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ICC (Immunocytochemistry) (ICC staining PP2A alpha + beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PP2A alpha + beta in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PP2A alpha + beta antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PP2A alpha + beta on hybrid fish (crucian-carp) brain tissue lysate using anti-PP2A alpha + beta antibody at 1/500 dilution.)
WB (Western Blot) (Western blot analysis of PP2A alpha + beta on different lysates using anti-PP2A alpha + beta antibody at 1/1, 000 dilution. Positive control: Lane 1: A431 Lane 2: NIH/3T3 Lane 3: 293T)
FCM (Flow Cytometry) (Flow cytometry analysis of GOT1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
FCM (Flow Cytometry) (Flow cytometry analysis of GOT1 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
ICC (Immunocytochemistry) (ICC/IF analysis of GOT1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
ICC (Immunocytochemistry) (ICC/IF analysis of GOT1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : 293T cell lysate Lane 2 : GOT1 Transfected 293T cell lysate)
WB (Western Blot) (The recombinat proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant human GOT1 Lane 2 : Recombinant human GOT2)
WB (Western Blot) (The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : A427 cell lysate)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. Med power)
Application Data (Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Medium power)
Application Data (Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Low power)
Application Data (Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. High power)
Application Data (Staining of rat splenocytes with Mouse anti Rat CD43: FITC)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. High power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. Low power)
WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in HeLa.)
WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in Raw 264.7.)
Application Data (Detection limit for recombinant GST tagged DNAJC10 is ~10ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DNAJC10 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DNAJC10 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)
WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in NIH/3T3.)
WB (Western Blot) (Western Blot detection against Immunogen (37.77kD).)
WB (Western Blot) (Western blot analysis of CAMK4 over-expressed 293 cell line, cotransfected with CAMK4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMK4 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAMK4 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CAMK4 on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5ug/ml].)
WB (Western Blot) (Western Blot analysis of CAMK4 expression in transfected 293T cell line by CAMK4 monoclonal antibody. Lane 1: CAMK4 transfected lysate (52.14kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (CAMK4 monoclonal antibody Western Blot analysis of CAMK4 expression in Jurkat.)
WB (Western Blot) (Western Blot detection against Immunogen (77.66kD).)
WB (Western Blot) (Western blot analysis of PSMC6 over-expressed 293 cell line, cotransfected with PSMC6 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PSMC6 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PSMC6 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of PSMC6 expression in transfected 293T cell line by PSMC6 monoclonal antibody. Lane 1: PSMC6 transfected lysate (44.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PSMC6 monoclonal antibody. Western Blot analysis of PSMC6 expression in NIH/3T3.)
WB (Western Blot) (PSMC6 monoclonal antibody Western Blot analysis of PSMC6 expression in Hela NE.)
WB (Western Blot) (PSMC6 monoclonal antibody. Western Blot analysis of PSMC6 expression in PC-12.)
ICC (Immunocytochemistry) (ICC staining EAAT3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining EAAT3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining EAAT3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of EAAT3 on mouse liver tissue lysate using anti-EAAT3 antibody at 1/500 dilution.)
IP (Immunoprecipitation) (Immunoprecipitation of PSMA7 transfected lysate using PSMA7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSMA7 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PSMA7 on HeLa cell. [antibody concentration 1 ~ 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 5ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosqumous cell carcinoma. [antibody concentration 5ug/ml])
WB (Western Blot) (Western Blot analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody. Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PSMA7 monoclonal antibody Western Blot analysis of PSMA7 expression in HeLa.)
WB (Western Blot) (PSMA7 monoclonal antibody. Western Blot analysis of PSMA7 expression in human omentum, serous carcinoma.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with AOF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
ICC (Immunocytochemistry) (ICC staining AOF1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of AOF1 on HEK293 (1) and AOF1-hIgGFc transfected HEK293 (2) cell lysate using anti-AOF1 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of AOF1 on human AOF1 recombinant protein using anti-AOF1 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western Blot detection against Immunogen (60.94kD).)
IP (Immunoprecipitation) (Immunoprecipitation of ANXA5 transfected lysate using ANXA5 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ANXA5 rabbit polyclonal antibody.)
Application Data (Detection limit for recombinant GST tagged ANXA5 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ANXA5 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ANXA5 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ANXA5 expression in transfected 293T cell line by ANXA5 monoclonal antibody. Lane 1: ANXA5 transfected lysate (35.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ANXA5 monoclonal antibody, Western Blot analysis of ANXA5 expression in Hela.)
WB (Western Blot) (Western Blot analysis of TUBA1 expression in transfected 293T cell line by TUBA1 monoclonal antibody. Lane 1: TUBA1 transfected lysate (49.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TUBA1 monoclonal antibody. Western Blot analysis of TUBA1 expression in HeLa.)
WB (Western Blot) (Western blot analysis of TUBA1 over-expressed 293 cell line, cotransfected with TUBA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TUBA1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TUBA1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TUBA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])
WB (Western Blot) (TUBA1 monoclonal antibody. Western Blot analysis of TUBA1 expression in different Cell lines and Human Tissue.)
WB (Western Blot) (Western Blot detection against Immunogen (75.02kD).)
Application Data (Detection limit for recombinant GST tagged SDCBP is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of SDCBP transfected lysate using SDCBP monoclonal antibody and Protein A Magnetic Bead and immunoblotted with SDCBP rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SDCBP on HepG2 cell. [antibody concentration 35ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SDCBP on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of SDCBP expression in transfected 293T cell line by SDCBP monoclonal antibody. Lane 1: SDCBP transfected lysate (32.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SDCBP monoclonal antibody, Western Blot analysis of SDCBP expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PRKDC on HeLa cell. [antibody concentration 40 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PRKDC on HeLa cell. [antibody concentration 40 ug/ml])
Application Data (Detection limit for recombinant GST tagged PRKDC is 1 ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PRKDC on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PRKDC on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])
WB (Western Blot) (PRKDC monoclonal antibody (M03), clone 2A8 Western Blot analysis of PRKDC expression in Hela S3 NE.)
FCM (Flow Cytometry) (Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using PD-L2 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 2 μg/mL.)
IHC (Immunohistchemistry) (Immunohistochemistry of PD-L2 in human tonsil tissue with PD-L2 antibody at 2 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [8C12]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of PD-L2 in human tonsil tissue with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [8C12]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [8C12]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.)
WB (Western Blot) (Western blot analysis of PD-L2 in overexpressing HEK293 cells PD-L2 antibody at 0.5 and 1 μg/ml)
WB (Western Blot) (Western Blot analysis using CD44 antibodyWestern Blot showing using CD44 antibody used against Hela (1) and HUVE-12 (2) cell lysate.)
WB (Western Blot) (Western Blot analysis using CD44 antibodyWestern Blot showing CD44 antibody used against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).)
IHC (Immunohistochemistry) (Immunohistochemical staining using CD44 antibodyImmunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using CD44 antibody.)
IF (Immunofluorescence) (Immunofluorescent staining using Western Blot analysis using CD44 antibodyConfocal immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using CD44 antibody (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.)
IF (Immunofluorescence) (Immunofluorescent staining using Western Blot analysis using CD44 antibodyConfocal analysis of paraffin-embedded human lung cancer tissues using CD44 antibody (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.)
FCM (Flow Cytometry) (Flow Cytometric assay using Western Blot analysis using CD44 antibodyFC analysis of Hela cells using CD44 antibody (right) and negative control (left).)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
Application Data (Detection limit for recombinant GST tagged GMNN is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GMNN on HeLa cell . [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of GMNN expression in transfected 293T cell line by GMNN monoclonal antibody.Lane 1: GMNN transfected lysate (23.6kD).Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFIC on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody. Lane 1: NFIC transfected lysate (47.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NFIC monoclonal antibody Western Blot analysis of NFIC expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (72.82kD).)
WB (Western Blot) (Western blot analysis of RAB7 on different lysates using anti-RAB7 antibody at 1/1, 000 dilution. Positive control: Lane 1: A431 Lane 2: C2C12)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-RAB7 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-RAB7 antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RAB7 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-RAB7 antibody. Counter stained with hematoxylin.)
ICC (Immunocytochemistry) (ICC staining RAB7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining RAB7 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining RAB7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells with RAB7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
WB (Western Blot) (KIF2C monoclonal antibody Western Blot analysis of KIF2C expression in HeLa NE.)
WB (Western Blot) (Western Blot analysis of KIF2C expression in transfected 293T cell line by KIF2C monoclonal antibody Lane 1: KIF2C transfected lysate (81.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of KIF2C over-expressed 293 cell line, cotransfected with KIF2C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with KIF2C monoclonal antibody (M01). GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged KIF2C is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of KIF2C transfected lysate using KIF2C monoclonal antibody and Protein A Magnetic Bead and immunoblotted with KIF2C rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to KIF2C on HeLa cell. [antibody concentration 10ug/ml].)
Application Data (Immunoperoxidase of monoclonal antibody to KIF2C on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B. [antibody concentration 3ug/ml].)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-CD81 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CD81 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD81 antibody. Counter stained with hematoxylin.)
ICC (Immunocytochemistry) (ICC staining CD81 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD81 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD81 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD81 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with CD81 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of CD81 on different lysates using anti-CD81 antibody at 1/1, 000 dilution. Positive control: Lane 1: JAR Lane 2: PC-12 Lane 3: K562)
FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells with Septin 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Septin 2 in SHSY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Septin 2 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Septin 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Septin 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Septin 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Septin 2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Septin 2 on different cell lysates using anti-Septin 2 at 1/500 dilution. Positive control�� Line 1: Mouse brain Line 2: SiHa Line 3: K562)
WB (Western Blot) (Western blot analysis of lysates from 293, Hela, mouse NIH/3T3, rat PC-12 cell line and rat brain tissue lysate(from left to right), using RPS6 Antibody (N-term). AAA28643 was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded M. kidney section using RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H. kidney section using RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H. brain section using RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IF (Immunofluorescence) (Fluorescent image of Hela cells stained with RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells using RPS6 Antibody (N-term)(green) compared to an isotype control of mouse IgG1(blue). AAA28643 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
ELISA (Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA abs at decreasing concentrations.)
FCM (Flow Cytometry) (Flow cytometry analysis of PD-1 overexpressing 293 cells using at 1 μg/ml. Blue: untransfected cells, Yellow: PD-1 transfected cells.)
WB (Western Blot) (Western blot analysis of PD-1 in overexpressing 293 cells using antibody at 1, 0.5, and 0.25 μg/ml, respectively.)
IF (Immunofluorescence) (Immunofluorescence of PD-1 in transfected 293 cells with PD-1 antibody at 5 μg/mL. Lower left: Immunofluorescence in transfected 293 cells with control mouse IgG antibody at 5 μg/mL.Red: PD1 Antibody [7H6]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 5 μg/mL.)
IHC (Immunohistochemistry) (Immunohistochemistry of PD-1 in (A) human breast cancer tissue and (B) human normal breast tissue with PD-1 antibody at 5 μg/mL.)
IHC (Immunohistochemistry) (Immunohistochemistry of PD-1 in (A) human tonsil tissue, (B) human lymph node tissue, and (C) human spleen tissue with PD-1 antibody at 5 μg/mL. (D) Immunohistochemistry in human tonsil tissue with control mouse IgG staining at 5 μg/mL.)
WB (Western Blot) (Western blot analysis of PD-1 in transfected 293 cell lysate with PD-1 antibody at 1 μg/mL.)
Application Data (Detection limit for recombinant GST tagged NODAL is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3ug/ml])
WB (Western Blot) (NODAL monoclonal antibody Western Blot analysis of NODAL expression in PC-12.)
WB (Western Blot) (NODAL monoclonal antibody Western Blot analysis of NODAL expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (33.66kD).)
WB (Western Blot) (KHDRBS1 monoclonal antibody Western Blot analysis of KHDRBS1 expression in A-431)
Application Data (Detection limit for recombinant GST tagged KHDRBS1 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to KHDRBS1 on A-431 cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to KHDRBS1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of KHDRBS1 expression in transfected 293T cell line by KHDRBS1 monoclonal antibody. Lane 1: KHDRBS1 transfected lysate (41kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
WB (Western Blot) (AKT1 monoclonal antibody Western Blot analysis of AKT1 expression in NIH/3T3.)
WB (Western Blot) (Western Blot analysis of AKT1 expression in transfected 293T cell line by AKT1 monoclonal antibody. Lane 1: AKT1 transfected lysate (55.7kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged AKT1 is ~3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of AKT1 transfected lysate using AKT1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with AKT1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 20ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKT1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (78.54kD).)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 1.2 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 1.2 ug/ml])
Application Data (Detection limit for recombinant GST tagged TAF11 is approximately 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TAF11 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TAF11 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (TAF11 monoclonal antibody (M03), clone 3H5 Western Blot analysis of TAF11 expression in Hela S3 NE.)
ICC (Immunocytochemistry) (ICC staining Calnexin in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Calnexin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Calnexin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calnexin antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Calnexin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Calnexin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Calnexin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Calnexin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Calnexin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Calnexin on Hela cells lysates using anti-Calnexin antibody at 1/1, 000 dilution.)
Application Data (Figure A. FITC conjugated mouse anti bovine CD2 and RPE conjugated mouse IgG1 isotype control . Figure B. FITC conjugated mouse anti bovine CD2 and RPE conjugated mouse anti bovine CD335 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)
Application Data (Figure A. RPE conjugated mouse anti bovine CD335 and FITC conjugated mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti bovine CD335 and FITC conjugated mouse anti bovine CD2 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)
Application Data (Figure A. RPE conjugated mouse anti bovine CD8 and FITC conjugated mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti bovine CD4 and FITC conjugated mouse anti bovine CD2 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)
Application Data (Figure A. FITC conjugated mouse anti bovine CD2 and RPE conjugated mouse IgG2a isotype control . Figure B. FITC conjugated mouse anti bovine CD2 and RPE conjugated mouse anti bovine CD8 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)
Application Data (Figure A. RPE conjugated mouse anti bovine CD4 and FITC conjugated mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti bovine CD4 and FITC conjugated mouse anti bovine CD2 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells)
Application Data (Figure A. FITC conjugated mouse anti bovine CD2 and RPE conjugated mouse IgG2a isotype control . Figure B. FITC conjugated mouse anti bovine CD2 and RPE conjugated mouse anti bovine CD4 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)
Application Data (Figure A. Alexa Fluor 647 conjugated mouse anti bovine CD4 and FITC conjugated mouse IgG1 isotype control . Figure B. Alexa Fluor 647 conjugated mouse anti bovine CD4 and FITC conjugated mouse anti bovine CD2 . All experiments performed on red cell lysed bovine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
WB (Western Blot) (Detection of mouse Sodium Potassium ATPase alpha-1 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, BW5147.3, TCMK-1, and CH27 cells prepared using NETN lysis buffer. Antibody: Mouse anti-Sodium Potassium ATPase alpha-1-Recombinant monoclonal antibody [BL-464.6] (AAA23784 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot) (Detection of human Sodium Potassium ATPase alpha-1 by western blot. Samples: Whole cell lysate (10 ug) from MDA-MB-435, LNCaP, UACC-62, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Mouse anti-Sodium Potassium ATPase alpha-1 monoclonal antibody [BL-464.6] (AAA23784 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation) (Detection of human Sodium Potassium ATPase alpha-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibody: Mouse anti-Sodium Potassium ATPase alpha-1-Recombinant monoclonal antibody [BL-464.6] (AAA23784 lot 1) used for IP at 20 ul/mg lysate. For blotting immunoprecipitated Sodium Potassium ATPase alpha-1, A500-031 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry) (Detection of human Sodium Potassium ATPase alpha-1 by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Mouse anti-Sodium Potassium ATPase alpha-1 monoclonal antibody [464.6] (AAA23784 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG .)
IHC (Immunohistochemistry) (Detection of mouse Sodium Potassium ATPase alpha-1 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Mouse anti-Sodium Potassium ATPase alpha-1 monoclonal antibody [464.6] (AAA23784 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG .)
ICC (Immunocytochemistry) (Detection of human Sodium Potassium ATPase alpha-1 by immunocytochemistry. Sample: FFPE section of MDA-MB-435 cells.Antibody: Mouse anti-Sodium Potassium ATPase alpha-1 monoclonal antibody [464.6] (AAA23784 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG .)
WB (Western Blot) (Western Blot detection against Immunogen (36.41kD).)
Application Data (Detection limit for recombinant GST tagged TSC22D3 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of TSC22D3 transfected lysate using TSC22D3 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with TSC22D3 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TSC22D3 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TSC22D3 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of TSC22D3 expression in transfected 293T cell line by TSC22D3 monoclonal antibody. Lane 1: TSC22D3 transfected lysate (22.2kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged TCF7L2 is approximately 0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (TCF7L2 monoclonal antibody (M06), clone 3A4 Western Blot analysis of TCF7L2 expression in K-562.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of PD-L2 in human colon carcinoma tissue with PD-L2antibody at 20 ?g/mL. Green: PDL2 Antibody [4E10] (AAA10985)Blue: DAPI staining)
FCM (Flow Cytometry) (Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using PD-L2 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 2 μg/mL.)
IHC (Immunohistchemistry) (Immunohistochemistry of PD-L2 in human tonsil tissue with PD-L2 antibody at 2 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [4E10]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of PD-L2 in human tonsil tissue with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [4E10]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [4E10]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.)
WB (Western Blot) (PBK monoclonal antibody (M07), clone 3A7. Western Blot analysis of PBK expression in 293 (Cat # L026V1).)
WB (Western Blot) (PBK monoclonal antibody (M07), clone 3A7 Western Blot analysis of PBK expression in Hela S3 NE (Cat # L013V3).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PBK on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PBK on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PBK on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to PBK on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with Cdk4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Cdk4 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cdk4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cdk4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Cdk4 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cdk4 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Cdk4 on Hela cells lysates using anti-Cdk4 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Detection of human CD20 by western blot. Samples: Whole cell lysate (25 ug) from Ramos and MCF-7 cells prepared using NETN lysis buffer. Antibody: Mouse anti-CD20 monoclonal antibody [L26] (AAA23779 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IHC (Immunohistochemistry) (Detection of human CD20 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse anti-CD20 monoclonal antibody [L26] (AAA23779 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry) (Detection of human CD20 by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD20 antibody [L26] (AAA23779 lot 1) used at 1:250. Secondary: DyLight 594-conjugated goat anti-mouse IgG .)
IHC (Immunohistochemistry) (Detection of human CD20 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Purified mouse monoclonal anti-CD20 (Clone L26) antibody (AAA23779 Lot2) used at a dilution of 1:500. Detection: DAB. Counterstain: IHC Hematoxylin (blue).)
ICC (Immunocytochemistry) (Detection of human CD20 by immunocytochemistry. Sample: FFPE section of VLN3G2 cells. Antibody: Purified mouse monoclonal anti-CD20 (Clone L26) antibody (AAA23779 Lot2) used at a dilution of 1:500. Detection: DAB. Counterstain: IHC Hematoxylin (blue).)
FCM (Flow Cytometry) (Detection of human CD20 (shaded) in Raji cells by flow cytometry. Antibody: Mouse anti-CD20 monoclonal antibody [L26] (AAA23779) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-mouse IgG .)
WB (Western Blot) (AKR1B10 monoclonal antibody, Western Blot analysis of AKR1B10 expression in HepG2.)
Application Data (Detection limit for recombinant GST tagged AKR1B10 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKR1B10 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKR1B10 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of AKR1B10 expression in transfected 293T cell line by AKR1B10 monoclonal antibody. Lane 1: AKR1B10 transfected lysate (36kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (33.59kD).)
Application Data (Detection limit for recombinant GST tagged NR3C1 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NR3C1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of NR3C1 expression in transfected 293T cell line by NR3C1 monoclonal antibody. Lane 1: NR3C1 transfected lysate (85.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NR3C1 monoclonal antibody, Western Blot analysis of NR3C1 expression in HeLa NE.)
WB (Western Blot) (NR3C1 monoclonal antibody. Western Blot analysis of NR3C1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.2 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.2 ug/ml])
Application Data (Detection limit for recombinant GST tagged TAF11 is approximately 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TAF11 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TAF11 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (TAF11 monoclonal antibody (M06), clone 3G6 Western Blot analysis of TAF11 expression in Hela S3 NE.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H2AX-S139 antibody . Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Phospho-Histone H2AX-S139 antibody . Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Phospho-Histone H2AX-S139 antibody . Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using Phospho-Histone H2AX-S139 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal using Phospho-Histone H2AX-S139 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using Phospho-Histone H2AX-S139 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H2AX-S139 antibody at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
ICC (Immunocytochemistry) (ICC staining Tissue Factor in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Tissue Factor in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Tissue Factor in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Tissue Factor antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Tissue Factor antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Tissue Factor antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Tissue Factor on different lysates using anti-Tissue Factor antibody at 1/1, 000 dilution. Positive control: Lane 1: U937 Lane 2: SH-SY-5Y Lane 3: Mouse brain)
WB (Western Blot) (Detection of mouse hSET1 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody [AAA23799 hSET1] (AAA23799 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot) (Detection of human hSET1 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, K-562, U2OS, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody [AAA23799 hSET1] (AAA23799 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation) (Detection of human hSET1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-hSET1 recombinant monoclonal antibody [BL-178-12C7] (AAA23799 lot 2) used for IP at 20 ul/mg lysate. hSET1 was also immunoprecipitated by a previous lot of this antibody (AAA23799 lot 1) and a second antibody against a different epitope of hSET1 . For blotting immunoprecipitated hSET1, AAA23799 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry) (Detection of human hSET1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody (AAA23799) used at 1:25. Secondary: DyLight 594-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry) (Detection of human hSET1 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody (AAA23799 Lot 2) used at 1:25. Detection: DAB)
IHC (Immunohistochemistry) (Detection of human hSET1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody (AAA23799 Lot 2) used at 1:25. Detection: DAB)
ICC (Immunocytochemistry) (Detection of human hSET1 by immunocytochemistry. Sample: FFPE section of human jurkat cells. Antibody: Rabbit anti-hSET1 recombinant monoclonal antibody (AAA23799 Lot 2) used at 1:25. Detection: DAB)
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF)
Purity
Purified
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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