Antigen-Down ELISA Development Kit Accessory Reagent
Antigen-Down ELISA Development Kit
Synonyms
Antigen-Down ELISA Development Kit; N/A; Antigen-Down ELISA Development Kit accessory reagent
Related Product Information for Antigen-Down ELISA Development Kit accessory reagent
Background: An Antigen-Down (AD) format ELISA refers to an ELISA plate configuration whereby the plate wells are pre-coated with a highly purified target antigen (hereafter called Target Antigen) that will subsequently be used to quantify specific antibody concentrations that are present within serological or cell culture origin samples (Figure 1). Having the ability to detect and quantitate a population of specific antibodies to a particular pathogen, allergen, or selfantigen within serological samples is essential for any successful epidemiological, allergic hypersensitivity, or autoimmune focused monitoring study. Briefly, the specific antibody detection and quantification attributes of the AD ELISA format result from its simplistic design. Commercial ELISA plate manufacturers each utilize their own inhouse polystyrene plastics modification process. Inner surfaces of each sample well possess the modified plastics surface chemistry to enable passive binding of the Target Antigen to the individual plate well surfaces. Examples of different types of Target Antigens include oligopeptides, polypeptides, and certain polysaccharide molecules. After the Target Antigen has been adsorbed to the ELISA plate wells, the entire well surface is treated with a blocking agent that will minimize unwanted non-specific binding signal. Reduction of nonspecific binding signal is a universal goal of all types of ELISA format. Once the Target Antigen-coated plate has been created (blocked and stabilized), screening of serum, plasma, or cell culture samples for the presence of a Target Antigen-specific poly or monoclonal antibody concentration or titer can begin. To do so, samples are incubated in the coated wells, after which the ELISA plates are washed to remove unbound sample. Next, anti-animal isotype specific IgG-HRP conjugate is added to each well and incubated. After the conjugate incubation period has ended, ELISA plate wells are washed to remove all non-specifically bound HRP conjugate. The next step is to add a chromogenic HRP substrate, such as TMB, to the plate wells. This will allow for the visualization and electronic quantitation of how much Target Antigen-specific antibodies were present within the samples. In the presence of the HRP-IgG conjugate (which is specifically bound to Target Antigen that was previously absorbed onto plate well surfaces), the horseradish peroxidase (HRP) will cause a color change in the initially clear TMB substrate solution. In general, the more intense the TMB color present within a respective sample well, the greater the amount of Target Antigen-specific antibody in that particular sample. To summarize, the basic AD ELISA format is capable of providing a relative estimation of the Target Antigen-specific antibody titer/ concentration present within the serological test samples, as well as a means by which to determine the identity of the isotype(s) that are participating in the humoral immune response. This Antigen- Down ELISA Development Kit was created to help guide end users through the common steps and frequently encountered issues associated with building an AD ELISA. The guide describes the assay performance milestones that must be achieved to enable progression through the various development stages of the antigendown format ELISA (Table 1). The finalized ELISA should efficiently detect isotype-specific, low to high positive titer, humoral antibody responses in serum or plasma samples.
Product Categories/Family for Antigen-Down ELISA Development Kit accessory reagent
Similar Products
Product Notes
The Antigen-Down ELISA Development Kit (Catalog #AAA52752) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Antigen-Down ELISA Development Kit, Accessory Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.