NF-kB p65 Recombinant Protein | RELA recombinant protein

NF-kB p65 Protein, Human, Recombinant (aa 1-306, GST Tag)

Cat. Num. AAA258003

• Gene Names: RELA; p65; NFKB3
• Purity: >85% as determined by SDS-PAGE
• Synonyms: NF-kB p65; N/A; NF-kB p65 Protein, Human, Recombinant (aa 1-306, GST Tag); Human RELA/Transcription factor p65/NFkB p65 Protein (aa 1-306, GST Tag); NFKB3 Protein, Human; p65 Protein, Human; v-rel avian reticuloendotheliosis viral oncogene homolog A; RELA recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 150mM NaCl, 10mM GSH, 5% trehalose, 5% mannitol, 0.01% tween-80
• Sequence: Met1-Tyr306

• Source: Human
• Predicted N Terminal: Met
• Tag: GST
• Protein Construction: A DNA sequence encoding the human RELA (Q04206-1) RHD domian (Met 1-Tyr 306) was fused with the GST tag at the N-terminus.
• Reconstitution: It is recommended that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is mesured by UV-Vis.
• Preparation and Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for RELA recombinant protein

Background: RELA (v-rel reticuloendotheliosis viral oncogene homolog A), also known as Nuclear factor NF-kappa-B p65 subunit, or Transcription factor p65, is a transcription factor expressed in growth plate chondrocytes where it facilitates chondrogenesis. The v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA) gene encodes the major component of the NF-B complex. NF-kappaB is a generic name for an evolutionarily conserved transcription-factor system that contributes to the mounting of an effective immune response but is also involved in the regulation of cell proliferation, development, and apoptosis. The implication of NF-kappaB in central biological processes and its extraordinary connectivity to other signaling pathways raise a need for highly controlled regulation of NF-kappaB activity at several levels. The mammalian Rel/NF-kappaB family of transcription factors, including RelA, c-Rel, RelB, NF-kappaB1 (p50 and its precursor p105), and NF-kappaB2 (p52 and its precursor p100), plays a central role in the immune system by regulating several processes ranging from the development and survival of lymphocytes and lymphoid organs to the control of immune responses and malignant transformation.

Product Categories/Family for RELA recombinant protein

Apoptosis Transcription Factors and Regulators; Death Receptor Signaling

References

Hashimoto R, et al. (2011) Variants of the RELA gene are associated with schizophrenia and their startle responses. Neuropsychopharmacology. 36(9): 1921-31.Vallabhapurapu S, et al. (2009) Regulation and function of NF-kappaB transcription factors in the immune system. Annu Rev Immunol. 27: 693-733.Schmitz ML, et al. (2004) NF-kappaB: a multifaceted transcription factor regulated at several levels. Chembiochem. 5(10): 1348-58.

NCBI and Uniprot Product Information

• NCBI GI #: 223468681
• NCBI GeneID: 5970
• NCBI Accession #: NP_001138610.1
• NCBI GenBank Nucleotide #: NM_001145138.1
• UniProt Accession #: Q04206; Q6GTV1; Q6SLK1
• Molecular Weight: Molecular Mass Note: The recombinant human RELA/GST chimera consists of 540 amino acids and has a predicted molecular mass of 62 kDa. It migrates as an approxiamtely 58 kDa band in SDS-PAGE under reducing conditions.
• NCBI Official Full Name: transcription factor p65 isoform 2
• NCBI Official Synonym Full Names: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• NCBI Official Symbol: RELA
• NCBI Official Synonym Symbols: p65; NFKB3
• NCBI Protein Information: transcription factor p65; NF-kappa-B p65delta3; nuclear factor NF-kappa-B p65 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
• UniProt Protein Name: Transcription factor p65
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3
• UniProt Entry Name: TF65_HUMAN