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Application Data (Anti-CD20 Reference Antibody (rituximab)-induced ADCC activity was evaluated using Raji Cell. The max Lysis rate was approximately 35%.)

CD20 recombinant antibody

Anti-CD20 Reference Antibody (rituximab)

Gene Names
MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
Reactivity
Human, Cynomolgus
Applications
ELISA, Flow Cytometry, Functional Assay, Functional Assay
Synonyms
CD20; Monoclonal Recombinant Antibody; Anti-CD20 Reference Antibody (rituximab); CD20 recombinant antibody
Ordering
For Research Use Only!
Host
CHO Cell
Reactivity
Human, Cynomolgus
Clonality
Monoclonal
Isotype
IgG1
Specificity
CD20
Form/Format
Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.
Concentration
1mg/ml (varies by lot)
Applicable Applications for CD20 recombinant antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Kinetics, Functional Assay (FUNC), Animal Model
Endotoxin
< 0.001EU/ug, determined by LAL method.
Conjugation
Unconjugated
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store at -80 degree C for 2 years under sterile conditions; -20 degree C for 1 year under sterile conditions; Avoid repeated freeze-thaw cycles.

Application Data

(Anti-CD20 Reference Antibody (rituximab)-induced ADCC activity was evaluated using Raji Cell. The max Lysis rate was approximately 35%.)

Application Data (Anti-CD20 Reference Antibody (rituximab)-induced ADCC activity was evaluated using Raji Cell. The max Lysis rate was approximately 35%.)

Application Data

(Anti-CD20 Reference Antibody (rituximab)-induced CDC activity was evaluated using Raji Cell. The max Lysis rate was approximately 74%.)

Application Data (Anti-CD20 Reference Antibody (rituximab)-induced CDC activity was evaluated using Raji Cell. The max Lysis rate was approximately 74%.)

FCM (Flow Cytometry)

(Raji cells were stained with Anti-CD20 Reference Antibody (rituximab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC87=1.334 ug/mL)

FCM (Flow Cytometry) (Raji cells were stained with Anti-CD20 Reference Antibody (rituximab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC87=1.334 ug/mL)

Application Data

(Immobilized human EGFR His at 2 ug/mL can bind Anti-CD20 Reference Antibody (rituximab), EC50=0.01573/0.01258ug/mL)

Application Data (Immobilized human EGFR His at 2 ug/mL can bind Anti-CD20 Reference Antibody (rituximab), EC50=0.01573/0.01258ug/mL)

Application Data

(The purity of Anti-CD20 Reference Antibody (rituximab)is more than 98.97% ,determined by SEC-HPLC.)

Application Data (The purity of Anti-CD20 Reference Antibody (rituximab)is more than 98.97% ,determined by SEC-HPLC.)

SDS-PAGE

(Anti-CD20 Reference Antibody (rituximab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%)

SDS-PAGE (Anti-CD20 Reference Antibody (rituximab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%)
Product Categories/Family for CD20 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
931
UniProt Accession #
Molecular Weight
297
NCBI Official Full Name
B-lymphocyte antigen CD20
NCBI Official Synonym Full Names
membrane-spanning 4-domains, subfamily A, member 1
NCBI Official Symbol
MS4A1
NCBI Official Synonym Symbols
B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
NCBI Protein Information
B-lymphocyte antigen CD20; CD20 antigen; CD20 receptor; leukocyte surface antigen Leu-16; B-lymphocyte cell-surface antigen B1
UniProt Protein Name
B-lymphocyte antigen CD20
UniProt Gene Name
MS4A1
UniProt Synonym Gene Names
CD20
UniProt Entry Name
CD20_HUMAN

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Product Notes

The CD20 ms4a1 (Catalog #AAA29393) is a Recombinant Antibody produced from CHO Cell and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD20 Reference Antibody (rituximab) reacts with Human, Cynomolgus and may cross-react with other species as described in the data sheet. AAA Biotech's CD20 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Kinetics, Functional Assay (FUNC), Animal Model. Researchers should empirically determine the suitability of the CD20 ms4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD20, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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