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IHC (Immunohistochemistry)
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IHC (Immunohistochemistry) (SH2D1A/SAP Antibody-Immunohistochemistry of paraffin-embedded human stomach cancer using SH2D1A antibody at dilution of 1:200 (400x lens).)

Rabbit SH2D1A/SAP Polyclonal Antibody | anti-SH2D1A antibody

SH2D1A/SAP Rabbit anti-Human Polyclonal Antibody

Gene Names
SH2D1A; LYP; SAP; XLP; DSHP; EBVS; IMD5; XLPD; MTCP1; XLPD1; SAP/SH2D1A
Reactivity
Mouse, Rat, Human
Applications
Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Affinity purified
Synonyms
SH2D1A/SAP; Polyclonal Antibody; SH2D1A/SAP Rabbit anti-Human Polyclonal Antibody; IHC-plus SH2D1A/SAP Antibody; SH2D1A; LYP; IMD5; SH2 domain protein 1A; SH2 domain containing 1A; XLP; XLPD; DSHP; Duncan disease SH2-protein; EBVS; Lymphoproliferative syndrome; SAP; SLAM-associated protein; anti-SH2D1A antibody
Ordering
Host
Rabbit
Reactivity
Mouse, Rat, Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human SH2D1A/SAP
Purity/Purification
Affinity purified
Form/Format
PBS, pH7.3, 0.02% Sodium Azide, 50% Glycerol
Applicable Applications for anti-SH2D1A antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
Application Notes
IF: 1:50-1:200
IHC: 1:50-1:200
IHC-P: 1:200
WB: 1:500-1:2000
The predicted MW is 6kDa/8kDa/13kDa/14kDa, while the observed MW by Western blot was 17kDa.
Target
Human SH2D1A/SAP
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human SH2D1A (NP_002342.1). MDAVAVYHGKISRETGEKLLLATGLDGSYLLRDSESVPGVYCLCVLYHGYIYTYRVSQTETGSWSAETAPGVHKRYFRKIKNLISAFQKPDQGIVIPLQYPVEKKSSARSTQGTTGIREDPDVCLKAP
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze-thaw cycles.

IHC (Immunohistochemistry)

(SH2D1A/SAP Antibody-Immunohistochemistry of paraffin-embedded human stomach cancer using SH2D1A antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistochemistry) (SH2D1A/SAP Antibody-Immunohistochemistry of paraffin-embedded human stomach cancer using SH2D1A antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistchemistry)

(SH2D1A/SAP Antibody-Immunohistochemistry of paraffin-embedded rat lung using SH2D1A antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistchemistry) (SH2D1A/SAP Antibody-Immunohistochemistry of paraffin-embedded rat lung using SH2D1A antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistochemistry)

(SH2D1A/SAP Antibody-Immunohistochemistry of paraffin-embedded human liver cancer tissue.)

IHC (Immunohistochemistry) (SH2D1A/SAP Antibody-Immunohistochemistry of paraffin-embedded human liver cancer tissue.)

IHC (Immunohistochemistry)

(SH2D1A/SAP Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (SH2D1A/SAP Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot)

(SH2D1A/SAP Antibody-Western blot analysis of extracts of various cell lines, using SH2D1A antibody.)

WB (Western Blot) (SH2D1A/SAP Antibody-Western blot analysis of extracts of various cell lines, using SH2D1A antibody.)

WB (Western Blot)

(SH2D1A/SAP Antibody-Western blot analysis of extracts of various cell lines, using SH2D1A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.)

WB (Western Blot) (SH2D1A/SAP Antibody-Western blot analysis of extracts of various cell lines, using SH2D1A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.)

ICC (Immunocytochemistry)

(SH2D1A/SAP Antibody-Immunofluorescence analysis of HeLa cell using SH2D1A antibody. Blue: DAPI for nuclear staining.)

ICC (Immunocytochemistry) (SH2D1A/SAP Antibody-Immunofluorescence analysis of HeLa cell using SH2D1A antibody. Blue: DAPI for nuclear staining.)
Related Product Information for anti-SH2D1A antibody
SAP antibody is an unconjugated rabbit polyclonal antibody to SAP (SH2D1A) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
SH2 domain-containing protein 1A isoform 1
NCBI Official Synonym Full Names
SH2 domain containing 1A
NCBI Official Symbol
SH2D1A
NCBI Official Synonym Symbols
LYP; SAP; XLP; DSHP; EBVS; IMD5; XLPD; MTCP1; XLPD1; SAP/SH2D1A
NCBI Protein Information
SH2 domain-containing protein 1A
UniProt Protein Name
SH2 domain-containing protein 1A
UniProt Gene Name
SH2D1A
UniProt Synonym Gene Names
DSHP; SAP; SLAM-associated protein
UniProt Entry Name
SH21A_HUMAN

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Product Notes

The SH2D1A sh2d1a (Catalog #AAA21302) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SH2D1A/SAP Rabbit anti-Human Polyclonal Antibody reacts with Mouse, Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's SH2D1A/SAP can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB). IF: 1:50-1:200 IHC: 1:50-1:200 IHC-P: 1:200 WB: 1:500-1:2000 The predicted MW is 6kDa/8kDa/13kDa/14kDa, while the observed MW by Western blot was 17kDa. Researchers should empirically determine the suitability of the SH2D1A sh2d1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SH2D1A/SAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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