Antibody Sandwich ELISA Development Kit Accessory Reagent
Antibody Sandwich ELISA Development Kit
Synonyms
Antibody Sandwich ELISA Development Kit; N/A; Antibody-Sandwich ELISA Development Kit; Antibody Sandwich ELISA Development Kit accessory reagent
Assay Type
Sandwich
Related Product Information for Antibody Sandwich ELISA Development Kit accessory reagent
Background: The Antibody-Sandwich (AS) format ELISA may be the most useful of the immunosorbent assays for detecting antigen because it is both sensitive and robust. An Antibody-Sandwich format ELISA refers to an ELISA plate configuration whereby the analyte being quantified is bound, or "sandwiched" between two layers of antibodies (i.e. capture and detection antibody). In this ELISA format, the plate wells are coated with a monoclonal or affinity purified polyclonal antibody ("capture" antibody). Following the antibody coating step, plate wells are then washed and incubated with a blocking buffer to block the uncoated regions and stabilize the coated antibody. Next, in the capture incubation step, the coated antibody captures target analyte present in samples (see Figure 1). Afterwards, the plate is washed and bound antigen is incubated with the "up" antibody which is used to quantify the amount or concentration of target analyte in the samples. The up antibody may be a monoclonal or affinity purified polyclonal, and it may be directly labeled with enzyme used for detection, such as HRP (in this case, the up antibody and detection antibody are the same). Alternatively, HRP labeled secondary antibody (detection antibody) specific for the unlabeled up antibody may be used in a subsequent incubation step. After the incubation period has ended, ELISA plate wells are washed to remove all non-specifically bound HRP conjugate (detection antibody). The next step is to add a chromogenic HRP substrate, such as TMB, to the plate wells. TMB substrate will allow for the visualization and electronic quantitation of how much target analyte was present within the samples. In general, the more intense the TMB color present within a respective sample-well, the greater the amount of analyte present. To summarize, the basic AS ELISA format is capable of quantifying the amount or concentration of target analyte present within test samples. This Antibody-Sandwich ELISA Development Kit was created to help guide end users through the common steps and frequently encountered issues associated with building an AS ELISA. The guide describes assay performance milestones that must be achieved to enable progression through the various development stages of the analyte-specific sandwich format ELISA.
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Product Notes
The Antibody Sandwich ELISA Development Kit (Catalog #AAA52751) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Antibody Sandwich ELISA Development Kit, Accessory Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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