Human KIT/SCFR ELISA Kit | KIT elisa kit

Human KIT/SCFR PicoKine ELISA Kit

Cat. Num. AAA45564

• Gene Names: KIT; PBT; SCFR; C-Kit; CD117
• Reactivity: Human
• Synonyms: KIT/SCFR; N/A; Human KIT/SCFR PicoKine ELISA Kit; Mast/stem cell growth factor receptor Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD117; CD117 antigen; EC 2.7.10.1; Mast/stem cell growth factor receptor precursor; PBT; Proto oncogene tyrosine protein kinase Kit; SCFR; v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog; KIT elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human KIT
• Sequence Length: 976
• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 0.78ng/ml-50ng/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human KIT/SCFR PicoKine ELISA Kit standard curve)

Related Product Information for KIT elisa kit

Principle of the assay: human KIT ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KIT has been precoated onto 96-well plates. Standards(sf21, Q26-T520) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KIT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KIT amount of sample captured in plate.
Background: SCFR(Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

NCBI and Uniprot Product Information

• NCBI GI #: 125472
• NCBI GeneID: 3815
• UniProt Accession #: P10721; Q6IQ28; Q99662; Q9UM99; B5A956; D5LXN2; D5M931; F5H8F8
• Molecular Weight: 46,658 Da
• NCBI Official Full Name: Mast/stem cell growth factor receptor Kit
• NCBI Official Synonym Full Names: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• NCBI Official Symbol: KIT
• NCBI Official Synonym Symbols: PBT; SCFR; C-Kit; CD117
• NCBI Protein Information: mast/stem cell growth factor receptor Kit; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
• UniProt Protein Name: Mast/stem cell growth factor receptor Kit
• UniProt Gene Name: KIT
• UniProt Synonym Gene Names: SCFR; SCFR; PBT
• UniProt Entry Name: KIT_HUMAN