Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
Application Data (Detection limit for recombinant GST tagged RPS5 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS5 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of RPS5 expression in transfected 293T cell line by RPS5 monoclonal antibody. Lane 1: RPS5 transfected lysate (22.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (RPS5 monoclonal antibody. Western Blot analysis of RPS5 expression in NIH/3T3.)
WB (Western Blot) (RPS5 monoclonal antibody. Western Blot analysis of RPS5 expression in Raw 264.7.)
WB (Western Blot) (RPS5 monoclonal antibody. Western Blot analysis of RPS5 expression in PC-12.)
WB (Western Blot) (RPS5 monoclonal antibody, Western Blot analysis of RPS5 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged MAPK3 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of MAPK3 transfected lysate using MAPK3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MAPK3 monoclonal antibody)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MAPK3 on HeLa cell. [antibody concentration 25ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MAPK3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of MAPK3 expression in transfected 293T cell line by MAPK3 monoclonal antibody. Lane 1: MAPK3 transfected lysate (43.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MAPK3 monoclonal antibody Western Blot analysis of MAPK3 expression in A-431.)
Application Data (Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (HDAC1 monoclonal antibody (M06), clone 1D6 Western Blot analysis of HDAC1 expression in Hela S3 NE.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (YY1 monoclonal antibody (M04), clone 2C5 Western Blot analysis of YY1 expression in Hela S3 NE.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 7 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 7 ug/ml])
IF (Immunofluorescence) (Immunofluorescence to CDKN1B on HeLa cell using (10ug/ml).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between AKT1 and CDKN1B HeLa cells were stained with AKT1 rabbit purified polyclonal (1:1200) and (1:50). Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
IHC (Immunohistochemistry) (Immunoperoxidase to CDKN1B on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue using (5ug/ml).)
WB (Western Blot) (Western Blot analysis of CDKN1B expression in transfected 293T cell line by Lane 1: CDKN1B transfected lysate (22.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (47.89kD).)
Application Data (Detection limit for is 10ng/ml as a capture antibody.)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with gamma Tubulin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining gamma Tubulin in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining gamma Tubulin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining gamma Tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-gamma Tubulin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-gamma Tubulin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of gamma Tubulin on different lysates using anti-gamma Tubulin antibody at 1/1, 000 dilution. Positive control: Lane 1: A431 Lane 2: Jurkat Lane 3: Mouse brain)
WB (Western Blot) (Detection of human CD28 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, Jurkat, HeLa, RPMI-8226, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot) (Detection of mouse CD28 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, EL4, CH27, RAW 264.7, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation) (Detection of human CD28 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865 lot 1) used for IP at 6 ul/mg lysate. CD28 was also immunoprecipitated by a second antibody against a different epitope of CD28 . For blotting immunoprecipitated CD28, AAA23865 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry) (Detection of human CD28 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry) (Detection of mouse CD28 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human CD28 by immunocytochemistry. Sample: FFPE section of jurkat cells. Antibody: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of mouse CD28 by immunocytochemistry. Sample: FFPE section of EL4 cells. Antibody: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of human CD28 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (LETM1 monoclonal antibody Western Blot analysis of LETM1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.62kD).)
Application Data (Detection limit for recombinant GST tagged LETM1 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of LETM1 transfected lysate using LETM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LETM1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LETM1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LETM1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of LETM1 expression in transfected 293T cell line by LETM1 monoclonal antibody. Lane 1: LETM1 transfected lysate (83.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (FGR monoclonal antibody (M02), clone 3B11. Western Blot analysis of FGR expression in Raw 264.7 (Cat # L024V1).)
WB (Western Blot) (FGR monoclonal antibody (M02), clone 3B11. Western Blot analysis of FGR expression in PC-12 (Cat # L012V1).)
Application Data (Detection limit for recombinant GST tagged FGR is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (FGR monoclonal antibody (M02), clone 3B11 Western Blot analysis of FGR expression in HeLa (Cat # L013V1).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TIMP2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml])
WB (Western Blot) (TIMP2 monoclonal antibody (M03), clone 1C3 Western Blot analysis of TIMP2 expression in HeLa (Cat # L013V1).)
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Purity
Purified
Pricing
SDS-PAGE (Under non-reducing SDS-PAGE conditions, the anti 2019-nCov spike protein S2 domain mAb migrates as a 150kDa protein (Lane NR). Under DTT-reducing conditions it migrates as 50kDa and 25kDa polypeptides (Lane R).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Bax antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Bax antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Bax antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Bax antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Bax antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Bax antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bax antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Bax on different lysates using anti-Bax antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: Human liver)
WB (Western Blot) (Detection of mouse FOX2/RBM9 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody (AAA23868 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot) (Detection of human FOX2/RBM9 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody (AAA23868 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation) (Detection of human FOX2/RBM9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody (AAA23868 lot 1) used for IP at 20 ul/mg lysate. FOX2/RBM9 was also immunoprecipitated by an antibody against a different epitope of FOX2/RBM9 (BL2440) and a polyclonal antibody against the same epitope of FOX2/RBM9 . For blotting immunoprecipitated FOX2/RBM9, AAA23868 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistchemistry) (Detection of mouse FOX2/RBM9 by immunohistochemistry. Sample: FFPE section of RENCA Tumor. Antibody: Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody (AAA23868-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry) (Detection of human FOX2/RBM9 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody (AAA23868-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of mouse FOX2/RBM9 by immunocytochemistry. Sample: FFPE section of NIH 3T3 cells. Antibody: Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody (AAA23868-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human FOX2/RBM9 by immunocytochemistry. Sample: FFPE section of GaMG cells. Antibody: Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody (AAA23868-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of mouse FOX2/RBM9 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody (AAA23868) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of human FOX2/RBM9 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-FOX2/RBM9 recombinant monoclonal antibody (AAA23868) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TESK2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.7 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.7 ug/ml])
WB (Western Blot) (Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M08), clone 5G1.Lane 1: TESK2 transfected lysate(60.3 KDa).Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged SMAD2 is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (SMAD2 monoclonal antibody (M05), clone 3G9 Western Blot analysis of SMAD2 expression in Hela S3 NE (Cat # L013V3).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD2 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMAD2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 5 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to SMAD2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 5 ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation analysis of 300 ug extracts from 293T cells using 3 ug GRB2 antibody (AAA28476). Western blot was performed from the immunoprecipitate using GRB2 (AAA28476) at a dilution of 1:1000.)
ICC (Immunocytochemistry) (Confocal imaging of HeLa cells using GRB2 Rabbit mAb (AAA28476,dilution 1:100)(Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat spleen using GRB2 Rabbit mAb (AAA28476) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil using GRB2 Rabbit mAb (AAA28476) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human breast cancer using GRB2 Rabbit mAb (AAA28476) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using GRB2 Rabbit mAb (AAA28476) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)
WB (Western Blot) (Western blot analysis of TAF7 over-expressed 293 cell line, cotransfected with TAF7 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TAF7 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged TAF7 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TAF7 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of TAF7 expression in transfected 293T cell line by TAF7 monoclonal antibody. Lane 1: TAF7 transfected lysate (40.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TAF7 monoclonal antibody, Western Blot analysis of TAF7 expression in MCF-7.)
WB (Western Blot) (MAN1B1 monoclonal antibody Western Blot analysis of MAN1B1 expression in LNCaP.)
Application Data (Detection limit for recombinant GST tagged MAN1B1 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MAN1B1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])
WB (Western Blot) (MAN1B1 monoclonal antibody Western Blot analysis of MAN1B1 expression in A-431.)
WB (Western Blot) (MAN1B1 monoclonal antibody Western Blot analysis of MAN1B1 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (GLMN monoclonal antibody Western Blot analysis of GLMN expression in HL-60.)
WB (Western Blot) (GLMN monoclonal antibody Western Blot analysis of GLMN expression in Jurkat.)
Application Data (Detection limit for recombinant GST tagged GLMN is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of GLMN transfected lysate using GLMN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GLMN rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GLMN on HeLa cell. [antibody concentration 20ug/ml])
WB (Western Blot) (Western Blot analysis of GLMN expression in transfected 293T cell line by GLMN monoclonal antibody. Lane 1: GLMN transfected lysate (68.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (91.45kD).)
WB (Western Blot) (DCPS monoclonal antibody. Western Blot analysis of DCPS expression in PC-12.)
Application Data (Detection limit for recombinant GST tagged DCPS is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DCPS on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DCPS on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.5ug/ml])
WB (Western Blot) (DCPS monoclonal antibody Western Blot analysis of DCPS expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (63.18kD).)
Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (YY1 monoclonal antibody (M03), clone 4D2 Western Blot analysis of YY1 expression in Hela S3 NE.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml])
ICC (Immunocytochemistry) (ICC staining FUBP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining FUBP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining FUBP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-FUBP1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of FUBP1 on different lysates using anti-FUBP1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Raji)
ICC (Immunocytochemistry) (ICC staining Histone H2B (mono methyl R79) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Histone H2B (mono methyl R79) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Histone H2B (mono methyl R79) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B (mono methyl R79) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B (mono methyl R79) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B (mono methyl R79) antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western Blot detection against Immunogen (60.94kD).)
IP (Immunoprecipitation) (Immunoprecipitation of ANXA5 transfected lysate using ANXA5 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ANXA5 rabbit polyclonal antibody.)
Application Data (Detection limit for recombinant GST tagged ANXA5 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ANXA5 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ANXA5 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ANXA5 expression in transfected 293T cell line by ANXA5 monoclonal antibody. Lane 1: ANXA5 transfected lysate (35.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ANXA5 monoclonal antibody, Western Blot analysis of ANXA5 expression in Hela.)
WB (Western Blot) (TRIM33 monoclonal antibody, Western Blot analysis of TRIM33 expression in Hela NE)
WB (Western Blot) (TRIM33 monoclonal antibody. Western Blot analysis of TRIM33 expression in 293)
Application Data (Detection limit for recombinant GST tagged TRIM33 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TRIM33 on HeLa cell. [antibody concentration 30ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TRIM33 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])
WB (Western Blot) (TRIM33 monoclonal antibody. Western Blot analysis of TRIM33 expression in PC-12)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in PC-12.)
WB (Western Blot) (MGC21874 monoclonal antibody, Western Blot analysis of MGC21874 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged MGC21874 is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MGC21874 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5ug/ml].)
WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in NIH/3T3.)
WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in Raw 264.7.)
WB (Western Blot) (Western Blot detection against Immunogen (38.1kD).)
WB (Western Blot) (HHIP monoclonal antibody. Western Blot analysis of HHIP expression in MCF-7.)
WB (Western Blot) (HHIP monoclonal antibody, Western Blot analysis of HHIP expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged HHIP is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HHIP transfected lysate using HHIP monoclonal antibody and Protein A Magnetic Bead and immunoblotted with HHIP rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HHIP on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of HHIP expression in transfected 293T cell line by HHIP monoclonal antibody. Lane 1: HHIP transfected lysate (78.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (EHD3 monoclonal antibody. Western Blot analysis of EHD3 expression in COLO 320 HSR.)
WB (Western Blot) (EHD3 monoclonal antibody, Western Blot analysis of EHD3 expression in IMR-32.)
Application Data (Detection limit for recombinant GST tagged EHD3 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EHD3 on HeLa cell. [antibody concentration 25ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EHD3 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)
WB (Western Blot) (EHD3 monoclonal antibody. Western Blot analysis of EHD3 expression in MCF-7.)
WB (Western Blot) (Western Blot detection against Immunogen (31.61kD).)
Application Data (Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (HDAC1 monoclonal antibody (M06), clone 1D6 Western Blot analysis of HDAC1 expression in Hela S3 NE.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (YY1 monoclonal antibody (M04), clone 2C5 Western Blot analysis of YY1 expression in Hela S3 NE (Cat # L013V3).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 7 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 7 ug/ml])
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CASP3 antibody.)
IF (Immunofluorescence) (Immunofluorescence analysis of PC-3M cells using CASP3 antibody.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using CASP3 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using CASP3 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human spleen using CASP3 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of Jurkat cells, using CASP3 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
ICC (Immunocytochemistry) (ICC staining Phospho-Smad5 (S463/465) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-Smad5 (S463/465) in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-Smad5 (S463/465) antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Phospho-Smad5 (S463/465) on different lysates using anti-Phospho-Smad5 (S463/465) antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Rat brain)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with PCNA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-PCNA antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PCNA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinama tissue using anti-PCNA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PCNA on different lysates using anti-PCNA antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: A431)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with PDGFR alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining PDGFR alpha in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PDGFR alpha in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PDGFR alpha antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PDGFR alpha on NIH/3T3 cells lysates using anti-PDGFR alpha antibody at 1/1, 000 dilution.)
WB (Western Blot) (Detection of human Neurofilament-L by western blot. Samples: Whole cell lysate (25 ug) from HEK293T, HeLa, SK-N-BE(2), Jurkat, and 786-O cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot) (Detection of mouse and rat Neurofilament-L by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, B35, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation) (Detection of human Neurofilament-L by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874 lot 1) used for IP at 20 ul/mg lysate. Neurofilament-L was also immunoprecipitated by a second antibody against a different epitope of Neurofilament-L (BL-2033B-1C7). For blotting immunoprecipitated Neurofilament-L, AAA23874 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry) (Detection of human Neurofilament-L by immunohistochemistry. Sample: FFPE section of human medulla. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry) (Detection of mouse Neurofilament-L by immunohistochemistry. Sample: FFPE section of mouse cerebellum. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of rat Neurofilament-L by immunocytochemistry. Sample: FFPE section of B35 cells. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human Neurofilament-L by immunocytochemistry. Sample: FFPE section of SK-N-BE(2) cells. Antibody: Rabbit anti-Neurofilament-L recombinant monoclonal antibody (AAA23874-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
Application Data (Detection limit for 123091 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence to AHR on HeLa cell using 123091 (10ug/ml).)
IP (Immunoprecipitation) (Immunoprecipitation of AHR transfected lysate using 123091 and Protein A Magnetic Bead and immunoblotted with AHR rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with 123091. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot detection against immunogen (36.74kD).)
WB (Western Blot) (RRAS2 monoclonal antibody Western Blot analysis of RRAS2 expression in A-431.)
WB (Western Blot) (RRAS2 monoclonal antibody Western Blot analysis of RRAS2 expression in NIH/3T3)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RRAS2 on A-431 cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RRAS2 on formalin-fixed paraffin-embedded human dysgerminoma tissue. [antibody concentration 5ug/ml])
WB (Western Blot) (Western Blot analysis of RRAS2 expression in transfected 293T cell line by RRAS2 monoclonal antibody Lane 1: RRAS2 transfected lysate (Predicted MW: 23.4kD. Lane 2: Non-transfected lysate.)
WB (Western Blot) (RRAS2 monoclonal antibody Western Blot analysis of RRAS2 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (48.55kD).)
MARCH7 (E3 Ubiquitin-protein Ligase MARCH7, Axotrophin, Membrane-associated RING Finger Protein 7, Membrane-associated RING-CH Protein VII, MARCH-VII, RING Finger Protein 177, AXOT, RNF177, DKFZp586F1122) (FITC)
Gene Names
MARCH7; AXO; AXOT; RNF177; MARCH-VII
Reactivity
Human, Mouse, Rat
Applications
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
WB (Western Blot) (LEPRE1 monoclonal antibody, Western Blot analysis of LEPRE1 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged LEPRE1 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LEPRE1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LEPRE1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 6ug/ml].)
WB (Western Blot) (Western Blot analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 monoclonal antibody. Lane 1: LEPRE1 transfected lysate (46kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between STAT1 and PDGFRB Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal (1:1200) and (1:50). Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged PDGFRB is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PDGFRB transfected lysate using and Protein A Magnetic Bead, and immunoblotted with PDGFRB rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human uterus myoma using.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human stomach using.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IP (Immunoprecipitation) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)
ELISA (Figure 5. ELISA plate was coated with recombinant BCMA-hFc fusion protein (PME100001), followed by pre-blocking with huC11D5.3 antibody (Grey bar) or rabbit control IgG (Black bar), and then different rabbit DimAbs antibodies were added to check the competitive inhibition of huC11D5.3. DM3 clone exhibits the strongest inhibition (Red bar). This data indicated that DM3 bind to the same epitope as bb2121.)
Application Data (Figure 4. Affinity ranking of different DimAb clones by titration of rabbit DimAb antibody concentration onto K562-BCMA or NCI-H929 cells. Different concentrations of various anti-BCMA DimAb clones were incubated with K562-BCMA (A) or NCI-H929 cells (B) at 4 degree C. Bound rabbit IgG was detected in flow cytometry analysis. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Application Data (Figure 3. Phylogenetic analysis of different Anti-BCMA DimAb clones. A) heavy chain and B) Light chain.)
FCM (Flow Cytometry) (Figure 2. A. Flow cytometry analysis with anti-BCMA (DM3) on NCI-H929 cells (Red histogram) or rabbit control antibody on NCI-H929 cells (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM3) on NCI-H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
FCM (Flow Cytometry) (Figure 1. A. Flow cytometry analysis with anti-BCMA (DM3) on K562-BCMA (Red histogram) (K562 cells stably transduced by human BCMA full length gene) and K562 (Negative control cell line) (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM3). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with CD99 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining CD99 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD99 in PANC-1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD99 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD99 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD99 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CD99 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CD99 on THP-1 cells lysates using anti-CD99 antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of 293T cells with Syntaxin 3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Syntaxin 3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Syntaxin 3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Syntaxin 3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Syntaxin 3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Syntaxin 3 on SH-SY-5Y cell (1) and human kidney tissue (2) lysate using anti-Syntaxin 3 antibody at 1/1, 000 dilution.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Vinculin Monoclonal Antibody at dilution of 1:200.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Vinculin Monoclonal Antibody at dilution of 1:200.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Vinculin Monoclonal Antibody at dilution of 1:200.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colon cancer using Vinculin Monoclonal Antibody at dilution of 1:1000.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer using Vinculin Monoclonal Antibody at dilution of 1:1000.)
WB (Western Blot) (Western blot with Anti Vinculin Monoclonal Antibody at dilution of 1:3000. Lane 1: HeLa cell lysate, Lane 2: 293 cell lysate, Lane 3: Hep G2 cell lysate, Lane 4: NIH/3T3 cell lysate, Lane 5: Mouse heart tissue lysate, Lane 6: Rat heart tissue lysate.)
WB (Western Blot) (Detection of human TCF1/TCF7 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Jurkat, HEK293T, MOLT-4, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody (AAA23875 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot) (Detection of human TCF1/TCF7 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN lysis buffer from MOLT4 cells (WT and KO). Antibody: Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody (AAA23875 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot) (Detection of mouse TCF1/TCF7 by western blot. Samples: Whole cell lysate (50 ug) from EL4, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody (AAA23875 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IHC (Immunohistochemistry) (Detection of human TCF1/TCF7 by immunohistochemistry. Sample: FFPE section of human lymph node. Antibody: Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody (AAA23875-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry) (Detection of mouse TCF1/TCF7 by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody (AAA23875-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of mouse TCF1/TCF7 by immunocytochemistry. Sample: FFPE section of EL4 cells. Antibody: Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody (AAA23875-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human TCF1/TCF7 by immunocytochemistry. Sample: FFPE sections of MOLT4 cells (left) and MOLT4 knock-out cells (right). Antibody: Rabbit anti-TCF1/TCF7 recombinant monoclonal antibody (AAA23875-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Purity
Purified
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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