Rabbit anti-Human Protein Kinase A regulatory subunit I alpha Polyclonal Antibody | anti-PRKAR1A antibody

Protein Kinase A regulatory subunit I alpha Antibody (N-term)

Cat. Num. AAA287662

• Gene Names: PRKAR1A; CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Protein Kinase A regulatory subunit I alpha, Antibody; Protein Kinase A regulatory subunit I alpha Antibody (N-term); cAMP-dependent protein kinase type I-alpha regulatory subunit; Tissue-specific extinguisher 1; TSE1; N-terminally processed; PRKAR1A; PKR1; PRKAR1; anti-PRKAR1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Protein Kinase A regulatory subunit I alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Protein Kinase A regulatory subunit I alpha.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 1-30
• Sequence Length: 337

• Applicable Applications for anti-PRKAR1A antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemisry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(Western blot analysis of PKR1 (arrow) using rabbit polyclonal hPKR1-M1. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKR1 gene (Lane 2).(2ug/ml))

WB (Western Blot)

(Western blot analysis of anti-PKR1 Antibody (N-term) in CEM cell line lysates (35ug/lane). PKR1 (arrow) was detected using the purified Pab.)

Related Product Information for anti-PRKAR1A antibody

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PKR1 is one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Functional null mutations in the gene cause Carney complex (CNC), an autosomal dominant multiple neoplasia syndrome. The gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2.

Product Categories/Family for anti-PRKAR1A antibody

Cancer; Metabolism; Signal Transduction

References

Gronholm, M., et al., J. Biol. Chem. 278(42):41167-41172 (2003).
Bertherat, J., et al., Cancer Res. 63(17):5308-5319 (2003).
Stergiopoulos, S.G., et al., FEBS Lett. 546(1):59-64 (2003).
Robinson-White, A., et al., Hum. Mol. Genet. 12(13):1475-1484 (2003).
Holm, A.M., et al., J. Immunol. 170(11):5772-5777 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 443497964
• NCBI GeneID: 5573
• NCBI Accession #: NP_001263218.1; NP_001263219.1; NP_001265362.1; NP_002725.1; NP_997636.1; NP_997637.1
• NCBI GenBank Nucleotide #: NM_001276289.1
• UniProt Accession #: P10644; Q567S7; K7ER48
• Molecular Weight: 42982
• NCBI Official Full Name: cAMP-dependent protein kinase type I-alpha regulatory subunit isoform a
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, regulatory, type I, alpha
• NCBI Official Symbol: PRKAR1A
• NCBI Official Synonym Symbols: CAR; CNC; CNC1; PKR1; TSE1; ADOHR; PPNAD1; PRKAR1; ACRDYS1
• NCBI Protein Information: cAMP-dependent protein kinase type I-alpha regulatory subunit
• UniProt Protein Name: cAMP-dependent protein kinase type I-alpha regulatory subunit
• UniProt Gene Name: PRKAR1A
• UniProt Synonym Gene Names: PKR1; PRKAR1; TSE1; TSE1
• UniProt Entry Name: KAP0_HUMAN