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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 2350-2400 of 2567 product results
Bioactivity
(Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 H2O = HCO3- H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. The activity of recombinant human CA2 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA2 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)
Carbonic Anhydrase II (CA2), Active Protein (Cat# AAA161776)
Full Name
Active Carbonic Anhydrase II (CA2)
Gene Names
CA2; CAC; CAII; Car2; CA-II; HEL-76
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a beta-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM). The activity of recombinant chicken RBP4 was measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. RBP4 was diluted to 50 ug/ml in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (assay buffer) and the retinoic acid was diluted to 800, 400, 200, 100, 30, 10, 3, 1, 0.5 and 0.1 uM in 95% ethanol. Mixing 112.5 uL of 50 ug/mL rgRBP4 and 12.5 uL of retinoic acid serial dilutions in microtubes and a blank containing 112.5 uL of 50 ug/mL rgRBP4 and 12.5 uL of 95% ethanol, then incubate at room temperature for 30 minutes. Loading 100 ul of the reaction mixtures and blank and read at excitation and emission wavelengths of 280 nm and 340 nm (top read), respectively, in endpoint mode. The result was shown in figure 1, the 50% binding concentration (BC50) is >50 uM.)
Retinol Binding Protein 4 (RBP4), Active Protein (Cat# AAA161804)
Full Name
Active Retinol Binding Protein 4 (RBP4)
Gene Names
RBP4A; RBP; RBP4
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Collagen alpha-1(VI) chain (COL6a1) is a protein that in humans, the collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Besides, Integrin Alpha V (ITGaV) has been identified as an interactor of COL6A1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human COL6a1 and recombinant human ITGaV. Briefly, COL6a1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to ITGaV -coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-COL6a1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human COL6a1 and recombinant human ITGaV was shown in Figure 1, the EC50 for this effect is 0.19 ug/mL.)
Collagen Type VI Alpha 1 (COL6a1), Active Protein (Cat# AAA161878)
Full Name
Active Collagen Type VI Alpha 1 (COL6a1)
Gene Names
COL6A1; OPLL
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
p300, Active Protein (Cat# AAA77049)
Full Name
p300 (catalytic domain) (human), (recombinant)
Gene Names
EP300; p300; KAT3B; RSTS2
Purity
Partially purified by single-step affinity chromatography and gel filtration
Pricing
Bioactivity
(Maltose binding protein (MBP) encoded by the malE gene in Escherichia coli, is a 44-kD monomeric periplasmic protein. MBP is component of the Escherichia coli maltose/maltodextrin system, which regulates the uptake and catabolism of maltrodextrins as part of the chemotactic response. This protein is used in recombinant protein expression as an affinity and solubility tag. Glutathione S Transferase Alpha 3 (GSTa3) has been identified as an interactor of MBP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant MBP and recombinant mouse GSTa3. Briefly, biotin-linked MBP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to GSTa3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant MBP and recombinant mouse GSTa3 was shown in Figure 1, the EC50 for this effect is 0.46 ug/mL.)
Maltose Binding Protein (MBP), Active Protein (Cat# AAA161942)
Full Name
Active Maltose Binding Protein (MBP)
Gene Names
malE; ECK4026; JW3994; malJ
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(E-Cadherin, also known as Uvomorulin in the mouse and rat, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion. Human E-Cadherin shares 81% amino acid sequence identity with the rat and mouse proteins. It is a single-pass transmembrane protein that mediates calcium-dependent epithelial cell adhesion. As E-cadherin has the function of cell adhesion, we measure the activity of recombinant human E-cadherin by the ability of the immobilized protein to support the adhesion of MCF7 human breast cancer cells. When 5 x 104 cells/well are added to different concentrations of recombinat human E-cadherin coated plates, cells will adhere after 2 hour incubation at 37 degree C. The adhesion of MCF7 after 2 hour incubation at 37 degree C observed by inverted microscope was shown in Figure 1. Cell adherent was in a dose dependent manner, the result was shown in Figure 2, the EC50 was 0.23 ug/ml.)
E-cadherin, Active Protein (Cat# AAA161657)
Full Name
Active E-cadherin
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Application Data
Annexin A1, Active Protein (Cat# AAA150136)
Full Name
Active Annexin A1 (ANXA1)
Gene Names
ANXA1; ANX1; LPC1
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>98%
Pricing
WB (Western Blot)
(Figure 3. Western BlotSample: Recombinant SOD3, Mouse;Antibody: Rabbit Anti-Mouse SOD3 Ab)
Superoxide Dismutase 3, Extracellular, Active Protein (Cat# AAA150073)
Full Name
Active Superoxide Dismutase 3, Extracellular (SOD3)
Gene Names
Sod3; EC-SOD; AI314465
Reactivity
Mus musculus (Mouse)
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Connective Tissue Growth Factor (CTGF), also known as CCN2 is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins. CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. As CTGF has the function of cell adhesion, we measure the activity of recombinant human CTGF by the ability of the immobilized protein to support the adhesion of Balb/3T3 mouse embryonic fibroblast cell. When 5 x 104 cells/well are added to different concentrations of recombinat human CTGF coated plates, cells will adhere after 2 hour incubation at 37 degree C. The adhesion of Balb/3T3 after 2 hour incubation at 37 degree C observed by inverted microscope was shown in Figure 1. Cell adherent was in a dose dependent manner, the result was shown in Figure 2, the EC50 was 0.6 ug/ml.)
Connective Tissue Growth Factor (CTGF), Active Protein (Cat# AAA161649)
Full Name
Active Connective Tissue Growth Factor (CTGF)
Gene Names
CTGF; CCN2; NOV2; HCS24; IGFBP8
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Caspase-5, Active Protein (Cat# AAA77045)
Full Name
Caspase-5 (human), (recombinant)
Gene Names
CASP5; ICH-3; ICEREL-III; ICE(rel)III
Purity
>80% (SDS-PAGE)
Pricing
Application Data
COVID 19 Spike RBD Coronavirus, Active Protein (Cat# AAA268892)
Full Name
SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, mFc Tag)
Purity
>95% as determined by SDS-PAGE.
95% as determined by SEC-HPLC
95% as determined by SEC-HPLC
Pricing
Creatine Kinase MB (CK-MB) Type II, Recombinant, Active Protein (Cat# AAA58330)
Full Name
CK-MB Type II, Recombinant
Purity
SDS-PAGE followed by Coomassie staining must demostrate a doublet band migrating at ~47 kDa.
Testing Result: Pass
Testing Result: Pass
Pricing
Bioactivity
(C-reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q. Besides, Ficolin 2 (FCN2) has been identified as an interactor of CRP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CRP and recombinant mouse FCN2. Briefly, CRP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FCN2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CRP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human CRP and recombinant mouse FCN2 was shown in Figure 1, the EC50 for this effect is 0.008 ug/mL.)
C ReProtein (CRP), Active Protein (Cat# AAA161782)
Full Name
Active C Reactive Protein (CRP)
Gene Names
CRP; PTX1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
eNOS, Active Protein (Cat# AAA77034)
Full Name
eNOS (human), (recombinant)
Gene Names
NOS3; eNOS; ECNOS
Purity
>90% (SDS-PAGE, Western Blot)
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)-His, the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)-His is 10-60 ng/mL.)
COVID 19 Spike S1(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)-His Coronavirus, Active Protein (Cat# AAA258088)
Full Name
SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, Y144 deletion, N501Y, A570D, D614G, P681H)-His Recombinant Protein
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse ST2-His at 10ug/mL (100uL/well) can bind human TMED1-Fc, the EC50 of human TMED1-Fc is 0.05-0.2ug/mL.)
TMED1, Active Protein (Cat# AAA258019)
Full Name
TMED1 Protein, Human, Recombinant (hFc Tag)
Gene Names
TMED1; Tp24; Il1rl1l; IL1RL1LG
Purity
>90% as determined by SDS-PAGE
Pricing
Bioactivity
(Antithrombin III, also known as Serpin C1, is a member of the Serpin superfamily of the serine protease inhibitors. It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa. Similar to Serpins A5 and D1, its thrombin inhibitory activity is enhanced by heparin. Hereditary and acquired Serpin C1 deficiency is the cause of an increased thrombotic tendency in many cases. For example, acquired Serpin C1 deficiency is a common condition in sepsis, after major trauma or surgery. The activity of recombinant rat antithrombin III was measured by its ability to inhibit thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Thrombin was diluted to 0.5 U with heparin at 50 ug/ml in the assay buffer and 10 ul different concentrations of recombinant rat antithrombin III (MW: 52.6 KD) was incubated with 10 ul diluted thrombin at 37 degree C for 30 minutes. Loading 50 uL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 uL of 200 uM substrate. Include a substrate blank containing 50 uL of assay buffer and 50 uL of 200 uM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant rat antithrombin III significantly decreased thrombin activity. The inhibition IC50 was )
Antithrombin (AT), Active Protein (Cat# AAA161887)
Full Name
Active Antithrombin (AT)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
alpha Amylase enzyme, Active Protein (Cat# AAA75393)
Full Name
alpha Amylase enzyme
Bioactivity
(Angiogenin (ANG) was initially purified from serum-free media conditioned by growth of a human adenocarcinoma cell line HT-29 based on its ability to initiate vascularization in the chicken embryo chorioallantoic membrane. A number of other tumor, as well as normal, cell lines can also secrete Angiogenin. In addition, Angiogenin is present in normal human plasma at levels as high as 60-120 ng/mL. Surprisingly, Angiogenin has been found to be a member of the ribonuclease superfamily with approximately 35% sequence similarity at the amino acid level with pancreatic RNase. Angiogenin exhibits ribonucleolytic activity that is distinctly different than that of pancreatic RNase A. The ribonucleolytic activity of Angiogenin toward most RNase A substrates is much lower than that of RNase A. Nevertheless, the ribonucleolytic activity of Angiogenin is essential to its angiogenic activity since inhibition of the Angiogenin RNase activity will also abolish angiogenesis activity. The activity of recombinant mouse ANG was measured by its ribonucleolytic activity toward RNA. The result was shown in figure 1, and the rmANG produces a delta Abs260/ug >1.0 in two hours.)
Angiogenin (ANG), Active Protein (Cat# AAA161648)
Full Name
Active Angiogenin (ANG)
Gene Names
Ang; Ang1; Rnase5; Rnase5a; AI385586
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Growth Hormone, Active Protein (Cat# AAA38548)
Full Name
Recombinant Porcine Growth Hormone
Gene Names
GH1; GH
Purity
Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
Application Data
(Measured by its ability to promote proliferation of INS-1 cells. The ED50 for this effect is 0.2-1 ng/mL.)
Growth Hormone, Active Protein (Cat# AAA258048)
Full Name
Growth Hormone Protein, Human, Recombinant
Gene Names
GH1; GH; GHN; GH-N; hGH-N; IGHD1B
Purity
>90% as determined by SDS-PAGE
Pricing
HDAC2, Active Protein (Cat# AAA77050)
Full Name
HDAC2 (full-length) (human), (recombinant)
Purity
Partially purified by single-step affinity chromatography and gel filtration
Pricing
HIV1 gp120, Active Protein (Cat# AAA75444)
Full Name
HIV1 gp120 protein
Purity
> 95% by SDS-PAGE
Pricing
STREPTOLYSIN-O, Active Protein (Cat# AAA50417)
Full Name
RECOMBINANT STREPTOLYSIN-O
Applications
ELISA
Pricing
PKC-delta, Active Protein (Cat# AAA71848)
Full Name
PKC-delta Standard
Gene Names
PRKCD; MAY1; PKCD; ALPS3; CVID9; nPKC-delta
Applications
Western Blot
Pricing
Complement C3, Active Protein (Cat# AAA75344)
Full Name
Complement C3 protein
Gene Names
C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
Purity
>95% by SDS-PAGE
Pricing
Complement C5b, 6 complex ag, Active Protein (Cat# AAA75367)
Full Name
Complement C5b, 6 complex protein
Reactivity
Human
Purity
>=98% by SDS-PAGE
Pricing
HSV2 gG2, Active Protein (Cat# AAA75436)
Full Name
HSV2 gG2 protein
Applications
Western Blot
Purity
> 95% pure
Pricing
SDS-PAGE
(The purity of KRAS was determined to be >90% by densitometry. Approx. MW 23kDa.)
Purity Data
(Sample Purity Data.)
Application Data
(Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 20-80 ng/mL in the presence of 10 ng/mL Recombinant Human Activin A.)
Follistatin, Active Protein (Cat# AAA258012)
Full Name
Follistatin Protein, Human, Recombinant (His Tag)
Gene Names
FST; FS
Purity
>90% as determined by SDS-PAGE
Pricing
Creatine Kinase BB (CK-BB) Recombinant, Active Protein (Cat# AAA58337)
Full Name
CK-BB, Recombinant
Gene Names
CKB; B-CK; CKBB
Applications
ELISA
Purity
>95% pure (SDS-PAGE). Purified under non-denaturing conditions.
Pricing
Rheumatoid Factor (RF), Active Protein (Cat# AAA44876)
Full Name
Rheumatoid Factor (RF) Concentrate - > 5 kIU/mL
Purity
Purified
Purity Data
(Sample Purity Data.)
COVID 19 NSP14 Methyltransferase Coronavirus, Active Protein (Cat# AAA73169)
Full Name
2019-nCoV NSP14 Methyltransferase, Active
Pricing
ELASTASE, Active Protein (Cat# AAA50496)
Full Name
NATIVE HUMAN ELASTASE (NEUTROPHIL)
Gene Names
ELANE; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
Applications
Functional Assay
Purity
>95% by SDS PAGE
Pricing
GST T1-1, Active Protein (Cat# AAA71852)
Full Name
GST T1-1, Recombinant Human
Purity
>= 95% as assessed by inspection on a Coomassie Blue-stained SDS-PAGE gel.
Pricing
Chymase, Active Protein (Cat# AAA75403)
Full Name
Chymase reagent
Gene Names
CMA1; CYH; MCT1; chymase
Purity
> 95% by SDS-PAGE and gel diffusion
Pricing
Interferon Tau, Active Protein (Cat# AAA75447)
Full Name
Purified recombinant Bovine IFN-Tau protein (Bovine)
Reactivity
Bovine
Purity
>=95% pure
Pricing
Rheumatoid Factor (RF), Active Protein (Cat# AAA44877)
Full Name
Rheumatoid Factor (RF) Concentrate - > 25 kIU/mL
Purity
Purified
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
GSTA1, Active Protein (Cat# AAA48584)
Full Name
GSTA1, 1-222aa, Human, E Coli (Bioactivity Validated)
Gene Names
GSTA1; GST2; GTH1; GSTA1-1; GST-epsilon
Applications
Enzyme Activity, SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
Granzyme A, Active Protein (Cat# AAA77033)
Full Name
Granzyme A (human), (recombinant)
Gene Names
GZMA; HFSP; CTLA3
Purity
>98% (SDS-PAGE)
Pricing
SDS-PAGE
COVID 19 Spike S1 Protein Coronavirus, Active Protein (Cat# AAA176996)
Full Name
Recombinant 2019-nCoV S1 Protein (mFc Tag)
Purity
>95% as determined by reducing SDS-PAGE.
Pricing
MMP-14, Active Protein (Cat# AAA77047)
Full Name
MMP-14 (catalytic domain) (human), (recombinant)
Purity
Partially purified by single-step affinity chromatography and gel filtration
Pricing
Application Data
(The purity was determined to be > 95% by densitometry.)
PDE4A, Active Protein (Cat# AAA73103)
Full Name
PDE4A, Active
Gene Names
PDE4A; PDE4; DPDE2; PDE46
Purity
>95%
Pricing
Bioactivity
(HTRF Assay for Recombinant PDGFRB (557-1106 activity. 1 uM TK substrate was incubated with different concentrations of PDGFRB (557-1106) protein in 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM MnCl, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature.)
PDGFRB, Active Protein (Cat# AAA60333)
Full Name
Recombinant PDGFRB (557-1106) protein
Purity
>50%
Pricing
MMP-7, Active Protein (Cat# AAA77046)
Full Name
MMP-7 (catalytic domain) (human), (recombinant)
Purity
> 95% (SDS-PAGE)
Purified by multi-step chromatography
Purified by multi-step chromatography
Pricing
IFN alpha 2, Active Protein (Cat# AAA75395)
Full Name
IFN alpha 2 protein (Monkey)
Gene Names
IFNA2; IFNA; INFA2; IFNA2B; IFN-alphaA
Purity
> 98% pure
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids. They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their recombinant proteins or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it will typically display high fidelity characteristics to its original protein in its original animal host and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions.
All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable. Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Learn more in our guide “How active proteins work”.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.