Rabbit WNT8B / Wnt 8b Polyclonal Antibody | anti-WNT8B antibody

Rabbit Polyclonal to Human WNT8B / Wnt 8b

Cat. Num. AAA50821

• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: WNT8B / Wnt 8b, Antibody; Rabbit Polyclonal to Human WNT8B / Wnt 8b; Anti-WNT8B / Wnt 8b Antibody (Internal) IHC-plus; WNT8B; Protein Wnt-8b; WNT 8b; Human WNT8B; Wnt 8b; anti-WNT8B antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
• Clonality: Polyclonal
• Specificity: Human WNT8B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, less than 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 351

• Applicable Applications for anti-WNT8B antibody: IHC (Immunohistochemistry)
• Family/Subfamily: WNT/not assigned-WNT
• Immunogen Description: Synthetic 16 amino acid peptide from internal region of human WNT8B. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Dog, Bovine, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: WNT8B / Wnt 8b antibody was raised against synthetic 16 amino acid peptide from internal region of human WNT8B. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Dog, Bovine, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig (100%); Opossum, Platypus (94%); Marmoset (88%).
• Epitope: Internal
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store undiluted at -20 degree C or below for up to 1 year. Can be stored undiluted at 4 degree C for up to 1 month. Avoid freeze thaw cycles.

IHC (Immunohiostchemistry)

(Anti-WNT8B antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry)

(Anti-WNT8B antibody IHC of human brain, basal nucleus of Meynert. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-WNT8B antibody

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 95%, 86% and 71% amino acid identity to the mouse, zebrafish and Xenopus Wnt8B proteins, respectively. The expression patterns of the human and mouse genes appear identical and are restricted to the developing brain. The chromosomal location of this gene to 10q24 suggests it as a candidate gene for partial epilepsy.

Product Categories/Family for anti-WNT8B antibody

Family: WNT

NCBI and Uniprot Product Information

• NCBI GI #: 110735437
• NCBI GeneID: 7479
• NCBI Accession #: NP_003384.2
• NCBI GenBank Nucleotide #: NM_003393.3
• UniProt Accession #: Q93098; O00771; Q5VX55; Q8WYK9
• Molecular Weight: 38,721 Da
• NCBI Official Full Name: protein Wnt-8b
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 8B
• NCBI Official Symbol: WNT8B
• NCBI Protein Information: protein Wnt-8b
• UniProt Protein Name: Protein Wnt-8b
• UniProt Gene Name: WNT8B
• UniProt Entry Name: WNT8B_HUMAN