WB (Western Blot)
(Detection of human HIF1-alpha by western blot of HeLa and Hep-G2 cell lysate treated with 200 uM CoCl2 (+) or mock treated (-). Antibody: Rabbit anti-HIF1-alpha recombinant monoclonal antibody [BL-124-3F7] (AAA213530 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
HIF1-alpha recombinant antibody
Rabbit anti-HIF1-alpha Recombinant Monoclonal Antibody [BL-124-3F7]
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human HIF1-alpha by western blot of HeLa and Hep-G2 cell lysate treated with 200 uM CoCl2 (+) or mock treated (-). Antibody: Rabbit anti-HIF1-alpha recombinant monoclonal antibody [BL-124-3F7] (AAA213530 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human HIF1-alpha by western blot of HeLa and Hep-G2 cell lysate treated with 200 uM CoCl2 (+) or mock treated (-). Antibody: Rabbit anti-HIF1-alpha recombinant monoclonal antibody [BL-124-3F7] (AAA213530 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human HIF1-alpha by western blot of immunoprecipitates from HeLa lysate treated with 200 uM CoCl2 (+) or mock treated (-). Antibodies: Rabbit anti-HIF1-alpha recombinant monoclonal antibody [BL-124-3F7] (AAA213530 lot 3) used for IP at 20 ul/mg lysate. HIF1-alpha was also immunoprecipitated by a previous lot of this antibody (AAA213530 lot 2). For blotting immunoprecipitated HIF1-alpha, AAA213530 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human HIF1-alpha by western blot of immunoprecipitates from HeLa lysate treated with 200 uM CoCl2 (+) or mock treated (-). Antibodies: Rabbit anti-HIF1-alpha recombinant monoclonal antibody [BL-124-3F7] (AAA213530 lot 3) used for IP at 20 ul/mg lysate. HIF1-alpha was also immunoprecipitated by a previous lot of this antibody (AAA213530 lot 2). For blotting immunoprecipitated HIF1-alpha, AAA213530 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemisry)
(Detection of human Hif1-alpha in FFPE clear cell renal cell carcinoma by immunohistochemistry. Antibody: Rabbit anti-Hif1-alpha recombinant monoclonal antibody [BL-124-3F7] (AAA213530 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemisry)
(Detection of human Hif1-alpha in FFPE clear cell renal cell carcinoma by immunohistochemistry. Antibody: Rabbit anti-Hif1-alpha recombinant monoclonal antibody [BL-124-3F7] (AAA213530 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human Hif1-alpha in FFPE Hep-G2 cells treated with cobalt chloride by immunocytochemistry. Antibody: Rabbit anti-Hif1-alpha recombinant monoclonal antibody [BL-124-3F7] (AAA213530 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human Hif1-alpha in FFPE Hep-G2 cells treated with cobalt chloride by immunocytochemistry. Antibody: Rabbit anti-Hif1-alpha recombinant monoclonal antibody [BL-124-3F7] (AAA213530 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ChIP (Chromatin Immunoprecipitation)
(Localization of HIF1-alpha Binding Sites by ChIP-sequencing. Chromatin from CoCl2 treated Hep-G2 cells was immunoprecipitated with anti-HIF1 alpha antibody AAA213530 and analyzed by DNA sequencing. The figure illustrates the peak distribution of HIF1 alpha binding within a 250 Kb region of chromosome 11 as detected using anti-HIF1-alpha antibody AAA213530. ChIP-seq validation performed by Active Motif, Carlsbad, CA.)
ChIP (Chromatin Immunoprecipitation)
(Localization of HIF1-alpha Binding Sites by ChIP-sequencing. Chromatin from CoCl2 treated Hep-G2 cells was immunoprecipitated with anti-HIF1 alpha antibody AAA213530 and analyzed by DNA sequencing. The figure illustrates the peak distribution of HIF1 alpha binding within a 250 Kb region of chromosome 11 as detected using anti-HIF1-alpha antibody AAA213530. ChIP-seq validation performed by Active Motif, Carlsbad, CA.)
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Product Notes
The HIF1-alpha hif1a (Catalog #AAA213530) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-HIF1-alpha Recombinant Monoclonal Antibody [BL-124-3F7] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HIF1-alpha can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the HIF1-alpha hif1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIF1-alpha, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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