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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
At AAA Biotech, also known as AAA Bio or AAABio, we provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Whether you're conducting research on cell expansion, polarization, differentiation, or cell processing, we can offer the exact recombinant proteins you need in order to support your work.
Viewing 5350-5400 of 10000 product results
Application Data
LIMK1, Recombinant Protein (Cat# AAA73003)
Application Data
(The purity of MLL1 (KMT2A) was determined to be >90%by densitometry, approx. MW 52kDa.)
MLL1, Recombinant Protein (Cat# AAA73021)
Application Data
(The specific activity of TPM3-TRKA was determined to be 13 nmol /min/mg as per activity assay protocol.)
TPM3-TRKA, Recombinant Protein (Cat# AAA73035)
Application Data
FIP1L1-PDGFR alpha, Recombinant Protein (Cat# AAA73042)
Application Data
PIP5K3, Recombinant Protein (Cat# AAA73047)
Application Data
AMPK, Recombinant Protein (Cat# AAA73091)
Application Data
PDE4D, Recombinant Protein (Cat# AAA73102)
Application Data
GOPC-ROS1, Recombinant Protein (Cat# AAA73116)
Application Data
RhoA, Recombinant Protein (Cat# AAA73120)
Application Data
SETD7, Recombinant Protein (Cat# AAA73131)
Application Data
(The purity of SIRT2 was determined to be >70% by densitometry. Approx. MW 42kDa.)
SIRT2, Recombinant Protein (Cat# AAA73139)
Application Data
(The purity of SIRT7 was determined to be >90% by densitometry. Approx. MW 46kDa)
SIRT7, Recombinant Protein (Cat# AAA73143)
Application Data
(The purity of STAT5B protein was determined to be >95% by densitometry.Approx. MW 115kDa.)
STAT5B, Recombinant Protein (Cat# AAA73147)
Application Data
TGFBR1, Recombinant Protein (Cat# AAA73151)
Application Data
ULK2, Recombinant Protein (Cat# AAA73155)
Purity Data
SDS-PAGE
(SDS-PAGE gel image The purity of SARS-CoV Spike protein RBD was determined to be >90% by densitometry. Approx. MW 65 kDa.)
SARS-CoV Spike RBD, Recombinant Protein (Cat# AAA73182)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PKN1/PRK1, Recombinant Protein (Cat# AAA73868)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PKD2 (PRKD2), Recombinant Protein (Cat# AAA73876)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
CAMK2 beta, Recombinant Protein (Cat# AAA73307)
Bioactivity
(Activity: Sample activity data. For lot specific data please refer to the specific datasheet.)
COVID 19 Spike RBD (R346K, E484K, N501Y) Coronavirus, Recombinant Protein (Cat# AAA73342)
Bioactivity
(Activity: Sample activity data. For lot-specific data please refer to the lot-specific datasheet.)
COVID 19 Spike RBD (L452R) Coronavirus, Recombinant Protein (Cat# AAA73346)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
CDK9/CyclinT2, Recombinant Protein (Cat# AAA73359)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
FYN A, Recombinant Protein (Cat# AAA73573)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Bioactivity
(Human CD27 Ligand/TNFSF7 is coated at 10 ug/ml (100 ul/well) can bind Human CD27/TNFRSF7. The ED50 range )
CD27/TNFRSF7, Recombinant Protein (Cat# AAA48413)
Bioactivity
(Human Fc gamma RIIIA/CD16a is coated at 10 ug/ml (100 ul/well) can bind Human IgG1 Fc in a functional ELISA assay.)
Fc gamma RIIIA/CD16a, Recombinant Protein (Cat# AAA48425)
SDS-PAGE
alpha-Synuclein, Recombinant Protein (Cat# AAA48430)
SDS-PAGE
alpha-Synuclein A30P/A53T, Recombinant Protein (Cat# AAA48431)
SDS-PAGE
gamma-Synuclein, Recombinant Protein (Cat# AAA48433)
SDS-PAGE
CRYAB (Crystallin alpha B), Recombinant Protein (Cat# AAA48439)
SDS-PAGE
HSP 90, Recombinant Protein (Cat# AAA48440)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualised by coomassie blue stain.)
Vaspin, Recombinant Protein (Cat# AAA48464)
SDS-PAGE
(3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SIGLEC6, Recombinant Protein (Cat# AAA48965)
SDS-PAGE
(SDS-PAGE of endotoxin-free 70kDa native human Hsp70 protein (SPR-117).)
Hsp70, Recombinant Protein (Cat# AAA103774)
Application Data
(HTRF assay for JMJD1A / KDM3A activity 3 uM H3K9me1 peptide was incubated with JMJD1A / KDM3A in reaction buffer for 2 hours at room temperature. Anti-H3K9me0 antibody was used to detect reaction product.)
JMJD1A/KDM3A, Recombinant Protein (Cat# AAA59693)
WB (Western Blot)
(Recombinant NSD3 (WHSC1L1)-SET activity assay. 2 ug recombinant nucleosomes were incubated with NSD3 (WHSC1L1)-SET protein in 30 ul reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 h at RT. 5 ul reaction products were loaded on a 12.5% SDS-PAGE gel. Western Blot was used to detect reaction products (H3K36me2). NSD3 (WHSC1L1)-SET only was used as negative control. Results show that the activity of NSD3 (WHSC1L1)-SET with nucleosomes as substrate is much better than its activity with histone octamers as substrate.)
NSD3, Recombinant Protein (Cat# AAA59707)
Application Data
(HTRF assay for EHMT1 (894-1298) activity 3 uM histone peptide H3 (1-21) was incubated with EHMT1 (894-1298) in reaction buffer for 3 hours at room temperature. H3K9me2 antibody was used to detect reaction product. )
EHMT1, Recombinant Protein (Cat# AAA59731)
WB (Western Blot)
(PRMT / MEP50 complex, Activity Assay 0.5 ug Histone H4 was incubated with 0 ug (-), 0.225 ug (+), 0.45 ug (++), or 0.9 ug (+++) PRMT5 / MEP50 complex in 30 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 hours at room temperature. 6 ul reaction products were loaded and run on a 12.5% SDS-PAGE gel. Western Blot was used to detect the generation of reaction products (H4R3me2s antibody). PRMT5 / MEP50 Complex only was used as negative control.)
PRMT5/MEP50, Recombinant Protein (Cat# AAA59732)
Application Data
(HTRF assay for HDAC8 protein activity.3 uM Histone H3K9ac (1-21aa) peptide was incubated with HDAC8 protein in reaction buffer for 30 min at 37 degree C, Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.)
HDAC8, Recombinant Protein (Cat# AAA59741)
Application Data
(HDAC-Glo Class IIa Assay for HDAC9 activity.Assay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 uM substrate was incubated with HDAC9 proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)
HDAC9, Recombinant Protein (Cat# AAA59742)
Application Data
(HTRF assay for HDAC11 (2-347) protein activity 1 uM histone peptide H3K9ac (1-21) was incubated with HDAC11 (2-347) in reaction buffer for 30 minutes at 37 degree C. H3K9me0 antibody was used to detect reaction products.)
HDAC11, Recombinant Protein (Cat# AAA59743)
Application Data
(HTRF assay for Recombinant HDAC6 activity 1 ?M H3K9ac (1-21 aa) peptide was incubated with different concentrations of HDAC6 protein in a 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 1 mM MgCl2, 2.7 mM KCl, 0.1% BSA for 30 min at 37 ?, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection.)
WB (Western Blot)
(Recombinant NSD2 (E1099K) activity assay.2 ug recombinant nucleosomes was incubated with 0 ug (-), 0.25 ug (+), 0.5 ug (++), 1 ug NSD2 (E1099K)(+++), respectively, in reaction buffer for 3 hours at room temperature. 2 ug histone octamer was used as a control substrate for NSD2 (E1099K) assay. Production (H3K36me2) was detected by Western blot.)
NSD2, Recombinant Protein (Cat# AAA59745)
Application Data
(HTRF assay for HDAC8 protein, His-Tag 3 uM Histone H3K9ac (1-21aa) peptide was incubated with HDAC8 protein, His-Tag in reaction buffer for 30 min at 37 degree C, Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.)
HDAC8, Recombinant Protein (Cat# AAA59754)
Application Data
(HTRF assay for YTHDF2 activity 3 uM oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDF2 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul of DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(Recombinant Mononucleosomes (H2A.Z) - biotinylated, DNA gel. Biotinylated mononucleosomes (H2A.Z) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: free 601 DNA. Lane 3: Intact mononucleosomes. Intact mononucleosomes migrate much higher than free 601 DNA. The agarose gel result shows that almost all of 601 DNA wraps histone octamers to form mononucleosomes. Nucleosomes = Mononucleosomes.)
Mononucleosomes, Recombinant Protein (Cat# AAA59762)
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression:The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification:After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use.
The result is a purified recombinant protein — a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Advantages Of Our Recombinant Proteins:
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency — two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.