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8299 results for "Phosphorylation" - showing 1250-1300


product-image-AAA37406_WB15.jpg WB (Western Blot) (Western blot analysis of extracts of A-431 cells, using Phospho-PLCG1-Y783 antibody (AAA37406) at 1:1000 dilution.A-431 cells were treated by EGF (25 ug/ml) at 37°C for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.)

PLCG1-Y783, Antibody (Cat# AAA37406)

Full Name
Phospho-PLCG1-Y783 Polyclonal Antibody
Gene Names
PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
Applications
Western Blot
Purity
Affinity purification
Pricing
product-image-AAA243328_IF11.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using CDK6(phospho-Tyr24) Antibody.)

CDK6, Polyclonal Antibody (Cat# AAA243328)

Full Name
Phospho-CDK6 (Tyr24) Antibody
Gene Names
CDK6; PLSTIRE
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
Pricing
product-image-AAA243100_IF13.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody.)

PAK1/PAK2/PAK3, Polyclonal Antibody (Cat# AAA243100)

Full Name
Phospho-PAK1/PAK2/PAK3 (Thr423/Thr402/Thr421) Antibody
Gene Names
PAK1; PAKalpha
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
Pricing
product-image-AAA316451_WB13.jpg WB (Western Blot) (Western blot analysis of lysates from MDA-MB-435 cells treated with EGF 200ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is blocked with the phospho peptide.)

4E-BP1, Polyclonal Antibody (Cat# AAA316451)

Full Name
4E-BP1 (Phospho-Thr45) Antibody
Gene Names
EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
product-image-AAA109458_WB15.jpg WB (Western Blot) (Western blot analysis of extracts from HepG2 cells treated with Adriamycin (0.5uM, 24hours) and Jurkat cells treated with PMA (125ng/ml, 30mins) using PAK2 (Phospho-Ser141) antibody.)

PAK2, Antibody (Cat# AAA109458)

Full Name
PAK2 (Phospho-Ser141) Antibody
Gene Names
PAK2; PAK65; PAKgamma
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109512_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using ATR (Phospho-Ser428) antibody. ATR (Phospho-Ser428) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

ATR, Antibody (Cat# AAA109512)

Full Name
ATR (Phospho-Ser428) Antibody
Gene Names
ATR; FRP1; MEC1; SCKL; FCTCS; SCKL1
Reactivity
Human
Applications
Immunohistochemistry
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA109741_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using CAD (Phospho-Thr456) antibody. CAD (Phospho-Thr456) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

CAD, Antibody (Cat# AAA109741)

Full Name
CAD (Phospho-Thr456) Antibody
Gene Names
CAD; CDG1Z; GATD4; EIEE50
Reactivity
Human, Mouse
Applications
Immunohistochemistry
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA111973_IF11.jpg IF (Immunofluorescence) (P-peptide-+ Immunofluorescence analysis of NIH/3T3 cells, using Bcr (Phospho-Tyr360) antibody.)

Bcr, Antibody (Cat# AAA111973)

Full Name
Bcr (Phospho-Tyr360) Antibody
Gene Names
BCR; ALL; CML; PHL; BCR1; D22S11; D22S662
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA144355_SDS_PAGE11.jpg SDS-PAGE

Cancer/Testis Antigen 1B, Recombinant Protein (Cat# AAA144355)

Full Name
Recombinant Cancer/Testis Antigen 1B (CTAG1B)
Reactivity
Homo sapiens (Human)
Applications
Western Blot
Purity
> 90%
Pricing
product-image-AAA243358_IF10.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using p53 (Phospho-Ser15) Antibody.)

TP53, Polyclonal Antibody (Cat# AAA243358)

Full Name
Phospho-TP53 (Ser15) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
Pricing
product-image-AAA111030_WB13.jpg WB (Western Blot) (P-Peptide-+ Western blot analysis of extracts from SKOV3 cells using VEGFR2 (phospho-Tyr1214) antibody.)

VEGFR2, Antibody (Cat# AAA111030)

Full Name
VEGFR2 (Phospho-Tyr1214) Antibody
Gene Names
KDR; FLK1; CD309; VEGFR; VEGFR2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA112196_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HeLa cells using Histone H3.1 (phospho-Ser10) antibody.)

Histone H3.1, Antibody (Cat# AAA112196)

Full Name
Histone H3.1 (Phospho-Ser10) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA243371_WB10.jpg WB (Western Blot) (Western blot analysis of extracts from PC12 cells, treated with PMA or calf intestinal phosphatase (CIP), using PKCtheta (Phospho-Ser676) Antibody.)

PRKCQ, Polyclonal Antibody (Cat# AAA243371)

Full Name
Phospho-PRKCQ (Ser676) Antibody
Gene Names
PRKCQ; PRKCT; nPKC-theta
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin
Pricing
product-image-AAA201756_WB13.jpg WB (Western Blot) (25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. The protein is modified by phosphorylation, and a putative 49 kDa isoform also contains this immunizing peptide sequence.)

SOX9, Polyclonal Antibody (Cat# AAA201756)

Full Name
SOX9 antibody - N-terminal region
Gene Names
SOX9; CMD1; SRA1; CMPD1; SRXX2; SRXY10
Reactivity
Tested Species Reactivity: Human, Mouse
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Rabbit, Zebrafish
Applications
Western Blot
Purity
Protein A purified
Pricing
product-image-AAA307623_IF11.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using c-Cbl(phospho-Tyr700) Antibody.)

c-Cbl, Polyclonal Antibody (Cat# AAA307623)

Full Name
c-Cbl (phospho-Tyr700) Antibody
Gene Names
CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA316543_WB13.jpg WB (Western Blot) (Western blot analysis of lysates from MDA-MB-435 cells treated with TNF-alpha, using NF-kappaB p100/p52 (Phospho-Ser869) Antibody. The lane on the left is blocked with the phospho peptide.)

NF-kappaB p100/p52, Polyclonal Antibody (Cat# AAA316543)

Full Name
NF-kappaB p100/p52 (Phospho-Ser869) Antibody
Gene Names
NFKB2; p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100
Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
product-image-AAA144216_AD11.jpg Application Data

Fibrinogen Like Protein 2, Recombinant Protein (Cat# AAA144216)

Full Name
Recombinant Fibrinogen Like Protein 2 (FGL2)
Reactivity
Mouse
Applications
Western Blot
Purity
> 95%
Pricing
product-image-AAA109237_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human heart tissue using PEA-15 (Phospho-Ser104) antibody. Western blot analysis of extracts from COS cells, treated with TNF (20ng/ml, 5mins), using PEA-15 (Phospho-Ser104) antibody.)

PEA-15, Antibody (Cat# AAA109237)

Full Name
PEA-15 (Phospho-Ser104) Antibody
Gene Names
PEA15; PED; MAT1; HMAT1; MAT1H; PEA-15; HUMMAT1H; PED-PEA15; PED/PEA15
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA108975_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HeLa cells treated with PMA (125ng/ml, 30min), using GR (phospho-Ser211) antibody.)

GR, Antibody (Cat# AAA108975)

Full Name
GR (Phospho-Ser211) Antibody
Gene Names
NR3C1; GR; GCR; GRL; GCCR; GCRST
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
product-image-AAA150304_SDS_PAGE11.jpg SDS-PAGE

Casein Alpha, Recombinant Protein (Cat# AAA150304)

Full Name
Recombinant Casein Alpha (CSN1)
Applications
Western Blot
Purity
> 95%
Pricing
product-image-AAA112006_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using TGFBR2 (Ab-250) antibody. Western blot analysis of extracts from NIH/3T3 cells, using TGFBR2 (Ab-250) antibody)

TGF beta Receptor II, Antibody (Cat# AAA112006)

Full Name
TGF beta Receptor II (Ab-250) Antibody
Gene Names
TGFBR2; AAT3; FAA3; LDS2; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TBRII; TBR-ii; TGFR-2; TGFbeta-RII
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA321298_WB13.jpg WB (Western Blot) (Western blot analysis of extracts of HT29 cells, using Phospho-CDC25B(Ser151) Antibody.)

CDC25B, Polyclonal Antibody (Cat# AAA321298)

Full Name
Phospho-CDC25B(Ser151) Antibody
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA145424_SDS_PAGE15.jpg SDS-PAGE

Aminoadipate Semialdehyde Synthase, Recombinant Protein (Cat# AAA145424)

Full Name
Recombinant Aminoadipate Semialdehyde Synthase (AASS)
Reactivity
Homo sapiens (Human)
Applications
Western Blot
Purity
> 85%
Pricing
product-image-AAA321618_IHC11.jpg IHC (Immunohistochemisry) (AAA321618 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

STAT5A, Polyclonal Antibody (Cat# AAA321618)

Full Name
Phospho-STAT5A (Tyr694) Antibody
Gene Names
STAT5A; MGF; STAT5
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification.
Pricing
product-image-AAA143966_SDS_PAGE13.jpg SDS-PAGE

Inter Alpha-Globulin Inhibitor H2, Recombinant Protein (Cat# AAA143966)

Full Name
Recombinant Inter Alpha-Globulin Inhibitor H2 (ITIH2)
Gene Names
ITIH2; H2P; SHAP
Reactivity
Homo sapiens (Human)
Applications
Western Blot
Purity
> 95%
Pricing
product-image-AAA321374_IHC13.jpg IHC (Immunohiostchemistry) (AAA321374 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

PSD95, Polyclonal Antibody (Cat# AAA321374)

Full Name
Phospho-PSD95 (Ser295) Antibody
Gene Names
DLG4; PSD95; SAP90; SAP-90
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA320741_IF11.jpg IF (Immunofluorescence) (AAA320741 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Retinoic Acid Receptor alpha, Polyclonal Antibody (Cat# AAA320741)

Full Name
Phospho-Retinoic Acid Receptor alpha (Ser77) Antibody
Gene Names
RARA; RAR; NR1B1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA321372_IHC13.jpg IHC (Immunohiostchemistry) (AAA321372 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Progesterone Receptor, Polyclonal Antibody (Cat# AAA321372)

Full Name
Phospho-Progesterone Receptor (Ser190) Antibody
Gene Names
PGR; PR; NR3C3
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA321344_WB13.jpg WB (Western Blot) (Western blot analysis of extracts of MOLT-15 cells treated with phosphatase, using Phospho-LCP1 (Tyr28) Antibody.)

LCP1, Polyclonal Antibody (Cat# AAA321344)

Full Name
Phospho-LCP1 (Tyr28) Antibody
Gene Names
LCP1; LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA321631_WB13.jpg WB (Western Blot) (Western blot analysis of p27 Kip1 phosphorylation expression in HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

p27 Kip1, Polyclonal Antibody (Cat# AAA321631)

Full Name
Phospho-p27 Kip1 (Thr198) Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA321736_IF8.jpg IF (Immunofluorescence) (AAA321736 staining HeLa cells treated with TNF-a 20nM 15' by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

I kappaB beta, Polyclonal Antibody (Cat# AAA321736)

Full Name
Phospho-I kappaB beta (Ser23) Antibody
Gene Names
NFKBIB; IKBB; TRIP9
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA321428_WB10.jpg WB (Western Blot) (Western blot analysis of IKK-beta phosphorylation expression in COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IKK beta, Polyclonal Antibody (Cat# AAA321428)

Full Name
Phospho-IKK beta (Tyr188) Antibody
Gene Names
IKBKB; IKK2; IKKB; IMD15; NFKBIKB; IKK-beta
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA47013_WB13.jpg WB (Western Blot) (Western blot analysis of p16 Arc expression in (1)HeLa cell lysate; (2)HepG2 cell lysate (AAA47013).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRKAA1)

AMPK alpha 1, Monoclonal Antibody (Cat# AAA47013)

Full Name
Anti-AMPK alpha 1 Rabbit Monoclonal Antibody
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry
Purity
Affinity-chromatography
Pricing
product-image-AAA124537_WB13.jpg WB (Western Blot) (Western blot analysis of Phospho-PAK1/2/3 expression in HeLa Cell lysate treated with lambda phosphatase (AAA124537).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PAK3)

PAK1/2/3, Monoclonal Antibody (Cat# AAA124537)

Full Name
Anti-Phospho-PAK1/2/3 (S144+S141+S139) Rabbit Monoclonal Antibody
Gene Names
PAK3; ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry
Purity
Affinity-chromatography
Pricing
product-image-AAA321732_WB10.jpg WB (Western Blot) (Western blot analysis of Phospho-Caspase 8 (Ser347) expression in various lysates)

Caspase 8, Polyclonal Antibody (Cat# AAA321732)

Full Name
Phospho-Caspase 8 (Ser347) Antibody
Gene Names
CASP8; CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA289063_IHC10.jpg IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

FGFR4, Polyclonal Antibody (Cat# AAA289063)

Full Name
FGFR4 Antibody (N-term)
Gene Names
FGFR4; TKF; JTK2; CD334
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA318647_IHC10.png IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4 degree overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

p70 S6 kinase alpha, Polyclonal Antibody (Cat# AAA318647)

Full Name
p70 S6 kinase alpha (Phospho-Thr421) Rabbit Polyclonal Antibody
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA283718_IHC13.jpg IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Phospho-p27Kip1 (T187), Polyclonal Antibody (Cat# AAA283718)

Full Name
Phospho-p27Kip1 (T187) Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human (Predicted Reactivity: Hamster)
Applications
Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA37354_WB15.jpg WB (Western Blot) (Western blot analysis of extracts from 3T3 cells, using Phospho-IRS1-S636 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)

IRS1-S636, Antibody (Cat# AAA37354)

Full Name
Phospho-IRS1-S636 Polyclonal Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse.
Applications
Western Blot
Purity
Affinity Purification
Pricing
product-image-AAA37370_IF11.jpg IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-LIMK2-T505 antibody.)

LIMK2-T505, Antibody (Cat# AAA37370)

Full Name
Phospho-LIMK2-T505 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Pricing
product-image-AAA236199_WB13.jpg WB (Western Blot) (Western Blot analysis of Jurkat cells using GSK3alpha Polyclonal Antibody)

GSK3A, Polyclonal Antibody (Cat# AAA236199)

Full Name
GSK3A Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA172265_IF11.jpg IF (Immunofluorescence) (Immunofluorescence analysis of Rat testis tissue with Phospho-JNK1/2/3 (Thr183) Polyclonal Antibody at dilution of 1:200)

JNK1/2/3, Polyclonal Antibody (Cat# AAA172265)

Full Name
Phospho-JNK1/2/3 (T183) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Pricing
product-image-AAA215947_FCM15.jpg FCM/FACS (Flow Cytometry) (Flow cytometric analysis of PFA-fixed HeLa cells. STAT5A Mouse Monoclonal Antibody (PCRP-STAT5A-1A11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).)

STAT5A, Monoclonal Antibody (Cat# AAA215947)

Full Name
STAT5A Mouse Monoclonal Antibody [Clone PCRP-STAT5A-1A11]
Gene Names
STAT5A; MGF; STAT5
Reactivity
Human
Predicted to work with Mouse and Rat.
Applications
Flow Cytometry
Pricing
product-image-AAA236345_WB13.jpg WB (Western Blot) (Western Blot analysis of Jurkat K562 cells using NFkappaB-p105 Polyclonal Antibody)

NFKB1, Polyclonal Antibody (Cat# AAA236345)

Full Name
NFKB1 Antibody
Gene Names
NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA235861_WB13.jpg WB (Western Blot) (Western Blot analysis of A549 cells using Phospho-PKC delta (S645) Polyclonal Antibody)

PRKCD, Polyclonal Antibody (Cat# AAA235861)

Full Name
Phospho-PRKCD (S645) Antibody
Gene Names
PRKCD; MAY1; PKCD; nPKC-delta
Reactivity
Human, Monkey
Applications
Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA316516_WB13.jpg WB (Western Blot) (Western blot analysis of lysates from HepG2 cells, using IRS-1 (Phospho-Ser312) Antibody. The lane on the right is blocked with the phospho peptide.)

IRS-1, Polyclonal Antibody (Cat# AAA316516)

Full Name
IRS-1 (Phospho-Ser312) Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
product-image-AAA318322_WB13.jpg WB (Western Blot) (Western blot analysis of lysates from LOVO cells, using HDAC2 (Phospho-Ser394) Antibody.)

HDAC2, Polyclonal Antibody (Cat# AAA318322)

Full Name
HDAC2 (Phospho-Ser394) Antibody
Gene Names
HDAC2; HD2; RPD3; YAF1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
product-image-AAA110480_AD15.png Application Data (P-peptide- + ++Western blot analysis of extracts from HeLa and JK, using Paxillin (Phospho-Ser272) Antibody.)

Paxillin, Antibody (Cat# AAA110480)

Full Name
Paxillin (Phospho-Ser272) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide.
Pricing
product-image-AAA242228_IF13.jpg IF (Immunofluorescence) (Immunofluorescence analysis of LOVO cells, using Bax (Ab-167) antibody.)

BAX, Polyclonal Antibody (Cat# AAA242228)

Full Name
BAX (Ab-167) Antibody
Gene Names
BAX; BCL2L4
Reactivity
Human, Mouse
Applications
Immunofluorescence, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA307919_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from HT29 cells, treated with PMA or calf intestinal phosphatase (CIP), using Cdc25B (Phospho-Ser149) Antibody.)

Cdc25B, Polyclonal Antibody (Cat# AAA307919)

Full Name
Cdc25B (Phospho-Ser149) Antibody
Gene Names
Cdc25b; AI604853
Reactivity
Mouse
Applications
Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Pricing

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