Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (Western Blot detection against Immunogen (78.03kD).)
WB (Western Blot) (POLK monoclonal antibody, Western Blot analysis of POLK expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged POLK is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POLK on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody. Lane 1: POLK transfected lysate (54.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (HMGB2 monoclonal antibody (M05), clone 3E5 Western Blot analysis of HMGB2 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])
ICC (Immunocytochemistry) (ICC staining Lactate Dehydrogenase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Lactate Dehydrogenase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Lactate Dehydrogenase antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Lactate Dehydrogenase on hybrid fish (crucian-carp) brain tissue lysate using anti-Lactate Dehydrogenase antibody at 1/500 dilution.)
WB (Western Blot) (Western blot analysis of Lactate Dehydrogenase on different lysates using anti-Lactate Dehydrogenase antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: A549 Lane 3: MCF-7)
SDS-PAGE (Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Cytokeratin 5/6 (ABT-CK5/052) antibody. The HRP-conjugated Goat anti-M)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in Raw 264.7.)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged ZNF622 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ZNF622 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of ZNF622 expression in transfected 293T cell line by ZNF622 monoclonal antibody. Lane 1: ZNF622 transfected lysate (54.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (SMURF1 monoclonal antibody, Western Blot analysis of SMURF1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.55kD).)
Application Data (Detection limit for recombinant GST tagged SMURF1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of SMURF1 transfected lysate using SMURF1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with SMURF1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMURF1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMURF1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 2ug/ml].)
WB (Western Blot) (Western Blot analysis of SMURF1 expression in transfected 293T cell line by SMURF1 monoclonal antibody. Lane 1: SMURF1 transfected lysate (83.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NEK9 monoclonal antibody. Western Blot analysis of NEK9 expression in PC-12.)
WB (Western Blot) (NEK9 monoclonal antibody Western Blot analysis of NEK9 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged NEK9 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK9 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of NEK9 expression in transfected 293T cell line by NEK9 monoclonal antibody. Lane 1: NEK9 transfected lysate (107.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NEK9 monoclonal antibody. Western Blot analysis of NEK9 expression in NIH/3T3.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with p16INK4A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining p16INK4A in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining p16INK4A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining p16INK4A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.)
WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x.)
WB (Western Blot) (Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa.)
WB (Western Blot) (ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in Jurkat.)
Application Data (Detection limit for recombinant GST tagged ABHD5 is ~1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ABHD5 expression in transfected 293T cell line by ABHD5 monoclonal antibody. Lane 1: ABHD5 transfected lysate (39kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in human liver.)
WB (Western Blot) (Western Blot detection against Immunogen (64.5kD).)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using eNOS Rabbit mAb (AAA28545) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using eNOS Rabbit mAb (AAA28545) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using eNOS Rabbit mAb (AAA28545) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using eNOS Rabbit mAb (AAA28545) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using eNOS Rabbit mAb (AAA28545) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using eNOS Rabbit mAb (AAA28545) at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)
Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)
Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)
Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)
WB (Western Blot) (Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.)
WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x.)
WB (Western Blot) (Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa.)
WB (Western Blot) (DCUN1D1 monoclonal antibody, Western Blot analysis of DCUN1D1 expression in HepG2.)
WB (Western Blot) (DCUN1D1 monoclonal antibody. Western Blot analysis of DCUN1D1 expression in PC-12.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DCUN1D1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of DCUN1D1 expression in transfected 293T cell line by DCUN1D1 monoclonal antibody. Lane 1: DCUN1D1 transfected lysate (30.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (DCUN1D1 monoclonal antibody. Western Blot analysis of DCUN1D1 expression in NIH/3T3.)
WB (Western Blot) (DCUN1D1 monoclonal antibody. Western Blot analysis of DCUN1D1 expression in Raw 264.7.)
WB (Western Blot) (Western Blot detection against Immunogen (35.9kD).)
DCUN1D1 (DCN1-like Protein 1, DCUN1 Domain-containing Protein 1, Defective in Cullin Neddylation Protein 1-like Protein 1, Squamous Cell Carcinoma-related Oncogene, DCUN1L1, RP42, SCCRO) (AP)
WB (Western Blot) (Western blot analysis of ILK over-expressed 293 cell line, cotransfected with ILK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ILK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (ILK monoclonal antibody. Western Blot analysis of ILK expression in NIH/3T3.)
Application Data (Detection limit for recombinant GST tagged ILK is ~3ng/ml as a capture antibody.)
WB (Western Blot) (ILK monoclonal antibody. Western Blot analysis of ILK expression in HepG2.)
WB (Western Blot) (ILK monoclonal antibody. Western Blot analysis of ILK expression in PC-12.)
WB (Western Blot) (ILK monoclonal antibody Western Blot analysis of ILK expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (38.06kD).)
WB (Western Blot) (DCPS monoclonal antibody. Western Blot analysis of DCPS expression in PC-12.)
Application Data (Detection limit for recombinant GST tagged DCPS is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DCPS on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DCPS on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.5ug/ml])
WB (Western Blot) (DCPS monoclonal antibody Western Blot analysis of DCPS expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (63.18kD).)
WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in Hela NE.)
WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in PC-12.)
Application Data (Detection limit for recombinant GST tagged BATF is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of BATF transfected lysate using BATF monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BATF rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of BATF expression in transfected 293T cell line by BATF monoclonal antibody Lane 1: BATF transfected lysate (14.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in NIH/3T3.)
WB (Western Blot) (Western Blot detection against Immunogen (36.23kD).)
Application Data (Detection limit for recombinant GST tagged IFI16 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to IFI16 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to IFI16 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of IFI16 expression in transfected 293T cell line by IFI16 monoclonal antibody. Lane 1: IFI16 transfected lysate (82kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (IFI16 monoclonal antibody Western Blot analysis of IFI16 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged RUNX2 is approximately 10ng/ml as a capture antibody.)
WB (Western Blot) (RUNX2 monoclonal antibody (M04), clone 4D5 Western Blot analysis of RUNX2 expression in PC-12 (Cat # L012V1).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged ROCK2 is 0.3 ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ROCK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ROCK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROCK2 on HeLa cell. [antibody concentration 30 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROCK2 on HeLa cell. [antibody concentration 30 ug/ml])
WB (Western Blot) (ROCK2 monoclonal antibody (M02), clone 1E12 Western Blot analysis of ROCK2 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat lung using FADD Rabbit mAb (AAA28538) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen using FADD Rabbit mAb (AAA28538) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human lung using FADD Rabbit mAb (AAA28538) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon using FADD Rabbit mAb (AAA28538) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using FADD Rabbit mAb (AAA28538) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180s.)
WB (Western Blot) (Western blot analysis of various lysates using FADD Rabbit mAb (AAA28538) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
ICC (Immunocytochemistry) (ICC staining FMRP in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining FMRP in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining FMRP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-FMRP antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-FMRP antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FMRP antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of FMRP on different cells lysates using anti-FMRP antibody at 1/500 dilution. Positive control�� Lane 1: Hela Lane 2: K562)
WB (Western Blot) (PKNOX2 monoclonal antibody. Western Blot analysis of PKNOX2 expression in NIH/3T3.)
WB (Western Blot) (Western blot analysis of PKNOX2 over-expressed 293 cell line, cotransfected with PKNOX2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PKNOX2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PKNOX2 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of PKNOX2 expression in transfected 293T cell line by PKNOX2 monoclonal antibody. Lane 1: PKNOX2 transfected lysate (51.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PKNOX2 monoclonal antibody, Western Blot analysis of PKNOX2 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in PC-12.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP3K7 and HSPA1L HeLa cells were stained with MAP3K7 rabbit purified polyclonal 1:1200 and HSPA1L mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged HSPA1L is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1L on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])
WB (Western Blot) (HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (34.65kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PRKDC on HeLa cell. [antibody concentration 40 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PRKDC on HeLa cell. [antibody concentration 40 ug/ml])
Application Data (Detection limit for recombinant GST tagged PRKDC is 1 ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PRKDC on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PRKDC on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])
WB (Western Blot) (PRKDC monoclonal antibody (M03), clone 2A8 Western Blot analysis of PRKDC expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Histone H2B Rabbit mAb (AAA28536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Histone H2B Rabbit mAb (AAA28536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Histone H2B Rabbit mAb (AAA28536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Histone H2B Rabbit mAb (AAA28536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Histone H2B Rabbit mAb (AAA28536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Histone H2B Rabbit mAb (AAA28536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using Histone H2B Rabbit mAb (AAA28536) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 60s.)
IHC (Immunohistchemistry) (PPIF/Cyclophilin F Antibody-Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry) (PPIF/Cyclophilin F Antibody-Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot) (PPIF/Cyclophilin F Antibody-Western blot analysis of extracts. (35ug) from mouse heart tissue lysate by using anti-PPIF monoclonal antibody. (1:500))
WB (Western Blot) (PPIF/Cyclophilin F Antibody-HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY PPIF. (Right lane) cDNA for 48 hrs and lysed)
WB (Western Blot) (PPIF/Cyclophilin F Antibody-Western blot analysis of extracts. (35ug) from 4 different cell lines by using anti-PPIF monoclonal antibody. (1:500))
WB (Western Blot) (PPIF/Cyclophilin F Antibody-Equivalent amounts of cell lysates and PPIF-Knockout 293T cells were separated by SDS-PAGE and immunoblotted with anti-PPIF monoclonal antibody(1:1000). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control.)
WB (Western Blot) (Western blot analysis of CAMK4 over-expressed 293 cell line, cotransfected with CAMK4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMK4 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAMK4 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CAMK4 on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5ug/ml].)
WB (Western Blot) (Western Blot analysis of CAMK4 expression in transfected 293T cell line by CAMK4 monoclonal antibody. Lane 1: CAMK4 transfected lysate (52.14kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (CAMK4 monoclonal antibody Western Blot analysis of CAMK4 expression in Jurkat.)
WB (Western Blot) (Western Blot detection against Immunogen (77.66kD).)
WB (Western Blot) (Western blot analysis of ABCF2 over-expressed 293 cell line, cotransfected with ABCF2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ABCF2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged ABCF2 is ~0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ABCF2 transfected lysate using anti-ABCF2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ABCF2 monoclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ABCF2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of ABCF2 expression in transfected 293T cell line by ABCF2 monoclonal antibody: Lane 1: ABCF2 transfected lysate (68.53kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ABCF2 monoclonal antibody Western Blot analysis of ABCF2 expression in Hela.)
WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)
WB (Western Blot) (PRPF19 monoclonal antibody. Western Blot analysis of PRPF19 expression in NIH/3T3.)
Application Data (Detection limit for recombinant GST tagged PRPF19 is 0.3ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of PRPF19 expression in transfected 293T cell line by PRPF19 monoclonal antibody. Lane 1: PRPF19 transfected lysate (55.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PRPF19 monoclonal antibody. Western Blot analysis of PRPF19 expression in Hela NE.)
WB (Western Blot) (PRPF19 monoclonal antibody. Western Blot analysis of PRPF19 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)
Application Data (Detection limit for recombinant GST tagged PIK3R4 is approximately 0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PIK3R4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.6 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PIK3R4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.6 ug/ml])
WB (Western Blot) (PIK3R4 monoclonal antibody (M02), clone 1B5 Western Blot analysis of PIK3R4 expression in HeLa.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PIK3R4 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PIK3R4 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged UPB1 is 1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UPB1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of UPB1 expression in transfected 293T cell line by UPB1 monoclonal antibody. Lane 1: UPB1 transfected lysate (43.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (UPB1 monoclonal antibody. Western Blot analysis of UPB1 expression in Jurkat.)
WB (Western Blot) (UPB1 monoclonal antibody. Western Blot analysis of UPB1 expression in human liver.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (FOXA1 monoclonal antibody (M03), clone 4F6 Western Blot analysis of FOXA1 expression in A-431 (Cat # L015V1).)
WB (Western Blot) (FOXA1 monoclonal antibody (M03), clone 4F6 Western Blot analysis of FOXA1 expression in HepG2 (Cat # L019V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on A-431 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on A-431 cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of A549 cells with FGFR3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining FGFR3 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining FGFR3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining FGFR3 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of FGFR3 on different cells lysates using anti-FGFR3 antibody at 1/500 dilution. Positive control�� Line 1: 293 Line 2: HepG2)
WB (Western Blot) (Western blot analysis of SP110 over-expressed 293 cell line, cotransfected with SP110 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SP110 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged SP110 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of SP110 transfected lysate using SP110 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SP110 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP110 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of SP110 expression in transfected 293T cell line by SP110 monoclonal antibody. Lane 1: SP110 transfected lysate (61.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SP110 monoclonal antibody. Western Blot analysis of SP110 expression in Jurkat.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Calreticulin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining Calreticulin in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Calreticulin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Calreticulin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Calreticulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Calreticulin on different lysates using anti-Calreticulin antibody at 1/1, 000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: HL-60)
WB (Western Blot) (NEK11 monoclonal antibody. Western Blot analysis of NEK11 expression in A-431.)
WB (Western Blot) (NEK11 monoclonal antibody, Western Blot analysis of NEK11 expression in HL-60.)
Application Data (Detection limit for recombinant GST tagged NEK11 is 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK11 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NEK11 on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of NEK11 expression in transfected 293T cell line by NEK11 monoclonal antibody. Lane 1: NEK11 transfected lysate (55.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (79.13kD).)
NEK11 (Serine/Threonine-protein Kinase Nek11, Never in Mitosis A-related Kinase 11, NimA-related Protein Kinase 11, FLJ23495) (HRP)
Reactivity
Human
Applications
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Purity
Purified by Protein A Affinity Chromatography.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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