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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
At AAA Biotech, also known as AAA Bio or AAABio, we provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Whether you're conducting research on cell expansion, polarization, differentiation, or cell processing, we can offer the exact recombinant proteins you need in order to support your work.
Viewing 1550-1600 of 14163 product results
WB (Western Blot)
(Western blot for recombinant CREBBP (1075-1873) protein activity 0.5 ?g Histone H3 was incubated with 0 ?g (-), 0.5 ?g (+) CREBBP (1075-1873) in 20 ?l reaction system for 2 hours at room temperature. Half of each reaction was run on a 13% SDS-PAGE gel, and products were detected by Western blot. H3ac (pan-acetyl) antibody was used to recognize acetylated histone H3.)
CREBBP, Recombinant Protein (Cat# AAA59768)
Application Data
(ESI-TOF Mass Spec analysis for Recombinant Histone H3K4me3 (MLA). Expected mass: 15285 Da Observed mass: 15285 Da)
Histone H3K4me3, Recombinant Protein (Cat# AAA59777)
Application Data
(ESI-TOF Mass Spec analysis for Recombinant Histone H3K4me1 (MLA). Expected mass: 15257 Da Observed mass: 15257 Da)
Histone H3K4me1, Recombinant Protein (Cat# AAA59789)
SDS-PAGE
(Tau Proteins: Representation of seven Tau proteins, from top to bottom: Tau-441, Tau-410, Tau-412, Tau-381, Tau-383, Tau-352 and Tau-316 (Tau Protein Marker; .)
Tau-441, BRSK1-phosphorylated, Recombinant Protein (Cat# AAA74012)
SDS-PAGE
(Tau Proteins: Representation of seven Tau proteins, from top to bottom: Tau-441, Tau-410, Tau-412, Tau-381, Tau-383, Tau-352 and Tau-316 (Tau Protein Marker; .)
Tau-441, Recombinant Protein (Cat# AAA74027)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
TNNI3K, Recombinant Protein (Cat# AAA74060)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
BRSK1, Recombinant Protein (Cat# AAA73280)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
VacA, Recombinant Protein (Cat# AAA197065)
Ligand affinity chromatography
Application Data
DNMT3L, Recombinant Protein (Cat# AAA72979)
Application Data
SPHK1, Recombinant Protein (Cat# AAA73127)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet)
COVID 19 Spike S1 (K417N, E484K, N501Y, D614G) Coronavirus, Recombinant Protein (Cat# AAA73332)
Bioactivity
(Activity: Sample activity data. For lot specific data please refer to the specific datasheet.)
COVID 19 Spike RBD (R346K, E484K, N501Y) Coronavirus, Recombinant Protein (Cat# AAA73342)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
IRAK4 (catalytic domain), Recombinant Protein (Cat# AAA73620)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PTPRE, Recombinant Protein (Cat# AAA73850)
SDS-PAGE
(Tau Proteins: Representation of seven Tau proteins, from top to bottom: Tau-441, Tau-410, Tau-412, Tau-381, Tau-383, Tau-352 and Tau-316 (Tau Protein Marker; .)
Tau-441, PKA-phosphorylated, Recombinant Protein (Cat# AAA74011)
SDS-PAGE
(Tau Proteins: Representation of seven Tau proteins, from top to bottom: Tau-441, Tau-410, Tau-412, Tau-381, Tau-383, Tau-352 and Tau-316 (Tau Protein Marker; .)
Tau-441, TTBK1-phosphorylated, Recombinant Protein (Cat# AAA74013)
SDS-PAGE
(Tau Proteins: Representation of seven Tau proteins, from top to bottom: Tau-441, Tau-410, Tau-412, Tau-381, Tau-383, Tau-352 and Tau-316 (Tau Protein Marker; .)
Tau-441 (R406W), Recombinant Protein (Cat# AAA74054)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
EGFR (T790M L858R), Recombinant Protein (Cat# AAA73441)
SDS-PAGE
(Streptavidin pull down assay for Recombinant Mononucleosomes (H1.2) - biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1 hr at 4 degree C. Then beads were added to 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.5 ul samples were loaded and run on a 12.5% SDS-PAGE gel and stained by Coomassie Blue. * indicates streptavidin. The SDS-PAGE gel result showed that about 60% of biotinylated mononucleosomes were pulled down by streptavidin beads.)
Mononucleosomes, Recombinant Protein (Cat# AAA60200)
Application Data
(HTRF assay for MER (528-999) activity 1 uM TK substrate was incubated with different concentrations of JAK1 (866-1154) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM MnCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
MER, Recombinant Protein (Cat# AAA60246)
Application Data
(HTRF assay for HDAC3 / NCOR2 complex activity 3 uM Histone H3K9ac (1-21aa) peptide was incubated with different concentrations of HDAC3 / NCOR2 complex in a reaction system with 25 mM Tris-HCl pH 8, 137 mM NaCl, 2.7 mM KCl, 1mM MgCl2 and 0.1 mg/ml BSA for 30 min at 37 degree C, then H3K9me0 antibody and SA-XL665 mixture was added to each for 30 min. Operations were performed at room temp. HTRF assay was used for detection.)
HDAC3/NCOR2, Recombinant Protein (Cat# AAA59734)
Application Data
(HTRF assay for recombinant BRDT (21-137) protein, GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRDT (21-137) protein, GST-tag in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
BRDT, Recombinant Protein (Cat# AAA60165)
Application Data
(Recombinant BRD9 (130-259) activity assay. 3 uM histone peptide H4K5/8/12/16 (ac4) was incubated with BRD7 (130-259) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
BRD9, Recombinant Protein (Cat# AAA59650)
Application Data
(MALDI-TOF for LSD1 / KDM1A protein activity 3 uM H3K4me2 (1-27aa) peptide was incubated with 5 nM and 15 nM of LSD1 / KDM1A protein in reaction buffer containing 50 mM Tris-HCl pH 8.0, 50 nM FAD for 1 hr at room temperature. Single H3K4me2 peptide was used as negative control. MALDI-TOF was used for detection.)
LSD1/KDM1A, Recombinant Protein (Cat# AAA59676)
Application Data
(JMJD2C / KDM4C activity assay.1 uM H3K9me3 peptide was incubated with different concentrations of JMJD2C / KDM4C protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate, 25 uM (NH4)2Fe(SO4)2·6H2O for 1 hour, then 10 ul H3K9me2 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
JMJD2C/KDM4C, Recombinant Protein (Cat# AAA59695)
SDS-PAGE
(Purity: Sample purity data. for lot-specific data, please refer to the specific datasheet.)
COVID 19 Spike RBD (K417T) Coronavirus, Recombinant Protein (Cat# AAA73350)
SDS-PAGE
(Purity : Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
ALK2 (G328W), Recombinant Protein (Cat# AAA73209)
SDS-PAGE
IL31, Recombinant Protein (Cat# AAA48933)
SDS-PAGE
(Tau Proteins: Representation of seven Tau proteins, from top to bottom: Tau-441, Tau-410, Tau-412, Tau-381, Tau-383, Tau-352 and Tau-316 (Tau Protein Marker; .)
Tau-441, Biotinylated, Recombinant Protein (Cat# AAA74025)
SDS-PAGE
(Tau Proteins: Representation of seven Tau proteins, from top to bottom: Tau-441, Tau-410, Tau-412, Tau-381, Tau-383, Tau-352 and Tau-316 (Tau Protein Marker; .)
Tau-441, Recombinant Protein (Cat# AAA74026)
SDS-PAGE
(Tau Proteins: Representation of seven Tau proteins, from top to bottom: Tau-441, Tau-410, Tau-412, Tau-381, Tau-383, Tau-352 and Tau-316 (Tau Protein Marker; .)
Tau-441, Recombinant Protein (Cat# AAA74033)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
MEKK3, Recombinant Protein (Cat# AAA73656)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
NUAK2 (K81R), Recombinant Protein (Cat# AAA73776)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PKAc alpha, Recombinant Protein (Cat# AAA73846)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
PDHK2, Recombinant Protein (Cat# AAA73855)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
EGFR (T790M), Recombinant Protein (Cat# AAA73443)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
EGFR (K716A T790M C797S L858R), Recombinant Protein (Cat# AAA73459)
Application Data
(ESI-TOF Mass Spec analysis for Recombinant Histone H3K9me3 (MLA). Expected mass: 15285 Da Observed mass: 15285 Da)
Histone H3K9me3, Recombinant Protein (Cat# AAA59778)
Application Data
DNMT3A, Recombinant Protein (Cat# AAA72980)
Application Data
SMYD2, Recombinant Protein (Cat# AAA73132)
Application Data
TOPK, Recombinant Protein (Cat# AAA73153)
Application Data
(HTRF assay for Recombinant PHF8 protein activity. 1 uM H3K4me3K9me1 peptide was incubated with PHF8 / KDM7B protein in reaction buffer for 1 hour at room temperature. Anti-H3K9me0 antibody was used to detect reaction product.)
PHF8, Recombinant Protein (Cat# AAA59682)
Application Data
(HTRF Assay for Recombinant BRD2 (344-455) activity. 3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRD2 (344-455) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
BRD2, Recombinant Protein (Cat# AAA59646)
SDS-PAGE
(The purity was determined to be >80% by densitometry.Approx. MW 95kDa)
LATS2, Recombinant Protein (Cat# AAA72892)
WB (Western Blot)
(Western Blot analysis for Mononucleosomes H3K4me3 (EPL) - biotinylated. Unmodified mononucleosomes (Lane 1) and Mononucleosomes H3K4me3 (Lane 2) were detected with anti-H3K4me3 antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Mononucleosomes H3K4me3 can be detected by anti- H3K4me3 antibody. Nucleosomes = Mononucleosomes)
Mononucleosomes H3K4me3, Recombinant Protein (Cat# AAA59763)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
UBE2L6 (UBCH8), Recombinant Protein (Cat# AAA74091)
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression:The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification:After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use.
The result is a purified recombinant protein — a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Advantages Of Our Recombinant Proteins:
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency — two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.