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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
At AAA Biotech, also known as AAA Bio or AAABio, we provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Whether you're conducting research on cell expansion, polarization, differentiation, or cell processing, we can offer the exact recombinant proteins you need in order to support your work.
Viewing 1400-1450 of 10000 product results
Application Data
(Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein SPR DataBiotinylated Human Fc gamma RIIIB (NA2) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 4.3?M as determined in SPR assay (Biacore T200).)
Fc gamma RIIIB/CD16b (NA2), Recombinant Protein (Cat# AAA76162)
ELISA
(Biotinylated Human IL-13 Protein ELISA DataImmobilized Biotinylated Human IL-13, His Tag at 2?g/ml (100?l/well) on the streptavidin (5?g/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 1.4?g/ml determined by ELISA.)
IL-13, Recombinant Protein (Cat# AAA76178)
Papilloma Virus (HPV-16), Recombinant Protein (Cat# AAA77447)
Purified
HEPATITIS B SURFACE ANTIGEN AD, Recombinant Protein (Cat# AAA50541)
SDS-PAGE
(Figure. SDS-PAGE)
Neuronal Pentraxin II (NPTX2), Recombinant Protein (Cat# AAA150667)
SDS-PAGE
(SDS-PAGE)
Erythropoietin Receptor (EPOR), Recombinant Protein (Cat# AAA150994)
SDS-PAGE
(SDS-PAGE)
Transforming Growth Factor Beta 1 (TGFb1), Recombinant Protein (Cat# AAA151002)
SDS-PAGE
(SDS-PAGE)
Paraoxonase 1 (PON1), Recombinant Protein (Cat# AAA152190)
Amino Acid Info
(Amino Acid info)
Proprotein Convertase Subtilisin/Kexin Type 6 (PCSK6), Recombinant Protein (Cat# AAA153601)
Amino Acid Info
Tankyrase 1 Binding Protein 1 (TNKS1BP1), Recombinant Protein (Cat# AAA153642)
Amino Acid Info
Retinoic Acid Receptor Responder 1 (RARRES1), Recombinant Protein (Cat# AAA153667)
Amino Acid Info
(Amino Acid info)
Collagen Type XIX (COL19), Recombinant Protein (Cat# AAA153516)
Amino Acid Info
Fibroblast Activation Protein Alpha (FAPa), Recombinant Protein (Cat# AAA153533)
Amino Acid Info
(Amino Acid info)
Kruppel Like Factor 2, Lung (KLF2), Recombinant Protein (Cat# AAA153583)
Amino Acid Info
(Amino Acid info)
Aquaporin 4 (AQP4), Recombinant Protein (Cat# AAA153360)
Amino Acid Info
Immunoglobulin G1 (IgG1), Recombinant Protein (Cat# AAA153261)
Application Data
Collagen Type I Alpha 1 (COL1a1), Recombinant Protein (Cat# AAA134217)
SDS-PAGE
Apolipoprotein E (ApoE), Recombinant Protein (Cat# AAA55951)
SDS-PAGE
Peptidylprolyl isomerase A (PPIA), Recombinant Protein (Cat# AAA55958)
SDS-PAGE
ALDH1B1, Recombinant Protein (Cat# AAA55966)
Application Data
(SDS-PAGE)
MMP9, Recombinant Protein (Cat# AAA55970)
SDS-PAGE
Membrane cofactor protein (CD46), Recombinant Protein (Cat# AAA55976)
COVID 19 Spike Protein, N-terminal Domain (NTD) S1 Coronavirus, Recombinant Protein (Cat# AAA58066)
HRP-II, Recombinant Protein (Cat# AAA58074)
SDS-PAGE
Serum Amyloid A1, Recombinant Protein (Cat# AAA60566)
Application Data
(Figure 5: ATAC-Seq Results are Consistent with Pre-indexed Assembled Tn5 Transposomes and Standard Tn5 Transposomes. 50,000 fresh K562 cells were assayed in ATAC-Seq with either Active Motif’s Pre-indexed Assembled Tn5 Transposomes (top four tracks) or standard, non-indexed Tn5 Assembled Transposomes (bottom track). ATAC-Seq results were consistent between both methods. Browser tracks are shown at Chr6:159,668,373-159,821,466.)
WB (Western Blot)
(Western blot assay for Recombinant Mononucleosomes H3.1 (K27M) - biotin 2 ug Recombinant Mononucleosomes H3.1 (K27M) - biotin were incubated with 0 or 100 nM PRC2 respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 3 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H3K27me1 antibody . Mononucleosomes H3.1 were used as a control. The Western Blot result shows that only the H3K27 of mononucleosomes H3.1 can be methylated by PRC2.)
Mononucleosomes H3.1, Recombinant Protein (Cat# AAA60193)
SDS-PAGE
(Streptavidin pull down assay for Recombinant Mononucleosomes H3.2 – biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1hr at 4 degree C. Streptavidin beads were washed 3 times with 1 ml binding buffer. Then the beads were added 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.4 ul samples were loaded and run on a 12.5% SDS-PAGE gel and stained by Commassie blue. The SDS-PAGE gel result showed that almost all of biotinylated mononucleosomes were pulled down by streptavidin beads.)
Mononucleosomes H3.2, Recombinant Protein (Cat# AAA60216)
SDS-PAGE
(Streptavidin pull down assay for Recombinant Mononucleosomes H3.3 (K9I) – biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1 hr at 4 degree C. Streptavidin beads were washed 3 times with 1 ml binding buffer. The beads were added to 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.4 ul samples were loaded and run on a 12.5% SDS-PAGE gel stained with Coomassie blue. The SDS-PAGE gel shows that almost all of the biotinylated mononucleosomes were pulled down by streptavidin beads.)
Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60220)
SDS-PAGE
Glycoprotein of Rabies Virus, Recombinant Protein (Cat# AAA62082)
SDS-PAGE
(SDS-PAGE: purified recombinant E protein of SARS-CoV-2.)
COVID 19 Envelope Protein (E) Coronavirus, Recombinant Protein (Cat# AAA62091)
SDS-PAGE
(SDS-PAGE: purified HA1 (A/California/07/2009)(H1N1) protein; A, reducing condition; B, non-reducing condition.)
HA1(A/California/07/2009)(H1N1), Recombinant Protein (Cat# AAA62206)
SDS-PAGE
(SDS-PAGE: purified HA (Y98F)(aa 18-530)(A/Brisbane/02/2018/H1N1) protein)
HA, Recombinant Protein (Cat# AAA62218)
TSHR, Recombinant Protein (Cat# AAA59126)
COVID 19 Spike RBD Coronavirus, Recombinant Protein (Cat# AAA59153)
Clostridium difficile Toxin A, Recombinant Protein (Cat# AAA58294)
Clostridium difficile Toxin B, Recombinant Protein (Cat# AAA58295)
pro-Brain Natriuretic Peptide, N-terminal (NT-proBNP) (a.a. 1-76) Recombinant, Recombinant Protein (Cat# AAA58298)
Rubella Virus E2 protein, Recombinant, Recombinant Protein (Cat# AAA58305)
Herpes Simplex Virus-2 (HSV-2) Glycoprotein G, Recombinant, Recombinant Protein (Cat# AAA58307)
Toxoplasma gondii p30 (SAG1) Recombinant Protein, Recombinant Protein (Cat# AAA58311)
GS-4B Sepharose-Affinity Purification
West Nile Virus Envelope Protein, N-terminal, Recombinant, Recombinant Protein (Cat# AAA58321)
Borrelia burgdorferi (Lyme) Osp-A, Recombinant, Recombinant Protein (Cat# AAA58325)
Hepatitis C Virus (HCV) Core Antigen (a.a. 1-120) 16kDa Recombinant, Recombinant Protein (Cat# AAA58333)
Herpes Simplex Virus-1 (HSV-1) Glycoprotein D Glycosylated, Recombinant, Recombinant Protein (Cat# AAA58334)
NGAL, Recombinant Protein (Cat# AAA58394)
SDS-PAGE
Mitochondrial Glycerol-3-Phosphate Dehydrogenase (mGPD), Recombinant Protein (Cat# AAA58754)
WB (Western Blot)
(Histone methyltransferase activity assay comparing recombinant polynucleosomes and histone octamers as substrates. 2 ug Recombinant Polynucleosomes H3.3 (G34R) were incubated with DOT1L in reaction buffer for 3 hours at room temperature. Western Blot was used for detecting the generation of reaction products (H3K79me1). DOT1L only and polynucleosomes only were used as negative control. The Western Blot result shows that polynucleosomes are more suitable substrate for DOT1L than histone octamers.)
Polynucleosomes H3.3, Recombinant Protein (Cat# AAA59749)
SDS-PAGE
(SDS-PAGE (4-20%): purified recombinant E7 protein of HPV 16)
E7 Protein of Human Papillomavirus (HPV) 16, Recombinant Protein (Cat# AAA62268)
Application Data
HIV-1 gp120, Recombinant Protein (Cat# AAA62182)
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression:The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification:After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use.
The result is a purified recombinant protein — a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Advantages Of Our Recombinant Proteins:
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency — two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.