Search results for "Cell Cycle"

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FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with CDK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

CDK1, Monoclonal Antibody (Cat# AAA30031)

Full Name

CDK1 Antibody

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IHC, FC/FACS

Purity

ProA affinity purified

Pricing

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WB (Western Blot) (WB Suggested Anti-CHEK1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Small Intestine)

CHEK1, Polyclonal Antibody (Cat# AAA23414)

Full Name

CHEK1 antibody - middle region

Gene Names

CHEK1; CHK1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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WB (Western Blot) (Western blot analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

CDC25C, Monoclonal Antibody (Cat# AAA24754)

Full Name

CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) (Biotin)

Gene Names

CDC25C; CDC25; PPP1R60

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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ICC (Immunocytochemistry) (ICC staining Cdk9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

CDK9, Monoclonal Antibody (Cat# AAA30211)

Full Name

CDK9 Antibody

Gene Names

CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE

Reactivity

Human

Applications

WB, ICC, IF, IHC, IP

Purity

ProA affinity purified

Pricing

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ICC (Immunocytochemistry) (ICC staining CDK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

CDK1, Monoclonal Antibody (Cat# AAA30059)

Full Name

CDK1 Antibody

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IF, IHC, IP

Purity

ProA affinity purified

Pricing

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WB (Western Blot) (Human RPMI-8226WB Suggested Anti-KRAS antibody Titration: 1 ug/mLSample Type: Human RPMI-8226)

KRAS, Polyclonal Antibody (Cat# AAA23564)

Full Name

KRAS antibody - N-terminal region

Gene Names

KRAS; NS; NS3; CFC2; RALD; K-Ras; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2

Reactivity

Predicted Species Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish
Tested Species Reactivty: Human , Mouse

Applications

WB

Purity

Affinity Purified

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of 293 cells with CDC45 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

CDC45, Monoclonal Antibody (Cat# AAA30244)

Full Name

CDC45 Antibody

Gene Names

CDC45; CDC45L; CDC45L2; PORC-PI-1

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IF, IHC, FC/FACS

Purity

ProA affinity purified

Pricing

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ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CDC5L mAb. [Lot No. 2136C1a-1])

CDC5L, Monoclonal Antibody (Cat# AAA10605)

Full Name

Mouse monoclonal antibody Anti-Human CDC5L

Gene Names

CDC5L; CDC5; CEF1; PCDC5RP; CDC5-LIKE; dJ319D22.1

Reactivity

Human

Applications

DB, ICC, IP, WB, FC/FACS

Pricing

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PHB, Polyclonal Antibody (Cat# AAA23420)

Full Name

PHB antibody - C-terminal region

Gene Names

PHB; PHB1; HEL-215; HEL-S-54e

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish

Applications

WB, IHC

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse brain using CDK6 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

CDK6, Polyclonal Antibody (Cat# AAA28330)

Full Name

CDK6 Rabbit pAb

Gene Names

CDK6; PLSTIRE

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug CDK4 antibody . Western blot was performed from the immunoprecipitate using CDK4 antibody at a dilition of 1:1000.)

CDK4, Polyclonal Antibody (Cat# AAA28337)

Full Name

CDK4 Rabbit pAb

Gene Names

CDK4; CMM3; PSK-J3

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using BTRC antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

BTRC, Polyclonal Antibody (Cat# AAA28368)

Full Name

BTRC Rabbit pAb

Gene Names

BTRC; FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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WB (Western Blot) (Western blot analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

CDC25C, Monoclonal Antibody (Cat# AAA25347)

Full Name

CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) (HRP)

Gene Names

CDC25C; CDC25; PPP1R60

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

CDC25C, Monoclonal Antibody (Cat# AAA24459)

Full Name

CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) APC

Gene Names

CDC25C; CDC25; PPP1R60

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western blot analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

CDC25C, Monoclonal Antibody (Cat# AAA25051)

Full Name

CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) (FITC)

Gene Names

CDC25C; CDC25; PPP1R60

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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Application Data (Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown)

PDGFB, Polyclonal Antibody (Cat# AAA23582)

Full Name

PDGFB antibody - N-terminal region

Gene Names

PDGFB; SIS; SSV; IBGC5; PDGF2; c-sis; PDGF-2

Reactivity

Cow, Dog, Guinea Pig, Human, Mouse, Rat, Sheep

Applications

WB, IHC

Purity

Affinity Purified

Pricing

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CDk1, Polyclonal Antibody (Cat# AAA29954)

Full Name

CDk1 Antibody

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IHC, FC/FACS

Purity

Peptide affinity purified

Pricing

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WB (Western Blot) (Western blot analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

CDC25C, Monoclonal Antibody (Cat# AAA25640)

Full Name

CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) (PE)

Gene Names

CDC25C; CDC25; PPP1R60

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

CDC25C, Monoclonal Antibody (Cat# AAA24164)

Full Name

CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) (AP)

Gene Names

CDC25C; CDC25; PPP1R60

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse colon using Galectin 3/Galectin 3/LGALS3 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

Galectin 3/LGALS3, Polyclonal Antibody (Cat# AAA28314)

Full Name

Galectin 3/LGALS3 Rabbit pAb

Gene Names

LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Chk1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

Chk1, Monoclonal Antibody (Cat# AAA30108)

Full Name

Chk1 Antibody

Gene Names

CHEK1; CHK1

Reactivity

Human

Applications

WB, ICC, IF, IHC, FC/FACS

Purity

ProA affinity purified

Pricing

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Cyclin D1, Monoclonal Antibody (Cat# AAA28377)

Full Name

[KO Validated] Cyclin D1 Rabbit mAb

Gene Names

CCND1; BCL1; PRAD1; U21B31; D11S287E

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (Immunohistochemical analysis of (AAA28653) on paraffin-embedded Human breastcarcinoma tissue. Tissue was fixed withformaldehyde at room temperature. Heatinduced epitope retrieval was performed byEDTA buffer (pH9. 0). Samples wereincubated with primary antibody(1:100) for 1hour at room temperature. Undiluted CRFAnti-Polyvalent HRP Polymer antibody wasused as the secondary antibody.)

Phospho-CDC25A (S124), Polyclonal Antibody (Cat# AAA28653)

Full Name

Phospho-CDC25A (S124) Antibody

Gene Names

CDC25A; CDC25A2

Reactivity

Human (Predicted Reactivity: Rat)

Applications

WB, EIA, IHC

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with p16INK4A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

p16INK4A, Monoclonal Antibody (Cat# AAA30079)

Full Name

p16INK4A Antibody

Gene Names

CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A

Reactivity

Human

Applications

WB, ICC, IF, IHC, IP, FC/FACS

Purity

ProA affinity purified

Pricing

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 600ug extracts of 293T cells using 3ug NPM1 antibody . Western blot was performed from the immunoprecipitate using NPM1 antibody at a dilition of 1:1000.)

NPM1, Polyclonal Antibody (Cat# AAA28362)

Full Name

NPM1 Rabbit pAb

Gene Names

NPM1; B23; NPM

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

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IHC (Immunohistchemistry) (AAA30925 at 1/100 staining human bone tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

MCM5, Polyclonal Antibody (Cat# AAA30925)

Full Name

MCM5 Antibody

Gene Names

MCM5; CDC46; MGORS8; P1-CDC46

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ICC, EIA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of Cdc20 using anti-Cdc20 antibody (AAA19132).Cdc20 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Cdc20 Antibody (AAA19132) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Cdc20/P55 Cdc, Polyclonal Antibody (Cat# AAA19132)

Full Name

Anti-Cdc20/P55 Cdc Picoband Antibody

Gene Names

CDC20; CDC20A; p55CDC; bA276H19.3

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

IHC, WB

Purity

Immunogen affinity purified

Pricing

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hFANCI, Polyclonal Antibody (Cat# AAA31282)

Full Name

Phospho-hFANCI (Ser565) Antibody

Gene Names

FANCI; KIAA1794

Reactivity

Human, Mouse, Rat

Applications

IHC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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IHC (Immunohistochemistry) (At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

CtBP1/2, Polyclonal Antibody (Cat# AAA31423)

Full Name

Phospho-CtBP1/2 (Ser158/Ser164) Antibody

Gene Names

CTBP1; BARS

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (82%), Bovine (100%), Horse (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

WB, IHC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

MCM2, Polyclonal Antibody (Cat# AAA31328)

Full Name

Phospho-MCM2 (Ser108) Antibody

Gene Names

MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ICC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3225).)

Prohibitin, Monoclonal Antibody (Cat# AAA23933)

Full Name

Prohibitin (Mitochondrial Marker)

Gene Names

PHB; PHB1; HEL-215; HEL-S-54e

Reactivity

Human

Applications

WB, IF, IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

E2F-2, Polyclonal Antibody (Cat# AAA31340)

Full Name

E2F-2 Antibody

Gene Names

E2F2; E2F-2

Reactivity

Human, Mouse
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (90%), Dog (100%)

Applications

WB, IHC, IF, ICC, EIA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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MSH2, Polyclonal Antibody (Cat# AAA31299)

Full Name

Phospho-MSH2 (Tyr238) Antibody

Gene Names

MSH2; FCC1; COCA1; HNPCC; LCFS2; HNPCC1

Reactivity

Human, Mouse, Rat

Applications

IHC, IF, ICC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

MCM2, Polyclonal Antibody (Cat# AAA31329)

Full Name

Phospho-MCM2 (Ser41) Antibody

Gene Names

MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, ICC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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HP1 alpha, Polyclonal Antibody (Cat# AAA31332)

Full Name

Phospho-HP1 alpha (Ser92) Antibody

Gene Names

CBX5; HP1; HP1A

Reactivity

Human, Mouse, Rat

Applications

IHC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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MCM2, Polyclonal Antibody (Cat# AAA31460)

Full Name

Phospho-MCM2 (Ser27) Antibody

Gene Names

MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)

Applications

WB, IHC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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CDC45L, Monoclonal Antibody (Cat# AAA19360)

Full Name

Anti-CDC45L Antibody (monoclonal, 6H6)

Gene Names

CDC45; CDC45L; CDC45L2; PORC-PI-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HEPA1-6 cells using anti-Rad9b antibody (AAA19340).Overlay histogram showing HEPA1-6 cells stained with AAA19340 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rad9b Antibody (AAA19340, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Rad9b, Polyclonal Antibody (Cat# AAA19340)

Full Name

Anti-Rad9b Antibody

Gene Names

Rad9b; BC021784; A630082N15Rik

Reactivity

Mouse, Rat

Applications

WB, IHC-P, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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WB (Western Blot) (Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3194).)

Prohibitin, Monoclonal Antibody (Cat# AAA23932)

Full Name

Prohibitin (Mitochondrial Marker)

Gene Names

PHB; PHB1; HEL-215; HEL-S-54e

Reactivity

Human

Applications

WB, IF, IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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MCM2, Polyclonal Antibody (Cat# AAA31459)

Full Name

Phospho-MCM2 (Ser139) Antibody

Gene Names

MCM2; BM28; CCNL1; CDCL1; cdc19; D3S3194; MITOTIN

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (80%), Bovine (83%), Horse (100%), Rabbit (100%), Dog (100%), Xenopus (100%)

Applications

WB, IHC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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RBL2, Polyclonal Antibody (Cat# AAA31451)

Full Name

Phospho-RBL2 (Thr642) Antibody

Gene Names

RBL2; Rb2; P130

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (89%), Sheep (100%), Rabbit (89%), Dog (100%)

Applications

WB, IHC, IF, ICC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HEPA1-6 cells using anti-Rad9/Rad9a antibody (AAA19288).Overlay histogram showing HEPA1-6 cells stained with AAA19288 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rad9/Rad9a Antibody (AAA19288,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Rad9/Rad9a, Polyclonal Antibody (Cat# AAA19288)

Full Name

Anti-Rad9/Rad9a Antibody

Gene Names

Rad9a; Rad9

Reactivity

Mouse, Rat

Applications

WB, IHC-P, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of A549 cells using anti-MCM4 antibody (AAA19257).Overlay histogram showing A549 cells stained with AAA19257 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MCM4 Antibody (AAA19257, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MCM4, Polyclonal Antibody (Cat# AAA19257)

Full Name

Anti-MCM4 Antibody

Gene Names

MCM4; NKCD; CDC21; CDC54; NKGCD; hCdc21; P1-CDC21

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A549 cells using anti-MCM5 antibody (AAA19377).Overlay histogram showing A549 cells stained with AAA19377 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- MCM5 Antibody (AAA19377, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MCM5, Monoclonal Antibody (Cat# AAA19377)

Full Name

Anti-MCM5 Antibody (monoclonal, 4G10)

Gene Names

MCM5; CDC46; P1-CDC46

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistochemistry) (At 1/200 staining Human prostate cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

CDK4, Polyclonal Antibody (Cat# AAA31380)

Full Name

Phospho-CDK4 (Thr172) Antibody

Gene Names

CDK4; CMM3; PSK-J3

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (85%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (92%)

Applications

WB, IHC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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Bub1, Polyclonal Antibody (Cat# AAA31312)

Full Name

Phospho-Bub1 (Ser459) Antibody

Gene Names

BUB1; BUB1A; BUB1L; hBUB1

Reactivity

Human, Rat

Applications

IHC, IF, ICC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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RAD17, Polyclonal Antibody (Cat# AAA31431)

Full Name

Phospho-RAD17 (Ser646) Antibody

Gene Names

RAD17; CCYC; R24L; RAD24; HRAD17; RAD17SP

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%)

Applications

WB, IHC, IF, ICC, EIA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (Figure 1. Western blot analysis of Ran using anti-Ran antibody (AAA19131). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate,Lane 2: rat testis tissue lysate,Lane 3: rat thymus tissue lysate,Lane 4: mouse brain tissue lysate,Lane 5: mouse testis tissue lysate,Lane 6: mouse thymus tissue lysate,Lane 7: human A549 whole cell lysate,Lane 8: human 22RV1 whole cell lysate,Lane 9: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ran antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ran at approximately 24KD. The expected band size for Ran is at 24KD.)