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▼Recombinant Antibodies
Also available for purchase in our catalog, we have flexible recombinant antibodies that are excellent tools in many different types of experiments, and well-suited for those that are large-scale or long-term. Our collection of recombinant antibodies includes different forms/formats of said antibodies, such as labeled or purified versions, and can be made utilizing different species’ isotypes to suit various experimental requirements.
Viewing 2800-2819 of 2819 product results
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Hela cells stained with CSB-RA008790A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
FOS, Monoclonal Recombinant Antibody (Cat# AAA235541)
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells with AAA235549 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
GPC3, Monoclonal Recombinant Antibody (Cat# AAA235549)
IHC (Immunohiostchemistry)
(IHC image of CSB-RA010124A0HU diluted at 1:215 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
HAMP, Monoclonal Recombinant Antibody (Cat# AAA235553)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing MCF-7 cells stained with CSB-RA010605A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
HNRNPC, Monoclonal Recombinant Antibody (Cat# AAA235556)
IP (Immunoprecipitation)
(Immunoprecipitating HNRNPK in HepG2 whole cell lysateLane 1: Rabbit control IgG instead of CSB-RA010611A0HU in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA010611A0HU (3ug) + HepG2 whole cell lysate (500ug)Lane 3: HepG2 whole cell lysate (20ug))
HNRNPK, Monoclonal Recombinant Antibody (Cat# AAA235557)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing Jurkat cells stained with CSB-RA010802A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
HSP90AA1/HSP90AB1, Monoclonal Recombinant Antibody (Cat# AAA235559)
IP (Immunoprecipitation)
(Immunoprecipitating Hsp27 in Hela whole cell lysateLane 1: Rabbit control IgG instead of CSB-RA010833A0HU in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA010833A0HU (3ug) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (20ug))
HSPB1, Monoclonal Recombinant Antibody (Cat# AAA235560)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cells with CSB-RA011087A1HU at 1:29, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
HSP90AA1, Monoclonal Recombinant Antibody (Cat# AAA235564)
IF (Immunofluorescence)
(Immunofluorescence staining of A549 cells with CSB-RA011972A63phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
JUN, Monoclonal Recombinant Antibody (Cat# AAA235568)
IP (Immunoprecipitation)
(Immunoprecipitating NFKBIA in HepG2 whole cell lysateLane 1: Rabbit control IgG instead of CSB-RA015761A0HU in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA015761A0HU (3ug) + HepG2 whole cell lysate (500ug)Lane 3: HepG2 whole cell lysate (20ug))
NFKBIA, Monoclonal Recombinant Antibody (Cat# AAA235575)
IHC (Immunohiostchemistry)
(Immunochemical staining of human LCN2 in human lung with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
Lipocalin-2/NGAL, Monoclonal Recombinant Antibody (Cat# AAA258781)
WB (Western Blot)
(Western blot analysis of extracts from serum-starved MCF7, treated with IGF-1 (100 ng/mL, 30 min), using Phospho-S6K1 (Thr389) rabbit monoclonal Antibody (#AAA258873) and other brands’ antibodies (company C, company P) at dilution of 1:1000, 1:10000 and 1:200000.)
p70 S6 Kinase 1, Monoclonal Recombinant Antibody (Cat# AAA258873)
IF (Immunofluorescence)
(Immunofluorescence staining of Phospho-p38 MAPK (Thr180, Tyr182) in serum-starved NIH-3T3 cells, untreated (left), treated with UV (30mins) (middle) or treated with UV (30mins) and lambda phosphatase (right). Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Mouse Phospho-p38 MAPK (Thr180, Tyr182) monoclonal antibody (dilution ratio 1:60) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was mainly localized to Nucleus.)
p38 MAPK, Monoclonal Recombinant Antibody (Cat# AAA258875)
NHBA/Dextranase, Monoclonal Recombinant Antibody (Cat# AAA120371)
CA9, Monoclonal Recombinant Antibody (Cat# AAA120730)
Protein A/G purified from cell culture supernatant.
WB (Western Blot)
(Western blot analysis of extracts from serum-starved Hela, treated with Calyculin A (100 nM, 30 min), using Phospho-eIF2? (Ser51) Antibody (#AAA258877) and other brands’ antibodies (company C) at dilution of 1:1000, 1:10000 and 1:200000.)
eIF2alpha, Monoclonal Recombinant Antibody (Cat# AAA258877)
HLA-G1, Monoclonal Recombinant Antibody (Cat# AAA120733)
Protein A/G purified from cell culture supernatant.
IP (Immunoprecipitation)
(PCNA was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg MCF7 Whole Cell Lysate4 uL anti-PCNA rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PCNA rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 29 kDaObserved band size :35 kDa)
PCNA, Monoclonal Recombinant Antibody (Cat# AAA258901)
IHC (Immunohiostchemistry)
(Immunochemical staining of human IL-33 in human tonsil with rabbit monoclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)
IL-33, Monoclonal Recombinant Antibody (Cat# AAA258796)
What are recombinant antibodies?
Recombinant antibodies are produced in the lab by utilizing a known genetic sequence (an amino acid and nucleotide sequence). This allows for high consistency in how each new lot will perform and how specific they will be for the target, regardless of how many times they are produced/reproduced.
They help solve of the problems observed when traditional non-recombinant techniques are utilized, such as changes in cell behavior over time, differences between production batches, and accuracy in complex biological systems.
A powerful feature of recombinant antibodies is that they can also be modified through progressive genetic engineering and subsequent testing in order to make them perform better and/or more reliably.
Applications For Recombinant Antibodies
1. Basic Research
Recombinant antibodies are primarily utilized in laboratory experiments to study the function of various proteins in cells. They are helpful in finding and tracking specific molecules through their use in techniques such as ELISA, Western blotting, immunofluorescence, and flow cytometry.
2. Diagnostics
They are also key components in diagnostic tests used to detect diseases and infections. As recombinant antibodies are produced in a consistent and controlled way, they are able to provide highly reliable results across batches. Though recombinant antibodies are indeed quite useful in diagnostic applications, all of the products listed in AAA Biotech’s also known as AAA Bio or AAABio catalog are strictly for research-use only (RUO).
3. Therapeutics (Medical Treatments)
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
- Chimeric Antibodies: Commonly used in therapeutic treatment. They combine different pieces of mouse and human antibodies in order to reduce immune reactions.
- Humanized Antibodies: Modified non-human antibodies that are made to look the same as actual native human antibodies, making them safer and more effective.
- Antibody Fragments (Fab, scFv, VHH): Smaller pieces of antibodies that can reach deep into tissues and tumors. They are useful in cancer treatments and imaging.
- Bispecific Antibodies: Specially-engineered antibodies that can bind to two different target molecules at once. Each antibody has two “arms” that allow for two places where the target antigen can bind (both “arms” will recognize the same exact epitope). These bispecific antibodies are engineered so that each arm can detect a completely different epitope than the other. Used in many of the treatments for cancer, diabetes, Alzheimer’s disease, and more.
- Fc-Fusion Proteins: Used as drugs and research tools. They combine parts of antibodies with other proteins to improve stability and effectiveness.
4. Imaging and Detection
Small antibody fragments are also used as imaging agents in medical scans. Their small size helps them reach and report on the status of tumors quickly.
5. Drug Development and Delivery
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Recombinant antibodies are can also be designed and be made to deliver drugs directly to diseased cells, especially in targeted cancer therapy.
6. Immunotherapy
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
These antibodies can aid the immune system recognize and fight diseases more effectively, which is often their role in cancer treatment.
Advantages of Recombinant Antibodies
- Reliable Results Every Time: Recombinant antibodies are exceptionally consistent from batch to batch. This means researchers can trust that results will remain the same each time they purchase and use them.
- More Specific and Accurate: Compared to polyclonal antibodies, recombinant antibodies are significantly less likely to exhibit non-specific binding, which promotes more accurate and clearer results.
- Easier to Develop: Making recombinant antibodies is simpler and quicker than the traditional method of producing monoclonal antibodies via hybridomas.
- No Genetic Drift: Over time, hybridoma cells can change or become unstable, which can result in the antibodies progressively losing their function/specificity. Recombinant antibodies avoid this problem because they are produced from a fixed template DNA sequence.
- Truly Monoclonal: Studies have shown that many hybridomas thought to be monoclonal actually are not so, which can lead to, in some cases, unreliable results. Recombinant antibodies are made using precise genetic sequence templates, ensuring they are truly specific to one target.
- Long-Term Stability and Safety: Since their amino acid and genetic sequences are known and stored, recombinant antibodies can be reproduced any time with the same quality. This method protects against contamination, genetic changes over time, or even accidental loss/deterioration of cell lines.
Why Buy Recombinant Antibody from AAA Biotech?
- Highly Validated: The vast majority of our antibodies are thoroughly tested to ensure they work reliably across different applications and experimental platforms.
- Versatile Applications: Our antibodies can be used in a wide range of research techniques, including (but not limited to) immunocytochemistry (ICC), ELISA, immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), immunoprecipitation (IP), and Western blotting (WB).
- Support for Rare Species: We offer antibodies specifically designed for rare or less commonly used laboratory species — something many other suppliers do not provide.
- Wide Selection of Conjugated and Recombinant Forms: Choose from various labeled (conjugated) options and browse our high-quality recombinant antibodies – we are confident that we will have materials to meet your research needs.
FAQ
1. What are recombinant antibodies used for?
Research (e.g., ELISA, Western blot, flow cytometry)
Disease diagnostics
Therapeutic treatments (e.g., cancer, autoimmune diseases)
Medical imaging
Targeted drug delivery
Vaccine development
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
2. What is the difference between traditional and recombinant antibodies?
Traditional antibodies: Produced using animal hosts (hybridomas are typically made from an original animal inoculation), slower, less consistent, risk of genetic drift
Recombinant antibodies: Produced using DNA template sequences via laboratory cell lines, faster, highly consistent, animal-free, easily customizable
3. What are antibodies used to treat?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Antibodies are proteins that your body makes to protect you from infections, allergies, and other potentially harmful substances. Scientists have used the intrinsic properties of antibodies to develop them into tools for scientific advancement, and can now create special lab-grown antibodies that aid in the treatment of diseases such as cancer, heart-related issues, and arthritis.
4. Why do we need recombinant proteins?
Recombinant proteins are lab-made proteins that have become essential in the advancement of science and medicine. They’re used in the lab for innumerable purposes, such as to help grow and support cells, especially for tasks like expanding cell colonies, helping them develop into other types, or turning them into stem cells.