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8519 results for "Protein" - showing 8100-8150

IF (Immunofluorescence) (Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling Pdx1 with at 1:25 dilution, followed by DyLight 488-conjugated IgG goat anti-rabbit secondary antibody at 1:200 dilution (green). Immunofluorescence image showing cytoplasm staining on MCF-7 cell line. Cytoplasmic actin is detected with DyLight 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).)

OPN-a/b, Polyclonal Antibody (Cat# AAA26858)

Full Name
OPN-a/b, NT (SPP1, BNSP, OPN, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin) (Biotin)
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human
Applications
WB, IHC, IF, EIA
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
FCM (Flow Cytometry) (Figure 2. Flow Cytometry analysis of A549 cells using anti-WEE1 antibody (AAA19240).Overlay histogram showing A549 cells stained with AAA19240 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WEE1 Antibody (AAA19240, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WEE1, Polyclonal Antibody (Cat# AAA19240)

Full Name
Anti-WEE1 Antibody
Gene Names
WEE1; WEE1A; WEE1hu
Reactivity
Human, Mouse
Applications
WB, FC, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot) (WB Suggested Anti-GSR Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Hela cell lysate)

GSR, Polyclonal Antibody (Cat# AAA23553)

Full Name
GSR antibody - N-terminal region
Gene Names
GSR; GR; HEL-75; HEL-S-122m
Reactivity
Tested Reactivity: Human, Mouse, Zebrafish
Predicted Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
IHC, WB
Purity
Affinity Purified
Pricing
FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HL-60 cells using anti-Sumo 1/SUMO1 antibody (AAA19224).Overlay histogram showing HL-60 cells stained with AAA19224 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sumo 1/SUMO1 Antibody (AAA19224, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Sumo 1/SUMO1, Polyclonal Antibody (Cat# AAA19224)

Full Name
Anti-Sumo 1/SUMO1 Antibody
Gene Names
SUMO1; DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3
Reactivity
Human, Mouse, Rat
Applications
IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot) (WB Suggested Anti-SHH Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysate)

SHH, Polyclonal Antibody (Cat# AAA23516)

Full Name
SHH antibody - N-terminal region
Gene Names
SHH; TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5
Reactivity
Predicted Reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish, Chicken (Tested Reactivity: Human, Mouse, Chicken)
Applications
IHC, WB
Purity
Affinity Purified
Pricing
FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of PC-3 cells using anti-ATG14L antibody (AAA11658).Overlay histogram showing PC-3 cells stained with AAA11658 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATG14L Antibody (AAA11658,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ATG14L, Polyclonal Antibody (Cat# AAA11658)

Full Name
Anti-ATG14L Antibody
Gene Names
ATG14; ATG14L; BARKOR; KIAA0831
Reactivity
Human, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
WB (Western Blot) (Western Blot Analysis of Raji cell lysate using CD20 Mouse Monoclonal Antibody (L26).)

CD20/MS4A1, Monoclonal Antibody (Cat# AAA23946)

Full Name
CD20/MS4A1 (B-Cell Marker)
Gene Names
MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
Reactivity
Human
Applications
FC/FACS, IF, WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

F4/80, Monoclonal Antibody (Cat# AAA12171)

Full Name
RAT ANTI MOUSE F4/80:RPE
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
FC/FACS
Pricing
WB (Western Blot) (Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3194).)

Prohibitin, Monoclonal Antibody (Cat# AAA23932)

Full Name
Prohibitin (Mitochondrial Marker)
Gene Names
PHB; PHB1; HEL-215; HEL-S-54e
Reactivity
Human
Applications
WB, IF, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

F4/80, Monoclonal Antibody (Cat# AAA12217)

Full Name
RAT ANTI MOUSE F4/80:APC
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
FC/FACS
Pricing
IHC (Immunohistochemistry) (Figure 8. IHC analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (AAA19146).VEGF Receptor 3 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-VEGF Receptor 3 Antibody (AAA19146) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

VEGF Receptor 3, Polyclonal Antibody (Cat# AAA19146)

Full Name
Anti-VEGF Receptor 3 Picoband Antibody
Gene Names
FLT4; PCL; FLT-4; FLT41; LMPH1A; VEGFR3; VEGFR-3
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Cytokeratin 18, Polyclonal Antibody (Cat# AAA31327)

Full Name
Phospho-Cytokeratin 18 (Ser51) Antibody
Gene Names
KRT18; K18; CYK18
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Twist2, Polyclonal Antibody (Cat# AAA31341)

Full Name
Twist2 Antibody
Gene Names
TWIST2; AMS; FFDD3; BBRSAY; DERMO1; SETLSS; bHLHa39
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (92%), Dog (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of SAE2/UBA2 using anti-SAE2/UBA2 antibody (AAA19171).SAE2/UBA2 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SAE2/UBA2 Antibody (AAA19171) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

SAE2/UBA2, Polyclonal Antibody (Cat# AAA19171)

Full Name
Anti-SAE2/UBA2 Picoband antibody
Gene Names
UBA2; ARX; SAE2; HRIHFB2115
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of DYNLT1 using anti-DYNLT1 antibody (AAA19175).DYNLT1 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DYNLT1 Antibody (AAA19175) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

DYNLT1, Polyclonal Antibody (Cat# AAA19175)

Full Name
Anti-DYNLT1 Picoband antibody
Gene Names
DYNLT1; CW-1; TCTEL1; tctex-1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IHC (Immunohistochemistry) (Figure 7. IHC analysis of GALNS using anti-GALNS antibody (AAA19164).GALNS was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GALNS Antibody (AAA19164) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

GALNS, Antibody (Cat# AAA19164)

Full Name
Anti-GALNS Picoband antibody
Gene Names
GALNS; GAS; MPS4A; GalN6S; GALNAC6S
Reactivity
Reacts with: Human, Mouse, Rat
Applications
WB, EIA
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of Thrombopoietin using anti- Thrombopoietin antibody (AAA19166).Thrombopoietin was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Thrombopoietin Antibody (AAA19166) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Thrombopoietin, Polyclonal Antibody (Cat# AAA19166)

Full Name
Anti-Thrombopoietin Picoband Antibody
Gene Names
Thpo; Ml; Tpo; Mgdf; Mpllg
Reactivity
Mouse, Rat
No cross reactivity with other proteins
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of K562 cells using anti-D2AP antibody (M01756). Overlay histogram showing K562 cells stained with M01756 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-D2AP Antibody (M01756, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

CD2AP, Monoclonal Antibody (Cat# AAA19185)

Full Name
Anti-CD2AP Antibody (monoclonal, 5F8)
Gene Names
CD2AP; CMS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
IHC (Immunohistchemistry) (Figure 5. IHC analysis of GNG4 using anti-GNG4 antibody (AAA19341).GNG4 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG4 Antibody (AAA19341) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

GNG4, Polyclonal Antibody (Cat# AAA19341)

Full Name
Anti-GNG4 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of K562 cells using anti-IRF3 antibody (AAA19216).Overlay histogram showing K562 cells stained with AAA19216 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IRF3 Antibody (AAA19216, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IRF3, Polyclonal Antibody (Cat# AAA19216)

Full Name
Anti-IRF3 Antibody
Reactivity
Human
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot) (PLD6 Antibody (Center) (Cat# AAA28684) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the PLD6 antibody detected the PLD6 protein (arrow).)

PLD6, Polyclonal Antibody (Cat# AAA28684)

Full Name
PLD6 Antibody (Center)
Gene Names
PLD6; ZUC
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data (Published clone specific image: Flow cytometric analysis of AM from NO2-exposed and control rats. Rats were exposed to NO2 for the indicated times and BAL cells were stained with antibodies to ED7, ED9, RM-4, and OX-6. To overcome autofluorescence signals, primary antibodies were detected using a biotin-PE/streptavidin-anti-streptavidin enhancing system and labeling of AM was analyzed by flow cytometry following gating by help of forward and sideward scatter properties. Shown are representative results of at least six animals per group.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)

CD172a, Monoclonal Antibody (Cat# AAA12056)

Full Name
MOUSE ANTI RAT CD172a:RPE
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
FC/FACS
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Jak2, Polyclonal Antibody (Cat# AAA31311)

Full Name
Phospho-Jak2 (Tyr1008) Antibody
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2959). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Cytokeratin 15, Monoclonal Antibody (Cat# AAA23924)

Full Name
Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker)
Gene Names
KRT15; K15; CK15; K1CO
Reactivity
Human
Applications
IF, WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
WB (Western Blot) (Western blot analysis of HDAC5 expression in mouse brain tissue lysates, The lane on the right is treated with the antigen-specific peptide.)

HDAC5, Polyclonal Antibody (Cat# AAA31097)

Full Name
HDAC5 Antibody
Gene Names
HDAC5; HD5; NY-CO-9
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

MCM2, Polyclonal Antibody (Cat# AAA31328)

Full Name
Phospho-MCM2 (Ser108) Antibody
Gene Names
MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

F4/80, Monoclonal Antibody (Cat# AAA12161)

Full Name
RAT ANTI MOUSE F4/80:APC
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
FC/FACS
Pricing
WB (Western Blot) (Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3225).)

Prohibitin, Monoclonal Antibody (Cat# AAA23933)

Full Name
Prohibitin (Mitochondrial Marker)
Gene Names
PHB; PHB1; HEL-215; HEL-S-54e
Reactivity
Human
Applications
WB, IF, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
Application Data (Staining of KG1 lymphocytes with Mouse anti Human CD59:FITC)

CD59, Monoclonal Antibody (Cat# AAA11916)

Full Name
MOUSE ANTI HUMAN CD59
Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Applications
EIA, EM, FC/FACS, IF, IP, WB
Pricing
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

F4/80, Monoclonal Antibody (Cat# AAA12172)

Full Name
RAT ANTI MOUSE F4/80:RPE
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
FC/FACS, IF
Pricing
Application Data (Staining of KG1 lymphocytes with Mouse anti Human CD59:FITC)

CD59, Monoclonal Antibody (Cat# AAA11917)

Full Name
MOUSE ANTI HUMAN CD59
Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Applications
EIA, EM, FC/FACS, IF, IP, WB
Pricing
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

F4/80, Monoclonal Antibody (Cat# AAA12163)

Full Name
RAT ANTI MOUSE F4/80:Biotin
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
FC/FACS
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody (AAA19130).Flt3 / CD135 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Flt3 / CD135 Antibody (AAA19130) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Flt3/CD135, Polyclonal Antibody (Cat# AAA19130)

Full Name
Anti-Flt3/CD135 Picoband Antibody
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry) (Figure 8. IHC analysis of beta Catenin using anti-beta Catenin antibody (AAA19129).beta Catenin was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-beta Catenin Antibody (AAA19129) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

beta Catenin, Polyclonal Antibody (Cat# AAA19129)

Full Name
Anti-beta Catenin Picoband Antibody
Gene Names
CTNNB1; EVR7; CTNNB; MRD19; armadillo
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
FCM (Flow Cytometry) (Overlay histogram showing Hela cells stained with (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)

YWHAZ, Monoclonal Antibody (Cat# AAA27050)

Full Name
YWHAZ Monoclonal Antibody
Gene Names
YWHAZ; YWHAD; KCIP-1; 14-3-3-zeta
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
>95%, Protein A purified
Pricing
FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of THP-1 cells using anti-TLR1 antibody (AAA19134).Overlay histogram showing THP-1 cells stained with AAA19134 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TLR1 Antibody (AAA19134,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

TLR1, Polyclonal Antibody (Cat# AAA19134)

Full Name
Anti-TLR1 Picoband antibody
Gene Names
TLR1; TIL; CD281; rsc786; TIL. LPRS5
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of HP1 gamma using anti-HP1 gamma antibody (AAA19145).HP1 gamma was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HP1 gamma Antibody (AAA19145) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

HP1 gamma, Polyclonal Antibody (Cat# AAA19145)

Full Name
Anti-HP1 gamma Picoband antibody
Gene Names
CBX3; HECH; HP1-GAMMA; HP1Hs-gamma
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of Cyclophilin B using anti-Cyclophilin B antibody (AAA19167).Cyclophilin B was detected in paraffin-embedded section of rat thyroid gland tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Cyclophilin B Antibody (AAA19167) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Cyclophilin B, Polyclonal Antibody (Cat# AAA19167)

Full Name
Anti-Cyclophilin B Picoband Antibody
Gene Names
PPIB; OI9; CYPB; SCYLP; CYP-S1; HEL-S-39
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

NDEL1, Polyclonal Antibody (Cat# AAA31462)

Full Name
Phospho-NDEL1 (Thr219) Antibody
Gene Names
NDEL1; EOPA; NDE2; NUDEL; MITAP1; NDE1L1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (Published customer image:Phycoerythrin conjugated Mouse anti Human CD83 antibody, clone HB15e used for the evaluation of CD83 expression on monocyte derived dendritic cells by flow cytometry.Phenotypic characterization of immunogenic and tolerogenic moDC populations by flow cytometry. Monocytes were negatively selected from PBMC using magnetic beads. Immature moDC were generated with IL-4 and GM-CSF for 6 days. 15d-PGJ2 (PGJ2 DC) and dexamethasone plus 1alpha,25-dihydroxyvitamin were added to generate tolerogenic moDC, respectively (PGJ2 DC and Dex/VD3 DC). To generate immunogenic moDC, immature moDC were stimulated for 24 h with LPS, polyI:C and a cytokine cocktail containing TNF-alpha, IL-1beta, IL-6 and PGE2, respectively. The phenotypes of the cells were analyzed by flow cytometry. Live cells were gated according to FSC/SSC. One representative experiment out of three is shown.From: Sprater F, Hovden A-O, Appel S (2012)Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells.PLoS ONE 7(11): e49577.)

CD83, Monoclonal Antibody (Cat# AAA12280)

Full Name
MOUSE ANTI HUMAN CD83
Reactivity
Human
Applications
FC/FACS, IHC, IHC, IP
Pricing
Application Data (Published customer image:Phycoerythrin conjugated Mouse anti Human CD83 antibody, clone HB15e used for the evaluation of CD83 expression on monocyte derived dendritic cells by flow cytometry.Phenotypic characterization of immunogenic and tolerogenic moDC populations by flow cytometry. Monocytes were negatively selected from PBMC using magnetic beads. Immature moDC were generated with IL-4 and GM-CSF for 6 days. 15d-PGJ2 (PGJ2 DC) and dexamethasone plus 1alpha,25-dihydroxyvitamin were added to generate tolerogenic moDC, respectively (PGJ2 DC and Dex/VD3 DC). To generate immunogenic moDC, immature moDC were stimulated for 24 h with LPS, polyI:C and a cytokine cocktail containing TNF-alpha, IL-1beta, IL-6 and PGE2, respectively. The phenotypes of the cells were analyzed by flow cytometry. Live cells were gated according to FSC/SSC. One representative experiment out of three is shown.From: Sprater F, Hovden A-O, Appel S (2012)Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells.PLoS ONE 7(11): e49577.)

CD83, Monoclonal Antibody (Cat# AAA12283)

Full Name
MOUSE ANTI HUMAN CD83
Reactivity
Human
Applications
FC/FACS, IHC, IHC
Pricing
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Hsp70 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

Hsp70, Monoclonal Antibody (Cat# AAA29972)

Full Name
Hsp70 Antibody
Gene Names
HSPA1B; HSP72; HSPA1; HSX70; HSP70-1; HSP70-2; HSP70.1; HSP70.2; HSP70-1B
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using UBE2I mouse mAb with DAB staining.)

Ubc9, Monoclonal Antibody (Cat# AAA14109)

Full Name
Anti-Ubc9 Mouse mAb
Gene Names
UBE2I; P18; UBC9; C358B7.1
Reactivity
Human, Mouse, Rat
Applications
WB
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human prostate cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

hFANCI, Polyclonal Antibody (Cat# AAA31282)

Full Name
Phospho-hFANCI (Ser565) Antibody
Gene Names
FANCI; KIAA1794
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IP (Immunoprecipitation) (Immunoprecipitating ENO1 in HepG2 whole cell lysate Lane 1: Mouse control IgG (1ug) instead of in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000) Lane 2: (1ul) + HepG2 whole cell lysate (500ug) Lane 3: HepG2 whole cell lysate (10ug))

ENO1, Monoclonal Antibody (Cat# AAA27044)

Full Name
ENO1 Monoclonal Antibody
Gene Names
ENO1; NNE; PPH; MPB1; ENO1L1
Reactivity
Human, Mouse, Rat, Rabbit
Applications
EIA, WB, IF, FC/FACS, IP
Purity
>95%, Protein G purified
Pricing
IP (Immunoprecipitation) (Immunoprecipitating PD-L2 in Hela whole cell lysate Lane 1: Mouse control IgG instead of in Hela whole cell lysate. Lane 2: (2ul) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (20ug) For western blotting, the blot was detected at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000 )

PD-L2, Monoclonal Antibody (Cat# AAA27047)

Full Name
PD-L2 Monoclonal Antibody
Gene Names
PDCD1LG2; B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
Reactivity
Human
Applications
EIA, WB, IHC, IF, FC/FACS, IP
Purity
>95%, Protein A purified
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human gastric cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

CAMKK1/2, Polyclonal Antibody (Cat# AAA31402)

Full Name
Phospho-CAMKK1/2 (Ser458/Ser495) Antibody
Gene Names
CAMKK1; CAMKKA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry) (Figure 8. IHC analysis of PRDM1/Blimp1 using anti-PRDM1/Blimp1 antibody (AAA19133).PRDM1/Blimp1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PRDM1/Blimp1 Antibody (AAA19133) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

PRDM1/Blimp1, Polyclonal Antibody (Cat# AAA19133)

Full Name
Anti-PRDM1/Blimp1 Picoband antibody
Gene Names
PRDM1; BLIMP1; PRDI-BF1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IHC (Immunohistochemistry) (At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

CtBP1/2, Polyclonal Antibody (Cat# AAA31423)

Full Name
Phospho-CtBP1/2 (Ser158/Ser164) Antibody
Gene Names
CTBP1; BARS
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (82%), Bovine (100%), Horse (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of NSF using anti-NSF antibody (AAA19136).NSF was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NSF Antibody (AAA19136) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

NSF, Polyclonal Antibody (Cat# AAA19136)

Full Name
Anti-NSF Picoband antibody
Gene Names
NSF; SKD2; SEC18
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing

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