Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged TAF11 is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (TAF11 monoclonal antibody (M04), clone 2G9 Western Blot analysis of TAF11 expression in Hela S3 NE (Cat # L013V3).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TAF11 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TAF11 on HeLa cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

Compare (5 max)

TAF11, Monoclonal Antibody (Cat# AAA26351)

Full Name

TAF11 (TAF11 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 28kD, MGC:15243, PRO2134, TAF2I, TAFII28) (FITC)

Gene Names

TAF11; TAF2I; PRO2134; TAFII28; MGC:15243

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Detection of human 53BP1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 second.)

IP (Immunoprecipitation) (Detection of human 53BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789 lot 1) used for IP at 20 ul per reaction. 53BP1 was also immunoprecipitated by rabbit anti-53BP1 antibody . For blotting immunoprecipitated 53BP1, AAA23789 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 second.)

IHC (Immunohistochemistry) (Detection of mouse 53BP1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of human 53BP1 by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry) (Detection of mouse 53BP1 (shaded) in mIMCD-3 cells by flow cytometry. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human 53BP1 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Compare (5 max)

53BP1, Monoclonal Recombinant Antibody (Cat# AAA23789)

Full Name

Rabbit anti-53BP1 Recombinant Monoclonal Antibody [BL-250-1H11]

Gene Names

TP53BP1; p202; 53BP1

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

Purified

Pricing

$265/0.02 mL $450/0.1 mL $865/2x0.1 mL $930/5x0.02 mL $1,255/3x0.1 mL $1,625/4x0.1 mL $1,765/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (CLK3 monoclonal antibody. Western Blot analysis of CLK3 expression in Hela NE.)

WB (Western Blot) (Western blot analysis of CLK3 over-expressed 293 cell line, cotransfected with CLK3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CLK3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CLK3 on HeLa cell. [antibody concentration 8ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CLK3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5ug/ml].)

WB (Western Blot) (Western Blot analysis of CLK3 expression in transfected 293T cell line by CLK3 monoclonal antibody.Lane 1: CLK3 transfected lysate (58.6kD).Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

Compare (5 max)

CLK3, Monoclonal Antibody (Cat# AAA24045)

Full Name

CLK3 (Dual Specificity Protein Kinase CLK3, CDC-like Kinase 3)

Reactivity

Human

Applications

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$770/0.1 mg $1,490/2x0.1 mg $2,170/3x0.1 mg $2,810/4x0.1 mg $3,235/5x0.1 mg

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged PAK1 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of PAK1 transfected lysate using anti-PAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PAK1 monoclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PAK1 on HeLa cell. [antibody concentration 35ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PAK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody. Lane 1: PAK1 transfected lysate (Predicted MW: 49.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PAK1 monoclonal antibody Western Blot analysis of PAK1 expression in HeLa NE.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

Compare (5 max)

PAK1, Monoclonal Antibody (Cat# AAA24301)

Full Name

PAK1 (Serine/Threonine-protein Kinase PAK 1, Alpha-PAK, p21-activated Kinase 1, PAK-1, p65-PAK, MGC130000, MGC130001) (AP)

Gene Names

PAK1; IDDMSSD; p65-PAK; PAKalpha; alpha-PAK

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (UBQLN2 monoclonal antibody. Western Blot analysis of UBQLN2 expression in NIH/3T3.)

WB (Western Blot) (UBQLN2 monoclonal antibody, Western Blot analysis of UBQLN2 expression in A-431.)

Application Data (Detection limit for recombinant GST tagged UBQLN2 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBQLN2 on A-431 cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (UBQLN2 monoclonal antibody. Western Blot analysis of UBQLN2 expression in Raw 264.7.)

WB (Western Blot) (UBQLN2 monoclonal antibody. Western Blot analysis of UBQLN2 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (33.81kD).)

Compare (5 max)

Ubiquilin-2, Monoclonal Antibody (Cat# AAA25581)

Full Name

Ubiquilin-2 (UBQLN2, Chap1, DSK2 Homolog, HRIHFB2157, N4BP4, Protein Linking IAP with Cytoskeleton 2, PLIC2, PLIC-2, hPLIC-2, Ubiquitin-like Product Chap1/Dsk2) (HRP)

Gene Names

UBQLN2; DSK2; ALS15; CHAP1; N4BP4; PLIC2; HRIHFB2157

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

IF (Immunofluorescence) (Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

IF (Immunofluorescence) (Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

IF (Immunofluorescence) (Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

WB (Western Blot) (Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

Compare (5 max)

Ubiquitin, Monoclonal Antibody (Cat# AAA28909)

Full Name

Ubiquitin mouse Monoclonal Antibody (5F1)

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Detection of mouse BRD4 by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)

WB (Western Blot) (Detection of human BRD4 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, MDA-MB-231, Hep-G2, A-549, SW620, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH .)

IP (Immunoprecipitation) (Detection of human BRD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 3) used for IP at 20 ul/mg lysate. BRD4 was also immunoprecipitated by a previous lot of this antibody (lot AAA23787-2). For blotting immunoprecipitated BRD4, AAA23787 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry) (Detection of human BRD4 by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 1) used at 1:50. Secondary: DyLight 594-conjugated goat anti-rabbit IgG antibody .)

IHC (Immunohistochemistry) (Detection of human BRD4 in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-151-6F11] (AAA23787 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human BRD4 by immunocytochemistry. Sample: formaldehyde-fixed Hep-G2 cells. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 1) used at 1:50. Secondary: DyLight 594-conjugated goat anti-rabbit IgG antibody .)

FCM (Flow Cytometry) (Detection of human BRD4 (shaded) in KG-1 cells by flow cytometry. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-151-6F11] (AAA23787) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Compare (5 max)

BRD4, Monoclonal Recombinant Antibody (Cat# AAA23787)

Full Name

Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-151-6F11]

Gene Names

BRD4; CAP; MCAP; HUNK1; HUNKI

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry-Immunofluorescence (IHC-IF), Immunocytochemistry-Immunofluorescence (ICC-IF), Flow Cytometry (FC/FACS)

Purity

Purified

Pricing

$265/0.02 mL $450/0.1 mL $865/2x0.1 mL $930/5x0.02 mL $1,255/3x0.1 mL $1,625/4x0.1 mL $1,765/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (ASB10 monoclonal antibody. Western Blot analysis of ASB10 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged ASB10 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (ASB10 monoclonal antibody, Western Blot analysis of ASB10 expression in A-431.)

WB (Western Blot) (ASB10 monoclonal antibody. Western Blot analysis of ASB10 expression in Raw 264.7.)

WB (Western Blot) (ASB10 monoclonal antibody. Western Blot analysis of ASB10 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.77kD).)

Compare (5 max)

ASB10, Monoclonal Antibody (Cat# AAA24043)

Full Name

ASB10 (Ankyrin Repeat and SOCS Box Protein 10, ASB-10)

Gene Names

ASB10; GLC1F

Reactivity

Human, Mouse, Rat

Applications

ELISA (EL/EIA), Western Blot (WB)

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$770/0.1 mg $1,490/2x0.1 mg $2,170/3x0.1 mg $2,810/4x0.1 mg $3,235/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (LIMK1 monoclonal antibody. Western Blot analysis of LIMK1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

Application Data (Detection limit for recombinant GST tagged LIMK1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LIMK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])

WB (Western Blot) (LIMK1 monoclonal antibody. Western Blot analysis of LIMK1 expression in NIH/3T3.)

WB (Western Blot) (LIMK1 monoclonal antibody. Western Blot analysis of LIMK1 expression in Raw 264.7.)

WB (Western Blot) (LIMK1 monoclonal antibody Western Blot analysis of LIMK1 expression in SW-13.)

Compare (5 max)

LIMK1, Monoclonal Antibody (Cat# AAA24555)

Full Name

LIMK1 (LIM Domain Kinase 1, LIMK-1, LIMK) APC

Gene Names

LIMK1; LIMK; LIMK-1

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of SGK over-expressed 293 cell line, cotransfected with SGK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SGK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged SGK is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SGK on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human stomach tissue. [antibody concentration 5ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of SGK expression in transfected 293T cell line by SGK monoclonal antibody. Lane 1: SGK transfected lysate (48.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (73.52kD).)

Compare (5 max)

SGK, Monoclonal Antibody (Cat# AAA25835)

Full Name

SGK (SGK1, Serine/Threonine-protein Kinase SGK1, Serum/Glucocorticoid-regulated Kinase 1) (PE)

Gene Names

SGK1; SGK

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7. Western Blot analysis of RIPK2 expression in PC-12 (Cat # L012V1).)

WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7. Western Blot analysis of RIPK2 expression in NIH/3T3 (Cat # L018V1).)

Application Data (Detection limit for recombinant GST tagged RIPK2 is approximately 0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.2 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.2 ug/ml])

WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7 Western Blot analysis of RIPK2 expression in HeLa (Cat # L013V1).)

Compare (5 max)

RIPK2, Monoclonal Antibody (Cat# AAA26603)

Full Name

RIPK2 (Receptor-Interacting Serine-Threonine Kinase 2, CARD3, CARDIAK, CCK, GIG30, RICK, RIP2) (PE)

Gene Names

RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200)

Compare (5 max)

Bax, Monoclonal Antibody (Cat# AAA28907)

Full Name

Bax mouse Monoclonal Antibody (6F11)

Gene Names

BAX; BCL2L4

Reactivity

Human, Mouse, Rat, Chicken

Applications

Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)

Pricing

$480/0.1 mL $925/2x0.1 mL $1,345/3x0.1 mL $1,740/4x0.1 mL $1,975/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of PD-L1 in human tonsil tissue with PD-L1 antibody at 5 μg/mL.)

IHC (Immunohistchemistry) (Immunohistochemistry of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 5 μg/mL.)

IF (Immunofluorescence) (Immunofluorescence of PD-L1 in human tonsil tissue with PD-L1 antibody at 2 μg/mL.Red: PDL1 Antibody [2D6]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of PD-L1 in human stomach carcinoma tissue with PD-L1 antibody at 2 μg/mL.Red: PDL1 Antibody [2D6]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 2 μg/mL.Red: PDL1 Antibody [2D6]Blue: DAPI staining)

ICC (Immunocytochemistry) (Immunocytochemistry of PD-L1 in transfected HEK293 cells with PD-L1 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)

WB (Western Blot) (Western blot analysis of PD-L1 in overexpressing HEK293 cells PD-L1 antibody at 0.25 and 0.5 μg/ml)

Compare (5 max)

PDL1, Monoclonal Antibody (Cat# AAA10993)

Full Name

PDL1 Antibody [2D6]

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Reactivity

Human

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF)

Purity

Protein A purified

Pricing

$530/0.1 mg $1,020/2x0.1 mg $1,485/3x0.1 mg $1,925/4x0.1 mg $2,195/5x0.1 mg

< >

<< Previous Next >>

Application Data (Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. Low power)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD8beta antibody, clone 341 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD8beta antibody, clone 341 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)

Application Data (Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. High power)

Application Data (Rat splenocytes stained with Mouse anti Rat CD8 Beta:FITC)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD8beta antibody, clone 341 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)

Application Data (Immunofluorescesence staining of rat lymph node cryosection with Mouse anti Rat CD8beta, clone 341 , red in A and Mouse anti Rat anti Mouse CD4 antibody, clone W3/25 , green in B. C is the merged picture C with nuclei counterstained blue using DAPI. Medium power)

Compare (5 max)

CD8 BETA, Monoclonal Antibody (Cat# AAA12017)

Full Name

MOUSE ANTI RAT CD8 BETA

Gene Names

Cd8b; Cd8b1

Applications

Flow cytometry (FC/FACS), Immunoprecipitation (IP)

Pricing

$430/0.1 mg $825/2x0.1 mg $1,200/3x0.1 mg $1,555/4x0.1 mg $1,680/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Detection of human CD3E by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, K-562, SK-MEL-28, MOLT-4, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (AAA23793 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-GAPDH .)

IP (Immunoprecipitation) (Detection of human CD3E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (AAA23793 lot 2) used for IP at 20 ul/mg lysate. CD3E was also immunoprecipitated by a previous lot of this antibody (lot AAA23793-1). For blotting immunoprecipitated CD3E, AAA23793 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry) (Detection of human CD3E by immunhistochemistry. Sample: FFPE section of appendix. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (AAA23793 lot 1) used at 1:250. Secondary: DyLight 594-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry) (Detection of human CD3E (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (AAA23793 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)

IHC (Immunohistochemistry) (Detection of human CD3E in FFPE tonsil by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (AAA23793 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of human CD3E in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] (AAA23793 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry) (Detection of human CD3E by flow cytometry. Sample: Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 1 ul of rabbit anti-CD3E recombinant monoclonal antibody [BL-298-5D12] (AAA23793 lot 1) (red shaded) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Compare (5 max)

CD3E, Monoclonal Recombinant Antibody (Cat# AAA23793)

Full Name

Rabbit anti-CD3E Recombinant Monoclonal Antibody [BL-298-5D12]

Gene Names

CD3E; T3E; TCRE; IMD18

Reactivity

Human

Applications

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS), Multiplex immunofluorescence (mIF)

Purity

Purified

Pricing

$265/0.01 mL $640/0.1 mL $930/5x0.01 mL $1,235/2x0.1 mL $1,800/3x0.1 mL $2,330/4x0.1 mL $2,635/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (ACBD3 monoclonal antibody. Western Blot analysis of ACBD3 expression in PC-12.)

WB (Western Blot) (ACBD3 monoclonal antibody, Western Blot analysis of ACBD3 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged ACBD3 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACBD3 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACBD3 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of ACBD3 expression in transfected 293T cell line by ACBD3 monoclonal antibody.Lane 1: ACBD3 transfected lysate (60.6kD).Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

Compare (5 max)

ACBD3, Monoclonal Antibody (Cat# AAA24049)

Full Name

ACBD3 (Golgi Resident Protein GCP60, Acyl-CoA-binding Domain-containing Protein 3, Golgi Complex-associated Protein 1, GOCAP1, Golgi Phosphoprotein 1, GOLPH1, PBR- and PKA-associated Protein 7, Peripheral Benzodiazepine Receptor-associated Protein PAP7, G

Gene Names

ACBD3; PAP7; GCP60; GOCAP1; GOLPH1

Reactivity

Human, Mouse, Rat

Applications

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$770/0.1 mg $1,490/2x0.1 mg $2,170/3x0.1 mg $2,810/4x0.1 mg $3,235/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (DARS monoclonal antibody Western Blot analysis of DARS expression in HeLa.)

WB (Western Blot) (Western blot analysis of DARS over-expressed 293 cell line, cotransfected with DARS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DARS monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged DARS is ~0.3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DARS on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of DARS expression in transfected 293T cell line by DARS monoclonal antibody. Lane 1: DARS transfected lysate (57.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.62kD).)

Compare (5 max)

DARS, Monoclonal Antibody (Cat# AAA25073)

Full Name

DARS (Aspartyl-tRNA Synthetase, Cytoplasmic, Aspartate-tRNA Ligase, AspRS, Cell Proliferation-inducing Gene 40 Protein, PIG40) (FITC)

Gene Names

DARS; HBSL; aspRS

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

IP (Immunoprecipitation) (Immunoprecipitation of USP33 transfected lysate using anti-USP33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with USP33 rabbit polyclonal antibody.)

Application Data (Detection limit for recombinant GST tagged USP33 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USP33 on HeLa cell. [antibody concentration 20ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to USP33 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of USP33 expression in transfected 293T cell line by USP33 monoclonal antibody Lane 1: USP33 transfected lysate (103.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

Compare (5 max)

USP33, Monoclonal Antibody (Cat# AAA25585)

Full Name

USP33 (Ubiquitin-specific-processing Protease 33, Ubiquitin Carboxyl-terminal Hydrolase 33, Deubiquitinating Enzyme 33, Ubiquitin Thioesterase 33, VHL-interacting Deubiquitinating Enzyme 1, VDU1, hVDU1, KIAA1097) (HRP)

Gene Names

USP33; VDU1

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (SMAD4 monoclonal antibody. Western Blot analysis of SMAD4 expression in IMR-32.)

WB (Western Blot) (Western Blot detection against Immunogen (86.46kD).)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CDKN1A and SMAD4 HeLa cells were stained with CDKN1A rabbit purified polyclonal 1:1200 and SMAD4 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged SMAD4 is ~3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD4 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (SMAD4 monoclonal antibody Western Blot analysis of SMAD4 expression in Hela NE.)

Compare (5 max)

SMAD4, Monoclonal Antibody (Cat# AAA25841)

Full Name

SMAD4 (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP Homolog 4, Deletion Target In Pancreatic Carcinoma 4, MAD Homolog 4, SMAD Family Member 4, hSMAD4, DPC4, MADH4) (PE)

Gene Names

SMAD4; JIP; DPC4; MADH4; MYHRS

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining Cyclin A2 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Cyclin A2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Cyclin A2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cyclin A2 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Cyclin A2 on different lysates using anti-Cyclin A2 antibody at 1/1, 000 dilution. Positive control: Lane 1: PC12 Lane 2: NIH/3T3)

Compare (5 max)

Cyclin A2, Monoclonal Antibody (Cat# AAA30193)

Full Name

Cyclin A2 Antibody

Gene Names

CCNA2; CCN1; CCNA

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (AAA31473 at 1/100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA31473 at 1/100 staining mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA31473 at 1/100 staining mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA31473 at 1/100 staining human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA31473 at 1/100 staining human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from various samples, using Nucleophosmin Mouse Monoclonal Antibody. Lane 1: Hela cells treated with blocking peptide; Lane 2: Hela cells; Lane 3: B16F10-LUC cells. Lane 4: Rat lung tissue.)

Compare (5 max)

Nucleophosmin, Monoclonal Antibody (Cat# AAA31473)

Full Name

Nucleophosmin Mouse Monoclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC)

Purity

Affinity-chromatography.

Pricing

$360/0.1 mL $405/0.2 mL $780/2x0.2 mL $1,130/3x0.2 mL $1,460/4x0.2 mL $1,580/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)

Application Data (Detection limit for recombinant GST tagged MAGEA12 is 0.03ng/ml as a capture antibody.)

WB (Western Blot) (MAGEA12 monoclonal antibody. Western Blot analysis of MAGEA12 expression in NIH/3T3.)

WB (Western Blot) (MAGEA12 monoclonal antibody. Western Blot analysis of MAGEA12 expression in Jurkat.)

WB (Western Blot) (MAGEA12 monoclonal antibody. Western Blot analysis of MAGEA12 expression in Raw 264.7.)

WB (Western Blot) (MAGEA12 monoclonal antibody. Western Blot analysis of MAGEA12 expression in human liver.)

Compare (5 max)

MAGEA12, Monoclonal Antibody (Cat# AAA24558)

Full Name

MAGEA12 (Melanoma-associated Antigen 12, MAGE-12 Antigen, MAGE-12F Antigen, Cancer/Testis Antigen 1.12, CT1.12, MAGE12) APC

Gene Names

MAGEA12; CT1.12; MAGE12

Reactivity

Human, Mouse

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

WB (Western Blot) (Western blot analysis of ASNA1 over-expressed 293 cell line, cotransfected with ASNA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ASNA1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged ASNA1 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ASNA1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ASNA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.5ug/ml])

WB (Western Blot) (Western Blot analysis of ASNA1 expression in transfected 293T cell line by ASNA1 monoclonal antibody. Lane 1: ASNA1 transfected lysate (38.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ASNA1 monoclonal antibody. Western Blot analysis of ASNA1 expression in PC-12.)

Compare (5 max)

ASNA1, Monoclonal Antibody (Cat# AAA25326)

Full Name

ASNA1 (ATPase ASNA1, Arsenical Pump-driving ATPase, Arsenite-stimulated ATPase, Transmembrane Domain Recognition Complex 40kD ATPase Subunit, hARSA-I, hASNA-I, ARSA, TRC40) (HRP)

Gene Names

ASNA1; GET3; ARSA1; TRC40; ARSA-I; ASNA-I

Reactivity

Human, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1 HeLa cells were stained with anti-HSP90AB1 rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])

WB (Western Blot) (Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.Lane 1: NFKB1 transfected lysate(105.4 KDa).Lane 2: Non-transfected lysate.)

Compare (5 max)

NFKB1, Monoclonal Antibody (Cat# AAA26094)

Full Name

NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1, DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NFKB-p105, NFKB-p50, p105, p50) (APC)

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB), In situ Proximity Ligation Assay (Cell)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using GAPDH Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using GAPDH Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using GAPDH Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human esophageal using GAPDH Mouse mAb at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using GAPDH antibody at 1:10000-1:2560000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GAPDH Monoclonal Antibody at 1:20000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

Compare (5 max)

GAPDH, Monoclonal Antibody (Cat# AAA28398)

Full Name

GAPDH Mouse mAb (High Dilution)

Gene Names

GAPDH; G3PD; GAPD

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity purification

Pricing

$220/0.02 mL $300/0.1 mL $575/2x0.1 mL $830/3x0.1 mL $1,070/4x0.1 mL $1,085/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged GPR3 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GPR3 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody. Lane 1: GPR3 transfected lysate (35kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GPR3 monoclonal antibody, Western Blot analysis of GPR3 expression in Jurkat.)

WB (Western Blot) (Western Blot detection against Immunogen (62.04kD).)

Compare (5 max)

GPR3, Monoclonal Antibody (Cat# AAA24818)

Full Name

GPR3 (G-protein Coupled Receptor 3, ACCA Orphan Receptor, ACCA) (Biotin)

Gene Names

GPR3; ACCA

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in Hela NE.)

WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged BATF is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of BATF transfected lysate using BATF monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BATF rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of BATF expression in transfected 293T cell line by BATF monoclonal antibody Lane 1: BATF transfected lysate (14.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (36.23kD).)

Compare (5 max)

BATF, Monoclonal Antibody (Cat# AAA25330)

Full Name

BATF (Basic Leucine Zipper Transcriptional Factor ATF-like, B Cell-activating Transcription Factor, SF-HT-activated Gene 2 Protein) (HRP)

Gene Names

BATF; SFA2; B-ATF; BATF1; SFA-2

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).Lane 2: Non-transfected lysate.)

IP (Immunoprecipitation) (Immunoprecipitation of MPP1 transfected lysate using anti-MPP1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MPP1 monoclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MPP1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MPP1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (MPP1 monoclonal antibody (M01), clone 1E11-1G11. Western Blot analysis of MPP1 expression in human placenta.)

Application Data (Detection limit for recombinant GST tagged MPP1 is approximately 1ng/ml as a capture antibody.)

Compare (5 max)

MPP1, Monoclonal Antibody (Cat# AAA26610)

Full Name

MPP1 (Membrane Protein, Palmitoylated 1, 55kD, AAG12, DXS552, DXS552E, EMP55, MRG1, PEMP) (PE)

Gene Names

MPP1; MRG1; PEMP; AAG12; EMP55; DXS552E

Applications

Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining PDPK1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PDPK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PDPK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of PDPK1 on different lysates using anti-PDPK1 antibody at 1/1, 000 dilution. Positive control: Lane 1: 293T Lane 2: PC-12 Lane 3: HL-60)

Compare (5 max)

PDPK1, Monoclonal Antibody (Cat# AAA30194)

Full Name

PDPK1 Antibody

Gene Names

PDPK1; PDK1; PDPK2; PRO0461

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

IF (Immunofluorescence) (Immunofluorescence analysis of 293T cells by overexpressed SARS-CoV Spike Protein (Right) or not (Left) using SARS-CoV Spike Monoclonal Antibody at dilution of 1:50.)

Compare (5 max)

COVID 19 Spike Protein Coronavirus, Monoclonal Antibody (Cat# AAA22149)

Full Name

SARS-COV/SARS-COV-2 Spike Monoclonal Antibody(2019-nCoV)

Reactivity

SARS-COV2

Applications

ELISA (EIA), Immunofluorescence (IF)

Pricing

$345/0.05 mL $505/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,830/4x0.1 mL $2,205/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (HHIP monoclonal antibody. Western Blot analysis of HHIP expression in MCF-7.)

WB (Western Blot) (HHIP monoclonal antibody, Western Blot analysis of HHIP expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged HHIP is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of HHIP transfected lysate using HHIP monoclonal antibody and Protein A Magnetic Bead and immunoblotted with HHIP rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HHIP on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of HHIP expression in transfected 293T cell line by HHIP monoclonal antibody. Lane 1: HHIP transfected lysate (78.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

Compare (5 max)

HHIP, Monoclonal Antibody (Cat# AAA24827)

Full Name

HHIP (Hedgehog-interacting Protein, HHIP, HIP, UNQ5825/PRO19644, FLJ20992, FLJ90230) (Biotin)

Gene Names

HHIP; HIP

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (DYRK1A monoclonal antibody. Western Blot analysis of DYRK1A expression in NIH/3T3.)

WB (Western Blot) (DYRK1A monoclonal antibody. Western Blot analysis of DYRK1A expression in 293.)

Application Data (Detection limit for recombinant GST tagged DYRK1A is ~0.03ng/ml as a capture antibody.)

WB (Western Blot) (DYRK1A monoclonal antibody. Western Blot analysis of DYRK1A expression in Raw 264.7.)

WB (Western Blot) (DYRK1A monoclonal antibody, Western Blot analysis of DYRK1A expression in Hela NE.)

WB (Western Blot) (DYRK1A monoclonal antibody. Western Blot analysis of DYRK1A expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

Compare (5 max)

DYRK1A, Monoclonal Antibody (Cat# AAA25083)

Full Name

DYRK1A (Dual Specificity Tyrosine-phosphorylation-regulated Kinase 1A, Protein Kinase Minibrain Homolog, MNBH, HP86, Dual Specificity YAK1-related Kinase, hMNB, DYRK, MNB, MNBH) (FITC)

Gene Names

DYRK1A; MNB; DYRK; HP86; MNBH; MRD7; DYRK1

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (CAPNS1 monoclonal antibody, Western Blot analysis of CAPNS1 expression in A-431.)

WB (Western Blot) (Western blot analysis of CAPNS1 over-expressed 293 cell line, cotransfected with CAPNS1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAPNS1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged CAPNS1 is ~0.1ng/ml when antibody 124328 was used as capture antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of CAPNS1 on HeLa cells using antibody 124328 at 20ug/ml.)

IHC (Immunohistochemistry) (Immunoperoxidase staining of CAPNS1 on formalin-fixed paraffin-embedded human kidney using antibody 124328 at 3ug/ml.)

WB (Western Blot) (Western Blot analysis of CAPNS1 expression in transfected 293T cell line using antibody 124328: Lane 1: CAPNS1 transfected lysate (28.3kD) Lane 2: Non-transfected lysate)

WB (Western Blot) (Western Blot analysis of CAPNS1 expression in PC-12 cells using antibody 124328.)

Compare (5 max)

CAPNS1, Monoclonal Antibody (Cat# AAA25339)

Full Name

CAPNS1 (Calpain Small Subunit 1, CSS1, Calcium-activated Neutral Proteinase Small Subunit, CANP Small Subunit, Calcium-dependent Protease Small Subunit, CDPS, Calcium-dependent Protease Small Subunit 1, Calpain Regulatory Subunit, CAPN4, CAPNS) (HRP)

Gene Names

CAPNS1; CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4

Reactivity

Human, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged SP1 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1ug/ml].)

WB (Western Blot) (SP1 monoclonal antibody. Western Blot analysis of SP1 expression in NIH/3T3.)

WB (Western Blot) (SP1 monoclonal antibody, Western Blot analysis of SP1 expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

Compare (5 max)

SP1, Monoclonal Antibody (Cat# AAA25851)

Full Name

SP1 (Transcription Factor Sp1, TSFP1) (PE)

Reactivity

Human, Mouse

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (NME2 monoclonal antibody (M11), clone 1E4. Western Blot analysis of NME2 expression in PC-12.)

WB (Western Blot) (NME2 monoclonal antibody (M11), clone 1E4. Western Blot analysis of NME2 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged NME2 is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (NME2 monoclonal antibody (M11), clone 1E4 Western Blot analysis of NME2 expression in HeLa.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NME2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NME2 on HeLa cell. [antibody concentration 10 ug/ml])

Compare (5 max)

NME2, Monoclonal Antibody (Cat# AAA26107)

Full Name

NME2 (Non-Metastatic Cells 2, Protein (NM23B) Expressed in, MGC111212, NDPK-B, NDPKB, NM23-H2, NM23B, puf) (APC)

Gene Names

NME2; PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2

Applications

Immunofluorescence (IF), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with TRAF2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining TRAF2 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TRAF2 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TRAF2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TRAF2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-TRAF2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TRAF2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TRAF2 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of TRAF2 on Hela cells lysates using anti-TRAF2 antibody at 1/1, 000 dilution.)

Compare (5 max)

TRAF2, Monoclonal Antibody (Cat# AAA30203)

Full Name

TRAF2 Antibody

Gene Names

TRAF2; TRAP; TRAP3; MGC:45012

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

< >

<< Previous Next >>

ELISA (Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA at decreasing concentrations.)

FCM (Flow Cytometry) (Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.)

IHC (Immunohistochemistry) (Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 μg/ml.)

IF (Immunofluorescence) (Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 μg/ml.Green: TIGIT Antibody [4A10]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 μg/ml.Green: TIGIT Antibody [4A10]Blue: DAPI staining)

ICC (Immunocytochemistry) (Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.)

WB (Western Blot) (Western blot analysis of TIGIT in over expressing HEK293 cells using antibody at (A) 0.25 μg/ml , (B) 0.5 μg/ml, and (C) 1 μg/ml.)

Compare (5 max)

TIGIT, Monoclonal Antibody (Cat# AAA11004)

Full Name

TIGIT Antibody [4A10]

Gene Names

TIGIT; VSIG9; VSTM3; WUCAM

Reactivity

Human

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Protein A purified

Pricing

$530/0.1 mg $1,020/2x0.1 mg $1,485/3x0.1 mg $1,925/4x0.1 mg $2,195/5x0.1 mg

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with AF488 Conjugate of Nucleolin Monoclonal Antibody (364-5 + NCL/902).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Uterus stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolin Monoclonal Antibody (364-5 + NCL/902)

Compare (5 max)

Nucleolin, Monoclonal Antibody (Cat# AAA13820)

Full Name

Nucleolin (Marker of Human Cells) Mouse Monoclonal Antibody

Gene Names

NCL; C23

Reactivity

Human.
Does not react with Mouse, Rat and Cow

Applications

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

Pricing

$265/0.02 mg (With BSA & Azide at 0.2mg/mL) $445/0.1 mg (With BSA & Azide at 0.2mg/mL) $445/0.1 mg (Without BSA & Azide at 1mg/mL) $855/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,245/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,610/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,810/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,810/5x0.1 mg (Without BSA & Azide at 1mg/mL)

< >

<< Previous Next >>

ELISA (Titration curve analysis of TIGIT mAbs to detect recombinant TIGIT in ELISA at decreasing concentrations.)

FCM (Flow Cytometry) (Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.)

IHC (Immunohistochemistry) (Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 μg/ml.)

IF (Immunofluorescence) (Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 μg/ml.Green: TIGIT Antibody [4A12]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 μg/ml.Green: TIGIT Antibody [4A12]Blue: DAPI staining)

ICC (Immunocytochemistry) (Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.)

WB (Western Blot) (Western blot analysis of TIGIT in over expressing HEK293 cells using antibody at (A) 0.25 μg/ml , (B) 0.5 μg/ml, and (C) 1 μg/ml.)

Compare (5 max)

TIGIT, Monoclonal Antibody (Cat# AAA11006)

Full Name

TIGIT Antibody [4A12]

Gene Names

TIGIT; VSIG9; VSTM3; WUCAM

Reactivity

Human

Applications

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Purity

Protein A purified

Pricing

$530/0.1 mg $1,020/2x0.1 mg $1,485/3x0.1 mg $1,925/4x0.1 mg $2,195/5x0.1 mg

< >

<< Previous Next >>

Application Data (Figure A. FITC conjugated Rat anti Mouse CD19 . Figure B. FITC conjugated Rat anti Mouse CD19 and SBV440 conjugated Rat anti Mouse CD45R . All experiments performed on mouse splenocytes in the presence of 10% mouse seru)

Application Data (Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD45R . Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD45R and SBV515 conjugated Rat anti Mouse CD19 . All experiments performed on red cell lysed mouse b)

Application Data (Figure A. FITC conjugated Rat anti Mouse CD19 . Figure B. FITC conjugated Rat anti Mouse CD19 and SBV515 conjugated Rat anti Mouse CD45R . All experiments performed on red cell lysed mouse blood gated on live single cel)

Application Data (Figure A. FITC conjugated rat anti mouse CD16/CD32 and Alexa647 conjugated Rat IgG2a isotype control . Figure B. FITC conjugated rat anti mouse CD16/CD32 and Alexa647 conjugated rat anti mouse CD19 . All exp)

Application Data (Figure A. RPE conjugated rat anti mouse CD3 and Alexa647 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated rat anti mouse CD3 and Alexa647 conjugated rat anti mouse CD19 . All experiments perf)

Application Data (Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated Rat IgG2a isotype control . Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD19 . All experiments performed on)

IF (Immunofluorescence) (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti MouseCD19 antibody, clone 6D5 , green in A and Rat anti Mlouse CD8 antibody, clone YTS105.18 , red in B. Merged image in C with nuclei counterstained blue using)

IF (Immunofluorescence) (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD19 antibody, clone 6D5 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. Merged image in C with nuclei counterstained blue using)

Application Data (Immunoperoxidase staining of a murine lymph node cryosections with Rat anti Mouse CD19 antibody, clone 6D5 followed by horseradish peroxidase conjugated Goat anti Rat IgG. High power)

Application Data (Immunoperoxidase staining of a murine lymph node cryosections with Rat anti Mouse CD19 antibody, clone 6D5 followed by horseradish peroxidase conjugated Goat anti Rat IgG. Low power)

Compare (5 max)

CD19, Monoclonal Antibody (Cat# AAA12286)

Full Name

Rat anti Mouse CD19:Amethyst Orange

Gene Names

Cd19; AW495831

Reactivity

Mouse

Applications

Flow Cytometry (FC/FACS)

Purity

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Pricing

$585/0.1 mg $1,130/2x0.1 mg $1,640/3x0.1 mg $2,125/4x0.1 mg $2,380/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (EHD3 monoclonal antibody. Western Blot analysis of EHD3 expression in COLO 320 HSR.)

WB (Western Blot) (EHD3 monoclonal antibody, Western Blot analysis of EHD3 expression in IMR-32.)

Application Data (Detection limit for recombinant GST tagged EHD3 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EHD3 on HeLa cell. [antibody concentration 25ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EHD3 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)

WB (Western Blot) (EHD3 monoclonal antibody. Western Blot analysis of EHD3 expression in MCF-7.)

WB (Western Blot) (Western Blot detection against Immunogen (31.61kD).)

Compare (5 max)

EHD3, Monoclonal Antibody (Cat# AAA24062)

Full Name

EHD3 (EH Domain-containing Protein 3, PAST Homolog 3, EHD2, PAST3)

Gene Names

EHD3; PAST3

Reactivity

Human

Applications

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$770/0.1 mg $1,490/2x0.1 mg $2,170/3x0.1 mg $2,810/4x0.1 mg $3,235/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (EIF2S1 monoclonal antibody, Western Blot analysis of EIF2S1 expression in HeLa.)

WB (Western Blot) (EIF2S1 monoclonal antibody. Western Blot analysis of EIF2S1 expression in Hela NE.)

WB (Western Blot) (Western blot analysis of EIF2S1 over-expressed 293 cell line, cotransfected with EIF2S1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2S1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged EIF2S1 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EIF2S1 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of EIF2S1 expression in transfected 293T cell line by EIF2S1 monoclonal antibody. Lane 1: EIF2S1 transfected lysate (36.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (60.39kD).)

Compare (5 max)

EIF2S1, Monoclonal Antibody (Cat# AAA25086)

Full Name

EIF2S1 (Eukaryotic Translation Initiation Factor 2, Subunit 1 alpha, eIF2 alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2A, Eukaryotic Translation Initiation Factor 2 Subunit 1) (FITC)

Gene Names

EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western Blot detection against Immunogen (37.44kD).)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in human pancreas.)

Application Data (Detection limit for recombinant GST tagged CCT7 is ~0.3ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of CCT7 expression in transfected 293T cell line by CCT7 monoclonal antibody. Lane 1: CCT7 transfected lysate (59.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in K-562.)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in Raw 264.7)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in HL-60)

Compare (5 max)

CCT7, Monoclonal Antibody (Cat# AAA25342)

Full Name

CCT7 (T-complex Protein 1 Subunit eta, TCP-1-eta, CCT-eta, HIV-1 Nef-interacting Protein, CCTH, NIP7-1) (HRP)

Gene Names

CCT7; CCTH; CCTETA; NIP7-1; TCP1ETA

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in HeLa NE.)

WB (Western Blot) (ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged ACTN4 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACTN4 on HeLa cell. [antibody concentration 20ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTN4 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody. Lane 1: ACTN4 transfected lysate (104.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in PC-12.)

Compare (5 max)

ACTN4, Monoclonal Antibody (Cat# AAA25598)

Full Name

ACTN4 (Alpha-actinin-4, Non-muscle alpha-actinin 4, F-actin Cross-linking Protein) (PE)

Gene Names

ACTN4; FSGS; FSGS1; ACTININ-4

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

< >

<< Previous Next >>

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 488)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Low Endotoxin)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . Medium power)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD204:FITC)

Application Data (Western Blot staining of J774 lysate (non reduced) probed with Rat anti Mouse CD204)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Biotin)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . High power)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 647)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204:FITC)

Compare (5 max)

CD204, Monoclonal Antibody (Cat# AAA12075)

Full Name

RAT ANTI MOUSE CD204:Low Endotoxin

Gene Names

Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1

Applications

Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS), Functional Assays (FN), Immunoprecipitation (IP), Western Blot (WB)*

Pricing

$865/0.5 mg $1,675/2x0.5 mg $2,440/3x0.5 mg $3,160/4x0.5 mg $3,630/5x0.5 mg

< >

<< Previous Next >>

Application Data (Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 488 (AAA11941A488))

Application Data (Published customer image: Blockade of the mevalonate pathway increases CD9 and CD81. (A) RAW264.7 cells were untreated (-) or treated for 48 h with 50 ng/ml TSA (+) in the absence (-) or presence of 50 uM theophylline or 0.5 uM fluvastatin (Fluv) (+). The cells were lysed, and levels of CD9 and CD81 were examined by immunoblotting. Anti-actin blots show that comparable amounts of protein were loaded in each lane. (B) The mevalonate pathway and inhibitors. n-BP, nitrogenous bisphosphonate. (C) RAW264.7 cells were cultured for 24 h in the presence of indicated concentrations of fluvastatin, simvastatin (Simv), zoledronate (Zol), or risedronate (Ris). Levels of CD9 and CD81 were examined by immunoblotting. (D) RAW264.7 cells were cultured for 24 h in the absence (V, vehicle alone) or presence of mevalonate (Mev), farnesyl pyrophosphate (FPP), squalene (Squ), or geranylgeranyl pyrophosphate (GGPP). Although the actin level in the GGPP lane appears to be lower, an equal amount of protein was loaded. (E) RAW264.7 cells were cultured for 24 h in the absence (V) or presence of fluvastatin, zoledronate, farnesyl transferase inhibitor (FTI), or geranylgeranyl transferase inhibitor (GGTI). (F) RAW264.7 cells were untreated (-) or treated with fluvastatin (+) in the absence (V) or presence of mevalonate, FPP, squalene, or GGPP. (G) RAW264.7 cells were untreated (-) or treated with zoledronate (+) in the absence (V) or presence of mevalonate, FPP, squalene, or GGPP. (H) RAW264.7 cells were untreated (-) or treated with fluvastatin (+) in the absence (V) or presence of mevalonate, FPP, squalene, or GGPP and stimulated for 15 min with 0.1 ug/ml LPS (+). The cells were lysed, and levels of I?Ba were examined by immunoblotting. (I) RAW264.7 cells were cultured for 24 h in the indicated concentrations of HA1077. Levels of CD9 and CD81 were examined by immunoblotting.From: Jin Y, Tachibana I, Takeda Y, He P, Kang S, et al. (2013) Statins Decrease Lung Inflammation in Mice by Upregulating Tetraspanin CD9 in Macrophages. PLoS ONE 8(9): e73706.)

Application Data (Published customer image: Screening of a drug library for agents that upregulate CD9 or CD81 in RAW264.7 macrophages. (A) RAW264.7 cells were cultured for 24 h in the absence (V, vehicle alone) and presence of each drug (10 uM). The cells were lysed, and levels of CD9 and CD81 were examined by immunoblotting. Blots of results with fluvastatin (Fluv) and simvastatin (Simv) are shown. Anti-actin blots show that comparable amounts of protein were loaded in each lane. (B) After testing 1,165 drugs, levels of CD9 and CD81 relative to actin were quantified by densitometry. Fold changes of the expression levels compared with vehicle alone were calculated and plotted. Drugs that increased the level of either CD9 or CD81 more than 1.5-fold compared with vehicle alone were regarded as positive. Correlation between fold changes in CD9 and CD81 levels was analyzed using Pearson's correlation coefficient. (C) RAW264.7 cells were cultured in the absence (V) or presence of multiple statins (10 uM) and levels of CD9 and CD81 were examined by immunoblotting. The statins are arranged in order of decreasing lipophilicity. Ceri, cerivastatin; Simv, simvastatin; Fluv, fluvastatin; Ator, atorvastatin; Rosu, rosuvastatin; Prav, pravastatin. (D) RAW264.7 cells were cultured in the absence (shaded histograms) or presence (10 uM) of fluvastatin (open red histograms) and simvastatin (open blue histograms). Surface levels of CD9, CD63, CD81, and the integrin beta1 subunit were analyzed by flow cytometry.From: Jin Y, Tachibana I, Takeda Y, He P, Kang S, et al. (2013) Statins Decrease Lung Inflammation in Mice by Upregulating Tetraspanin CD9 in Macrophages. PLoS ONE 8(9): e73706.)

Application Data (Published customer image: Density fractionation of EVs. The figure shows sucrose gradients of EVs preparations from MLP29 (A) and RH (B). Aliquots of these fractions were used for RNA extraction and protein extraction; the most abundant transcripts were found in the fractions containing typical exosomal markers (Tsg101 or Aip1). RH preparations showed more diversity, with vesicle populations fractionating at different densities.From: Royo F, Schlangen K, Palomo L, Gonzalez E, Conde-Vancells J, et al. (2013) Transcriptome of Extracellular Vesicles Released by Hepatocytes. PLoS ONE 8(7): e68693.)

Application Data (Published customer image: The anti-inflammatory effects of statins are CD9-dependent. (A) BMDMs from WT mice were cultured for 24 h in the absence (-) or presence of 3 uM fluvastatin (Fluv) (+), and unstimulated (-) or stimulated for 24 h with 1 ug/ml LPS (+). The cells were lysed, and levels of CD9 and CD81 were examined by immunoblotting. Anti-actin blots show that comparable amounts of protein were loaded in each lane. (B) BMDMs from WT and CD9 KO mice were cultured in the absence or presence of the indicated concentrations of fluvastatin, and stimulated for 18 h with 10 ug/ml LPS (+). Activities of MMP-9 in culture supernatants were analyzed by gelatin zymography. (C) BMDMs from WT and CD9 KO mice were cultured in the absence (vehicle) or presence of 10 uM fluvastatin or simvastatin (Simv), and unstimulated (-) or stimulated for 18 h with 1 ug/ml LPS (+). Concentrations of TNF-a in culture supernatants were measured by ELISA. Each bar represents the mean +/- SEM. ?P < 0.05; ? ? P < 0.01.From: Jin Y, Tachibana I, Takeda Y, He P, Kang S, et al. (2013) Statins Decrease Lung Inflammation in Mice by Upregulating Tetraspanin CD9 in Macrophages. PLoS ONE 8(9): e73706.)

Application Data (Published customer image: Statins transfer CD14 from lipid rafts into CD9-enriched microdomains. (A) RAW264.7 cells were stimulated with 0.1 ug/ml LPS and, after the indicated times, the cells were lysed and protein levels were examined by immunoblotting. Anti-actin blots show that comparable amounts of protein were loaded in each lane. (B) RAW264.7 cells were untreated (-) or cultured for 24 h in the absence (-) or presence of 5 uM fluvastatin (Fluv) or simvastatin (Simv) (+) and stimulated for 2 h with 1 ug/ml LPS (+). Proteins in whole-cell lysate (WCL) and CD14 protein in immunoprecipitates (IP) with anti-TLR4 Ab were immunoblotted (IB). (C) RAW264.7 cells were treated as in B. Lysates of untreated (C, control) cultures or LPS-stimulated cultures in the absence (L) or presence of fluvastatin (FL) or simvastatin (SL) were fractionated by sucrose density gradients, and protein distributions were visualized by immunoblotting. The intensities of blots were quantified by densitometry, and percentages of density units of light membrane (LM) fractions are displayed to the right of the blots. Data shown are from one representative of three similar experiments. (D) Immunoblots of CD9 and CD81 proteins in whole-cell lysates and in immunoprecipitates with control IgG or anti-CD14 mAb. (E) Immunoblots of CD9 and CD81 proteins in whole-cell lysates and in immunoprecipitates with control IgG or anti-CD14 mAb from pooled LM fractions (4 and 5) and dense (D) fractions (9 and 10). In the presence of statins, more CD14/CD9 complexes were formed in dense fractions (arrowheads).From: Jin Y, Tachibana I, Takeda Y, He P, Kang S, et al. (2013) Statins Decrease Lung Inflammation in Mice by Upregulating Tetraspanin CD9 in Macrophages. PLoS ONE 8(9): e73706.)

Application Data (Published customer image: Fluvastatin and simvastatin increase CD9 and CD81 levels in RAW264.7 cells.(A) RAW264.7 cells were cultured for 24 h in the absence or presence of increasing concentrations of fluvastatin (Fluv) or simvastatin (Simv). The cells were lysed, and levels of CD9, CD63, and CD81 were examined by immunoblotting. Anti-actin blots show that comparable amounts of protein were loaded in each lane. (B) RAW264.7 cells were untreated (-) or cultured in the absence or presence of increasing concentrations of fluvastatin or simvastatin and stimulated for 24 h with 0.1 ug/ml LPS (+). Levels of CD9, CD63, and CD81 were examined by immunoblotting. Note that LPS downregulates CD9 and CD81 in the absence of statins (arrowheads). (C) RAW264.7 cells were cultured in the absence (-) or presence of 3 uM fluvastatin (+), and unstimulated (-) or stimulated for 24 h with 1 ug/ml LPS (+). mRNA levels of CD9 and CD81 were examined by reverse transcription PCR. GAPDH is an internal loading control. (D) RAW264.7 cells were cultured in the absence or presence of fluvastatin, and unstimulated or stimulated with LPS. Control (Cont) was an untreated culture. mRNA levels of CD9 and CD81 were examined by real-time PCR. Data shown are from one representative of three similar experiments. (E) Human monocytic THP-1 cells were treated for 4 h with 1 ug/ml phorbol 12-myristate 13-acetate, allowed to attach to a plate, and then cultured in the absence or presence of increasing concentrations of simvastatin. Levels of CD9, CD63, and CD81 were examined by immunoblotting. (F) Mouse 3T3 fibroblasts were cultured in the absence or presence of increasing concentrations of simvastatin. Levels of CD9, CD63, and CD81 were examined by immunoblotting.From: Jin Y, Tachibana I, Takeda Y, He P, Kang S, et al. (2013) Statins Decrease Lung Inflammation in Mice by Upregulating Tetraspanin CD9 in Macrophages. PLoS ONE 8(9): e73706.)

Application Data (Staining of mouse spleen cells with Hamster anti Mouse CD81:FITC)

Application Data (Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 647 (AAA11941A647))

Compare (5 max)

CD81, Monoclonal Antibody (Cat# AAA11941)

Full Name

HAMSTER ANTI MOUSE CD81

Gene Names

Cd81; Tapa1; Tapa-1; Tspan28

Reactivity

Rat

Applications

Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)*

Pricing

$560/0.25 mg $1,080/2x0.25 mg $1,570/3x0.25 mg $2,035/4x0.25 mg $2,275/5x0.25 mg

< >

<< Previous Next >>

Application Data (Published clone specific image: Flow cytometric analysis of AM from NO2-exposed and control rats. Rats were exposed to NO2 for the indicated times and BAL cells were stained with antibodies to ED7, ED9, RM-4, and OX-6. To overcome autofluorescence signals, primary antibodies were detected using a biotin-PE/streptavidin-anti-streptavidin enhancing system and labeling of AM was analyzed by flow cytometry following gating by help of forward and sideward scatter properties. Shown are representative results of at least six animals per group.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)

Application Data (Published clone specific image: Flow cytometric analysis of ED7 and ED9 expression of AM following magnetic bead separation. AM of 3 days NO2-exposed rats were separated due to their expression of the cell surface molecule ED7 using magnetic bead separation. Susbsequently, ED7 (left) and ED9 (right) expression was analyzed in unseparated AM (A), ED7-positive AM (B), and ED7-negative AM (C). Numbers right of each histogram represent the mean fluorescence of the respective cell population. The figure clearly demonstrates that ED7-positive AM show a lower ED9 expression compared to ED7-negative AM. Shown is a representative data set of more than twenty animals.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)

Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD172a:RPE)

Compare (5 max)

CD172a, Monoclonal Antibody (Cat# AAA12001)

Full Name

MOUSE ANTI RAT CD172a

Gene Names

Sirpa; Bit; Ptpns1; SHPS-1

Applications

Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)

Pricing

$590/0.25 mg $1,140/2x0.25 mg $1,655/3x0.25 mg $2,145/4x0.25 mg $2,390/5x0.25 mg

< >

<< Previous Next >>

Application Data (Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)

Application Data (Published customer image: Western blot analysis to determine type of activation of adult primary microglial cells produced from wild-type and IL-1 KO mice stimulated with IL-4, IL-13 or IL-4/IL-13 with or without IL-1beta. (A) Representative western blotting data of primary microglial cells produced from wild-type (wild) and IL-1 KO mice and exposed for 24 hours to IL-4, IL-13 or IL-4 plus IL-13 (IL-4/IL-13) with or without IL-1beta. Each lane expected to CD206 blotting were applied 7 ug of reduced samples. Non-reduced samples (5 ug) were applied to detect CD206. Densitometric analysis of COX2 (B), Ym1 (C), Arg-1 (D) and CD206 (E) (n = 3 each group). (B) COX2 levels are increased by exposure of cells to IL-1beta and are not influenced by IL-4 or IL-13 alone. The COX2 level was slightly enhanced by IL-1beta and IL-4 co-treatment. (C) Ym1 levels are increased by exposure of cells to IL-4 and IL-4/Il-13 and are synergistically increased by co-treatment with IL-1beta. However, only a low level of Ym1 is seen upon exposure of cells to IL-13, and is significantly less than that seen in response to exposure of cells to IL-4. (D) Arg-1 shows similar levels in response to exposure to IL-4 and IL-4/IL-13; these are synergistically increased by co-treatment with IL-1beta. However, low levels of Arg-1 are seen for exposure of cells to IL-13. (G) CD206 was detected in response to exposure of cells to both IL-4 and IL-4/IL-13 with or without IL-1beta; however, CD206 levels in IL-13-exposed samples were lower than those seen with the other treatments. Data are expressed as mean +/- SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the IL-4-treated group without IL-1beta for each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX 2, cyclooxygenase 2.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation. 2012 Apr 7;9:65.)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)

Application Data (Staining of J774 cell line with Rat anti Mouse CD206 following permeabilisation with Leucoperm)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 488)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 647)

Compare (5 max)

CD206, Monoclonal Antibody (Cat# AAA12121)

Full Name

RAT ANTI MOUSE CD206:FITC

Gene Names

Mrc1; MR; CD206; AW259686

Applications

Flow cytometry (FC/FACS)*

Pricing

$810/0.5 mg $1,570/2x0.5 mg $2,285/3x0.5 mg $2,960/4x0.5 mg $3,385/5x0.5 mg

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.