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WB (Western Blot) (Western blot analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged GATA2 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GATA2 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody. Lane 1: GATA2 transfected lysate (50.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GATA2 monoclonal antibody, Western Blot analysis of GATA2 expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (36.96kD).)
WB (Western Blot) (Western Blot analysis of ATP6V1G2 expression in transfected 293T cell line by ATP6V1G2 monoclonal antibody. Lane 1: ATP6V1G2 transfected lysate (13.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ATP6V1G2 monoclonal antibody Western Blot analysis of ATP6V1G2 expression in NIH/3T3.)
WB (Western Blot) (ATP6V1G2 monoclonal antibody Western Blot analysis of ATP6V1G2 expression in Raw 264.7.)
WB (Western Blot) (ATP6V1G2 monoclonal antibody, Western Blot analysis of ATP6V1G2 expression in HepG2.)
WB (Western Blot) (ATP6V1G2 monoclonal antibody. Western Blot analysis of ATP6V1G2 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (34.32kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of A549 cells with PRPF8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining PRPF8 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PRPF8 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PRPF8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PRPF8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-PRPF8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PRPF8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PRPF8 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PRPF8 on SiHa (1) and A549 (2) cell lysate using anti-PRPF8 antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells with Cdk7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Cdk7 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cdk7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cdk7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cdk7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cdk7 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cdk7 antibody. Counter stained with hematoxylin.)
IF (Immunofluorescence) (Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence) (Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with U266 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
FCM (Flow Cytometry) (CD138 Antibody (C-term) (Ascites) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)
FCM (Flow Cytometry) (CD138 Antibody (C-term) (Ascites) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)
FCM (Flow Cytometry) (CD138 Antibody (C-term) (Ascites) flow cytometric analysis of U266 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)
WB (Western Blot) (CD138 Antibody (C-term)(Ascites) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CD138 antibody detected the CD138 protein (arrow).)
WB (Western Blot) (Western Blot analysis of CTNNB1 expression in transfected 293T cell line by CTNNB1 monoclonal antibody. Lane 1: CTNNB1 transfected lysate (85.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between FLT1 and CTNNB1. Huh7 cells were stained with FLT1 rabbit purified polyclonal 1:1200 and CTNNB1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between GSK3B and CTNNB1. HeLa cells were stained with GSK3B rabbit purified polyclonal 1:1200 and CTNNB1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
WB (Western Blot) (Western blot analysis of CTNNB1 over-expressed 293 cell line, cotransfected with CTNNB1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CTNNB1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CTNNB1 is 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CTNNB1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (HSPA1B monoclonal antibody (M05), clone 2D11. Western Blot analysis of HSPA1B expression in PC-12.)
WB (Western Blot) (HSPA1B monoclonal antibody (M05), clone 2D11. Western Blot analysis of HSPA1B expression in NIH/3T3.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged HSPA1B is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (HSPA1B monoclonal antibody (M05), clone 2D11 Western Blot analysis of HSPA1B expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Figure 2: Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and lung cancer (right) using p63alpha mouse mAb with DAB staining.)
WB (Western Blot) (Figure 1: Western blot analysis using p63alpha mouse mAb against A431 (1), Hela (2), Jurkat (3), THP-1 (4), NIH/3T3 (5), Cos7 (6) and PC-12 (7) cell lysate.)
WB (Western Blot) (Western blot analysis of PCDH8 over-expressed 293 cell line, cotransfected with PCDH8 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PCDH8 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PCDH8 is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PCDH8 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of PCDH8 expression in transfected 293T cell line by PCDH8 monoclonal antibody. Lane 1: PCDH8 transfected lysate (113kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PCDH8 monoclonal antibody Western Blot analysis of PCDH8 expression in COLO 320 HSR.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
Application Data (Detection limit for recombinant GST tagged MAPK3 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of MAPK3 transfected lysate using MAPK3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAPK3 monoclonal antibody)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MAPK3 on HeLa cell. [antibody concentration 25ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MAPK3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of MAPK3 expression in transfected 293T cell line by MAPK3 monoclonal antibody. Lane 1: MAPK3 transfected lysate (43.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MAPK3 monoclonal antibody Western Blot analysis of MAPK3 expression in A-431.)
FCM (Flow Cytometry) (Flow cytometric analysis of PC-12 cells with HMGB1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining HMGB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining HMGB1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HMGB1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HMGB1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HMGB1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HMGB1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of HMGB1 on different cell lysates using anti-HMGB1 antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: PC12 Lane 3: F9)
WB (Western Blot) (Detection of human STAT3 by western blot. Samples: Whole cell lysate (50 ug) from RKO, NCI-H226, HEK293T, GaMG, LNCaP, HeLa, Ramos, MOLT-4, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation) (Detection of human STAT3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NCI-H226 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1) used for IP at 6 ul per reaction. STAT3 was also immunoprecipitated by rabbit anti-STAT3 antibody For blotting immunoprecipitated STAT3, AAA23832 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry) (Detection of human STAT3 in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of mouse STAT3 in FFPE renal cell carcinoma by IHC. Antibody: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human STAT3 in FFPE H226 cells by ICC. Antibody: Rabbit anti-STAT3 recombinant monoclonal antibody (AAA23832 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human STAT3 (shaded) in Ramos cells by flow cytometry. Antibody: Rabbit anti-STAT3 recombinant monoclonal (AAA23832) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in PC-12.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP3K7 and HSPA1L HeLa cells were stained with MAP3K7 rabbit purified polyclonal 1:1200 and HSPA1L mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged HSPA1L is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1L on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])
WB (Western Blot) (HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (34.65kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between BAD and MAPK8 Mahlavu cells were stained with BAD rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PXN and MAPK8 Huh7 cells were stained with PXN rabbit purified polyclonal 1:1200 and MAPK8 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CRK and MAPK8 HeLa cells were stained with CRK rabbit purified polyclonal 1:1200 and MAPK8 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
WB (Western Blot) (Western blot analysis of MAPK8 over-expressed 293 cell line, cotransfected with MAPK8 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK8 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged MAPK8 is 1ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of MAPK8 expression in transfected 293T cell line by MAPK8 monoclonal antibody. Lane 1: MAPK8 transfected lysate (48.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (DCPS monoclonal antibody. Western Blot analysis of DCPS expression in PC-12.)
Application Data (Detection limit for recombinant GST tagged DCPS is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DCPS on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DCPS on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.5ug/ml])
WB (Western Blot) (DCPS monoclonal antibody Western Blot analysis of DCPS expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (63.18kD).)
WB (Western Blot) (Western blot analysis of UGP2 over-expressed 293 cell line, cotransfected with UGP2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with UGP2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged UGP2 is ~0.1ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of UGP2 expression in transfected 293T cell line by UGP2 monoclonal antibody (M01). Lane 1: UGP2 transfected lysate (56.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (UGP2 monoclonal antibody Western Blot analysis of UGP2 expression in NIH/3T3.)
WB (Western Blot) (UGP2 monoclonal antibody Western Blot analysis of UGP2 expression in Raw 264.7)
WB (Western Blot) (UGP2 monoclonal antibody Western Blot analysis of UGP2 expression in PC-12)
WB (Western Blot) (UGP2 monoclonal antibody Western Blot analysis of UGP2 expression in HeLa)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-LAMP2a antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of LAMP2a on different lysates using anti-LAMP2a antibody at 1/1, 000 dilution. Positive control: Lane 1: Human placenta Lane 2: JAR Lane 3: Human liver)
FCM (Flow Cytometry) (Flow cytometric analysis of Raji cells with PAX5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining PAX5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PAX5 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PAX5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PAX5 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PAX5 on Daudi cells lysates using anti-PAX5 antibody at 1/1, 000 dilution.)
Application Data (Staining of mouse spleen cells with Rat anti Mouse CD3 Epsilon (T3): APC)
Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD3 Epsilon (T3): endotoxin low)
Application Data (Immunoperoxidase staining of Mouse lymph node cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Low power)
Application Data (Immunoperoxidase staining of Mouse spleen cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Medium power)
Application Data (Immunoperoxidase staining of Mouse lymph node cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Low power)
Application Data (Immunoperoxidase staining of Mouse spleen cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Low power)
Application Data (Immunoperoxidase staining of Mouse lymph node cryosection with Rat anti Mouse CD3 antibody, clone KT3 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Low power)
Application Data (Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa Fluor 750)
Application Data (Staining of mouse spleen cells with Rat anti Mouse CD3 Epsilon (T3):RPE)
Application Data (Staining of mouse spleen with Rat anti Mouse CD3 Epsilon (T3): RPE - Alexa Fluor 647)
WB (Western Blot) (LIMK1 monoclonal antibody. Western Blot analysis of LIMK1 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
Application Data (Detection limit for recombinant GST tagged LIMK1 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LIMK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])
WB (Western Blot) (LIMK1 monoclonal antibody. Western Blot analysis of LIMK1 expression in NIH/3T3.)
WB (Western Blot) (LIMK1 monoclonal antibody. Western Blot analysis of LIMK1 expression in Raw 264.7.)
WB (Western Blot) (LIMK1 monoclonal antibody Western Blot analysis of LIMK1 expression in SW-13.)
WB (Western Blot) (G3BP monoclonal antibody Western Blot analysis of G3BP expression in A-431)
WB (Western Blot) (Western blot analysis of G3BP over-expressed 293 cell line, cotransfected with G3BP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with G3BP monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to G3BP on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to G3BP on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 1ug/ml])
WB (Western Blot) (Western Blot analysis of G3BP expression in transfected 293T cell line by G3BP monoclonal antibody Lane 1: G3BP transfected lysate (52.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
G3BP1 (Ras GTPase-activating Protein-binding Protein 1, G3BP-1, ATP-dependent DNA Helicase VIII, hDH VIII, GAP SH3 Domain-binding Protein 1, G3BP) (FITC)
Gene Names
G3BP1; G3BP; HDH-VIII
Reactivity
Human
Applications
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
FCM (Flow Cytometry) (Flow cytometric analysis of N2A cells with EIF2C3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining EIF2C3 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining EIF2C3 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining EIF2C3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining EIF2C3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-EIF2C3 antibody. Counter stained with hematoxylin.)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. Med power)
Application Data (Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Medium power)
Application Data (Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Low power)
Application Data (Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. High power)
Application Data (Staining of rat splenocytes with Mouse anti Rat CD43: FITC)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. High power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. Low power)
WB (Western Blot) (RRAS2 monoclonal antibody Western Blot analysis of RRAS2 expression in A-431.)
WB (Western Blot) (RRAS2 monoclonal antibody Western Blot analysis of RRAS2 expression in NIH/3T3)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RRAS2 on A-431 cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RRAS2 on formalin-fixed paraffin-embedded human dysgerminoma tissue. [antibody concentration 5ug/ml])
WB (Western Blot) (Western Blot analysis of RRAS2 expression in transfected 293T cell line by RRAS2 monoclonal antibody Lane 1: RRAS2 transfected lysate (Predicted MW: 23.4kD. Lane 2: Non-transfected lysate.)
WB (Western Blot) (RRAS2 monoclonal antibody Western Blot analysis of RRAS2 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (48.55kD).)
WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)
Application Data (Detection limit for recombinant GST tagged MCM2 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of MCM2 transfected lysate using MCM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MCM2 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM2 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody. Lane 1: MCM2 transfected lysate (101.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MCM2 monoclonal antibody Western Blot analysis of MCM2 expression in HeLa NE.)
WB (Western Blot) (BST2 monoclonal antibody (M15), clone 2E6. Western Blot analysis of BST2 expression in human placenta.)
WB (Western Blot) (BST2 monoclonal antibody (M15), clone 2E6. Western Blot analysis of BST2 expression in HeLa.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BST2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BST2 on HeLa cell. [antibody concentration 10 ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation of BST2 transfected lysate using anti-BST2 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with BST2 MaxPab rabbit polyclonal antibody.)
Application Data (Detection limit for recombinant GST tagged BST2 is 0.1 ng/ml as a capture antibody.)
WB (Western Blot) (Western blot analysis of GSR over-expressed 293 cell line, cotransfected with GSR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GSR monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged GSR is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GSR on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 6ug/ml].)
WB (Western Blot) (Western Blot analysis of GSR expression in transfected 293T cell line by GSR monoclonal antibody. Lane 1: GSR transfected lysate (56.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GSR monoclonal antibody Western Blot analysis of GSR expression in IMR-32.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained 293 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of 293 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained 293 cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.)
FCM (Flow Cytometry) (FACS analysis of HeLa cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained HeLa cells (Black) as negative control.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma using AAA26910(left) or beta-Actin Blocking Peptide (right).)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using AAA26910.)
IF (Immunofluorescence) (Confocal immunofluorescent analysis of COS-7 cells using AAA26910 (green). Blue pseudocolor= DRAQ5 fluorescent DNA dye)
FCM (Flow Cytometry) (Flow cytometric analysis of NIH/3T3 cells using AAA26910 (blue) compared to a nonspecific negative control antibody (red).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human leiomyoma using AAA26910)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human heart using AAA26910. Note the lack of staining of cardiac actin.)
WB (Western Blot) (Western blot analysis of cell extracts from various cell lines using AAA26910.)
ICC (Immunocytochemistry) (ICC staining p53DINP1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining p53DINP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-p53DINP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-p53DINP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded huamn liver cancer tissue using anti-p53DINP1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of p53DINP1 on different cell lysates using anti-p53DINP1 at 1/500 dilution. Positive control�� Line 1: SH-SY-5Y Line 2: HepG2 Line 3: Mouse stomach Line 3: Human stomach)
WB (Western Blot) (Detection of human CD206/Mannose Receptor by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MUTZ-3, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation) (Detection of human CD206/Mannose Receptor by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MUTZ-3 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1) used for IP at 6 ul per reaction. CD206/Mannose Receptor was also immunoprecipitated by rabbit anti-CD206/Mannose Receptor antibody 1507A-3G8. For blotting immunoprecipitated CD206/Mannose Receptor, AAA23836 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry) (Detection of human CD206/Mannose Receptor in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human CD206/Mannose Receptor in FFPE non-Hodgkin's lymphoma by IHC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human CD206/Mannose Receptor in FFPE lung carcinoma pleural effusion by IHC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human CD206/Mannose Receptor in FFPE MUTZ-3 cells by ICC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Application Data (Detection limit for recombinant GST tagged PEPD is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PEPD transfected lysate using PEPD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PEPD rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PEPD on HepG2 cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of PEPD expression in transfected 293T cell line by PEPD monoclonal antibody. Lane 1: PEPD transfected lysate (Predicted MW: 54.6kD). Lane 2: Non-transfected lysate)
WB (Western Blot) (PEPD monoclonal antibody Western Blot analysis of PEPD expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (79.97kD).)
WB (Western Blot) (LEPRE1 monoclonal antibody, Western Blot analysis of LEPRE1 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged LEPRE1 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LEPRE1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LEPRE1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 6ug/ml].)
WB (Western Blot) (Western Blot analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 monoclonal antibody. Lane 1: LEPRE1 transfected lysate (46kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
Application Data (Detection limit for recombinant GST tagged GLUD2 is 0.3 ng/ml as a capture antibody.)
WB (Western Blot) (GLUD2 monoclonal antibody (M01), clone 3C2. Western Blot analysis of GLUD2 expression in human pancreas.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GLUD2 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 1 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GLUD2 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 1 ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation of GLUD2 transfected lysate using anti-GLUD2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GLUD2 MaxPab rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of GLUD2 expression in transfected 293T cell line by GLUD2 monoclonal antibody (M01), clone 3C2.Lane 1: GLUD2 transfected lysate (61 KDa).Lane 2: Non-transfected lysate.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Scavenging Receptor SR-BI antibody (AAA27932). Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Scavenging Receptor SR-BI antibody (AAA27932). Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Scavenging Receptor SR-BI antibody (AAA27932). Counter stained with hematoxylin.)
ICC (Immunocytochemistry) (Immunocytochemistry staining Scavenging Receptor SR-BI in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (Immunocytochemistry staining Scavenging Receptor SR-BI in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot) (Western blot analysis of Scavenging Receptor SR-BI on different lysates using anti-Scavenging Receptor SR-BI antibody (AAA27932) at 1/1,000 dilution.Positive control:Lane 1: Human liverLane 2: Mouse liver)
FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with GAPDH antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining GAPDH in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining GAPDH in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-GAPDH antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GAPDH antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GAPDH antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/1, 000 dilution. Positive control: Lane 1: NCCIT Lane 2: PC12 Lane 3: F9)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using UBE2I mouse mAb with DAB staining.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded prostate tissues using UBE2I mouse mAb with DAB staining.)
FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells using UBE2I mouse mAb (green) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells using UBE2I mouse mAb (green). Blue)
WB (Western Blot) (Western blot analysis using UBE2I mouse mAb against Hela (1), HepG2 (2), and Cos7 (3) cell lysate.)
WB (Western Blot) (Western blot detection of Ubc9 in C6, Jurkat, K562, 3T3 and Hela cell lysates using Ubc9 mouse mAb (1:2000 diluted).Predicted band size:18KDa.Observed band size:17KDa.)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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