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▼Recombinant Proteins
Recombinant proteins are purified laboratory reagents created through genetic engineering for advanced biomedical research, such as immunology, neuroscience, cancer research, and more.
At AAA Biotech, also known as AAA Bio or AAABio, we provide high-quality recombinant proteins. Our various protein products are carefully tested for consistent and reliable performance. With our proteins, we offer stringent quality control, lot-to-lot consistency, and precise specificity to help you achieve accurate results in your research.
Whether you're conducting research on cell expansion, polarization, differentiation, or cell processing, we can offer the exact recombinant proteins you need in order to support your work.
Viewing 1600-1650 of 10000 product results
ELISA
(Biotinylated Human FGFR3 alpha (IIIc) Protein ELISA DataImmobilized Biotinylated Human FGFR3 alpha (IIIc) at 0.5?g/ml (100?l/Well) on the plate. Dose response curve for Anti-FGFR3 Antibody, hFc Tag with the EC50 of 9.7ng/ml determined by ELISA.)
FGFR3 alpha (IIIc), Recombinant Protein (Cat# AAA76262)
ELISA
(Biotinylated Human VEGF R1/FLT-1 Protein ELISA DataImmobilized Human PGF, hFc Tag at 0.5?g/ml (100?l/Well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 48ng/ml determined by ELISA.)
VEGF R1/FLT-1, Recombinant Protein (Cat# AAA76266)
ELISA
(Biotinylated Human IGF1R/CD221 Protein ELISA DataImmobilized Anti-IGF1R Antibody at 0.5?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human IGF1R, His Tag with the EC50 of 48.0ng/ml determined by ELISA.)
IGF1R/CD221, Recombinant Protein (Cat# AAA76148)
ELISA
(Biotinylated Human CLEC12A/MICL/CLL-1 Protein ELISA DataImmobilized Anti-CLEC12A Antibody, hFc Tag at 0.5?g/ml (100?l/Well) on the plate. Dose response curve for Biotinylated Human CLEC12A, hFc Tag with the EC50 of 30.7ng/ml determined by ELISA.)
CLEC12A/MICL/CLL-1, Recombinant Protein (Cat# AAA76175)
Application Data
(Biotinylated Human LAIR2/CD306 Protein SPR DataBiotinylated Human LAIR2, His-Avi Tag immobilized on CM5 Chip can bind Native Human Collagen I, No Tag with an affinity constant of 1.80 nM as determined in SPR assay (Biacore T200).)
LAIR2/CD306, Recombinant Protein (Cat# AAA76179)
IA (Immunoassay)
(Coated AAA78012: 3.2ug , 0.64ug , 0.128ug , 32ngMouse anti-Den NS1: 0.774 , 0.766 , 0.324 , 0.057Control mouse serum: 0.186 , 0.153 , 0.058 , 0.023S/BKG: 4.16 , 5.0 , 5.58 , 2.47Polyclonal mouse anti-dengue NS 1, 1: 1 00Control mouse serum: 1: 100)
Dengue 3 full length NS1, Recombinant Protein (Cat# AAA78012)
SDS-PAGE
HPV 18, Recombinant Protein (Cat# AAA78024)
WB (Western Blot)
(Figure 2: Western blot analysis of Trim29: Anti- Trim29 antibody (Clone: ABM43D2) was tested at 2ug/ml on purified recombinant Trim29 protein expressed in HEK 293 cells .)
TRIM29, Recombinant Protein (Cat# AAA78599)
IL-6, Recombinant Protein (Cat# AAA50530)
SDS-PAGE
(SDS-PAGE)
Alpha And Gamma-Adaptin Binding Protein (aAgAB), Recombinant Protein (Cat# AAA151091)
SDS-PAGE
(SDS-PAGE)
Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g), Recombinant Protein (Cat# AAA151092)
SDS-PAGE
(Figure. SDS-PAGE)
Actin Alpha 2, Smooth Muscle (ACTa2), Recombinant Protein (Cat# AAA150687)
Amino Acid Info
Calnexin (CNX), Recombinant Protein (Cat# AAA153324)
Amino Acid Info
(Amino Acid info)
Fibronectin Type III Domain Containing Protein 5 (FNDC5), Recombinant Protein (Cat# AAA153719)
Amino Acid Info
(Amino Acid info)
Rhodopsin (RHO), Recombinant Protein (Cat# AAA153547)
SALL4, Recombinant Protein (Cat# AAA55964)
SDS-PAGE
Cytochrome P450 3A (CYP3A), Recombinant Protein (Cat# AAA55979)
Application Data
(MALDI-TOF for PHD2 (EGLN1) protein activity 3 uM HIF1A (HIF-1a) peptide (peptide sequence: biotin-DLDLEALAPYIPADDDFQL) was incubated with 300 nM PHD2 (EGLN1) protein in a 30 ul reaction system containing 20 mM Tris-HCl pH 7.5, 5 mM KCl, 1.5 mM MgCl2, 1 mM DTT, 100 uM 2-oxoglutarate, 100 uM ascorbate and 50 uM (NH4)2Fe(SO4)2·6H2O for 2 hr at 3°ree;. Single 3 uM HIF1A peptide was used as negative control. MALDI-TOF was used for detection.)
PHD2, Recombinant Protein (Cat# AAA60116)
WB (Western Blot)
(Western blot assay for Recombinant Mononucleosomes H3.1 (K4M) 2 ug Recombinant Mononucleosomes H3.1 (K4M) werenincubated with 0 or 10 nM MLL2 Complex respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 ?M SAM for 3 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H3K4me2 antibody and H4 antibody , respectively. H4 was detected as loading control. Recombinant Mononucleosomes H3.1 was used as a positive control. The result shows that no H3K4me2 products were generated when Mononucleosomes H3.1 (K4M) were incubated with MLL2 Complex.)
Mononucleosomes H3.1, Recombinant Protein (Cat# AAA60183)
SDS-PAGE
(Recombinant Mononucleosomes H3.1 (R8G) 12.5% SDS-PAGE gel stained with Coomassie Blue. MW: 108 kDa Purity: >95%)
Mononucleosomes H3.1, Recombinant Protein (Cat# AAA60189)
SDS-PAGE
(Streptavidin pull down assay for Recombinant Mononucleosomes H3.1 (K9M) - biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1 hr at 4 degree C. Streptavidin beads were washed 3 times with 1 ml binding buffer. Then the beads were added to 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.4 ul samples were loaded and run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. * indicates streptavidin. The SDS-PAGE gel result shows that >90% of biotinylated mononucleosomes were pulled down by streptavidin beads.)
Mononucleosomes H3.1, Recombinant Protein (Cat# AAA60192)
SDS-PAGE
(Streptavidin pull down assay for Recombinant Mononucleosomes H3.3 (K18M) - biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1 hr at 4 degree C. Streptavidin beads were washed 3 times with 1 ml binding buffer. Then the beads were added 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.4 ul samples were loaded and run on a 12.5% SDS-PAGE gel and stained by Coomassie blue. * indicates streptavidin. The SDS-PAGE gel result shows that almost all of biotinylated mononucleosomes were pulled down by streptavidin beads.)
Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60208)
Application Data
(Recombinant Mononucleosomes H3.3 (K4M) DNA gel Recombinant Mononucleosomes H3.3 (K4M) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA which was used for assembly of nucleosome. Lane 3: Intact mononucleosomes H3.3 (K4M). Intact mononucleosomes H3.3 (K4M) migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrapped histone octamers to form nucleosomes.)
Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60228)
Application Data
(Recombinant DmTet (1634-1986, 2601-2708) activity assay 500 nM of 30 nt ssDNA oligos with a 5mCpG site were incubated with 0 ug, 0.625 ug, 1.25 ug, 2.5 ug or 5 ug DmTet (1634-1986 and 2601-2708), respectively, in a 20 ul reaction system at 34 degree C for 1 hour. Sample was concentrated to 10 ul, and 1 ul was spotted onto nylon membrane. Anti-5hmC Ab and anti-5CaC Ab were used to detect products. DmTet (1634-1986 and 2601-2708) activity assay was detected by Dot-Blot.)
DmTet, Recombinant Protein (Cat# AAA60247)
Chikungunya E1 Wild-Type, Recombinant Protein (Cat# AAA58046)
Epstein-Barr Virus (EBV) Epstein-Barr Virus Nuclear Antigen-1 (EBNA-1), Recombinant Protein (Cat# AAA58049)
Hepatitis C Virus (HCV) Core Antigen (a.a. 2-120) genotype 3, Recomb., Recombinant Protein (Cat# AAA58303)
HTLV-I p24 Recombinant, Recombinant Protein (Cat# AAA58304)
Organic extraction and S-300 and HPLC
Cytomegalovirus (CMV) pp65 (UL83), (AD169 Strain), Recombinant, Recombinant Protein (Cat# AAA58309)
Herpes Simplex Virus-1 (HSV-1) Glycoprotein D, Recombinant, Recombinant Protein (Cat# AAA58312)
Herpes Simplex Virus-2 (HSV-2) Glycoprotein D, Recombinant, Recombinant Protein (Cat# AAA58314)
Hepatitis B Surface Antigen (HBsAg) (adw) Recombinant, Recombinant Protein (Cat# AAA58319)
Purification is conducted from broken yeast cells by the following scheme: clarification, microfiltration, ultrafiltration, adsorption chromatography, ion-exchange chromatography, ultracentrifugation and sterile filtration.
Hepatitis B Core Antigen (HBcAg) (a.a. 1-183) 18kDa Recombinant, Recombinant Protein (Cat# AAA58323)
Hepatitis B Core Antigen (HBcAg) (183 a.a.) Recombinant, Recombinant Protein (Cat# AAA58331)
Testing Results: Pass
>=95% Nucleocapsid, protein as a single peak, performed by SEC-HPLC.
Testing Results: Pass
Immunospecifity verified by Western Blot
Herpes Simplex Virus-2 (HSV-2) Glycoprotein G, Recombinant, Recombinant Protein (Cat# AAA58335)
Procalcitonin, Recombinant Protein (Cat# AAA58347)
Dengue Virus Type 2, EP, Recombinant Protein (Cat# AAA58406)
NaN3
SDS-PAGE
(Purified glycoprotein H (EBV) protein (aa1-679))
EBV (HHV-4) Glycoprotein H (gH), Recombinant Protein (Cat# AAA62083)
Application Data
(E: purified hCD317 (deltaTMs))
His tagged ectodomain, Recombinant Protein (Cat# AAA62174)
Application Data
HIV-1 gp120, Recombinant Protein (Cat# AAA62177)
Application Data
HIV-1 gp120, Recombinant Protein (Cat# AAA62179)
Application Data
CD4 ectodomain, Recombinant Protein (Cat# AAA62185)
SDS-PAGE
(SDS-PAGE: purified HA (A/Victoria/2570/2019)(H1N1)pdm09-like virus (reducing condition))
HA (A/Victoria/2570/2019)(H1N1)-pdm09-like virus, Recombinant Protein (Cat# AAA62230)
SDS-PAGE
(SDS-PAGE: NP (A/Brisbane/59/2007)(H1N1) protein)
NP (A/Brisbane/59/2007) (H1N1), Recombinant Protein (Cat# AAA62234)
Application Data
(NP (A/Brisbane/10/2007)(H3N2) protein)
NP (A/Brisbane/10/2007)(H3N2), Recombinant Protein (Cat# AAA62208)
SDS-PAGE
(The purity was determined to be >90% by desitometry.Approx. MW 65kDa.)
IRS1, Recombinant Protein (Cat# AAA72896)
SDS-PAGE
(The purity was determined to be >85% by densitometry. Approx. MW 84kDa)
MDM2, Recombinant Protein (Cat# AAA72902)
SDS-PAGE
(The purity was determined to be >90% by densitometry. Approx MW 68kDa)
ERK2, Recombinant Protein (Cat# AAA72917)
FCM/FACS (Flow Cytometry)
Calmodulin1, Recombinant Protein (Cat# AAA72926)
Application Data
AMPK, Recombinant Protein (Cat# AAA73079)
What Are Recombinant Proteins?
A recombinant protein is created by inserting the gene of interest into a host system like E. coli, mammalian cells, or insect cells. This protein expression method results in the large-scale production of protein in a controlled environment with highly precise structures and functionality. Proteins produced via the “Recombinant” methodology have proven themselves crucial in various biological research areas, including immunology, neuroscience, cancer research, drug delivery and development, and more.
Core Recombinant Protein Product Lines:
- Human: Produced from human genes (e.g., growth factors, cytokines).
- Animal: Derived from mouse, rat, or other animals (e.g., mouse antibodies, enzymes).
- Bacterial: Produced in bacteria like E. coli (e.g., enzymes, bacterial toxins).
- Yeast: Produced in yeast cells (e.g., surface proteins, metabolic enzymes).
- Insect:Expressed in insect cells (e.g., viral proteins, glycoproteins).
- Viral: Derived from viral genomes (e.g., spike proteins for vaccine development).
How Does It Work?
- Gene Identification and Cloning: Scientists first find the exact gene that codes for the protein they are interested in producing, and then create a copy of said gene.
- Recombinant DNA Creation: The copied gene is then inserted into a vector (a vehicle-like plasmid) that can be transported safely into your intended expression host cell/system.
- Host Cell Transformation: The vector (with the foreign gene inside) is shuttled into a host cell. This host can be a bacterium (like E. coli), yeast, insect cells, or mammalian cells, depending on the type of protein or protein characteristics needed.
- Protein Expression:The host cell “reads” the copied gene that was shuttled into it, and begins to produce the gene’s coded-for protein using its own cellular machinery. The host can continue to multiply, and with each new cell, more protein is produced.
- Protein Purification:After enough protein has been made, scientists collect the host cell and separate out the target protein. The protein is then carefully purified so it is free of contaminants, ideally active, and also safe for use.
The result is a purified recombinant protein — a lab-made protein that is biologically identical (in most respects) to the natural/native version.
We take this process a step further, offering recombinant proteins that are produced with precise control over sequence modifications, expression levels, and large-scale production. This ensures high-quality, consistent results that will be able to support your research needs and empower your scientific discoveries.
Some of the expression hosts/systems used in recombinant protein generation by AAA Biotech include:
- Escherichia coli (E. coli)
- Human (Homo sapiens)
- Chinese Hamster
- Yeast
- Insect cells (e.g., Baculovirus expression system)
Advantages Of Our Recombinant Proteins:
Recombinant Protein Offerings
| S.No | Protein Expression Systems | Quality and Bioactivity | Formats and Flexibility |
|---|---|---|---|
| 01. | E. coli, HEK293, CHO, yeast, and insect cells | Validated high bioactivity and binding activity | Ready-to-use formats (lyophilized or liquid formulations available) |
| 02. | 675+ recombinant protein products | High protein purity (≥95%) | Available in flexible pack sizes |
| 03. | Custom protein production (His-tag, GST, FLAG, and Fc fusion) | Lot-to-lot consistency through QC protocols | Wide range of targets (Cytokines, growth factors, enzymes, receptors, signaling proteins, etc.) |
Applications Of Our Recombinant Proteins
- Studying protein interactions (protein–protein or protein–DNA).
- Testing biological pathways with functional/activity assays.
- Creating standard curves in ELISA and other tests.
- Making antigens/immunogens for antibody production.
- Drug discovery and screening.
- Finding and confirming biomarkers.
- Researching cell signaling and immunity.
- Developing vaccines.
Read more about the applications of the recombinant proteins here!
Why Buy Recombinant Proteins from AAA Biotech?
1. High Purity & Verified Quality:
Most of our proteins are ≥ 95% pure. We also test the biological activity of our products where it's relevant (it will be indicated on the product page).
2. Multiple Expression Systems:
We offer proteins made in multiple host systems, and this enables researchers to pick the system that gives them the best folding, post-translational modifications, or functionality for their experiment requirements.
3. Custom & Flexible Options:
Need a special tag (His, GST, FLAG, Fc fusion)? Or a custom variant? We can provide it. Our proteins come in formats that are lab-friendly: lyophilized (dry) or “ready-to-use” liquid.
4. Rigorous Quality Control & Documentation:
Every batch is backed by strong QC. This means you can rely on product consistency — two different batches will perform similarly.
5. Wide Variety & Global Availability:
We carry a large and growing catalog of recombinant proteins to cover almost all needs in research (immunology, oncology, vaccine work, etc.)
6. Researcher-Focused Support:
Technical support, clear documentation, and user-friendly product pages help you select the right protein for your work. Plus, our user-friendly packaging and handling, and global shipping are all designed to reduce delays, damage, and hassles.
Order Recombinant Proteins Today!
We are committed to supporting the research community with recombinant proteins that display exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
We have a track record of delivering proteins that work — large numbers of researchers rely on them in many published studies. Browse our catalog to find the perfect protein for your research applications now!
FAQ
1. What is involved in the purification of recombinant proteins?
The purification process of these recombinant proteins is performed to isolate the specific protein produced by the host cell from unwanted substances. The process generally includes techniques such as “affinity chromatography” to separate the protein from other proteins and impurities. AAA Biotech generally offers proteins ≥ 95% pure, depending on the protein type.
2. What are some examples of popular AAA Biotech recombinant proteins?
Some examples of recombinant proteins include:
Retinoblastoma Protein (Cat # AAA10852) – reportedly used in vaccine research and antibody development. Hepatitis B Surface Antigen (HBsAg) (Cat# AAA13446) – reportedly used in diagnostic tests and vaccine development for Hepatitis B. High-Mobility Group Box 1 (HMGB1) (Cat# AAA10851) – reportedly used in inflammation and immune response research.
3. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product (only upon request for some products).
4. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
5. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.