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8519 results for "Protein" - showing 8250-8300


FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A431 cells using anti-HIF-1 alpha/HIF1A antibody (AAA19213).Overlay histogram showing A431 cells stained with AAA19213 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HIF-1 alpha/HIF1A Antibody (AAA19213, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

HIF-1 alpha/HIF1A, Polyclonal Antibody (Cat# AAA19213)

Full Name
Anti-HIF-1 alpha/HIF1A Antibody
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-MERTK antibody (AAA19219).Overlay histogram showing HepG2 cells stained with AAA19219 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MERTK Antibody (AAA19219, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MERTK, Polyclonal Antibody (Cat# AAA19219)

Full Name
Anti-MERTK Antibody
Gene Names
MERTK; MER; RP38; c-mer
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of U937 cells using anti-Coronin 1a/TACO/CORO1A antibody (AAA19291).Overlay histogram showing U937 cells stained with AAA19291 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Coronin 1a/TACO/CORO1A Antibody (AAA19291, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Coronin 1a/TACO/CORO1A, Polyclonal Antibody (Cat# AAA19291)

Full Name
Anti-Coronin 1a/TACO/CORO1A Antibody
Gene Names
CORO1A; p57; TACO; CLABP; HCORO1; CLIPINA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence) (Figure 7. IF analysis of METTL1 using anti- METTL1 antibody (AAA19336).METTL1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-METTL1 Antibody (AAA19336) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

METTL1, Polyclonal Antibody (Cat# AAA19336)

Full Name
Anti-METTL1 Antibody
Gene Names
METTL1; TRM8; TRMT8; C12orf1; YDL201w
Reactivity
Human, Rat
Applications
WB, IHC-P, ICC, IF, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HEPA1-6 cells using anti-Rad9b antibody (AAA19340).Overlay histogram showing HEPA1-6 cells stained with AAA19340 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rad9b Antibody (AAA19340, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Rad9b, Polyclonal Antibody (Cat# AAA19340)

Full Name
Anti-Rad9b Antibody
Gene Names
Rad9b; BC021784; A630082N15Rik
Reactivity
Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence) (Figure 6. IF analysis of GRSF1 using anti- GRSF1 antibody (AAA19315).GRSF1 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- GRSF1 Antibody (AAA19315) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

GRSF1, Polyclonal Antibody (Cat# AAA19315)

Full Name
Anti-GRSF1 Antibody
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of THP-1 cells using anti-PRMT8 antibody (AAA19321).Overlay histogram showing THP-1 cells stained with AAA19321 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRMT8 Antibody (AAA19321,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PRMT8, Polyclonal Antibody (Cat# AAA19321)

Full Name
Anti-PRMT8 Antibody
Gene Names
PRMT8; HRMT1L3; HRMT1L4
Reactivity
Human, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence) (Figure 6. IF analysis of CD72 using anti- CD72 antibody (AAA19329).CD72 was detected in immunocytochemical section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- CD72 Antibody (AAA19329) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

CD72, Polyclonal Antibody (Cat# AAA19329)

Full Name
Anti-CD72 Antibody
Gene Names
Cd72; CD72c; Ly-19; Ly-32; Lyb-2; Ly-m19
Reactivity
Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-DDX1 antibody (AAA19379).Overlay histogram showing MCF-7 cells stained with AAA19379 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- DDX1 Antibody (AAA19379, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

DDX1, Monoclonal Antibody (Cat# AAA19379)

Full Name
Anti-DDX1 Antibody (monoclonal, 2D4)
Gene Names
DDX1; DBP-RB; UKVH5d
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)

CD25, Monoclonal Antibody (Cat# AAA12044)

Full Name
MOUSE ANTI RAT CD25:RPE
Gene Names
Il2ra; IL2RAC
Applications
FC/FACS
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human prostate cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Calsenilin/KCNIP3, Polyclonal Antibody (Cat# AAA31279)

Full Name
Phospho-Calsenilin/KCNIP3 (Ser63) Antibody
Gene Names
KCNIP3; CSEN; DREAM; KCHIP3
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

SRPK2, Polyclonal Antibody (Cat# AAA31293)

Full Name
Phospho-SRPK2 (Ser494/Ser497) Antibody
Gene Names
SRPK2; SFRSK2
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

MSH2, Polyclonal Antibody (Cat# AAA31299)

Full Name
Phospho-MSH2 (Tyr238) Antibody
Gene Names
MSH2; FCC1; COCA1; HNPCC; LCFS2; HNPCC1
Reactivity
Human, Mouse, Rat
Applications
IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry) (Overlay Peak curve showing Hela cells stained with AAA28066 (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1?g/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1?g/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)

ACTB, Monoclonal Antibody (Cat# AAA28066)

Full Name
ACTB Monoclonal Antibody
Reactivity
Human, Mouse, Rat, Rabbit
Applications
EIA, WB, IHC, IF, FC/FACS, IP
Purity
>95%, Protein A purified
Pricing
IP (Immunoprecipitation) (Immunoprecipitating GAPDH in Hela whole cell lysate Lane 1: Mouse control IgG instead of AAA27043 in Hela whole cell lysate. Lane 2: AAA27043 (5ul) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (10ug) For western blotting, the blot was detected with AAA27043 at 1:5000, and a HRPconjugated Protein G antibody was used as the secondary antibody at 1:2000)

GAPDH, Monoclonal Antibody (Cat# AAA27043)

Full Name
GAPDH Monoclonal Antibody
Reactivity
Human, Rat, Rabbit, Mouse
Applications
EIA, WB, IHC, IP, IF
Purity
>95%, Protein G purified
Pricing
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

F4/80, Monoclonal Antibody (Cat# AAA12164)

Full Name
RAT ANTI MOUSE F4/80:Biotin
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
FC/FACS
Pricing
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

F4/80, Monoclonal Antibody (Cat# AAA12166)

Full Name
RAT ANTI MOUSE F4/80:FITC
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
FC/FACS, IF
Pricing
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

F4/80, Monoclonal Antibody (Cat# AAA12168)

Full Name
RAT ANTI MOUSE F4/80:FITC
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
FC/FACS, IF
Pricing
Application Data (Analysis of Protein Array containing more than 19, 000 full-length human proteins using PU.1 Mouse Monoclonal Antibody (PU1/2446). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

PU.1 (SPI-1), Monoclonal Antibody (Cat# AAA23903)

Full Name
PU.1 (SPI-1) (B-Cell Marker)
Gene Names
SPI1; OF; PU.1; SFPI1; SPI-1; SPI-A
Reactivity
Human. Others not known.
Applications
WB, IHC
Pricing
Application Data (Analysis of Protein Array containing more than 19, 000 full-length human proteins using CD10 Mouse Monoclonal Antibody (MME/1870)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

CD10, Monoclonal Antibody (Cat# AAA23901)

Full Name
CD10 (Membrane Metalloendopeptidase)
Gene Names
MME; NEP; SFE; CD10; CALLA; CMT2T; SCA43
Reactivity
Human. Others not tested.
Applications
IHC
Pricing
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using HER-2 Mouse Monoclonal Antibody (ERBB2/3080). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

HER-2/c-erbB-2/neu/CD340, Monoclonal Antibody (Cat# AAA23912)

Full Name
HER-2/c-erbB-2/neu/CD340
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human
Applications
IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

HP1 alpha, Polyclonal Antibody (Cat# AAA31332)

Full Name
Phospho-HP1 alpha (Ser92) Antibody
Gene Names
CBX5; HP1; HP1A
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

MCM2, Polyclonal Antibody (Cat# AAA31329)

Full Name
Phospho-MCM2 (Ser41) Antibody
Gene Names
MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (Published clone specific image: Flow cytometric analysis of AM from NO2-exposed and control rats. Rats were exposed to NO2 for the indicated times and BAL cells were stained with antibodies to ED7, ED9, RM-4, and OX-6. To overcome autofluorescence signals, primary antibodies were detected using a biotin-PE/streptavidin-anti-streptavidin enhancing system and labeling of AM was analyzed by flow cytometry following gating by help of forward and sideward scatter properties. Shown are representative results of at least six animals per group.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)

CD172a, Monoclonal Antibody (Cat# AAA11888)

Full Name
MOUSE ANTI RAT CD172a:FITC
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
FC/FACS
Pricing
FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of A431 cells using anti-BIK antibody (AAA11666).Overlay histogram showing A431 cells stained with AAA11666 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BIK Antibody (AAA11666,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Bik, Polyclonal Antibody (Cat# AAA11666)

Full Name
Anti-Bik Antibody
Gene Names
BIK; BP4; NBK; BIP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using CAIX-Monospecific Mouse Monoclonal Antibody (CA9/3406). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Renal Cell Carcinoma, Monoclonal Antibody (Cat# AAA23952)

Full Name
Renal Cell Carcinoma (Carbonic Anhydrase IX)
Gene Names
CA9; MN; CAIX
Reactivity
Human
Applications
FC/FACS, IHC, WB
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U20S cells using anti-Dynamin 1 antibody (AAA19160).Overlay histogram showing U20S cells stained with AAA19160 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNM1 Antibody (AAA19160,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Dynamin 1, Polyclonal Antibody (Cat# AAA19160)

Full Name
Anti-Dynamin 1 Picoband antibody
Gene Names
DNM1; DNM; EIEE31
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling DLL3 with (1:25), followed by Dylight 488- conjugated goat anti- rabbit IgG secondary antibody at (1:200) (green). Immunofluorescence image showing nucleus and weak cytoplasm staining on U-2 OScell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (1:100) (red).)

DLL3, Polyclonal Antibody (Cat# AAA26850)

Full Name
DLL3, CT (DLL3, Delta-like protein 3, Drosophila Delta homolog 3) (APC)
Gene Names
DLL3; SCDO1
Reactivity
Human
Applications
IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

MCM2, Polyclonal Antibody (Cat# AAA31460)

Full Name
Phospho-MCM2 (Ser27) Antibody
Gene Names
MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

AXL, Polyclonal Antibody (Cat# AAA31469)

Full Name
Phospho-AXL (Tyr702) Antibody
Gene Names
AXL; UFO; JTK11
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (Published customer image:Phycoerythrin conjugated Mouse anti Human CD83 antibody, clone HB15e used for the evaluation of CD83 expression on monocyte derived dendritic cells by flow cytometry.Phenotypic characterization of immunogenic and tolerogenic moDC populations by flow cytometry. Monocytes were negatively selected from PBMC using magnetic beads. Immature moDC were generated with IL-4 and GM-CSF for 6 days. 15d-PGJ2 (PGJ2 DC) and dexamethasone plus 1alpha,25-dihydroxyvitamin were added to generate tolerogenic moDC, respectively (PGJ2 DC and Dex/VD3 DC). To generate immunogenic moDC, immature moDC were stimulated for 24 h with LPS, polyI:C and a cytokine cocktail containing TNF-alpha, IL-1beta, IL-6 and PGE2, respectively. The phenotypes of the cells were analyzed by flow cytometry. Live cells were gated according to FSC/SSC. One representative experiment out of three is shown.From: Sprater F, Hovden A-O, Appel S (2012)Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells.PLoS ONE 7(11): e49577.)

CD83, Monoclonal Antibody (Cat# AAA12282)

Full Name
MOUSE ANTI HUMAN CD83
Reactivity
Human
Applications
FC/FACS, IHC, IHC, IP
Pricing
FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of U937 cells using anti-NSF antibody (AAA19222).Overlay histogram showing U937 cells stained with AAA19222 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NSF Antibody (AAA19222, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

NSF, Polyclonal Antibody (Cat# AAA19222)

Full Name
Anti-NSF Antibody
Gene Names
NSF; SKD2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence) (Figure 6. IF analysis of EEF2 using anti- EEF2 antibody (AAA19228).EEF2 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-EEF2 Antibody (AAA19228) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

EEF2, Polyclonal Antibody (Cat# AAA19228)

Full Name
Anti-EEF2 Antibody
Gene Names
EEF2; EF2; EF-2; EEF-2
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, EIA
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry) (PCNA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

PCNA, Polyclonal Antibody (Cat# AAA28705)

Full Name
PCNA Antibody (C-term)
Gene Names
PCNA; ATLD2
Reactivity
Human (Predicted Reactivity: Bovine, Hamster, Monkey, Rat)
Applications
EIA, IHC, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data (Analysis of Protein Array containing >19, 000 full-length human proteins using EpCAM Mouse Monoclonal Antibody (EGP40/1372) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Ep-CAM/CD326, Monoclonal Antibody (Cat# AAA23890)

Full Name
Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker)
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human. Others not known.
Applications
FC/FACS, IF, WB, IHC
Pricing
Application Data (Detect RNase residue by agarose gel electrophores)

Proteinase K-lyophilization, Protein (Cat# AAA28067)

Full Name
Proteinase K-lyophilization
Purity
>=95% by SDS-PAGE
Pricing
FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U-87 cells using anti-ATP citrate lyase antibody (AAA11689).Overlay histogram showing U-87 cells stained with AAA11689 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP citrate lyase Antibody (AAA11689,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ATP citrate lyase, Polyclonal Antibody (Cat# AAA11689)

Full Name
Anti-ATP citrate lyase Antibody
Gene Names
ACLY; ACL; ATPCL; CLATP
Reactivity
Human, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)

JAK3, Polyclonal Antibody (Cat# AAA31404)

Full Name
Phospho-JAK3 (Tyr904) Antibody
Gene Names
JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%), Sheep (100%), Dog (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)

JAK3, Polyclonal Antibody (Cat# AAA31405)

Full Name
Phospho-JAK3 (Tyr981) Antibody
Gene Names
JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (100%), Bovine (91%), Horse (100%), Sheep (100%), Dog (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

FRS2, Polyclonal Antibody (Cat# AAA31412)

Full Name
Phospho-FRS2 (Tyr196) Antibody
Gene Names
FRS2; SNT; SNT1; FRS2A; SNT-1; FRS2alpha
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

CD22, Polyclonal Antibody (Cat# AAA31418)

Full Name
Phospho-CD22 (Tyr842) Antibody
Gene Names
CD22; SIGLEC2; SIGLEC-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Horse (83%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)

CD25, Monoclonal Antibody (Cat# AAA11966)

Full Name
MOUSE ANTI RAT CD25
Gene Names
Il2ra; IL2RAC
Applications
EIA, FC/FACS, IP
Pricing
Application Data (Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)

CD11b, Monoclonal Antibody (Cat# AAA11971)

Full Name
MOUSE ANTI RAT CD11b
Gene Names
ITGAM; CD11B
Applications
FC/FACS, IF, IP
Pricing
FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U-87 cells using anti-MVP antibody (AAA19137).Overlay histogram showing U-87 cells stained with AAA19137 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MVP Antibody (AAA19137,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MVP, Polyclonal Antibody (Cat# AAA19137)

Full Name
Anti-MVP Picoband antibody
Gene Names
MVP; LRP; VAULT1
Reactivity
Human, Mouse, Rat
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Cyclin E1, Polyclonal Antibody (Cat# AAA31439)

Full Name
Phospho-Cyclin E1 (Ser399) Antibody
Gene Names
CCNE1; CCNE
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (88%), Horse (88%), Sheep (88%), Chicken (88%), Xenopus (88%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot) (Western blot analysis of PAK1 expression in Etoposide treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

PAK1, Polyclonal Antibody (Cat# AAA31096)

Full Name
PAK1 Antibody
Gene Names
PAK1; PAKalpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

F4/80, Monoclonal Antibody (Cat# AAA12165)

Full Name
RAT ANTI MOUSE F4/80:Low Endotoxin
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
FC/FACS, FN, IP, RIA, RE, WB
Pricing
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

F4/80, Monoclonal Antibody (Cat# AAA12170)

Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
EM, FC/FACS, IF, IP, RIA, RE, WB
Pricing
WB (Western Blot) (Western BlotSample: Recombinant GAL9, Human;Antibody: Rabbit Anti-Human GAL9 Ab)

Galectin 9 (GAL9), Active Protein (Cat# AAA21103)

Full Name
Active Galectin 9 (GAL9)
Gene Names
LGALS9; HUAT; LGALS9A
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>90%
Pricing
Application Data (Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)

CD11b, Monoclonal Antibody (Cat# AAA12146)

Full Name
MOUSE ANTI RAT CD11b:FITC
Gene Names
ITGAM; CD11B
Applications
FC/FACS
Pricing

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