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8519 results for "Protein" - showing 8050-8100
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA26864)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (MaxLight 750)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA26863)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (MaxLight 650)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)
OPN-a/b, Polyclonal Antibody (Cat# AAA26862)
Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (MaxLight 550)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
Application Data
(Published customer image:Phycoerythrin conjugated Mouse anti Human CD83 antibody, clone HB15e used for the evaluation of CD83 expression on monocyte derived dendritic cells by flow cytometry.Phenotypic characterization of immunogenic and tolerogenic moDC populations by flow cytometry. Monocytes were negatively selected from PBMC using magnetic beads. Immature moDC were generated with IL-4 and GM-CSF for 6 days. 15d-PGJ2 (PGJ2 DC) and dexamethasone plus 1alpha,25-dihydroxyvitamin were added to generate tolerogenic moDC, respectively (PGJ2 DC and Dex/VD3 DC). To generate immunogenic moDC, immature moDC were stimulated for 24 h with LPS, polyI:C and a cytokine cocktail containing TNF-alpha, IL-1beta, IL-6 and PGE2, respectively. The phenotypes of the cells were analyzed by flow cytometry. Live cells were gated according to FSC/SSC. One representative experiment out of three is shown.From: Sprater F, Hovden A-O, Appel S (2012)Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells.PLoS ONE 7(11): e49577.)
CD83, Monoclonal Antibody (Cat# AAA12281)
Full Name
MOUSE ANTI HUMAN CD83: FITC
Reactivity
Human
Applications
FC/FACS
Pricing
IF (Immunofluorescence)
(Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG1) (Green).DAPI was used to stain the cell nuclei (blue). (Negative Control))
Ep-CAM /CD326, Monoclonal Antibody (Cat# AAA13838)
Full Name
Ep-CAM /CD326 (Epithelial Marker) Mouse Monoclonal Antibody
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human.
Does not react with Mouse and Rat
Does not react with Mouse and Rat
Applications
FC/FACS, IF, WB, IHC
Pricing
SDS-PAGE
(SDS-PAGE Analysis Purified with Nucleolin Monoclonal Antibody (364-5). Confirmation of Integrity and Purity of Antibody)
Nucleolin, Monoclonal Antibody (Cat# AAA13835)
Full Name
Nucleolin (Marker of Human Cells) Mouse Monoclonal Antibody
Gene Names
NCL; C23
Reactivity
Does not react with Mouse, Human, Rat and Cow
Applications
FC/FACS, IF, WB, IHC
Pricing
WB (Western Blot)
(Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)
Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26902)
Full Name
Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol
Gene Names
PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha
Reactivity
Human
Applications
ICC, WB, IP, IHC
Purity
Purified by Immunoaffinity chromatography.
Pricing
IHC (Immunohistchemistry)
(Figure 9 Immunohistochemistry Validation of hRIP3 in Human Colon Tissue Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-hRIP3 antibody (8963) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
hRIP3, Polyclonal Antibody (Cat# AAA11031)
Full Name
hRIP3 Antibody
Gene Names
RIPK3; RIP3
Reactivity
Human
Applications
EIA, IF, IHC, WB
Purity
hRIP3 Antibody is Protein A purified.
Pricing
WB (Western Blot)
(Western blot analysis of lysates from Hela, HepG2, MCF-7, SH-SY5Y, mouse NIH/3T3 cell line (from left to right), using FGFR1 Antibody (Center). AAA28687 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
FGFR1, Polyclonal Antibody (Cat# AAA28687)
Full Name
FGFR1 Antibody (Center)
Gene Names
FGFR1; CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1
Reactivity
Human, mouse (Predicted Reactivity: Chicken, Rat)
Applications
FC/FACS, EIA, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).)
MUC1 / EMA /CD227, Monoclonal Antibody (Cat# AAA13841)
Full Name
MUC1 / EMA /CD227 (Epithelial Marker) Mouse Monoclonal Antibody
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Human
Applications
FC/FACS, IF, IHC
Pricing
WB (Western Blot)
(HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in Raw 264.7.)
HSPA1A, Monoclonal Antibody (Cat# AAA25129)
Full Name
HSPA1A (Heat Shock 70kD Protein 1A/1B, Heat Shock 70kD Protein 1/2, HSP70.1/HSP70.2, HSP70-1/HSP70-2, HSPA1, HSPA1B) (FITC)
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
Reactivity
Human, Mouse, Rat
Applications
EIA, IF, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing HepG2 cells stained with AAA28061 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
CD63, Monoclonal Antibody (Cat# AAA28061)
Full Name
CD63 Monoclonal Antibody
Gene Names
Cd63; ME491; C75951; Tspan30
Reactivity
Human
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
>95%, Protein A purified
Pricing
WB (Western Blot)
(HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in Raw 264.7.)
HSPA1A, Monoclonal Antibody (Cat# AAA24241)
Full Name
HSPA1A (Heat Shock 70kD Protein 1A/1B, Heat Shock 70kD Protein 1/2, HSP70.1/HSP70.2, HSP70-1/HSP70-2, HSPA1, HSPA1B) (AP)
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of GALE using anti-GALE antibody (AAA19135).GALE was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GALE Antibody (AAA19135) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
GALE, Polyclonal Antibody (Cat# AAA19135)
Full Name
Anti-GALE Picoband antibody
Gene Names
GALE; SDR1E1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of Cdc20 using anti-Cdc20 antibody (AAA19132).Cdc20 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Cdc20 Antibody (AAA19132) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Cdc20/P55 Cdc, Polyclonal Antibody (Cat# AAA19132)
Full Name
Anti-Cdc20/P55 Cdc Picoband Antibody
Gene Names
CDC20; CDC20A; p55CDC; bA276H19.3
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
Application Data
(Staining of mouse spleen with Rat anti Mouse CD4:RPE)
CD4, Monoclonal Antibody (Cat# AAA12227)
Full Name
RAT ANTI MOUSE CD4
Gene Names
Cd4; L3T4; Ly-4
Applications
EIA, FC/FACS, IF, IP, WB
Pricing
Application Data
(Staining of mouse spleen with Rat anti Mouse CD4:RPE)
CD4, Monoclonal Antibody (Cat# AAA12230)
Full Name
RAT ANTI MOUSE CD4
Gene Names
Cd4; L3T4; Ly-4
Applications
EIA, FC/FACS, IF, IP, WB
Pricing
CD169, Monoclonal Antibody (Cat# AAA11972)
Full Name
MOUSE ANTI RAT CD169
Applications
FC/FACS, IF, IP
Pricing
Application Data
(Staining of mouse spleen with Rat anti Mouse CD4:RPE)
CD4, Monoclonal Antibody (Cat# AAA12233)
Full Name
RAT ANTI MOUSE CD4
Gene Names
Cd4; L3T4; Ly-4
Applications
EIA, FC/FACS, IF, IP, WB
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of PDPK1 using anti-PDPK1 antibody (AAA11643).PDPK1 was detected in frozen section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PDPK1 Antibody (AAA11643) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
PDPK1, Polyclonal Antibody (Cat# AAA11643)
Full Name
Anti-PDPK1 Antibody
Gene Names
PDPK1; PDK1; PDPK2; PDPK2P; PRO0461
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
DB (Dot Blot)
(Dot blot analysis using Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507). Tissue: Cell lysates. Species: Human. Primary Antibody: Rabbit Anti-Amyloid Fibrils (OC) Polyclonal Antibody (SPC-507) at 1:500, 1:5000. Beta Amyloid HEPES-NaCl aggregation, showing 1:500 (L) and 1:5000 (R) time lapse dot blot.)
Amyloid Fibrils (OC), Polyclonal Antibody (Cat# AAA17803)
Full Name
Amyloid Fibrils (OC) Antibody: ATTO 488
Reactivity
Human. Potentially mouse and rat based on species homology.
Applications
IP, ICC, IF, IHC, EIA, WB, DB
Purity
Protein A Purified
Pricing
IHC (Immunohistchemistry)
(Formalin-paraffin human Placenta stained with E-Cadherin MAb (CDH1/1525).)
E-Cadherin/CD324, Monoclonal Antibody (Cat# AAA23896)
Full Name
E-Cadherin/CD324 (Intercellular Junction Marker)
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
Reactivity
Human.
Does not react with Mouse and Rat. Others not known.
Does not react with Mouse and Rat. Others not known.
Applications
FC/FACS, IF, WB, IHC
Pricing
WB (Western Blot)
(Western blot analysis of Catenin-gamma expression in mouse tissue extract)
Catenin-gamma, Polyclonal Antibody (Cat# AAA31082)
Full Name
Catenin-gamma Antibody
Gene Names
JUP; DP3; PDGB; PKGB; CTNNG; DPIII
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of Integrin alpha 5 using anti-Integrin alpha 5 antibody (AAA19156).Integrin alpha 5 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Integrin alpha 5 Antibody (AAA19156) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Integrin alpha 5, Polyclonal Antibody (Cat# AAA19156)
Full Name
Anti-Integrin alpha 5 Picoband antibody
Gene Names
ITGA5; FNRA; CD49e; VLA-5; VLA5A
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of HE4 using anti-HE4 antibody (AAA19161).HE4 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HE4 Antibody (AAA19161) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
HE4, Polyclonal Antibody (Cat# AAA19161)
Full Name
Anti-HE4 Picoband Antibody
Gene Names
Wfdc2; re4
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of LGALS3BP using anti-LGALS3BP antibody (AAA19163).LGALS3BP was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LGALS3BP Antibody (AAA19163) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
LGALS3BP, Polyclonal Antibody (Cat# AAA19163)
Full Name
Anti-LGALS3BP Picoband Antibody
Reactivity
Human, Mouse
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Pricing
IHC (Immunohistochemistry)
(Figure 8. IHC analysis of GSTM3 using anti-GSTM3 antibody (AAA19168).GSTM3 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GSTM3 Antibody (AAA19168) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
GSTM3, Polyclonal Antibody (Cat# AAA19168)
Full Name
Anti-GSTM3 Picoband antibody
Gene Names
GSTM3; GST5; GSTB; GTM3; GSTM3-3
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of Jurkat cells using anti-Ki67 antibody (AAA19350).Overlay histogram showing Jurkat cells stained with AAA19350 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Ki67 Antibody (AAA19350, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Ki67, Monoclonal Antibody (Cat# AAA19350)
Full Name
Anti-Ki67 Antibody (monoclonal, 5E12)
Gene Names
MKI67; KIA
Reactivity
Human
Applications
IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
Application Data
(Staining of canine peripheral blood lymphocytes with Rat anti Canine CD4: Alexa Fluor 488)
CD4, Monoclonal Antibody (Cat# AAA12064)
Full Name
RAT ANTI DOG CD4:FITC
Applications
FC/FACS
Pricing
FCM (Flow Cytometry)
(GTF2I Antibody (C-term) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
GTF2I, Polyclonal Antibody (Cat# AAA28707)
Full Name
GTF2I Antibody (C-term)
Gene Names
GTF2I; WBS; DIWS; SPIN; IB291; BAP135; BTKAP1; TFII-I; WBSCR6; GTFII-I
Reactivity
Human (Predicted Reactivity: Rat)
Applications
IF, EIA, IHC, FC/FACS, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western Blot Analysis of Human Spleen cell lysate using HLA-Pan Mouse Monoclonal Antibody (CR3/43).)
HLA-DP/-DQ/-DR, Monoclonal Antibody (Cat# AAA23889)
Full Name
HLA-DP/-DQ/-DR (MHC II)
Gene Names
HLA-DPB1; DPB1; HLA-DP; HLA-DPB; HLA-DP1B
Reactivity
Human
Applications
IF, IHC
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ERCC1 Mouse Monoclonal Antibody (ERCC1/2318). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
ERCC1/RAD10, Monoclonal Antibody (Cat# AAA23913)
Full Name
ERCC1/RAD10 (Tumor Progression Marker)
Gene Names
ERCC1; UV20; COFS4; RAD10
Reactivity
Human
Applications
IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Application Data
(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human CD44 antibody followed by HRP-polymer detection and DAB substrate development (high power) following antigen retrieval using citrate buffer at pH6.2)
CD44, Monoclonal Antibody (Cat# AAA11893)
Full Name
MOUSE ANTI HUMAN CD44:FITC
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Applications
FC/FACS, IHC-F/P, IF
Purity
Purified
Pricing
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Mesothelin Mouse Monoclonal Antibody (MSLN/2131). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mesothelin, Monoclonal Antibody (Cat# AAA23897)
Full Name
Mesothelin (Mesothelial Marker)
Gene Names
MSLN; MPF; SMRP
Reactivity
Human, Mouse, Rat. Others not known.
Applications
IHC
Pricing
Application Data
(Analysis of Protein Array containing >19, 000 full-length human proteins using TACSTD2 Mouse Monoclonal Antibody (TACSTD2/2153) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
TACSTD2/TROP2, Monoclonal Antibody (Cat# AAA23900)
Full Name
TACSTD2/TROP2 (Epithelial Marker)
Gene Names
TACSTD2; EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1
Reactivity
Human. Others not known.
Applications
IHC
Pricing
IHC (Immunohistchemistry)
(Figure 9. IHC analysis of BAK using anti-BAK antibody (AAA11654).BAK was detected in frozen section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BAK Antibody (AAA11654) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
BAK, Polyclonal Antibody (Cat# AAA11654)
Full Name
Anti-BAK Antibody
Gene Names
BAK1; BAK; CDN1; BCL2L7; BAK-LIKE
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating GAPDH in Hela whole cell lysateLane 1: Mouse control IgG instead of in Hela whole cell lysate. Lane 2: (5ul) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (10ug)For western blotting, the blot was detected at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000)
GAPDH, Monoclonal Antibody (Cat# AAA27042)
Full Name
GAPDH Monoclonal Antibody
Reactivity
Human, Mouse, Rabbit
Applications
EIA, WB, IHC, IP, IF
Purity
>95%, Protein G purified
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing MCF-7 cells stained with (red line). The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the primary antibody at 1/200 for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG1 used under the same conditions. Acquisition of >10,000 events was performed.)
HSPA8, Monoclonal Antibody (Cat# AAA27046)
Full Name
HSPA8 Monoclonal Antibody
Gene Names
HSPA8; LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; NIP71; HSPA10
Reactivity
Human
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
>95%, Protein G purified
Pricing
IHC (Immunohistochemistry)
(Figure 8. IHC analysis of RbAp48 using anti-RbAp48 antibody (AAA11671).RbAp48 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RbAp48 Antibody (AAA11671) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
RbAp48, Polyclonal Antibody (Cat# AAA11671)
Full Name
Anti-RbAp48 Antibody
Gene Names
RBBP4; NURF55; RBAP48; lin-53
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
Application Data
NMDA NR2A Subunit, Polyclonal Antibody (Cat# AAA14232)
Full Name
Anti-NMDA Receptor, NR2A Subunit
Gene Names
Grin2a; NR2A; GluN2A; NMDAR2A
Reactivity
Rat, mouse, human
Applications
WB, IHC, IP
Purity
Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.)
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Mnk1, Polyclonal Antibody (Cat# AAA31406)
Full Name
Phospho-Mnk1 (Thr250) Antibody
Gene Names
MKNK1; MNK1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Staining of mouse spleen with Rat anti Mouse CD4:RPE)
CD4, Monoclonal Antibody (Cat# AAA12228)
Full Name
RAT ANTI MOUSE CD4:FITC
Gene Names
Cd4; L3T4; Ly-4
Applications
FC/FACS
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of GLO1 using anti-GLO1 antibody (AAA19155).GLO1 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GLO1 Antibody (AAA19155) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
GLO1/Glyoxalase I, Polyclonal Antibody (Cat# AAA19155)
Full Name
Anti-GLO1/Glyoxalase I Picoband antibody
Gene Names
GLO1; GLYI; GLOD1; HEL-S-74
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IF (Immunofluorescence)
(AAA30923 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Galectin 3, Polyclonal Antibody (Cat# AAA30923)
Full Name
Galectin 3 Antibody
Gene Names
LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (AAA19147).VEGF Receptor 3 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VEGF Receptor 3 Antibody (AAA19147) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
VEGF Receptor 3, Polyclonal Antibody (Cat# AAA19147)
Full Name
Anti-VEGF Receptor 3 Picoband antibody
Gene Names
FLT4; PCL; FLT-4; FLT41; LMPH1A; VEGFR3; VEGFR-3
Reactivity
Human, Mouse, Rat
Applications
EIA, IHC, WB, FC
Purity
Immunoggen affinity purified
Pricing
IHC (Immunohistochemistry)
(Figure 10. IHC analysis of COX IV using anti-COX IV antibody (AAA19173).COX IV was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-COX IV Antibody (AAA19173) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
COX IV, Polyclonal Antibody (Cat# AAA19173)
Full Name
Anti-COX IV Picoband Antibody
Gene Names
COX4I1; COX4; COXIV; COX4-1; COXIV-1; COX IV-1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of Annexin VI using anti-Annexin VI antibody (AAA19170).Annexin VI was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Annexin VI Antibody (AAA19170) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Annexin VI, Polyclonal Antibody (Cat# AAA19170)
Full Name
Anti-Annexin VI Picoband Antibody
Gene Names
ANXA6; ANX6; CBP68
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of TSPAN12 using anti-TSPAN12 antibody (AAA19174).TSPAN12 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TSPAN12 Antibody (AAA19174) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
TSPAN12, Polyclonal Antibody (Cat# AAA19174)
Full Name
Anti-TSPAN12 Picoband antibody
Gene Names
TSPAN12; EVR5; NET2; NET-2; TM4SF12
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of Musashi 1/Msi1 using anti- Musashi 1/Msi1 antibody (AAA19172).Musashi 1/Msi1 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Musashi 1/Msi1 Antibody (AAA19172) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Musashi 1/Msi1, Polyclonal Antibody (Cat# AAA19172)
Full Name
Anti-Musashi 1/Msi1 Picoband Antibody
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of MED4 using anti-MED4 antibody (AAA19178).MED4 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MED4 Antibody (AAA19178) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
MED4, Polyclonal Antibody (Cat# AAA19178)
Full Name
Anti-MED4 Picoband Antibody
Gene Names
MED4; ARC36; VDRIP; DRIP36; TRAP36; HSPC126
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
