SDS-PAGE
(Figure 1. Human CD74(73-296) Protein, hFc Tag on SDS-PAGE under reducing condition.)
CD74 recombinant protein
Human CD74 (73-296) Protein, hFc Tag
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Purity
Test: SDS-PAGE & Coomassie blue staining ; Specification: Target protein band (s) >95.0% ; Result: PassThe Purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining
Synonyms
CD74; N/A; Human CD74 (73-296) Protein, hFc Tag; DHLAG; HLADG; Ia-GAMMA; II; p33; CD74 recombinant protein
Host
HEK293 cells
Purity/Purification
Test: SDS-PAGE & Coomassie blue staining ; Specification: Target protein band (s) >95.0% ; Result: Pass
The Purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining
The Purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose is added as protectants before lyophilization.
Concentration
2.28 mg/ml (varies by lot)
Sequence
hFc (Glu99-Ala330)+CD74 (Gln73-Met296)
Species
Human
Tag
N-Human Fc tag
Reconstitution
1.Before opening the tube cap, centrifuge the sample tube at 5000g for 3-5 min at room temperature to ensure the lyophilized sample to settle down at the bottom of the tube
2. Dissolved lyophilized protein sample in sterile water based on the recommended volume 4.39 µL
3. After adding sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette sample.
2. Dissolved lyophilized protein sample in sterile water based on the recommended volume 4.39 µL
3. After adding sterile water, cover the lid and mix them by gently tapping the tube for 5-10 times. Note: Do not vortex or vigorously pipette sample.
Visual Inspection
Test: Lyophilization ; Specification: Dried White Powder ; Result: Pass
Test: Vial Label ; Specification: Label aligned and complete ; Result: Pass
Test: Bottle Sealing (Manual Check) ; Specification: Sealed tight ; Result: Pass
Test: Vial Label ; Specification: Label aligned and complete ; Result: Pass
Test: Bottle Sealing (Manual Check) ; Specification: Sealed tight ; Result: Pass
Quantity
Test: Bradford method ; Specification: >Specified quantity ; Result: Pass
Other properties
Test: pH ; Specification: 7.4 ± 0.5 ; Result: Pass
Preparation and Storage
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).
SDS-PAGE
(Figure 1. Human CD74(73-296) Protein, hFc Tag on SDS-PAGE under reducing condition.)
SDS-PAGE
(Figure 1. Human CD74(73-296) Protein, hFc Tag on SDS-PAGE under reducing condition.)
ELISA
(Figure 1. Human CD74(73-296) Protein, hFc Tag on SDS-PAGE under reducing condition.)
ELISA
(Figure 1. Human CD74(73-296) Protein, hFc Tag on SDS-PAGE under reducing condition.)
Related Product Information for CD74 recombinant protein
Description:
Recombinant Human CD74(73-296) Protein with N-terminal human Fc tag
Background:
The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
Recombinant Human CD74(73-296) Protein with N-terminal human Fc tag
Background:
The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
The protein has a predicted molecular mass of 51.5 kDa after removal of the signal peptide.The apparent molecular mass of hFc-CD74(73-296) is approximately 55-70 kDa due to glycosylation
NCBI Official Full Name
HLA class II histocompatibility antigen gamma chain
NCBI Official Synonym Full Names
CD74 molecule, major histocompatibility complex, class II invariant chain
NCBI Official Symbol
CD74
NCBI Official Synonym Symbols
II; DHLAG; HLADG; Ia-GAMMA
NCBI Protein Information
HLA class II histocompatibility antigen gamma chain; p33; HLA-DR-gamma; MHC HLA-DR gamma chain; Ia-associated invariant chain; gamma chain of class II antigens; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histo
UniProt Protein Name
HLA class II histocompatibility antigen gamma chain
UniProt Gene Name
CD74
UniProt Synonym Gene Names
DHLAG; Ii
UniProt Entry Name
HG2A_HUMAN
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Product Notes
The CD74 cd74 (Catalog #AAA47497) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: hFc (Glu99-Ala 330)+CD74 (Gln73-Met 296). It is sometimes possible for the material contained within the vial of "CD74, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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