SDS-PAGE
RAGE Active Protein | AGER active protein
RAGE Protein, Human, Recombinant (His Tag)
Gene Names
AGER; RAGE; SCARJ1
Purity
>98% as determined by SDS-PAGE
Synonyms
RAGE; N/A; RAGE Protein, Human, Recombinant (His Tag); Human AGER/RAGE Protein (His Tag); RAGE Protein, Human; advanced glycosylation end product-specific receptor; AGER active protein
Host
HEK293 Cells
Purity/Purification
>98% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80
Source
Human
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Protein Construction
A DNA sequence encoding the mature form of human AGER (NP_001127) extracellular domain (Met1-Ala 344) was expressed with a polyhistidine tag at the C-terminus.
Reconstitution
It is recommended that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV.
Activity
1. Measured by its binding ability in a functional ELISA.
2. Immobilized recombinant human AGER-His at 10ug/mL (100uL/well) can bind biotinylated mouse His-S100A1 with a linear range of 15.6-250ng/mL.
3. Measured by its ability to bind biotinylated human S100A1 in functional ELISA.
2. Immobilized recombinant human AGER-His at 10ug/mL (100uL/well) can bind biotinylated mouse His-S100A1 with a linear range of 15.6-250ng/mL.
3. Measured by its ability to bind biotinylated human S100A1 in functional ELISA.
Sequence
Met1-Ala344
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70°C.
Store it under sterile conditions at -20°C to -80°C upon receipt. It is recommended that the protein be aliquoted into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Store it under sterile conditions at -20°C to -80°C upon receipt. It is recommended that the protein be aliquoted into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for AGER active protein
Receptor for Advanced Glycosylation End Products (RAGE, or AGER) is a member of the immunoglobulin super-family transmembrane proteins, as a signal transduction receptor which binds advanced glycation endproducts, certain members of the S100/calgranulin family of proteins, high mobility group box 1 (HMGB1), advanced oxidation protein products, and amyloid (beta-sheet fibrils). Initial studies investigating the role of RAGE in renal dysfunction focused on diabetes, neurodegenerative disorders, and inflammatory responses. However, RAGE also has roles in the pathogenesis of renal disorders that are not associated with diabetes, such as obesity-related glomerulopathy, doxorubicin-induced nephropathy, hypertensive nephropathy, lupus nephritis, renal amyloidosis, and ischemic renal injuries. RAGE represents an important factor in innate immunity against pathogens, but it also interacts with endogenous ligands, resulting in chronic inflammation. RAGE signaling has been implicated in multiple human illnesses, including atherosclerosis, arthritis, Alzheimer's disease, atherosclerosis and aging associated diseases.
References
Zhou Z, et al. (2011) RAGE and its ligands in bone metabolism. Front Biosci (Schol Ed). 3: 768-76.Mosquera JA. (2010) Role of the receptor for advanced glycation end products (RAGE) in inflammation]. Invest Clin. 51(2): 257-68.D'Agati V, et al. (2010) RAGE and the pathogenesis of chronic kidney disease. Nat Rev Nephrol. 6(6): 352-60.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
The recombinant human AGER consists of 332 amino acids and predicts a molecular weight of 35.5 kDa, It migrates as an approximately 47-53 kDa band in SDS-PAGE under reducing conditions.
NCBI Official Full Name
advanced glycosylation end product-specific receptor isoform 1
NCBI Official Synonym Full Names
advanced glycosylation end-product specific receptor
NCBI Official Symbol
AGER
NCBI Official Synonym Symbols
RAGE; SCARJ1
NCBI Protein Information
advanced glycosylation end product-specific receptor
UniProt Protein Name
Advanced glycosylation end product-specific receptor
UniProt Gene Name
AGER
UniProt Synonym Gene Names
RAGE
UniProt Entry Name
RAGE_HUMAN
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Product Notes
The AGER ager (Catalog #AAA257986) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RAGE, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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