IHC (Immunohiostchemistry)
(PRMT5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRMT5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit PRMT5 Polyclonal Antibody | anti-PRMT5 antibody
PRMT5 Antibody (N-term)
Gene Names
PRMT5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
Reactivity
Human (Predicted Reactivity: Bovine, Monkey)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PRMT5, Antibody; PRMT5 Antibody (N-term); Protein arginine N-methyltransferase 5; 211-; 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog; SKB1 homolog; SKB1Hs; N-terminally processed; PRMT5; HRMT1L5; IBP72; JBP1; SKB1; anti-PRMT5 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Monkey)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PRMT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-104 amino acids from the N-terminal region of human PRMT5.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.41 (varies by lot)
Sequence Positions
76-104
Sequence Length
576
Applicable Applications for anti-PRMT5 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(PRMT5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRMT5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(PRMT5 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRMT5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(PRMT5 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PRMT5 antibody detected the PRMT5 protein (arrow).)
WB (Western Blot)
(PRMT5 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the PRMT5 antibody detected the PRMT5 protein (arrow).)
Related Product Information for anti-PRMT5 antibody
Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono-and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.
References
Aggarwal, P., et al. Cancer Cell 18(4):329-340(2010)
Rank, G., et al. Blood 116(9):1585-1592(2010)
Cesaro, E., et al. J. Biol. Chem. 284(47):32321-32330(2009)
Zhao, Q., et al. Nat. Struct. Mol. Biol. 16(3):304-311(2009)
Bruns, A.F., et al. Biol. Chem. 390(1):59-65(2009)
Rank, G., et al. Blood 116(9):1585-1592(2010)
Cesaro, E., et al. J. Biol. Chem. 284(47):32321-32330(2009)
Zhao, Q., et al. Nat. Struct. Mol. Biol. 16(3):304-311(2009)
Bruns, A.F., et al. Biol. Chem. 390(1):59-65(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72684
NCBI Official Full Name
protein arginine N-methyltransferase 5 isoform b
NCBI Official Synonym Full Names
protein arginine methyltransferase 5
NCBI Official Symbol
PRMT5
NCBI Official Synonym Symbols
JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
NCBI Protein Information
protein arginine N-methyltransferase 5
UniProt Protein Name
Protein arginine N-methyltransferase 5
UniProt Gene Name
PRMT5
UniProt Synonym Gene Names
HRMT1L5; IBP72; JBP1; SKB1; SKB1 homolog; SKB1Hs
UniProt Entry Name
ANM5_HUMAN
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Product Notes
The PRMT5 prmt5 (Catalog #AAA289485) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 76-104. The PRMT5 Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine, Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's PRMT5 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PRMT5 prmt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRMT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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