WB (Western Blot)
(PRMT / MEP50 complex, Activity Assay 0.5 ug Histone H4 was incubated with 0 ug (-), 0.225 ug (+), 0.45 ug (++), or 0.9 ug (+++) PRMT5 / MEP50 complex in 30 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 hours at room temperature. 6 ul reaction products were loaded and run on a 12.5% SDS-PAGE gel. Western Blot was used to detect the generation of reaction products (H4R3me2s antibody). PRMT5 / MEP50 Complex only was used as negative control.)
PRMT5/MEP50 recombinant protein
Recombinant PRMT5/MEP50 complex
Gene Names
PRMT5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
Synonyms
PRMT5/MEP50; N/A; Recombinant PRMT5/MEP50 complex; Protein Arginine Methyltransferase 5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5; PRMT5/MEP50 recombinant protein
Host
Baculovirus
Form/Format
Recombinant PRMT5/MEP50 Complex is supplied at a concentration of 0.4ug/ul in 25mM HEPES pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant PRMT5/MEP50 Complex is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions: 0.5ug Histone H4 was incubated with different concentrations of PRMT5/MEP50 Complex in 30ul reaction system including 50mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2mM MgCl2, 1mM TCEP, and 50uM SAM for 3 hours at room temperature. Activity was detected by Western Blot.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
WB (Western Blot)
(PRMT / MEP50 complex, Activity Assay 0.5 ug Histone H4 was incubated with 0 ug (-), 0.225 ug (+), 0.45 ug (++), or 0.9 ug (+++) PRMT5 / MEP50 complex in 30 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 hours at room temperature. 6 ul reaction products were loaded and run on a 12.5% SDS-PAGE gel. Western Blot was used to detect the generation of reaction products (H4R3me2s antibody). PRMT5 / MEP50 Complex only was used as negative control.)
WB (Western Blot)
(PRMT / MEP50 complex, Activity Assay 0.5 ug Histone H4 was incubated with 0 ug (-), 0.225 ug (+), 0.45 ug (++), or 0.9 ug (+++) PRMT5 / MEP50 complex in 30 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 hours at room temperature. 6 ul reaction products were loaded and run on a 12.5% SDS-PAGE gel. Western Blot was used to detect the generation of reaction products (H4R3me2s antibody). PRMT5 / MEP50 Complex only was used as negative control.)
SDS-PAGE
(Recombinant PRMT5 / MEP50 complex - protein gel. 10% SDS-PAGE gel stained with Coomassie blue. MW of PRMT5: 74 kDa MW of MEP50: 36.7 kDa Purity: > 90% )
SDS-PAGE
(Recombinant PRMT5 / MEP50 complex - protein gel. 10% SDS-PAGE gel stained with Coomassie blue. MW of PRMT5: 74 kDa MW of MEP50: 36.7 kDa Purity: > 90% )
Related Product Information for PRMT5/MEP50 recombinant protein
Short Description: Recombinant PRMT5/MEP50 complex includes full-length PRMT5 (accession numbers NP_006100.2) with an N-terminal DYKDDDDK tag and full-length MEP50 (accession numbers NP_077007.1) without a tag; it was expressed in a baculovirus expression system. The molecular weights of PRMT5 and MEP50 are 74 kDa and 36.7 kDa, respectively. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates Piwi proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. PRMT5 acts as part of a multimeric complex in concert with a variety of partner proteins that regulate its function and specificity. A core component of these complexes is the WD40 protein MEP50/WDR77/p44, which mediates interactions with binding partners and substrates. PRMT5 is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.
Background: PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates Piwi proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. PRMT5 acts as part of a multimeric complex in concert with a variety of partner proteins that regulate its function and specificity. A core component of these complexes is the WD40 protein MEP50/WDR77/p44, which mediates interactions with binding partners and substrates. PRMT5 is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.
Product Categories/Family for PRMT5/MEP50 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66,311 Da
NCBI Official Full Name
protein arginine N-methyltransferase 5 isoform a
NCBI Official Synonym Full Names
protein arginine methyltransferase 5
NCBI Official Symbol
PRMT5
NCBI Official Synonym Symbols
JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5
NCBI Protein Information
protein arginine N-methyltransferase 5; SKB1 homolog; jak-binding protein 1; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; shk1 kinase-binding protein 1 homolog; histone-arginine N-methyltransferase PRMT5
UniProt Protein Name
Protein arginine N-methyltransferase 5
UniProt Gene Name
PRMT5
UniProt Synonym Gene Names
HRMT1L5; IBP72; JBP1; SKB1; SKB1 homolog; SKB1Hs
UniProt Entry Name
ANM5_HUMAN
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Product Notes
The PRMT5/MEP50 prmt5 (Catalog #AAA59732) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PRMT5/MEP50, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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