Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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Application Data (Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)

Application Data (Published customer image: Western blot analysis to determine type of activation of adult primary microglial cells produced from wild-type and IL-1 KO mice stimulated with IL-4, IL-13 or IL-4/IL-13 with or without IL-1beta. (A) Representative western blotting data of primary microglial cells produced from wild-type (wild) and IL-1 KO mice and exposed for 24 hours to IL-4, IL-13 or IL-4 plus IL-13 (IL-4/IL-13) with or without IL-1beta. Each lane expected to CD206 blotting were applied 7 ug of reduced samples. Non-reduced samples (5 ug) were applied to detect CD206. Densitometric analysis of COX2 (B), Ym1 (C), Arg-1 (D) and CD206 (E) (n = 3 each group). (B) COX2 levels are increased by exposure of cells to IL-1beta and are not influenced by IL-4 or IL-13 alone. The COX2 level was slightly enhanced by IL-1beta and IL-4 co-treatment. (C) Ym1 levels are increased by exposure of cells to IL-4 and IL-4/Il-13 and are synergistically increased by co-treatment with IL-1beta. However, only a low level of Ym1 is seen upon exposure of cells to IL-13, and is significantly less than that seen in response to exposure of cells to IL-4. (D) Arg-1 shows similar levels in response to exposure to IL-4 and IL-4/IL-13; these are synergistically increased by co-treatment with IL-1beta. However, low levels of Arg-1 are seen for exposure of cells to IL-13. (G) CD206 was detected in response to exposure of cells to both IL-4 and IL-4/IL-13 with or without IL-1beta; however, CD206 levels in IL-13-exposed samples were lower than those seen with the other treatments. Data are expressed as mean +/- SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the IL-4-treated group without IL-1beta for each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX 2, cyclooxygenase 2.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation. 2012 Apr 7;9:65.)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)

Application Data (Staining of J774 cell line with Rat anti Mouse CD206 following permeabilisation with Leucoperm)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 488)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 647)

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CD206, Monoclonal Antibody (Cat# AAA12119)

Full Name

RAT ANTI MOUSE CD206:FITC

Gene Names

Mrc1; MR; CD206; AW259686

Applications

Flow cytometry (FC/FACS)*

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,980/4x0.1 mg $2,195/5x0.1 mg

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Application Data (Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)

Application Data (Published customer image: Western blot analysis to determine type of activation of adult primary microglial cells produced from wild-type and IL-1 KO mice stimulated with IL-4, IL-13 or IL-4/IL-13 with or without IL-1beta. (A) Representative western blotting data of primary microglial cells produced from wild-type (wild) and IL-1 KO mice and exposed for 24 hours to IL-4, IL-13 or IL-4 plus IL-13 (IL-4/IL-13) with or without IL-1beta. Each lane expected to CD206 blotting were applied 7 ug of reduced samples. Non-reduced samples (5 ug) were applied to detect CD206. Densitometric analysis of COX2 (B), Ym1 (C), Arg-1 (D) and CD206 (E) (n = 3 each group). (B) COX2 levels are increased by exposure of cells to IL-1beta and are not influenced by IL-4 or IL-13 alone. The COX2 level was slightly enhanced by IL-1beta and IL-4 co-treatment. (C) Ym1 levels are increased by exposure of cells to IL-4 and IL-4/Il-13 and are synergistically increased by co-treatment with IL-1beta. However, only a low level of Ym1 is seen upon exposure of cells to IL-13, and is significantly less than that seen in response to exposure of cells to IL-4. (D) Arg-1 shows similar levels in response to exposure to IL-4 and IL-4/IL-13; these are synergistically increased by co-treatment with IL-1beta. However, low levels of Arg-1 are seen for exposure of cells to IL-13. (G) CD206 was detected in response to exposure of cells to both IL-4 and IL-4/IL-13 with or without IL-1beta; however, CD206 levels in IL-13-exposed samples were lower than those seen with the other treatments. Data are expressed as mean +/- SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the IL-4-treated group without IL-1beta for each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX 2, cyclooxygenase 2.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation. 2012 Apr 7;9:65.)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)

Application Data (Staining of J774 cell line with Rat anti Mouse CD206 following permeabilisation with Leucoperm)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 488)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 647)

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CD206, Monoclonal Antibody (Cat# AAA12124)

Full Name

RAT ANTI MOUSE CD206:RPE

Gene Names

Mrc1; MR; CD206; AW259686

Applications

Flow cytometry (FC/FACS)*

Pricing

$645/100 Tests $1,245/2x100 Tests $1,815/3x100 Tests $2,350/4x100 Tests $2,650/5x100 Tests

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Application Data (Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p)

Application Data (Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012) Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis. PLoS ONE 7(12): e51608.)

Application Data (Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp)

Application Data (Published customer image: Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 um for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). Scale bar was 40 um for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean +/-S.E. The p values were from Student's t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014) Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9(12): e116023.)

Application Data (Published customer image: Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. Histograms show receptor expression profile of CX3CR1hiLYVE-1- (green line) and CX3CR1loLYVE-1+ (red line) macrophages. (B) Sorted CX3CR1loLYVE-1+ and CX3CR1hiLYVE-1- macrophages spun onto glass slides and stained with Wright's stain. 1000x magnification. (C) mRNA expression profile of chemokine receptors, chemokines and VEGFs in pancreatic macrophage subsets. mRNA levels examined by qRT-PCR in duplicate or triplicate. 6 -7 mice/group, data representative of 2 -4 separate experiments.From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al. (2011) Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes. PLoS ONE 6(11): e28023.)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa Fluor 488 . following permeablisation with Leucoperm)

Application Data (Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Medium power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Low power)

Application Data (Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa Fluor 647 . following permeablisation with Leucoperm)

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CD68, Monoclonal Antibody (Cat# AAA12105)

Full Name

RAT ANTI MOUSE CD68:FITC

Gene Names

Cd68; Lamp4; gp110; Scard1

Applications

Flow cytometry (FC/FACS)*

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,980/4x0.1 mg $2,195/5x0.1 mg

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Application Data (Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. High power)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD8 Alpha: Alexa Fluor 488)

Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: FITC)

Application Data (Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. Medium power)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha: Biotin)

Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE - Alexa Fluor 647)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha:RPE)

Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE)

Application Data (Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. Low power)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8)

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CD8 ALPHA, Monoclonal Antibody (Cat# AAA12000)

Full Name

RAT ANTI MOUSE CD8 ALPHA

Gene Names

Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunofluorescence (IF)

Pricing

$410/0.1 mg $790/2x0.1 mg $1,145/3x0.1 mg $1,480/4x0.1 mg $1,600/5x0.1 mg

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Application Data (Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. High power)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD8 Alpha: Alexa Fluor 488)

Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: FITC)

Application Data (Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. Medium power)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha: Biotin)

Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE - Alexa Fluor 647)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha:RPE)

Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE)

Application Data (Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. Low power)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8)

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CD8 ALPHA, Monoclonal Antibody (Cat# AAA12180)

Full Name

RAT ANTI MOUSE CD8 ALPHA

Gene Names

Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunofluorescence (IF)

Pricing

$250/0.025 mg $475/2x0.025 mg $685/3x0.025 mg $870/5x0.025 mg $885/4x0.025 mg

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Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 488)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Low Endotoxin)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . Medium power)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD204:FITC)

Application Data (Western Blot staining of J774 lysate (non reduced) probed with Rat anti Mouse CD204)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Biotin)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . High power)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 647)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204:FITC)

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CD204, Monoclonal Antibody (Cat# AAA12080)

Full Name

RAT ANTI MOUSE CD204

Gene Names

Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1

Applications

Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)*

Pricing

$250/0.025 mg $475/2x0.025 mg $685/3x0.025 mg $870/5x0.025 mg $885/4x0.025 mg

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Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 488 (AAA12072A488))

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Low Endotoxin)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . Medium power)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD204:FITC)

Application Data (Western Blot staining of J774 lysate (non reduced) probed with Rat anti Mouse CD204)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Biotin)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . High power)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 647 (AAA12072A647))

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204:FITC)

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CD204, Monoclonal Antibody (Cat# AAA12072)

Full Name

RAT ANTI MOUSE CD204

Gene Names

Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1

Reactivity

Channel catfish, Pig

Applications

Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)*

Pricing

$560/0.25 mg $1,080/2x0.25 mg $1,570/3x0.25 mg $2,035/4x0.25 mg $2,275/5x0.25 mg

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Application Data (Staining of mouse spleen cells with Rat anti Mouse CD4)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4: Alexa Fluor 405)

Application Data (Staining of mouse spleen with Rat anti Mouse CD4: Low Endotoxin)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD4:RPE)

Application Data (Staining of mouse spleen with Rat anti Mouse CD4)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD4: APC)

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CD4, Monoclonal Antibody (Cat# AAA11939)

Full Name

RAT ANTI MOUSE CD4

Gene Names

Cd4; L3T4; Ly-4

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS)

Pricing

$410/0.1 mg $790/2x0.1 mg $1,145/3x0.1 mg $1,480/4x0.1 mg $1,600/5x0.1 mg

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Application Data (Published customer image: Histological staining of control and HBOT wounds at post-wounding days 7 and 29. A -D) H&E staining. E -H) CD34 immunohistochemistry. I+J) CD68 immunohistochemistry.From: uk B, Tong M, Fijneman EMG, van Neck JW (2014) Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats. PLoS ONE 9(10): e108533.)

Application Data (Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:RPE . Permeabilised with Leucoperm (Fix & Perm))

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Medium power)

Application Data (Published customer image: Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 um.From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014) Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats. PLoS ONE 9(12): e114694.)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)

Application Data (Published customer image: Western blotting of ED1 (Marker of activated microglia) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). Brains were treated with PBS or rAAV2/IL12 and then implanted with tumor on the last day of Week 2 post viral vector injection. The brains were used for Western blotting analysis of the expression of ED1, 34 kDa, and TRAIL, 104 kDa, from microglial cells on the last day of week 1, 2, and 3 following tumor implantation. The brain slices for Western blotting analysis are shown at the bottom. All brains were harvested on the last day of week 1, week 2, and week 3 post tumor implantation. The scale bars indicate 2 mm in brain sections.From: The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. Chiu TL, Wang MJ, Su CC. J Biomed Sci. 2012 Apr 22;19:45. doi: 10.1186/1423-0127-19-45.)

Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 647 following permeabilisation with Leucoperm)

Application Data (Published customer image: Immunohistochemistry of ED1 stain in brain sections treated with rAAV2/IL12 (A, B, C, D, n = 4), AAV2/GFP (E, F, n = 2), or PBS (G, H, n = 2), accompanied with tumor implantation; and that in the brain section treated with nothing (n = 1). Immunohistochemistry of brain sections was performed for ED1 on the last day of week 3 after tumor implantation. The brain sections were stained with hematoxylin for nuclei and ED1 for activated microglial cells. The 1st column shows the brain sections pictured before immunostaining; the 2nd column shows the brain sections pictured after staining; the 3rd to 6th columns show pictures taken at four quadrants (black squares in 2nd column) of the tumor adjacent to the normal tissue in the right hemisphere. ED1-positive cells show dark brown. The scale bars indicate 2 mm in 1st and 2nd columns and 100 um in 3rd-6th columns.From: Chiu TL, Wang MJ, Su CC. The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. J Biomed Sci. 2012 Apr 22;19:45.)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power)

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CD68, Monoclonal Antibody (Cat# AAA12149)

Full Name

MOUSE ANTI RAT CD68

Applications

Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin*

Pricing

$520/0.1 mg $1,000/2x0.1 mg $1,455/3x0.1 mg $1,885/4x0.1 mg $2,080/5x0.1 mg

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Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 647 (AAA12013A647))

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human HLA ABC:Biotin)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 488 (AAA12013A488))

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:FITC)

Application Data (Staining of peripheral blood lymphocytes with Mouse anti Human HLA-ABC: Low Endotoxin)

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HLA ABC, Monoclonal Antibody (Cat# AAA12013)

Full Name

MOUSE ANTI HUMAN HLA ABC

Reactivity

Baboon, Bovine, Chimpanzee, Cynomolgus monkey, Gorilla, Macaque, Rhesus Monkey, Shrew

Applications

Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Pricing

$560/0.2 mg $1,080/2x0.2 mg $1,570/3x0.2 mg $2,035/4x0.2 mg $2,275/5x0.2 mg

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Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Biotin)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:RPE)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Azide Free)

Application Data (Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 followed by the Histar detection system . Low power)

Application Data (Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 followed by the Histar detection system . High power)

Application Data (Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 followed by the Histar detection system . Low power)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Alexa Fluor 647 (AAA11991A647))

Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44, red in A and Mousse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44, red in A and Mousse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. medium power)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:FITC)

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CD11b, Monoclonal Antibody (Cat# AAA11991)

Full Name

MOUSE ANTI HUMAN CD11b

Gene Names

ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6

Reactivity

Baboon, Cynomolgus monkey, Rhesus Monkey

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunoprecipitation (IP)

Pricing

$560/0.2 mg $1,080/2x0.2 mg $1,570/3x0.2 mg $2,035/4x0.2 mg $2,275/5x0.2 mg

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WB (Western Blot) (Western blot analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFIC on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody.Lane 1: NFIC transfected lysate (47.6kD).Lane 2: Non-transfected lysate.)

WB (Western Blot) (NFIC monoclonal antibody Western Blot analysis of NFIC expression in A-431.)

WB (Western Blot) (Western Blot detection against Immunogen (72.82kD).)

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Nuclear Factor I/C, Monoclonal Antibody (Cat# AAA24060)

Full Name

Nuclear Factor I/C (NFIC, CCAAT-binding Transcription Factor, CTF, Nuclear Factor 1 C-Type, CTF5, MGC20153, NF-I, NF1-C, CTF, TGGCA-Binding Protein, NFI)

Gene Names

NFATC2; NFAT1; NFATP

Reactivity

Human

Applications

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$770/0.1 mg $1,490/2x0.1 mg $2,170/3x0.1 mg $2,810/4x0.1 mg $3,235/5x0.1 mg

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Application Data (Detection limit for recombinant GST tagged HIP1 is ~0.3ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of HIP1 transfected lysate using HIP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HIP1 rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HIP1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of HIP1 expression in transfected 293T cell line by HIP1 monoclonal antibody Lane 1: HIP1 transfected lysate (116.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (HIP1 monoclonal antibody Western Blot analysis of HIP1 expression in HeLa)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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HIP1, Monoclonal Antibody (Cat# AAA24828)

Full Name

HIP1 (Huntingtin-interacting Protein 1, Huntingtin-interacting Protein I, MGC126506) (Biotin)

Gene Names

HIP1; SHON; HIP-I; ILWEQ; SHONbeta; SHONgamma

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with Huntingtin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Huntingtin on A431 cell using anti-Huntingtin antibody at 1/500 dilution.)

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Huntingtin, Monoclonal Antibody (Cat# AAA30460)

Full Name

Huntingtin Antibody

Gene Names

HTT; HD; IT15

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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WB (Western Blot) (Western blot analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged PIP5K3 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PIP5K3 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PIP5K3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody. Lane 1: PIP5K3 transfected lysate (50.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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PIP5K3, Monoclonal Antibody (Cat# AAA24317)

Full Name

PIP5K3 (PIKFYVE, 1-phosphatidylinositol 3-phosphate 5-kinase, Phosphatidylinositol 3-phosphate 5-kinase, FYVE Finger-containing Phosphoinositide Kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase Type III, PIPkin-III, Type III PIP Kinase, KIAA0981

Gene Names

PIKFYVE; CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Proximity Ligation Analysis of protein-protein interactions between MSH2 and MLH1 HeLa cells were stained with anti-MSH2 rabbit purified polyclonal 1:1200 and anti-MLH1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged MLH1 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MLH1 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MLH1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1ug/ml])

WB (Western Blot) (MLH1 monoclonal antibody Western Blot analysis of MLH1 expression in HeLa NE.)

WB (Western Blot) (Western Blot detection against Immunogen (108.9kD).)

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MLH1, Monoclonal Antibody (Cat# AAA24573)

Full Name

MLH1 (MutL Homolog 1, MutL Protein Homolog 1, COCA2, DNA Mismatch Repair Protein Mlh1, FCC2, hMLH1, HNPCC, HNPCC2, MGC5172, MutL Homolog 1 Colon Cancer Nonpolyposis Type 2) APC

Gene Names

MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (EHD3 monoclonal antibody. Western Blot analysis of EHD3 expression in COLO 320 HSR.)

WB (Western Blot) (EHD3 monoclonal antibody, Western Blot analysis of EHD3 expression in IMR-32.)

Application Data (Detection limit for recombinant GST tagged EHD3 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EHD3 on HeLa cell. [antibody concentration 25ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EHD3 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)

WB (Western Blot) (EHD3 monoclonal antibody. Western Blot analysis of EHD3 expression in MCF-7.)

WB (Western Blot) (Western Blot detection against Immunogen (31.61kD).)

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EHD3, Monoclonal Antibody (Cat# AAA25085)

Full Name

EHD3 (EH Domain-containing Protein 3, PAST Homolog 3, EHD2, PAST3) (FITC)

Gene Names

EHD3; PAST3

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (USF2 monoclonal antibody (M03), clone 6A9. Western Blot analysis of USF2 expression in PC-12 (Cat # L012V1).)

Application Data (Detection limit for recombinant GST tagged USF2 is approximately 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USF2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USF2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (USF2 monoclonal antibody (M03), clone 6A9. Western Blot analysis of USF2 expression in different cell lines.)

WB (Western Blot) (USF2 monoclonal antibody (M03), clone 6A9. Western Blot analysis of USF2 expression in Hela S3 NE (Cat # L013V3).)

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USF2, Monoclonal Antibody (Cat# AAA26109)

Full Name

USF2 (Upstream Transcription Factor 2, c-fos Interacting, FIP, bHLHb12) (APC)

Gene Names

USF2; FIP; bHLHb12

Applications

Immunofluorescence (IF), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (CDK4 monoclonal antibody (M09), clone 8A10. Western Blot analysis of CDK4 expression in Raw 264.7 (Cat # L024V1).)

WB (Western Blot) (CDK4 monoclonal antibody (M09), clone 8A10. Western Blot analysis of CDK4 expression in NIH/3T3 (Cat # L018V1).)

Application Data (Detection limit for recombinant GST tagged CDK4 is approximately 3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDK4 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (CDK4 monoclonal antibody (M09), clone 8A10 Western Blot analysis of CDK4 expression in HeLa (Cat # L013V1).)

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CDK4, Monoclonal Antibody (Cat# AAA26365)

Full Name

CDK4 (Cyclin-Dependent Kinase 4, CMM3, MGC14458, PSK-J3) (FITC)

Gene Names

CDK4; CMM3; PSK-J3

Applications

Immunofluorescence (IF), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with KAP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining KAP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-KAP1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-KAP1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-KAP1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-KAP1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-KAP1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-KAP1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of KAP1 on different lysates using anti-KAP1 antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: Hela)

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KAP1, Monoclonal Antibody (Cat# AAA30205)

Full Name

KAP1 Antibody

Gene Names

TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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WB (Western Blot) (Western blot analysis of MRPL12 over-expressed 293 cell line, cotransfected with MRPL12 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MRPL12 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged MRPL12 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MRPL12 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MRPL12 on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody. Lane 1: MRPL12 transfected lysate (21.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (MRPL12 monoclonal antibody, Western Blot analysis of MRPL12 expression in COLO 320 HSR.)

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MRPL12, Monoclonal Antibody (Cat# AAA24575)

Full Name

MRPL12 (Mitochondrial Ribosomal Protein L12, 39S Ribosomal Protein L12, Mitochondrial, 5c5-2, FLJ60124, L12mt, MGC8610, MRP-L12, MRP-L31/34, MRPL7, MRPL7/L12, RPML12) APC

Gene Names

MRPL12; 5c5-2; L12mt; MRPL7; RPML12; MRPL7/L12; MRP-L31/34

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining STAT5b in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining STAT5b in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining STAT5b in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-STAT5b antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-STAT5b antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-STAT5b antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-STAT5b antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of STAT5b on different lysates using anti-STAT5b antibody at 1/1, 000 dilution. Positive control: Lane 1: K562 Lane 2: Hela)

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STAT5b, Monoclonal Antibody (Cat# AAA30207)

Full Name

STAT5b Antibody

Gene Names

STAT5B; STAT5

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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IP (Immunoprecipitation) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)

ELISA (Figure 5. ELISA plate was coated with recombinant BCMA-hFc fusion protein (PME100001), followed by pre-blocking with huC11D5.3 antibody (Grey bar) or rabbit control IgG (Black bar), and then different rabbit DimAbs antibodies were added to check the competitive inhibition of huC11D5.3. DM3 clone exhibits the strongest inhibition (Red bar). This data indicated that DM3 bind to the same epitope as bb2121.)

Application Data (Figure 4. Affinity ranking of different DimAb clones by titration of rabbit DimAb antibody concentration onto K562-BCMA or NCI-H929 cells. Different concentrations of various anti-BCMA DimAb clones were incubated with K562-BCMA (A) or NCI-H929 cells (B) at 4 degree C. Bound rabbit IgG was detected in flow cytometry analysis. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Application Data (Figure 3. Phylogenetic analysis of different Anti-BCMA DimAb clones. A) heavy chain and B) Light chain.)

Application Data

FCM (Flow Cytometry) (Figure 2. A. Flow cytometry analysis with anti-BCMA (DM6) on NCI-H929 cells (Red histogram) or rabbit control antibody on NCI-H929 cells (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM6) on NCI-H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Application Data

FCM (Flow Cytometry) (Figure 1. A. Flow cytometry analysis with anti-BCMA (DM6) on K562-BCMA (Red histogram)(K562 cells stably transduced by human BCMA full length gene) and K562 (Negative control cell line)(Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM6). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

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BCMA, Monoclonal Antibody (Cat# AAA30719)

Full Name

Anti-BCMA antibody (DM6), Rabbit mAb

Reactivity

Human

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Purity

Purified from cell culture supernatant by affinity chromatography

Pricing

$230/0.01 mg $840/0.1 mg $1,625/2x0.1 mg $2,370/3x0.1 mg $3,070/4x0.1 mg $3,550/5x0.1 mg

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Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 647)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human HLA ABC:Biotin)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:Alexa Fluor 488)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human HLA ABC:FITC)

Application Data (Staining of peripheral blood lymphocytes with Mouse anti Human HLA-ABC: Low Endotoxin)

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HLA ABC, Monoclonal Antibody (Cat# AAA11895)

Full Name

MOUSE ANTI HUMAN HLA ABC:HRP

Reactivity

Macaque, Bovine, Cynomolgus Monkey, Baboon, Rhesus Monkey, Chimpanzee, Gorilla, Shrew

Applications

Immunohistology Frozen*

Pricing

$495/0.1 mg $955/2x0.1 mg $1,385/3x0.1 mg $1,795/4x0.1 mg $1,975/5x0.1 mg

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Application Data (Frozen mouse spleen stained with Rat anti Mouse CD169)

Application Data (Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Application Data (Mouse peritoneal macrophages stained with Rat anti Mouse CD169:FITC)

Application Data (Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Medium power)

Application Data (Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. High power)

Application Data (Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Low power)

Application Data (Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

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CD169, Monoclonal Antibody (Cat# AAA12223)

Full Name

RAT ANTI MOUSE CD169:Low Endotoxin

Gene Names

Siglec1; Sn; Cd169; Siglec-1

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunofluorescence (IF)

Pricing

$960/0.5 mg $1,860/2x0.5 mg $2,710/3x0.5 mg $3,515/4x0.5 mg $4,055/5x0.5 mg

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Application Data (Published customer image: Histological staining of control and HBOT wounds at post-wounding days 7 and 29. A -D) H&E staining. E -H) CD34 immunohistochemistry. I+J) CD68 immunohistochemistry.From: uk B, Tong M, Fijneman EMG, van Neck JW (2014) Hyperbaric Oxygen Therapy to Treat Diabetes Impaired Wound Healing in Rats. PLoS ONE 9(10): e108533.)

Application Data (Staining of rat peritoneal macrophages cells with Mouse anti Rat CD68:RPE . Permeabilised with Leucoperm (Fix & Perm))

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Medium power)

Application Data (Published customer image: Immunohistochemical stains of ED1 and TRAIL in brain sections treated with AAV2/IL1. Immunohistochemistry for CD68 in the optic nerve. There is a cellular accumulation indicated by DAPI at the crushed sites of the optic nerve. Some of the cells are positively stained with CD68 suggesting that there is a recruitment of microglia/macrophages to the crushed sites. Bar = 100 um.From: Suzuki H, Oku H, Horie T, Morishita S, Tonari M, et al. (2014) Changes in Expression of Aquaporin-4 and Aquaporin-9 in Optic Nerve after Crushing in Rats. PLoS ONE 9(12): e114694.)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)

Application Data (Published customer image: Western blotting of ED1 (Marker of activated microglia) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). Brains were treated with PBS or rAAV2/IL12 and then implanted with tumor on the last day of Week 2 post viral vector injection. The brains were used for Western blotting analysis of the expression of ED1, 34 kDa, and TRAIL, 104 kDa, from microglial cells on the last day of week 1, 2, and 3 following tumor implantation. The brain slices for Western blotting analysis are shown at the bottom. All brains were harvested on the last day of week 1, week 2, and week 3 post tumor implantation. The scale bars indicate 2 mm in brain sections.From: The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. Chiu TL, Wang MJ, Su CC. J Biomed Sci. 2012 Apr 22;19:45. doi: 10.1186/1423-0127-19-45.)

Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD68: Alexa Fluor 647 following permeabilisation with Leucoperm)

Application Data (Published customer image: Immunohistochemistry of ED1 stain in brain sections treated with rAAV2/IL12 (A, B, C, D, n = 4), AAV2/GFP (E, F, n = 2), or PBS (G, H, n = 2), accompanied with tumor implantation; and that in the brain section treated with nothing (n = 1). Immunohistochemistry of brain sections was performed for ED1 on the last day of week 3 after tumor implantation. The brain sections were stained with hematoxylin for nuclei and ED1 for activated microglial cells. The 1st column shows the brain sections pictured before immunostaining; the 2nd column shows the brain sections pictured after staining; the 3rd to 6th columns show pictures taken at four quadrants (black squares in 2nd column) of the tumor adjacent to the normal tissue in the right hemisphere. ED1-positive cells show dark brown. The scale bars indicate 2 mm in 1st and 2nd columns and 100 um in 3rd-6th columns.From: Chiu TL, Wang MJ, Su CC. The treatment of glioblastoma multiforme through activation of microglia and TRAIL induced by rAAV2-mediated IL-12 in a syngeneic rat model. J Biomed Sci. 2012 Apr 22;19:45.)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. High power)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD68 antibody , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counter-stained blue using DAPI. Low power)

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CD68, Monoclonal Antibody (Cat# AAA12147)

Full Name

MOUSE ANTI RAT CD68:Biotin

Applications

Flow cytometry (FC/FACS)*

Pricing

$665/100 Tests $1,285/2x100 Tests $1,870/3x100 Tests $2,420/4x100 Tests $2,750/5x100 Tests

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WB (Western Blot) (SSH3 monoclonal antibody, Western Blot analysis of SSH3 expression in A-431.)

Application Data (Detection limit for recombinant GST tagged SSH3 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SSH3 on A-431 cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SSH3 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 1-10ug/ml])

WB (Western Blot) (Western Blot analysis of SSH3 expression in transfected 293T cell line by SSH3 monoclonal antibody. Lane 1: SSH3 transfected lysate (73kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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SSH3, Monoclonal Antibody (Cat# AAA25854)

Full Name

SSH3 (Protein Phosphatase Slingshot Homolog 3, SSH-like Protein 3, SSH3L, SSH-3L, hSSH-3L) (PE)

Gene Names

SSH3; SSH3L

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (CAND1 monoclonal antibody (M04), clone 4D10. Western Blot analysis of CAND1 expression in Raw 264.7.)

WB (Western Blot) (CAND1 monoclonal antibody (M04), clone 4D10. Western Blot analysis of CAND1 expression in PC-12.)

WB (Western Blot) (CAND1 monoclonal antibody (M04), clone 4D10. Western Blot analysis of CAND1 expression in NIH/3T3.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAND1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAND1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (CAND1 monoclonal antibody (M04), clone 4D10 Western Blot analysis of CAND1 expression in Hela S3 NE.)

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CAND1, Monoclonal Antibody (Cat# AAA26366)

Full Name

CAND1 (Cullin-Associated and Neddylation-dissociated 1, DKFZp434M1414, FLJ10114, FLJ10929, FLJ38691, FLJ90441, KIAA0829, TIP120, TIP120A) (FITC)

Gene Names

CAND1; TIP120; TIP120A

Applications

ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with MEK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining MEK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MEK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MEK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-MEK2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MEK2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEK2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MEK2 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of MEK2 on different lysates using anti-MEK2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: 293T)

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MEK2, Monoclonal Antibody (Cat# AAA30206)

Full Name

MEK2 Antibody

Gene Names

MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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Application Data (Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD4 . Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD4 and StarBright Violet 610 conjugated Rat anti Mouse CD3 . All experiments performed on red blood lys)

Application Data (Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD3 . Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD3 and SBV440 conjugated Rat anti Mouse CD4 . All experiments performed on mouse blood gated on live sin)

Application Data (Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD3 . Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD3 and SBV515 conjugated Rat anti Mouse CD4 . All experiments performed on red cell lysed mouse blood ga)

Application Data (Figure A. FITC conjugated Rat anti Mouse CD4 . Figure B. FITC conjugated Rat anti Mouse CD4 and SBV515 conjugated Rat anti Mouse CD3 . All experiments performed on red cell lysed mouse blood gated on live single cell lym)

Application Data (Figure A. Alexa Fluor 647 conjugated Rat anti Mouse CD3 and FITC conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 647 conjugated Rat anti Mouse CD3 and FITC conjugated Rat anti Mouse CD4 . All expe)

Application Data (Figure A. RPE conjugated Rat anti Mouse CD3 and Alexa Fluor 647 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD3 and Alexa Fluor 647 conjugated Rat anti Mouse CD4 . All expe)

Application Data (Figure A. RPE conjugated Rat anti Mouse CD3 and Alexa Fluor 488 conjugated Rat IgG2a isotype control . Figure B. PE conjugated Rat anti Mouse CD3 and Alexa Fluor 488 conjugated Rat anti Mouse CD4 . All exper)

Application Data (Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD3 and Alexa Fluor 647 conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 and Alexa Fluor 647 conjugated Rat anti Mou)

Application Data (Figure A. Pacific Blue conjugated Rat anti Mouse CD3 and RPE conjugated Rat IgG2a isotype control . Figure B. Pacific Blue conjugated Rat anti Mouse CD3 and RPE conjugated Rat anti Mouse CD4 . All experiments pe)

Application Data (Figure A. Alexa Fluor 700 conjugated Rat anti Mouse CD3 and FITC conjugated Rat IgG2a isotype control . Figure B. Alexa Fluor 700 conjugated Rat anti Mouse CD3 and FITC conjugated Rat anti Mouse CD4 . All expe)

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CD4, Monoclonal Antibody (Cat# AAA12287)

Full Name

Rat anti Mouse CD4:Amethyst Orange

Gene Names

Cd4; L3T4; Ly-4

Reactivity

Mouse

Applications

ELISA (EIA)

Purity

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Pricing

$585/0.1 mg $1,130/2x0.1 mg $1,640/3x0.1 mg $2,125/4x0.1 mg $2,380/5x0.1 mg

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of DHODH using anti-DHODH antibody (AAA19712).DHODH was detected in a paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-DHODH Antibody (AAA19712) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of DHODH using anti-DHODH antibody (AAA19712).DHODH was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-DHODH Antibody (AAA19712) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of DHODH using anti-DHODH antibody (AAA19712).DHODH was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-DHODH Antibody (AAA19712) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of DHODH using anti-DHODH antibody (AAA19712).DHODH was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-DHODH Antibody (AAA19712) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of DHODH using anti-DHODH antibody (AAA19712).DHODH was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-DHODH Antibody (AAA19712) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of DHODH using anti-DHODH antibody (AAA19712).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human A431 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat testis tissue lysates,Lane 6: rat liver tissue lysates,Lane 7: mouse testis tissue lysates,Lane 8: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DHODH antigen affinity purified monoclonal antibody (#AAA19712) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DHODH at approximately 43 kDa. The expected band size for DHODH is at 43 kDa.)

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DHODH, Monoclonal Antibody (Cat# AAA19712)

Full Name

Anti-DHODH Antibody Picoband (monoclonal, 2G7)

Gene Names

DHODH; URA1; POADS; DHOdehase

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of JK cells using anti-ERLIN2 antibody (AAA19715).Overlay histogram showing JK cells stained with AAA19715 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ERLIN2 Antibody (AAA19715, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of ERLIN2 using anti-ERLIN2 antibody (AAA19715).ERLIN2 was detected in an immunocytochemical section of T47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-ERLIN2 Antibody (AAA19715) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of ERLIN2 using anti-ERLIN2 antibody (AAA19715).ERLIN2 was detected in a paraffin-embedded section of human thyroiditis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-ERLIN2 Antibody (AAA19715) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of ERLIN2 using anti-ERLIN2 antibody (AAA19715).ERLIN2 was detected in a paraffin-embedded section of human squamous cell lung carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-ERLIN2 Antibody (AAA19715) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of ERLIN2 using anti-ERLIN2 antibody (AAA19715).ERLIN2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-ERLIN2 Antibody (AAA19715) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of ERLIN2 using anti-ERLIN2 antibody (AAA19715).ERLIN2 was detected in a paraffin-embedded section of human ovarian serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-ERLIN2 Antibody (AAA19715) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of ERLIN2 using anti-ERLIN2 antibody (AAA19715).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human T47D whole cell lysates,Lane 3: human HCCT tissue lysates,Lane 4: human HCCP tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ERLIN2 antigen affinity purified monoclonal antibody (#AAA19715) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ERLIN2 at approximately 43 kDa. The expected band size for ERLIN2 is at 38 kDa.)

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Erlin-2/ERLIN2, Monoclonal Antibody (Cat# AAA19715)

Full Name

Anti-Erlin-2/ERLIN2 Antibody Picoband (monoclonal, 3H9A2)

Gene Names

ERLIN2; NET32; SPFH2; SPG18; C8orf2; Erlin-2

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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WB (Western Blot) (Figure 11. Western blot analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat heart tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: rat lung tissue lysates,Lane 4: mouse heart tissue lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse lung tissue lysates,Lane 7: rat RH35 whole cell lysates,Lane 8: mouse HEPA1-6 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cyclophilin E/PPIE antigen affinity purified monoclonal antibody (#AAA19717) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyclophilin E/PPIE at approximately 35 kDa. The expected band size for Cyclophilin E/PPIE is at 35 kDa.)

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of U937 cells using anti-Cyclophilin E/PPIE antibody (AAA19717).Overlay histogram showing U937 cells stained with AAA19717 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cyclophilin E/PPIE Antibody (AAA19717, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in an immunocytochemical section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The tissue section was developed using Phalloidin-iFluor 488 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Cyclophilin E/PPIE was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cyclophilin E/PPIE Antibody (AAA19717) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (AAA19717).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Lane 4: human Caco-2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cyclophilin E/PPIE antigen affinity purified monoclonal antibody (#AAA19717) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyclophilin E/PPIE at approximately 35 kDa. The expected band size for Cyclophilin E/PPIE is at 35 kDa.)

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Cyclophilin E/PPIE, Monoclonal Antibody (Cat# AAA19717)

Full Name

Anti-Cyclophilin E/PPIE Antibody Picoband (monoclonal, 4I9)

Gene Names

PPIE; CYP33; CYP-33

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Immunogen affinity purified.

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of K562 cells using anti-D2AP antibody (M01756). Overlay histogram showing K562 cells stained with M01756 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-D2AP Antibody (M01756, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of CD2AP using anti-CD2AP antibody (M01756). CD2AP was detected in paraffin-embedded section of human mammary cancer. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-CD2AP Antibody (M01756) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of CD2AP using anti-CD2AP antibody (M01756). CD2AP was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-CD2AP Antibody (M01756) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of CD2AP using anti-CD2AP antibody (M01756). CD2AP was detected in paraffin-embedded section of human colon cancer. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epi1ope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-CD2AP Antibody (M01756) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of CD2AP using anti-CD2AP antibody (M01756). CD2AP was detected in paraffin-embedded section of human colon cancer. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epi1ope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-CD2AP Antibody (M01756) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of D2AP using anti-D2AP antibody (M01756). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysate, Lane 2: human A431 whole cell lysate, Lane 3: human 293T whole cell lysate, Lane 4: human U20S whole cell lysate, Lane 5: human HL-60 whole cell lysate, Lane 6: human MCF-7 whole cell lysate, Lane 7: human Hela whole cell lysate, Lane 8: human PANC-1 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-D2AP antigen affinity purified monoclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.)

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CD2AP, Monoclonal Antibody (Cat# AAA19185)

Full Name

Anti-CD2AP Antibody (monoclonal, 5F8)

Gene Names

CD2AP; CMS

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS)

Purity

Immunogen Affinity Purified

Pricing

$220/0.01 mg $480/0.1 mg $480/0.1 mg (Carrier Free) $690/0.1 mg (Biotin) $690/0.1 mg (Cy3) $690/0.1 mg (Dylight488) $690/0.1 mg (Dylight550) $690/0.1 mg (Dylight594) $690/0.1 mg (FITC) $690/0.1 mg (HRP) $785/0.1 mg (APC) $785/0.1 mg (iFluor647) $785/0.1 mg (PE) $1,520/2x0.1 mg (APC) $1,940/5x0.1 mg $2,210/3x0.1 mg (APC) $2,865/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (DAB staining on IHC-P;Samples: Mouse Testis Tissue.)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Liver Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Kidney Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Brain Tissue.)

WB (Western Blot) (Western Blot:Sample:Lane 1: Rat Heart Tissue;Lane 2: Rat Testis Tissue.)

WB (Western Blot) (Western Blot:Sample: Recombinant BNP, Mouse.)

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Brain Natriuretic Peptide (BNP), Monoclonal Antibody (Cat# AAA21050)

Full Name

Monoclonal Antibody to Brain Natriuretic Peptide (BNP)

Gene Names

Nppb; BNF; BNP; AA408272

Reactivity

Mus musculus (Mouse)

Applications

Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).

Purity

Protein A + Protein G affinity chromatography

Pricing

$225/0.1 mL $275/0.2 mL $440/0.5 mL $530/1 mL $1,415/5 mL $2,745/2x5 mL $4,005/3x5 mL $5,195/4x5 mL $6,340/5x5 mL

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WB (Western Blot) (Sample: Human Lung lysate;Primary Ab: 2ug/ml Mouse Anti-Human MUC5AC AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))

WB (Western Blot) (Western Blot: Recombinant MUC5AC, Human.)

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Mucin 5 Subtype AC (MUC5AC), Monoclonal Antibody (Cat# AAA20289)

Full Name

Monoclonal Antibody to Mucin 5 Subtype AC (MUC5AC)

Gene Names

MUC5AC; TBM; leB; MUC5; mucin

Reactivity

Homo sapiens (Human)

Applications

Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).

Purity

Protein A + Protein G affinity chromatography

Pricing

$220/0.1 mL $270/0.2 mL $425/0.5 mL $510/1 mL $1,355/5 mL $2,630/2x5 mL $3,835/3x5 mL $4,975/4x5 mL $6,065/5x5 mL

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IHC (Immunohistochemistry) (Vector Red staining on IHC-PSamples:Human Small Intestine TissuePrimary Ab: 15ug/ml Mouse Anti-Human REG3a AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG)

WB (Western Blot) (Western Blot:Sample: Recombinant REG3a, Human)

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Regenerating Islet Derived Protein 3 Alpha (REG3a), Monoclonal Antibody (Cat# AAA20290)

Full Name

Monoclonal Antibody to Regenerating Islet Derived Protein 3 Alpha (REG3a)

Gene Names

REG3A; HIP; PAP; PAP1; REG3; INGAP; PAP-H; PBCGF; HIP/PAP; REG-III

Applications

Western Blot (WB); Immunohistochemistry (IHC)

Purity

Protein A + Protein G affinity chromatography

Pricing

$225/0.1 mL $285/0.2 mL $455/0.5 mL $560/1 mL $1,485/5 mL $2,885/2x5 mL $4,205/3x5 mL $5,455/4x5 mL $6,650/5x5 mL

WB (Western Blot) (Figure. Western Blot; Sample: Recombinant IFNg, Rabbit.)

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Interferon Gamma (IFNg), Monoclonal Antibody (Cat# AAA20291)

Full Name

Monoclonal Antibody to Interferon Gamma (IFNg)

Reactivity

Oryctolagus cuniculus (Rabbit)

Applications

Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).

Purity

Protein A + Protein G affinity chromatography

Pricing

$225/0.1 mL $280/0.2 mL $445/0.5 mL $540/1 mL $1,450/5 mL $2,815/2x5 mL $4,105/3x5 mL $5,325/4x5 mL $6,510/5x5 mL

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Application Data (S100A8 in NIH-3T3 mouse cell line: S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10ug/mL AAA14670 for 3 hours at RT. Cells were stained (red) and counterstained with DAPI (blue).)

WB (Western Blot) (Western Blot analysis of mouse S100A8 of lysates of mouse leukocyte. PVDF membrane was probed with AAA14670 (2ug/ml) followed by IgG (HRP) anti-rat IgG secondary antibody. A specific band was detected for S100A8 at ~10kD (as indicated). This experiment was conducted under reducing conditions and using the buffers listed in the protocol.)

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S100A8, Monoclonal Antibody (Cat# AAA14670)

Full Name

S100A8 (MRP-8)

Gene Names

S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; S100A8

Reactivity

Mouse

Applications

ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)

Purity

Purified by Protein G affinity chromatography from hybridoma culture supernatant.

Pricing

$1,040/0.1 mg $2,015/2x0.1 mg $2,940/3x0.1 mg $3,810/4x0.1 mg $4,450/5x0.1 mg

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IHC (Immunohistchemistry) (DAB staining on IHC-P Samples:Human Prostate Gland Tissue))

IHC (Immunohistochemistry) (DAB staining on IHC-P Samples:Human Glioma Tissue))

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples:Human Kidney Tissue))

WB (Western Blot) (Western Blot; Sample: Mouse Sp2/0 cell lysate; Primary Ab: 3ug/ml Mouse Anti-Human DKK1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot: Sample: Recombinant DKK1, Human.)

WB (Western Blot) (Western Blot: Sample: Rat Placenta Tissue.)

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Dickkopf Related Protein 1, Monoclonal Antibody (Cat# AAA20822)

Full Name

Dickkopf Related Protein 1 (DKK1)

Gene Names

DKK1; SK; DKK-1

Reactivity

Homo sapiens (Human)

Applications

Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).

Purity

Purification: Protein A + Protein G affinity chromatography

Pricing

$220/0.1 mL $270/0.2 mL $425/0.5 mL $510/1 mL $1,355/5 mL $2,630/2x5 mL $3,835/3x5 mL $4,975/4x5 mL $6,065/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Rat Lung Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))

IHC (Immunohistchemistry) (DAB staining on IHC-P;Samples: Rat Pancreas Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Rat Small Intestine Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Rat Stomach Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Rat Liver Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Rat Spleen Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))

WB (Western Blot) (Western Blot;Sample: Recombinant IL6, Rat.)

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Interleukin 6, Monoclonal Antibody (Cat# AAA20825)

Full Name

Monoclonal Antibody to Interleukin 6 (IL6)

Gene Names

Il6; ILg6; Ifnb2

Reactivity

Rattus norvegicus (Rat)

Applications

Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).

Purity

Protein A + Protein G affinity chromatography

Pricing

$225/0.1 mL $280/0.2 mL $445/0.5 mL $540/1 mL $1,450/5 mL $2,815/2x5 mL $4,105/3x5 mL $5,325/4x5 mL $6,510/5x5 mL

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IHC (Immunohistochemistry) (DAB staining on IHC-P Samples: Human Skin cancer TissuePrimary Ab: 30 ug/ml Mouse Anti-Human ADAM17 AntibodySecond Ab: 2 ug/mL HRP-Linked CaprineAnti-Mouse IgG PolyclonalAntibody (Catalog:))

WB (Western Blot) (Western Blot:Sample: Human U937 Cells.)

WB (Western Blot) (Western Blot:Sample: Recombinant ADAM17, Human.)

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A Disintegrin And Metalloprotease 17, Monoclonal Antibody (Cat# AAA20829)

Full Name

Monoclonal Antibody to A Disintegrin And Metalloprotease 17 (ADAM17)

Gene Names

ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1

Reactivity

Homo sapiens (Human)

Applications

Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).

Purity

Protein A + Protein G affinity chromatography

Pricing

$210/0.1 mL $240/0.2 mL $370/0.5 mL $450/1 mL $1,190/5 mL $2,310/2x5 mL $3,365/3x5 mL $4,365/4x5 mL $5,300/5x5 mL

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IHC (Immunohistchemistry) (DAB staining on IHC-P;Sample: Human Kidney Tissue;Primary Ab: 20ug/ml Mouse Anti-Human PIIINP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG PolyclonalAntibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Human Colon Tissue;Primary Ab: 20ug/ml Mouse Anti-Human PIIINP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Human Lung Tissue;Primary Ab: 20ug/ml Mouse Anti-Human PIIINP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IHC (Immunohistochemistry) (DAB staining on IHC-P;Sample: Human Placenta Tissue;Primary Ab: 20ug/ml Mouse Anti-Human PIIINP AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

IF (Immunofluorescence) (FITC staining on IF;Samples: Human HepG2 cell;Primary Ab: 20ug/ml Mouse Anti-Human PIIINP AntibodySecond Ab: 1ug/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

WB (Western Blot) (Sample: Human Hela cell lysate;Primary Ab: 2ug/ml Mouse Anti-Human PIIINP AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

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Procollagen III N-Terminal Propeptide, Monoclonal Antibody (Cat# AAA20833)

Full Name

Monoclonal Antibody to Procollagen III N-Terminal Propeptide (PIIINP)

Gene Names

COL3A1; EDS4A

Reactivity

Homo sapiens (Human)

Applications

Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).

Purity

Protein A + Protein G affinity chromatography

Pricing

$290/0.1 mL $365/0.2 mL $650/0.5 mL $790/1 mL $2,190/5 mL $4,260/2x5 mL $6,215/3x5 mL $8,065/4x5 mL $9,845/5x5 mL

WB (Western Blot) (Western Blot:Sample: Recombinant GnRH, Rat.)

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Gonadotropin Releasing Hormone (GnRH), Monoclonal Antibody (Cat# AAA20070)

Full Name

Monoclonal Antibody to Gonadotropin Releasing Hormone (GnRH)

Gene Names

Gnrh1; Gnrh; Lhrh; SH-4; Gnrha; Rgnrhg1

Reactivity

Rattus norvegicus (Rat)

Applications

Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).

Purity

Protein A + Protein G affinity chromatography

Pricing

$225/0.1 mL $280/0.2 mL $445/0.5 mL $540/1 mL $1,450/5 mL $2,815/2x5 mL $4,105/3x5 mL $5,325/4x5 mL $6,510/5x5 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.