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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with JNK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining JNK2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining JNK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining JNK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-JNK2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of JNK2 on SHG-44 cell lysates using anti-JNK2 antibody at 1/1, 000 dilution.)
WB (Western Blot) (NADK monoclonal antibody. Western Blot analysis of NADK expression in HepG2.)
WB (Western Blot) (NADK monoclonal antibody, Western Blot analysis of NADK expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged NADK is ~0.3ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of NADK expression in transfected 293T cell line by NADK monoclonal antibody. Lane 1: NADK transfected lysate (49.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NADK monoclonal antibody. Western Blot analysis of NADK expression in NIH/3T3.)
WB (Western Blot) (NADK monoclonal antibody. Western Blot analysis of NADK expression in Raw 264.7.)
WB (Western Blot) (Western Blot detection against Immunogen (75.17kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of SW480 cells with Cytochrome P450 2D6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining Cytochrome P450 2D6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cytochrome P450 2D6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Cytochrome P450 2D6 on HepG2 cell using anti-Cytochrome P450 2D6 antibody at 1/1, 000 dilution.)
WB (Western Blot) (BUB1B monoclonal antibody Western Blot analysis of BUB1B expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (39.93kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with CDC20 rabbit purified polyclonal 1:1200 and BUB1B mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
WB (Western Blot) (Western blot analysis of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged BUB1B is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to BUB1B on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody. Lane 1: BUB1B transfected lysate (119.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of USF2 expression in transfected 293T cell line by USF2 monoclonal antibody,. Lane 1: USF2 transfected lysate (37kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (USF2 monoclonal antibody. Western Blot analysis of USF2 expression in different cell lines.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USF2 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to USF2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 1ug/ml])
Application Data (Detection limit for recombinant GST tagged USF2 is ~0.3ng/ml as a capture antibody.)
WB (Western Blot) (USF2 monoclonal antibody. Western Blot analysis of USF2 expression in PC-12.)
WB (Western Blot) (USF2 monoclonal antibody Western Blot analysis of USF2 expression in HeLa.)
USF2 (Upstream Stimulatory Factor 2, Class B Basic Helix-loop-helix Protein 12, bHLHb12, FOS-interacting Protein, FIP, Major Late Transcription Factor 2, Upstream Transcription Factor 2) (Biotin)
Gene Names
USF2; FIP; bHLHb12
Reactivity
Human, Rat
Applications
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
WB (Western Blot) (Western blot analysis of POU4F3 over-expressed 293 cell line, cotransfected with POU4F3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POU4F3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged POU4F3 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POU4F3 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of POU4F3 expression in transfected 293T cell line by POU4F3 monoclonal antibody. Lane 1: POU4F3 transfected lysate (37.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (POU4F3 monoclonal antibody Western Blot analysis of POU4F3 expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (35.75kD).)
WB (Western Blot) (BACE1 monoclonal antibody (M02), clone 2C1. Western Blot analysis of BACE1 expression in rat testis.)
WB (Western Blot) (Western Blot analysis of BACE1 expression in transfected 293T cell line by BACE1 monoclonal antibody (M01), clone 2C1.Lane 1: BACE1 transfected lysate(52.9 KDa).Lane 2: Non-transfected lysate.)
Application Data (Proximity Ligation Analysis of protein-protein interactions between MMP2 and BACE1. HeLa cells were stained with anti-MMP2 rabbit purified polyclonal 1:1200 and anti-BACE1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged BACE1 is approximately 0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
ICC (Immunocytochemistry) (ICC staining SCAI in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SCAI in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SCAI in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-SCAI antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-SCAI antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SCAI antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-SCAI antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SCAI antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-SCAI antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of SCAI on Recombinant protein using anti-SCAI antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with Cytokeratin 16 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Cytokeratin 16 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cytokeratin 16 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cytokeratin 16 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 16 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Cytokeratin 16 on human skin lysates using anti-Cytokeratin 16 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
WB (Western Blot) (Western blot analysis of ASNA1 over-expressed 293 cell line, cotransfected with ASNA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ASNA1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged ASNA1 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ASNA1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ASNA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.5ug/ml])
WB (Western Blot) (Western Blot analysis of ASNA1 expression in transfected 293T cell line by ASNA1 monoclonal antibody. Lane 1: ASNA1 transfected lysate (38.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ASNA1 monoclonal antibody. Western Blot analysis of ASNA1 expression in PC-12.)
WB (Western Blot) (Western blot analysis of SGK over-expressed 293 cell line, cotransfected with SGK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SGK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged SGK is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SGK on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human stomach tissue. [antibody concentration 5ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of SGK expression in transfected 293T cell line by SGK monoclonal antibody. Lane 1: SGK transfected lysate (48.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (73.52kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with CD44 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CD44 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-CD44 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD44 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD44 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD44 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CD44 on different lysates using anti-CD44 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-DNA Polymerase beta antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-DNA Polymerase beta antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-DNA Polymerase beta antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-DNA Polymerase beta antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of DNA Polymerase beta on Zebrafish tissue lysates using anti-DNA Polymerase beta antibody at 1/200 dilution.)
WB (Western Blot) (Western blot analysis of DNA Polymerase beta on different cell lysate using anti-DNA Polymerase beta antibody at 1/1, 000 dilution. Positive control�� Lane1: PC-12 Lane2: NIH-3T3 Lane3: A431)
WB (Western Blot) (LEPRE1 monoclonal antibody, Western Blot analysis of LEPRE1 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged LEPRE1 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LEPRE1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LEPRE1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 6ug/ml].)
WB (Western Blot) (Western Blot analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 monoclonal antibody. Lane 1: LEPRE1 transfected lysate (46kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
WB (Western Blot) (Western Blot detection against Immunogen (41.62kD).)
Application Data (Detection limit for recombinant GST tagged C1D is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to C1D on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to C1D on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml])
WB (Western Blot) (Western Blot analysis of C1D expression in transfected 293T cell line by C1D monoclonal antibody Lane 1: C1D transfected lysate (16kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (C1D monoclonal antibody Western Blot analysis of C1D expression in HeLa)
IP (Immunoprecipitation) (Immunoprecipitation of USP33 transfected lysate using anti-USP33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with USP33 rabbit polyclonal antibody.)
Application Data (Detection limit for recombinant GST tagged USP33 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USP33 on HeLa cell. [antibody concentration 20ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to USP33 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of USP33 expression in transfected 293T cell line by USP33 monoclonal antibody Lane 1: USP33 transfected lysate (103.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (Western Blot analysis of NDUFV2 expression in transfected 293T cell line by NDUFV2 monoclonal antibody. Lane 1: NDUFV2 transfected lysate (Predicted MW: 27.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in K-562.)
WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in NIH/3T3.)
WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in Raw 264.7.)
WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (Western Blot analysis of GART expression in transfected 293T cell line by GART monoclonal antibody. Lane 1: GART transfected lysate (46kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GART monoclonal antibody, Western Blot analysis of GART expression in HeLa NE.)
WB (Western Blot) (Western Blot analysis of TUBA1 expression in transfected 293T cell line by TUBA1 monoclonal antibody. Lane 1: TUBA1 transfected lysate (49.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TUBA1 monoclonal antibody. Western Blot analysis of TUBA1 expression in HeLa.)
WB (Western Blot) (Western blot analysis of TUBA1 over-expressed 293 cell line, cotransfected with TUBA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TUBA1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TUBA1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TUBA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])
WB (Western Blot) (TUBA1 monoclonal antibody. Western Blot analysis of TUBA1 expression in different Cell lines and Human Tissue.)
WB (Western Blot) (Western Blot detection against Immunogen (75.02kD).)
Application Data (Detection limit for recombinant GST tagged CDH11 is 3 ng/ml as a capture antibody.)
Application Data (Detection limit for recombinant GST tagged CDH11 is 1 ng/ml as a capture antibody.)
WB (Western Blot) (CDH11 monoclonal antibody (M05), clone 3C8. Western Blot analysis of CDH11 expression in human kidney.)
WB (Western Blot) (CDH11 monoclonal antibody (M05), clone 3C8. Western Blot analysis of CDH11 expression in MCF-7.)
Application Data (PC3-MM2 cells were stained with CDH11-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDH11 on PC3-MM2 cell. [antibody concentration 10 ug/ml])
FCM (Flow Cytometry) (Overlay histogram showing Raji cells stained with AAA28060 (red line) at 1:500. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM (Flow Cytometry) (Overlay histogram showing Jurkat cells stained with AAA28060 (red line) at 1:500. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence) (Immunofluorescence staining of U937 cells with AAA28060 at 1:250, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence) (Immunofluorescence staining of Raji cells with AAA28060 at 1:250, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IF (Immunofluorescence) (Immunofluorescence staining of Jurkat cells with AAA28060 at 1:250, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)
IHC (Immunohistchemistry) (IHC image of AAA28060 diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry) (IHC image of AAA28060 diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot) (SMAD3 monoclonal antibody. Western Blot analysis of SMAD3 expression in human colon.)
WB (Western Blot) (SMAD3 monoclonal antibody. Western Blot analysis of SMAD3 expression in HeLa.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK8 and SMAD3. HeLa cells were stained with MAPK8 rabbit purified polyclonal 1:1200 and 133539 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged SMAD3 is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of 133539 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of SMAD3 expression in transfected 293T cell line by 133539. Lane 1: SMAD3 transfected lysate (48kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of SMAD3 expression in PC-12 using 133539.)
Application Data (Detection limit for recombinant GST tagged NDUFA9 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NDUFA9 on NIH/3T3 cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NDUFA9 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.8ug/ml])
WB (Western Blot) (NDUFA9 monoclonal antibody Western Blot analysis of NDUFA9 expression in NIH/3T3.)
WB (Western Blot) (NDUFA9 monoclonal antibody. Western Blot analysis of NDUFA9 expression in Raw 264.7.)
WB (Western Blot) (NDUFA9 monoclonal antibody Western Blot analysis of NDUFA9 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (33.99kD).)
WB (Western Blot) (CAPNS1 monoclonal antibody, Western Blot analysis of CAPNS1 expression in A-431.)
WB (Western Blot) (Western blot analysis of CAPNS1 over-expressed 293 cell line, cotransfected with CAPNS1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAPNS1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CAPNS1 is ~0.1ng/ml when antibody 124328 was used as capture antibody.)
IF (Immunofluorescence) (Immunofluorescence staining of CAPNS1 on HeLa cells using antibody 124328 at 20ug/ml.)
IHC (Immunohistochemistry) (Immunoperoxidase staining of CAPNS1 on formalin-fixed paraffin-embedded human kidney using antibody 124328 at 3ug/ml.)
WB (Western Blot) (Western Blot analysis of CAPNS1 expression in transfected 293T cell line using antibody 124328: Lane 1: CAPNS1 transfected lysate (28.3kD) Lane 2: Non-transfected lysate)
WB (Western Blot) (Western Blot analysis of CAPNS1 expression in PC-12 cells using antibody 124328.)
WB (Western Blot) (ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in Jurkat.)
Application Data (Detection limit for recombinant GST tagged ABHD5 is ~1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ABHD5 expression in transfected 293T cell line by ABHD5 monoclonal antibody. Lane 1: ABHD5 transfected lysate (39kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in human liver.)
WB (Western Blot) (Western Blot detection against Immunogen (64.5kD).)
SDS-PAGE (Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-Claudin 1 (ABT-CLD1)antibody. The HRP-conjugated Goat anti-M)
SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Galectin-3 and anti-GAPDH antibody. The HRP-conjugated)
SDS-PAGE (Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-Galectin-3 antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody.)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13: Alexa Fluor 647)
Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 (red in A) and Mouse anti CD21 antibody, clone LB21 (green in B). The merged image is in C with nuclei counterstained blue using DAPI. High power.)
Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD13: APC)
Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 (red in A) and Mouse anti CD21 antibody, clone LB21 (green in B). The merged image is in C with nuclei counterstained blue using DAPI. Medium power.)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13)
Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 (red in A) and Mouse anti CD21 antibody, clone LB21 (green in B). The merged image is in C with nuclei counterstained blue using DAPI. Low power.)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 followed by Histar Detection System. Medium power)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13:RPE)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13:FITC)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 followed by Histar Detection System. Medium power)
WB (Western Blot) (HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in PC-12.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP3K7 and HSPA1L HeLa cells were stained with MAP3K7 rabbit purified polyclonal 1:1200 and HSPA1L mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged HSPA1L is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1L on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])
WB (Western Blot) (HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (34.65kD).)
WB (Western Blot) (Western blot analysis of ENO3 over-expressed 293 cell line, cotransfected with ENO3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ENO3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged ENO3 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ENO3 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ENO3 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ENO3 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5ug/ml].)
WB (Western Blot) (Western Blot analysis of ENO3 expression in transfected 293T cell line by ENO3 monoclonal antibody. Lane 1: ENO3 transfected lysate (46.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (31.24kD).)
WB (Western Blot) (Western Blot analysis of ACY1 expression in transfected 293T cell line by ACY1 monoclonal antibody. Lane 1: ACY1 transfected lysate (45.9kD). Lane 2: Non-transfected lysate.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACY1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACY1 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 3ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation of ACY1 transfected lysate using ACY1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ACY1 rabbit polyclonal antibody.)
WB (Western Blot) (ACY1 monoclonal antibody Western Blot analysis of ACY1 expression in K-562.)
WB (Western Blot) (Western Blot detection against Immunogen (70.62kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROD1 on HeLa cell. [antibody concentration 20ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ROD1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
WB (Western Blot) (ROD1 monoclonal antibody. Western Blot analysis of ROD1 expression in NIH/3T3)
WB (Western Blot) (ROD1 monoclonal antibody Western Blot analysis of ROD1 expression in Hela NE.)
WB (Western Blot) (ROD1 monoclonal antibody Western Blot analysis of ROD1 expression in PC-12)
WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)
Application Data (Detection limit for recombinant GST tagged NME1 is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (NME1 monoclonal antibody (M02), clone 1D7. Western Blot analysis of NME1 expression in different cell lines.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NME1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NME1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
ICC (Immunocytochemistry) (ICC staining CD13 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD13 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD13 in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CD13 antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD13 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-CD13 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CD13 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD13 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD13 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CD13 on different lysates using anti-CD13 antibody at 1/1, 000 dilution. Positive control: Lane 1: THP-1 Lane 2: human heart Lane 3: U937)
Application Data (Detection limit for recombinant GST tagged SNRPA is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SNRPA on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SNRPA on formalin-fixed paraffin-embedded human heart tissue. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of SNRPA expression in transfected 293T cell line by SNRPA monoclonal antibody.Lane 1: SNRPA transfected lysate (31.3kD).Lane 2: Non-transfected lysate.)
WB (Western Blot) (SNRPA monoclonal antibody, Western Blot analysis of SNRPA expression in Hela.)
WB (Western Blot) (Western Blot detection against Immunogen (57.13kD).)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between AKT1 and HSPB1. Mahlavu cells were stained with AKT1 rabbit purified polyclonal 1:1200 and HSPB1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged HSPB1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HSPB1 transfected lysate using HSPB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HSPB1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HSPB1 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of HSPB1 expression in transfected 293T cell line by HSPB1 monoclonal antibody. Lane 1: HSPB1 transfected lysate (22.8kD). Lane 2: Non-transfected lysate.)
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Purity
Purified by Protein A Affinity Chromatography.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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