Standard Curve (Sample)
Mouse HIF1 alpha ELISA Kit | HIF1A elisa kit
Mouse HIF1 alpha ELISA Kit
Gene Names
Hif1a; MOP1; bHLHe78; AA959795; HIF1alpha; HIF1-alpha; HIF-1-alpha
Reactivity
Mouse
Synonyms
HIF1 alpha; N/A; Mouse HIF1 alpha ELISA Kit; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8; HIF1A elisa kit
Reactivity
Mouse
Specificity
The Mouse HIF1 alpha ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse HIF1 alpha proteins within the range of 125 pg/ml - 8000 pg/ml.
Sequence Length
837
Samples
Cell culture supernates, serum, plasma, tissue homogenates
Sensitivity
< 60 pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for HIF1A elisa kit
Principle of the Assay: The AAA Biotech' Mouse HIF1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse HIF1 alpha in Cell Culture Supernates, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Mouse HIF1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and HIF1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse HIF1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HIF1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha) is the regulated member of the transcription factor heterodimer HIF-1 alpha/beta. HIF-1 alpha binds to hypoxia-response elements (HREs) in the promoters of many genes involved in the adaptation to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.
Background/Introduction: The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha) is the regulated member of the transcription factor heterodimer HIF-1 alpha/beta. HIF-1 alpha binds to hypoxia-response elements (HREs) in the promoters of many genes involved in the adaptation to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
91,874 Da
NCBI Official Full Name
hypoxia-inducible factor 1-alpha isoform 1
NCBI Official Synonym Full Names
hypoxia inducible factor 1, alpha subunit
NCBI Official Symbol
Hif1a
NCBI Official Synonym Symbols
MOP1; bHLHe78; AA959795; HIF1alpha; HIF1-alpha; HIF-1-alpha
NCBI Protein Information
hypoxia-inducible factor 1-alpha
UniProt Protein Name
Hypoxia-inducible factor 1-alpha
UniProt Gene Name
Hif1a
UniProt Synonym Gene Names
HIF-1-alpha; HIF1-alpha
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Product Notes
The Mouse HIF1A hif1a (Catalog #AAA263385) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA263385 ELISA Kit recognizes Mouse HIF1A. It is sometimes possible for the material contained within the vial of "HIF1 alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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