Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (CSK monoclonal antibody. Western Blot analysis of CSK expression in Hela NE.)
WB (Western Blot) (Western Blot analysis of CSK expression in transfected 293T cell line by CSK monoclonal antibody. Lane 1: CSK transfected lysate (50.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of CSK over-expressed 293 cell line, cotransfected with CSK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CSK is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of CSK transfected lysate using CSK monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CSK rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSK on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (Western Blot detection against Immunogen (60.94kD).)
IP (Immunoprecipitation) (Immunoprecipitation of ANXA5 transfected lysate using ANXA5 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ANXA5 rabbit polyclonal antibody.)
Application Data (Detection limit for recombinant GST tagged ANXA5 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ANXA5 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ANXA5 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ANXA5 expression in transfected 293T cell line by ANXA5 monoclonal antibody. Lane 1: ANXA5 transfected lysate (35.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ANXA5 monoclonal antibody, Western Blot analysis of ANXA5 expression in Hela.)
Application Data (Proximity Ligation Analysis of protein-protein interactions between PDGFRB and PLCG1. Mahlavu cells were stained with anti-PDGFRB rabbit purified polyclonal 1:600 and anti-PLCG1 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between HCK and PLCG1. Huh7 cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-PLCG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between PTK2 and PLCG1 HeLa cells were stained with PTK2 rabbit purified polyclonal 1:1200 and PLCG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged PLCG1 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PLCG1 on HeLa cell. [antibody concentration 40ug/ml].)
WB (Western Blot) (PLCG1 monoclonal antibody. Western Blot analysis of PLCG1 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TESK2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.7 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.7 ug/ml])
WB (Western Blot) (Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M08), clone 5G1.Lane 1: TESK2 transfected lysate(60.3 KDa).Lane 2: Non-transfected lysate.)
ICC (Immunocytochemistry) (ICC staining PYK2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PYK2 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PYK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PYK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PYK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PYK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PYK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PYK2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PYK2 on mouse brain lysates using anti-PYK2 antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining DDB1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining DDB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-DDB1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-DDB1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution. Positive control: Lane 1: HepG2 Lane 2: NIH-3T3 Lane 3: MCF-7 Lane 4: Rat kidney tissue)
Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (YY1 monoclonal antibody (M03), clone 4D2 Western Blot analysis of YY1 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with PCNA antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-PCNA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-PCNA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PCNA on different cell lysates using anti-PCNA antibody at 1/1000 dilution. Positive control: Line 1: L929 Line 2 :MCF-7 Line 3:PC12 Line 4:Raji Line 5:F9 Line 6:A549)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Cortactin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Cortactin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cortactin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cortactin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cortactin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cortactin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cortactin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Cortactin on different lysates using anti-Cortactin antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: A431)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with SDHB antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-SDHB antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SDHB antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SDHB antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHB antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-SDHB antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of SDHB on different lysates using anti-SDHB antibody at 1/500 dilution. Positive control: Lane 1: Human liver Lane 2: Rat spleen Lane 3: Rat liver Lane 4: Mouse spleen Lane 5: HepG2)
ICC (Immunocytochemistry) (ICC staining NGAL in SW480 cells (red). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NGAL in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NGAL in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded huamn liver tissue using anti-NGAL antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-NGAL antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NGAL Tubulin antibody. Counter stained with hematoxylin.)
ICC (Immunocytochemistry) (ICC staining HDAC9 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining HDAC9 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining HDAC9 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HDAC9 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HDAC9 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HDAC9 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of HDAC9 on different lysates using anti-HDAC9 antibody at 1/1, 000 dilution. Positive control: Lane 1: K562 Lane 2: HepG2 Lane 3: Raji)
WB (Western Blot) (Western blot analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged GATA2 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GATA2 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody. Lane 1: GATA2 transfected lysate (50.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GATA2 monoclonal antibody, Western Blot analysis of GATA2 expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (36.96kD).)
WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in PC-12.)
WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged PPIE is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PPIE on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of PPIE expression in transfected 293T cell line by PPIE monoclonal antibody. Lane 1: PPIE transfected lysate (33.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in NIH/3T3.)
WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in Raw 264.7.)
Cyclophilin E (Cyclophilin 33, CYP33, CYP-33, MGC3736, MGC111222, Peptidyl-Prolyl Cis-Trans Isomerase E, PPIase E, PPIE, Rotamase E, Rotamase-E) (Biotin)
Gene Names
PPIE; CYP33; CYP-33
Reactivity
Human, Mouse, Rat
Applications
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Data (Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (HDAC1 monoclonal antibody (M06), clone 1D6 Western Blot analysis of HDAC1 expression in Hela S3 NE.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (YY1 monoclonal antibody (M04), clone 2C5 Western Blot analysis of YY1 expression in Hela S3 NE (Cat # L013V3).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 7 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 7 ug/ml])
Application Data (Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (NFKB1 monoclonal antibody (M03), clone 3F6 Western Blot analysis of NFKB1 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
ICC (Immunocytochemistry) (ICC staining CD130 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD130 (green) in HUVEC cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CD130 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD130 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD130 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD130 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CD130 on Raji cell (1) and mouse lung tissue (2) lysates using anti-CD130 antibody at 1/500 dilution.)
WB (Western Blot) (Western blot analysis of CD130 on CD130 recombinant protein using anti-CD130 antibody at 1/5, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of 293 cells with RALBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-RALBP1 antibody. Counter stained with hematoxylin.)
SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-MUM1 (ABT080)antibody. The HRP-conjugated Goat anti-M)
ICC (Immunocytochemistry) (ICC staining VAV2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining VAV2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining VAV2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VAV2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-VAV2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of VAV2 on SW480 cells lysates using anti-VAV2 antibody at 1/1, 000 dilution.)
Application Data (Published clone specific image Alloimmunity-associated cytotoxicity is mediated through the NKG2D receptor. (A) Liver expression of nkg2d on day ten after liver transplantation. (B) Representative NKG2D expression levels in blood NK cells (left) and monocytes (right) of allogeneic (black) and syngeneic (grey) recipients. Isotype was used as control (dashed lines). (C) Sorted blood NK cell cytotoxicity inhibition with anti-NKG2D antibody or with anti-NKp30 antibody. (D) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in the liver on day ten after transplantation. (E) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in rat HCC cell lines. (F) Representative level of recombinant NKG2D-Fc binding to rat HCC cells lines. *p)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Low power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power)
Application Data (Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. Medium power)
Application Data (Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. Low power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Medium power)
Application Data (Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. High power)
WB (Western Blot) (LETM1 monoclonal antibody Western Blot analysis of LETM1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.62kD).)
Application Data (Detection limit for recombinant GST tagged LETM1 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of LETM1 transfected lysate using LETM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LETM1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LETM1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LETM1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of LETM1 expression in transfected 293T cell line by LETM1 monoclonal antibody.Lane 1: LETM1 transfected lysate (83.4kD).Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of FOXA2 over-expressed 293 cell line, cotransfected with FOXA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FOXA2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged FOXA2 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of FOXA2 transfected lysate using FOXA2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FOXA2 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HepG2 cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of FOXA2 expression in transfected 293T cell line by FOXA2 monoclonal antibody Lane 1: FOXA2 transfected lysate (48.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (FOXA2 monoclonal antibody Western Blot analysis of FOXA2 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (36.19kD).)
WB (Western Blot) (ALS2CR8 monoclonal antibody, Western Blot analysis of ALS2CR8 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (36.89kD).)
WB (Western Blot) (Western blot analysis of ALS2CR8 over-expressed 293 cell line, cotransfected with ALS2CR8 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ALS2CR8 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged ALS2CR8 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ALS2CR8 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ALS2CR8 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot analysis of ALS2CR8 expression in transfected 293T cell line by ALS2CR8 monoclonal antibody. Lane 1: ALS2CR8 transfected lysate (79.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (G3BP monoclonal antibody Western Blot analysis of G3BP expression in A-431)
WB (Western Blot) (Western blot analysis of G3BP over-expressed 293 cell line, cotransfected with G3BP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with G3BP monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to G3BP on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to G3BP on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 1ug/ml])
WB (Western Blot) (Western Blot analysis of G3BP expression in transfected 293T cell line by G3BP monoclonal antibody Lane 1: G3BP transfected lysate (52.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
WB (Western Blot) (CSNK2A1 monoclonal antibody, Western Blot analysis of CSNK2A1 expression in Hela NE.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between TP53 and CSNK2A1 HeLa cells were stained with TP53 rabbit purified polyclonal 1:1200 and CSNK2A1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged CSNK2A1 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSNK2A1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of CSNK2A1 expression in transfected 293T cell line by CSNK2A1 monoclonal antibody. Lane 1: CSNK2A1 transfected lysate (45.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)
WB (Western Blot) (TRIM33 monoclonal antibody, Western Blot analysis of TRIM33 expression in Hela NE)
WB (Western Blot) (TRIM33 monoclonal antibody. Western Blot analysis of TRIM33 expression in 293)
Application Data (Detection limit for recombinant GST tagged TRIM33 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TRIM33 on HeLa cell. [antibody concentration 30ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TRIM33 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])
WB (Western Blot) (TRIM33 monoclonal antibody. Western Blot analysis of TRIM33 expression in PC-12)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Detection limit for recombinant GST tagged STIP1 is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (STIP1 monoclonal antibody (M11), clone 1E3 Western Blot analysis of STIP1 expression in HeLa.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml])
WB (Western Blot) (SMAD3 monoclonal antibody (M01), clone 2C12. Western Blot analysis of SMAD3 expression in Hs 181.Tes.)
WB (Western Blot) (SMAD3 monoclonal antibody (M01), clone 2C12. Western Blot analysis of SMAD3 expression in HeLa.)
WB (Western Blot) (Western Blot analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M01), clone 2C12.Lane 1: SMAD3 transfected lysate (48 KDa).Lane 2: Non-transfected lysate.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMAD3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 6 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMAD3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 6 ug/ml])
WB (Western Blot) (SMAD3 monoclonal antibody (M01), clone 2C12. Western Blot analysis of SMAD3 expression in human colon.)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Mouse colon tissue using Phospho-STAT3-Y705 Rabbit mAb (AAA28610, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse colon using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at 1:1000 dilution. NIH/3T3 cells were treated by TNF-? (20 ng/mL) at 37? for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180s.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with SQSTM1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining SQSTM1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-SQSTM1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of SQSTM1 on different cell lysates using anti-SQSTM1 antibody at 1/500 dilution. Positive control: </br>Lane1: Hela </br>Lane2: HepG2)
ICC (Immunocytochemistry) (ICC staining CD14 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD14 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD14 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD14 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD14 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD14 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD14 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD14 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD14 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CD14 on different lysates using anti-CD14 antibody at 1/1, 000 dilution. Positive control: Lane 1: Human liver Lane 2: SW480)
FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells with SAMHD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining SAMHD1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SAMHD1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SAMHD1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-SAMHD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SAMHD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SAMHD1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of SAMHD1 on THP-1 cell lysate using anti-SAMHD1 antibody at 1/1, 000 dilution.)
SDS-PAGE (HCT116whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK20(ABT006) antibody. The HRP-conjugated Goat anti-M)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TESK2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])
WB (Western Blot) (Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M04), clone 5H4.Lane 1: TESK2 transfected lysate(60.3 KDa).Lane 2: Non-transfected lysate.)
FCM (Flow Cytometry) (Flow cytometric analysis of HeLa cells with beta-tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining��-tubulin in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC stainingbeta-tubulin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining beta-tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-beta-tubulin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of beta-tubulin on different cell lysates using anti-beta-tubulin antibody at 1/5000 dilution. Positive control: Lane 1: NCCIT Lane 2: NIH/3T3 Lane 3: PC12 Lane 4: Mouse heart Lane 5: F9 Lane 6: zebrafish brain Lane 7: Hela)
ICC (Immunocytochemistry) (ICC staining PSMA1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PSMA1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PSMA1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PSMA1 on different lysates using anti-PSMA1 antibody at 1/500 dilution. Positive control: Lane 1: PC-12 Lane 2: Mouse spleen tissue Lane 3: Rat spleen tissue)
WB (Western Blot) (Western blot analysis of PSMA1 on different cell lysates using anti-PSMA1 antibody at 1/500 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: HepG2 Lane 4: 293)
SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-HMB45 (ABT255) antibody. The HRP-conjugated Goat anti-M)
Application Data (Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
Application Data (Published customer image: CD11b immunoreactive (red) microglia in the motor cortex following cervical corticospinal tract injury. Representative images of motor cortex - a, b: one week (deconvolved images); c, d: two weeks following corticospinal tract injury. The corticospinal neurons were retrogradely labelled with fluorogold applied to the spinal cord at the time of injury. There is little sign of microglial activation. The inset in (c) shows a CD6-positive T-cell in the motor cortex, but no accumulation of such cells was detected.From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN. Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration. BMC Neurosci. 2010 Feb 5;11:13.)
Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD11b)
Application Data (Published customer image: Microglia in the red nucleus (a-d and inset) and motor cortex (e, f) 4 weeks after nerve graft implantation into the rubrospinal tract and cervical dorsal columns respectively. Neurons with regenerating axons were retrogradely labelled with fluorogold. Microglia established close contacts with regenerating rubrospinal neurons (arrows), which also expressed ATF3 (inset). No retrogradely labelled corticospinal neurons were detected (e and f) and there was no microglial activation in the motor cortex. (a-c) are non-deconvolved and (d-f plus inset) are deconvolved images. The scale bar in (a) also applies to (b).From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN. Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration. BMC Neurosci. 2010 Feb 5;11:13.)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power)
Application Data (Staining of rat peritoneal macrophages cells with Mouse anti Rat CD11b:Alexa Fluor 488)
Application Data (Published customer image: Rubrospinal injury produces little microglial activation and no accumulation of T-cells in the red nucleus. a, b: Representative images show there is little difference in CD11b immunoreactivity for microglia in the red nucleus (*) one week following axotomy. c, d: confocal images of beta thymosin immunoreactive microglia in the red nucleus 3 weeks after injury. e: a rare CD6-positive T lymphocyte in the red nucleus of an unoperated rat. f: one week after injury no T lymphocytes can be identified in the red nucleus around the axotomized rubrospinal neurons, which can be recognised by their expression of ATF3 (green). Neuronal cytoplasm has been visualised by high gain in the red signal; this does not represent CD6 signal.From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN. Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration. BMC Neurosci. 2010 Feb 5;11:13.)
Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD11b:RPE-Alexa Fluor 647)
Application Data (Immunofluoescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained in blue using DAPI. High power)
Application Data (Immunofluoescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained in blue using DAPI. Low power)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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