which are characterized by an arginine at position -3 relative to the phosphorylated serine or threonine residue (2). Substrates that present this consensus sequence and have been shown to be phosphorylated by PKA are Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3alpha Ser21 and GSK-3beta Ser9) (3-5). In addition, combined knock-down of PKA C-alpha and -beta blocks cAMP-mediated phosphorylation of Raf (Ser43 and Ser259) (6). Autophosphorylation and phosphorylation by PDK-1 are two known mechanisms responsible for phosphorylation of the C subunit at Thr197 (7).

Product Categories/Family for anti-PRKAR1A antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 125193
• NCBI GeneID: 5573
• UniProt Accession #: P10644; Q567S7; K7ER48
• Molecular Weight: 381
• NCBI Official Full Name: cAMP-dependent protein kinase type I-alpha regulatory subunit
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, regulatory, type I, alpha
• NCBI Official Symbol: PRKAR1A
• NCBI Official Synonym Symbols: CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
• NCBI Protein Information: cAMP-dependent protein kinase type I-alpha regulatory subunit; Carney complex type 1; tissue-specific extinguisher 1; protein kinase A type 1a regulatory subunit; cAMP-dependent protein kinase regulatory subunit RIalpha; cAMP-dependent protein kinase type I-alpha regulatory chain
• UniProt Protein Name: cAMP-dependent protein kinase type I-alpha regulatory subunit
• UniProt Gene Name: PRKAR1A
• UniProt Synonym Gene Names: PKR1; PRKAR1; TSE1; TSE1
• UniProt Entry Name: KAP0_HUMAN