Mouse E-Cadherin/CD324 Monoclonal Antibody | anti-CD324 antibody

E-Cadherin/CD324 (Intercellular Junction Marker)

Cat. Num. AAA23896

• Gene Names: CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
• Reactivity: Human.; Does not react with Mouse and Rat. Others not known.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Synonyms: E-Cadherin/CD324, Antibody; E-Cadherin/CD324 (Intercellular Junction Marker); Arc 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD324; CDH1; CDHE; E-Cad/CTF3; E-cadherin; ECAD; Epithelial cadherin; epithelial calcium dependent adhesion protein; Liver cell adhesion molecule (LCAM); Uvomorulin (UVO); anti-CD324 antibody

• Host: Mouse
• Reactivity: Human.; Does not react with Mouse and Rat. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: CDH1/1525
• Specificity: Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as beta-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help of mesothelioma.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 821

• Applicable Applications for anti-CD324 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: FC/FACS: 0.5-1ug/million cells; IF: 0.5-1ug/ml; WB: 0.5-1ug/ml; IHC-F: 1-2ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Immunogen: Recombinant human full-length E-Cadherin protein
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 16q22.1
• Positive Control: LS174T, Raji, HT29, SK-BR3 cells. Prostate or Colon carcinomas.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohistchemistry)

(Formalin-paraffin human Placenta stained with E-Cadherin MAb (CDH1/1525).)

IHC (Immunohistochemistry)

(Formalin-paraffin human Prostate Carcinoma stained with E-Cadherin MAb (CDH1/1525).)

IHC (Immunohistochemistry)

(Formalin-paraffin human Colon Carcinoma stained with E-Cadherin MAb (CDH1/1525).)

WB (Western Blot)

(Western Blot Analysis (A) Recombinant Protein (B) human Stomach lysate Using E-Cadherin Monoclonal Antibody (CDH1/1525).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Skin stained with E-Cadherin Monoclonal Antibody (CDH1/1525).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with E-Cadherin Monoclonal Antibody (CDH1/1525).)

References

Umbas, R., et al. 1992. Expression of the cellular adhesion molecule E-cadherin is reduced or absent in high-grade prostate cancer. Cancer Res. 52: 5104-5109.

NCBI and Uniprot Product Information

• NCBI GI #: 953768348
• NCBI GeneID: 999
• NCBI Accession #: NP_001304113.1
• NCBI GenBank Nucleotide #: NM_001317184.1
• UniProt Accession #: P12830; Q13799; Q14216; Q15855; Q16194; Q4PJ14; Q9UII8; A8K1U7
• Molecular Weight: 120-80kDa (Mature); 135kDa (Precursor)
• NCBI Official Full Name: cadherin-1 isoform 2
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: CDH1
• NCBI Official Synonym Symbols: UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
• NCBI Protein Information: cadherin-1
• UniProt Protein Name: Cadherin-1
• UniProt Gene Name: CDH1
• UniProt Synonym Gene Names: CDHE; UVO; E-cadherin