Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Argonaute 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Argonaute 2 in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Argonaute 2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Argonaute 2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Argonaute 2 on different lysates using anti-Argonaute 2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7)

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Argonaute 2, Monoclonal Antibody (Cat# AAA30277)

Full Name

Argonaute 2 Antibody

Gene Names

AGO2; Q10; EIF2C2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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WB (Western Blot) (Detection of mouse CD79A by western blot. Samples: Whole cell lysate (50 ug) from WEHI-231 (25 ug), NIH 3T3, CH27, TCMK-1, and A20 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot) (Detection of human CD79A by western blot. Samples: Whole cell lysate (50 ug) from Daudi, HeLa, Raji, HEK293T, and Ramos cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation) (Detection of human CD79A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Ramos cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878 lot 1) used for IP at 20 ul/mg lysate. CD79A was also immunoprecipitated by a second antibody against a different epitope of CD79A . For blotting immunoprecipitated CD79A, AAA23878 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohistchemistry) (Detection of mouse CD79A by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry) (Detection of human CD79A by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of human CD79A by immunocytochemistry. Sample: FFPE section of Daudi cells. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of mouse CD79A by immunocytochemistry. Sample: FFPE section of WEHI-231 cells. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human CD79A (shaded) in Daudi cells by flow cytometry. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of mouse CD79A (shaded) in A20 cells by flow cytometry. Antibody: Rabbit anti-CD79A recombinant monoclonal antibody (AAA23878) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

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CD79A, Monoclonal Recombinant Antibody (Cat# AAA23878)

Full Name

Rabbit anti-CD79A Recombinant Monoclonal Antibody [BLR234K]

Gene Names

CD79A; IGA; MB-1

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

Purity

Purified

Pricing

$265/0.01 mL $640/0.1 mL $930/5x0.01 mL $1,235/2x0.1 mL $1,800/3x0.1 mL $2,330/4x0.1 mL $2,635/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged GTF2H1 is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GTF2H1 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.[antibody concentration 5ug/ml])

WB (Western Blot) (Western Blot analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody. Lane 1: GTF2H1 transfected lysate (62kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GTF2H1 monoclonal antibody Western Blot analysis of GTF2H1 expression in Jurkat.)

WB (Western Blot) (GTF2H1 monoclonal antibody Western Blot analysis of GTF2H1 expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (86.02kD).)

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GTF2H1, Monoclonal Antibody (Cat# AAA25414)

Full Name

GTF2H1 (BTF2, General Transcription Factor IIH Subunit 1, Basic Transcription Factor 2 62kD Subunit, General Transcription Factor IIH Polypeptide 1, TFIIH Basal Transcription Factor Complex p62 Subunit) (HRP)

Gene Names

GTF2H1; P62; BTF2; TFB1; TFIIH

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PGR is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (PGR monoclonal antibody (M08), clone 4E9 Western Blot analysis of PGR expression in MCF-7.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGR on MCF-7 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGR on MCF-7 cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGR on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to PGR on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3 ug/ml])

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PGR, Monoclonal Antibody (Cat# AAA25926)

Full Name

PGR (Progesterone Receptor, NR3C3, PR) (AP)

Gene Names

PGR; PR; NR3C3

Applications

Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (Detection limit for recombinant GST tagged TESK2 is approximately 0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.7 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.7 ug/ml])

WB (Western Blot) (Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M08), clone 5G1.Lane 1: TESK2 transfected lysate(60.3 KDa).Lane 2: Non-transfected lysate.)

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TESK2, Monoclonal Antibody (Cat# AAA26182)

Full Name

TESK2 (Testis-Specific Kinase 2) (Biotin)

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (TBX3 monoclonal antibody (M02), clone 8H3. Western Blot analysis of TBX3 expression in PC-12 (Cat # L012V1).)

WB (Western Blot) (TBX3 monoclonal antibody (M02), clone 8H3. Western Blot analysis of TBX3 expression in NIH/3T3 (Cat # L018V1).)

Application Data (Detection limit for recombinant GST tagged TBX3 is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (TBX3 monoclonal antibody (M02), clone 8H3 Western Blot analysis of TBX3 expression in IMR-32 (Cat # L008V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TBX3 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TBX3 on HeLa cell. [antibody concentration 10 ug/ml])

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TBX3, Monoclonal Antibody (Cat# AAA26438)

Full Name

TBX3 (T-Box 3, TBX3-ISO, UMS, XHL) (HRP)

Gene Names

TBX3; UMS; XHL; TBX3-ISO

Applications

Immunofluorescence (IF), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Proximity Ligation Analysis of protein-protein interactions between STAT1 and PDGFRB Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal (1:1200) and (1:50). Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged PDGFRB is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of PDGFRB transfected lysate using and Protein A Magnetic Bead, and immunoblotted with PDGFRB rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of PDGFRB expression in human uterus myoma using.)

WB (Western Blot) (Western Blot analysis of PDGFRB expression in human stomach using.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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PDGFRB, Monoclonal Antibody (Cat# AAA26694)

Full Name

PDGFRB (PDGFR, PDGFR1, Platelet-derived Growth Factor Receptor beta, Beta Platelet-derived Growth Factor Receptor, Beta-type Platelet-derived Growth Factor Receptor, CD140 Antigen-like Family Member B, Platelet-derived Growth Factor Receptor 1, CD140b) (M

Gene Names

PDGFRB; IMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1

Reactivity

Human

Applications

FLISA, Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using RXR? antibody (AAA28486) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

IP (Immunoprecipitation) (Immunoprecipitation analysis of 300 ug extracts from HepG2 cells using 3 ug RXR? antibody (AAA28486). Western blot was performed from the immunoprecipitate using RXR? antibody (AAA28486) at a dilution of 1:1000.)

ICC (Immunocytochemistry) (Confocal imaging of HeLa cells using RXR? Rabbit mAb (AAA28486,dilution 1:100)(Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.)

WB (Western Blot) (Western blot analysis of various lysates using RXR? Rabbit mAb (AAA28486) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 90s.)

WB (Western Blot) (Western blot analysis of various lysates using RXR? Rabbit mAb (AAA28486) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)

ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of RXR? Rabbit mAb (AAA28486). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of RXR? in the representative genomic region surrounding ECH1 gene.)

ChIP (Chromatin Immunoprecipitation) (Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of RXR? Rabbit mAb (AAA28486). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of RXR? across chromosome 19 (upper panel) and the genomic region encompassing ECH1, a representative gene enriched in RXR? (lower panel).)

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RXRalpha, Monoclonal Antibody (Cat# AAA28486)

Full Name

RXRalpha Rabbit mAb

Reactivity

Human

Applications

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA (EIA), Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation (ChIP)-seq

Purity

Affinity purification

Pricing

$270/0.02 mL $470/0.1 mL $905/2x0.1 mL $1,315/3x0.1 mL $1,700/4x0.1 mL $1,870/5x0.1 mL

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WB (Western Blot) (AGTRAP monoclonal antibody, Western Blot analysis of AGTRAP expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged AGTRAP is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AGTRAP on formalin-fixed paraffin-embedded human prostate. [antibody concentration 0.8ug/ml].)

WB (Western Blot) (Western Blot analysis of AGTRAP expression in transfected 293T cell line by AGTRAP monoclonal antibody. Lane 1: AGTRAP transfected lysate (16.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western blot analysis of AGTRAP over-expressed 293 cell line, cotransfected with AGTRAP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with AGTRAP monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

WB (Western Blot) (Western Blot detection against Immunogen (31.83kD).)

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AGTRAP, Monoclonal Antibody (Cat# AAA24129)

Full Name

AGTRAP (ATRAP, Type-1 Angiotensin II Receptor-associated Protein, AT1 Receptor-associated Protein, MGC29646) (AP)

Gene Names

AGTRAP; ATRAP

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged RPS7 is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS7 on HeLa cell. [antibody concentration 20ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RPS7 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])

WB (Western Blot) (RPS7 monoclonal antibody Western Blot analysis of RPS7 expression in NIH/3T3.)

WB (Western Blot) (RPS7 monoclonal antibody. Western Blot analysis of RPS7 expression in Raw 264.7.)

WB (Western Blot) (RPS7 monoclonal antibody. Western Blot analysis of RPS7 expression in PC-12.)

WB (Western Blot) (RPS7 monoclonal antibody Western Blot analysis of RPS7 expression in HeLa.)

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RPS7, Monoclonal Antibody (Cat# AAA24641)

Full Name

RPS7 (40S Ribosomal Protein S7) APC

Gene Names

RPS7; S7; DBA8

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between BAD and MAPK8 Mahlavu cells were stained with BAD rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PXN and MAPK8 Huh7 cells were stained with PXN rabbit purified polyclonal 1:1200 and MAPK8 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CRK and MAPK8 HeLa cells were stained with CRK rabbit purified polyclonal 1:1200 and MAPK8 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

WB (Western Blot) (Western blot analysis of MAPK8 over-expressed 293 cell line, cotransfected with MAPK8 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK8 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged MAPK8 is 1ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of MAPK8 expression in transfected 293T cell line by MAPK8 monoclonal antibody. Lane 1: MAPK8 transfected lysate (48.3kD). Lane 2: Non-transfected lysate.)

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MAPK8, Monoclonal Antibody (Cat# AAA25153)

Full Name

MAPK8 (JNK1, PRKM8, SAPK1, SAPK1C, Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, JNK-46, Stress-activated Protein Kinase 1c, Stress-activated Protein Kinase JNK1, c-Jun N-terminal Kinase 1) (FITC)

Gene Names

MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2

Reactivity

Human

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (Western blot analysis of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged GPR3 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GPR3 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody. Lane 1: GPR3 transfected lysate (35kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GPR3 monoclonal antibody, Western Blot analysis of GPR3 expression in Jurkat.)

WB (Western Blot) (Western Blot detection against Immunogen (62.04kD).)

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GPR3, Monoclonal Antibody (Cat# AAA25409)

Full Name

GPR3 (G-protein Coupled Receptor 3, ACCA Orphan Receptor, ACCA) (HRP)

Gene Names

GPR3; ACCA

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody. Lane 1: IRF2 transfected lysate (39.4kD). Lane 2: Non-transfected lysate.)

Application Data (Detection limit for is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence in HeLa cells using (10ug/ml).)

IHC (Immunohistochemistry) (Immunoperoxidase in formalin-fixed paraffin-embedded human leiomyosarcoma usign (3ug/ml).)

WB (Western Blot) (Western Blot analysis of IRF2 expression in Hela NE using.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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IRF2, Monoclonal Antibody (Cat# AAA26689)

Full Name

IRF2 (IRF-2, Interferon Regulatory Factor 2) (MaxLight 490)

Gene Names

IRF2; IRF-2

Reactivity

Human

Applications

FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of NIH/3T3 cells with Smad2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Smad2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Smad2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Smad2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-Smad2 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Smad2 on different lysates using anti-Smad2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: PC12)

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SMAD2, Monoclonal Antibody (Cat# AAA30017)

Full Name

SMAD2 Antibody

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells with Exportin-5 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Exportin-5 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Exportin-5 on 293 cell using anti-Exportin-5 antibody at 1/2, 000 dilution.)

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Exportin-5, Monoclonal Antibody (Cat# AAA30529)

Full Name

Exportin-5 Antibody

Gene Names

XPO5; exp5

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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Application Data (Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. High power)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD8 Alpha: Alexa Fluor 488)

Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: FITC)

Application Data (Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. Medium power)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha: Biotin)

Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE - Alexa Fluor 647)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha:RPE)

Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE)

Application Data (Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. Low power)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8)

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CD8 ALPHA, Monoclonal Antibody (Cat# AAA11842)

Full Name

RAT ANTI MOUSE CD8 ALPHA:APC

Gene Names

Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331

Applications

Flow cytometry (FC/FACS)

Pricing

$585/100 Tests $1,130/2x100 Tests $1,640/3x100 Tests $2,125/4x100 Tests $2,380/5x100 Tests

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IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using beta actin Monoclonal Antibody at dilution of 1:200.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using beta actin Monoclonal Antibody at dilution of 1:200.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using beta actin Monoclonal Antibody at dilution of 1:200.)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using beta actin Monoclonal Antibody at dilution of 1:200.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human Lung cancer using beta actin Monoclonal Antibody at dilution of 1:1000.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human Stomach cancer using beta actin Monoclonal Antibody at dilution of 1:1000.)

WB (Western Blot) (Western blot with Anti beta actin Monoclonal Antibody at dilution of 1:3000. Lane 1: Raji cell lysate, Lane 2: Jurkat cell lysate, Lane 3: THP-1 cell lysate, Lane 4: Mouse spleen tissue lysate, Lane 5: Rat spleen tissue lysate, Lane 6: Raw264.7 cell lysate, Lane 7: C6 cell lysate.)

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beta actin, Monoclonal Antibody (Cat# AAA22338)

Full Name

beta actin Monoclonal Antibody

Gene Names

ACTB; BRWS1; PS1TP5BP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purity

Protein A/G Purification

Pricing

$160/0.025 mL $225/0.1 mL $425/2x0.1 mL $615/3x0.1 mL $795/4x0.1 mL $955/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody (M02), clone 1D11.Lane 1: TRIM28 transfected lysate (Predicted MW: 88.5 KDa).Lane 2: Non-transfected lysate.)

WB (Western Blot) (TRIM28 monoclonal antibody (M02), clone 1D11. Western Blot analysis of TRIM28 expression in MCF-7.)

WB (Western Blot) (TRIM28 monoclonal antibody (M02), clone 1D11. Western Blot analysis of TRIM28 expression in PC-12.)

WB (Western Blot) (TRIM28 monoclonal antibody (M02), clone 1D11. Western Blot analysis of TRIM28 expression in NIH/3T3.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])

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TRIM28, Monoclonal Antibody (Cat# AAA26180)

Full Name

TRIM28 (Tripartite Motif-Containing 28, FLJ29029, KAP1, RNF96, TF1B, TIF1B) (Biotin)

Gene Names

TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IP (Immunoprecipitation) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)

ELISA (Figure 5. ELISA plate was coated with recombinant BCMA-hFc fusion protein (PME100001), followed by pre-blocking with huC11D5.3 antibody (Grey bar) or rabbit control IgG (Black bar), and then different rabbit DimAbs antibodies were added to check the competitive inhibition of huC11D5.3. DM3 clone exhibits the strongest inhibition (Red bar). This data indicated that DM3 bind to the same epitope as bb2121.)

Application Data (Figure 4. Affinity ranking of different DimAb clones by titration of rabbit DimAb antibody concentration onto K562-BCMA or NCI-H929 cells. Different concentrations of various anti-BCMA DimAb clones were incubated with K562-BCMA (A) or NCI-H929 cells (B) at 4 degree C. Bound rabbit IgG was detected in flow cytometry analysis. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Application Data (Figure 3. Phylogenetic analysis of different Anti-BCMA DimAb clones. A) heavy chain and B) Light chain.)

FCM (Flow Cytometry) (Figure 2. A. Flow cytometry analysis with anti-BCMA (DM5) on NCI-H929 cells (Red histogram) or rabbit control antibody on NCI-H929 cells (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM5) on NCI-H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

FCM (Flow Cytometry) (Figure 1. A. Flow cytometry analysis with anti-BCMA (DM5) on K562-BCMA (Red histogram) (K562 cells stably transduced by human BCMA full length gene) and K562 (Negative control cell line) (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM5). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

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BCMA, Monoclonal Antibody (Cat# AAA27972)

Full Name

Anti-BCMA antibody (DM5), Rabbit mAb

Reactivity

Human

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF)

Purity

Purified from cell culture supernatant by affinity chromatography

Pricing

$385/0.01 mL $565/0.1 mL (AF350) $565/0.1 mL (AF405L) $565/0.1 mL (AF405M) $565/0.1 mL (AF405S) $565/0.1 mL (AF488) $565/0.1 mL (AF546) $565/0.1 mL (AF555) $565/0.1 mL (AF568) $565/0.1 mL (AF594) $565/0.1 mL (AF610) $565/0.1 mL (AF635) $565/0.1 mL (AF647) $565/0.1 mL (AF680) $565/0.1 mL (AF750) $565/0.1 mL (Biotin) $565/0.1 mL (FITC) $565/0.1 mL (HRP) $695/0.1 mL (AP) $695/0.1 mL (APC) $695/0.1 mL (Cy3) $695/0.1 mL (Cy5) $695/0.1 mL (Cy5.5) $695/0.1 mL (Cy7) $695/0.1 mL (PE) $1,345/2x0.1 mL (AP) $1,955/3x0.1 mL (AP) $2,535/4x0.1 mL (AP)

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ICC (Immunocytochemistry) (ICC staining MSH6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MSH6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-MSH6 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MSH6 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MSH6 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MSH6 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of MSH6 on different lysates using anti-MSH6 antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: SW480 Lane 3: A549)

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MSH6, Monoclonal Antibody (Cat# AAA30020)

Full Name

MSH6 Antibody

Gene Names

MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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WB (Western Blot) (Detection of human FOXQ1 by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, HEK293T, NCI-H520, MUTZ-3, and HT-29 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation) (Detection of human FOXQ1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NCI-H520 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877 lot 1) used for IP at 6 ul/mg lysate. FOXQ1 was also immunoprecipitated by a second antibody against a different epitope of FOXQ1 (BL-2269A-2C5). For blotting immunoprecipitated FOXQ1, AAA23877 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IHC (Immunohistochemistry) (Detection of human FOXQ1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry) (Detection of mouse FOXQ1 by immunohistochemistry. Sample: FFPE section of mouse bladder. Antibody: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of human FOXQ1 by immunocytochemistry. Sample: FFPE section of NCI-H520 cells. Antibody: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human FOXQ1 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

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FOXQ1, Monoclonal Recombinant Antibody (Cat# AAA23877)

Full Name

Rabbit anti-FOXQ1 Recombinant Monoclonal Antibody [BLR230K]

Gene Names

FOXQ1; HFH1

Reactivity

Human

Applications

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

Purity

Purified

Pricing

$265/0.01 mL $640/0.1 mL $930/5x0.01 mL $1,235/2x0.1 mL $1,800/3x0.1 mL $2,330/4x0.1 mL $2,635/5x0.1 mL

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WB (Western Blot) (Western blot analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged PGM1 is 0.3ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of PGM1 transfected lysate using PGM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM1 rabbit polyclonal antibody)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGM1 on HeLa cell. [antibody concentration 20ug/ml])

WB (Western Blot) (Western Blot analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody. Lane 1: PGM1 transfected lysate (61.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PGM1 monoclonal antibody Western Blot analysis of PGM1 expression in Jurkat.)

WB (Western Blot) (Western Blot detection against Immunogen (87.56kD).)

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PGM1, Monoclonal Antibody (Cat# AAA24901)

Full Name

PGM1 (Phosphoglucomutase-1, Glucose Phosphomutase 1) (Biotin)

Gene Names

PGM1; CDG1T; GSD14

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Proximity Ligation Analysis of protein-protein interactions between TP53 and MDM2 HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-MDM2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

WB (Western Blot) (Western blot analysis of MDM2 over-expressed 293 cell line, cotransfected with MDM2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MDM2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged MDM2 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MDM2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 1.5ug/ml])

WB (Western Blot) (Western Blot analysis of MDM2 expression in transfected 293T cell line by MDM2 monoclonal antibody. Lane 1: MDM2 transfected lysate (55.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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MDM2, Monoclonal Antibody (Cat# AAA25157)

Full Name

MDM2 (E3 Ubiquitin-protein Ligase Mdm2, Double Minute 2 Protein, Hdm2, Oncoprotein Mdm2, p53-binding Protein Mdm2) (FITC)

Gene Names

MDM2; HDMX; hdm2; ACTFS

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK3 and RPS6KA2 HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-RPS6KA2 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

WB (Western Blot) (Western blot analysis of RPS6KA2 over-expressed 293 cell line, cotransfected with RPS6KA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RPS6KA2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS6KA2 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RPS6KA2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of RPS6KA2 expression in transfected 293T cell line by RPS6KA2 monoclonal antibody. Lane 1: RPS6KA2 transfected lysate (83.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (RPS6KA2 monoclonal antibody, Western Blot analysis of RPS6KA2 expression in Hela NE.)

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RSK3, Monoclonal Antibody (Cat# AAA24643)

Full Name

RSK3, CT (Ribosomal S6 Kinase 3, RSK-3, pp90RSK3, 90kD Ribosomal Protein S6 Kinase 2, p90-RSK 2, p90RSK2, MAP Kinase-activated Protein Kinase 1c, MAPK-activated Protein Kinase 1c, MAPKAP Kinase 1c, MAPKAPK1C, MAPKAPK-1c, Ribosomal Protein S6 Kinase alpha-

Gene Names

RPS6KA2; RSK; HU-2; RSK3; p90RSK2; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)

Application Data (Detection limit for recombinant GST tagged MCM2 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of MCM2 transfected lysate using MCM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MCM2 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM2 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody. Lane 1: MCM2 transfected lysate (101.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (MCM2 monoclonal antibody Western Blot analysis of MCM2 expression in HeLa NE.)

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MCM2, Monoclonal Antibody (Cat# AAA25155)

Full Name

MCM2 (DNA Replication Licensing Factor MCM2, Minichromosome Maintenance Protein 2 Homolog, Nuclear Protein BM28, BM28, CCNL1, CDCL1, KIAA0030, MGC10606) (FITC)

Gene Names

MCM2; BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IP (Immunoprecipitation) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)

ELISA (Figure 5. ELISA plate was coated with recombinant BCMA-hFc fusion protein (PME100001), followed by pre-blocking with huC11D5.3 antibody (Grey bar) or rabbit control IgG (Black bar), and then different rabbit DimAbs antibodies were added to check the competitive inhibition of huC11D5.3. DM3 clone exhibits the strongest inhibition (Red bar). This data indicated that DM3 bind to the same epitope as bb2121.)

Application Data (Figure 4. Affinity ranking of different DimAb clones by titration of rabbit DimAb antibody concentration onto K562-BCMA or NCI-H929 cells. Different concentrations of various anti-BCMA DimAb clones were incubated with K562-BCMA (A) or NCI-H929 cells (B) at 4 degree C. Bound rabbit IgG was detected in flow cytometry analysis. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Application Data (Figure 3. Phylogenetic analysis of different Anti-BCMA DimAb clones. A) heavy chain and B) Light chain.)

FCM (Flow Cytometry) (Figure 2. A. Flow cytometry analysis with anti-BCMA (DM4) on NCI-H929 cells (Red histogram) or rabbit control antibody on NCI-H929 cells (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM4) on NCI-H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

FCM (Flow Cytometry) (Figure 1. A. Flow cytometry analysis with anti-BCMA (DM4) on K562-BCMA (Red histogram) (K562 cells stably transduced by human BCMA full length gene) and K562 (Negative control cell line) (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM4). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

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BCMA, Monoclonal Antibody (Cat# AAA27971)

Full Name

Anti-BCMA antibody (DM4), Rabbit mAb

Reactivity

Human

Applications

ELISA (EIA), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF)

Purity

Purified from cell culture supernatant by affinity chromatography

Pricing

$385/0.01 mL $565/0.1 mL (AF350) $565/0.1 mL (AF405L) $565/0.1 mL (AF405M) $565/0.1 mL (AF405S) $565/0.1 mL (AF488) $565/0.1 mL (AF546) $565/0.1 mL (AF555) $565/0.1 mL (AF568) $565/0.1 mL (AF594) $565/0.1 mL (AF610) $565/0.1 mL (AF635) $565/0.1 mL (AF647) $565/0.1 mL (AF680) $565/0.1 mL (AF750) $565/0.1 mL (Biotin) $565/0.1 mL (FITC) $565/0.1 mL (HRP) $695/0.1 mL (AP) $695/0.1 mL (APC) $695/0.1 mL (Cy3) $695/0.1 mL (Cy5) $695/0.1 mL (Cy5.5) $695/0.1 mL (Cy7) $695/0.1 mL (PE) $1,345/2x0.1 mL (AP) $1,955/3x0.1 mL (AP) $2,535/4x0.1 mL (AP)

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Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . Low power)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . High power)

Application Data (Published customer image: Massive activation and productive infection of microglia in CD4-depleted RMs. (a) ISH showing the levels of SIV vRNA+ cells in brain from a representative SIV-uninfected control (top), SIV-infected control (middle) and CD4-depleted SIV-infected (bottom) animal. The amount of SIV vRNA+ cells was markedly higher in CD4-depleted animals than in controls. (b) Immunofluorescence staining for CD163 (left panels), HLA-DR (middle panels) and proliferating cell nuclear antigen (PCNA; right panels) in the parenchyma of one representative SIV-uninfected control (top), one SIV-infected control (middle) and one SIV-infected CD4-depleted (bottom) RM. Nuclei are stained in green; markers of interest in red. (c) Quantitative analyses showing the number of cells per mm2 of tissue that stained positively for the markers of interest. Increased expression of CD163, HLA-DR and PCNA was found in CD4-depleted animals (orange square; n = 4) when compared to both groups of controls (SIV- open circle, n = 3; SIV+ closed circle, n = 3). (d) Single and combined staining for IBA-1 (green), CD163 (blue), and SIV-vRNA (red) in the brain of one representative SIV-infected CD4 depleted RM. The box on the right is a magnification of a microglial cell (IBA-1+) that expresses CD163 and is productively infected (SIV vRNA+).From: Micci L, Alvarez X, Iriele RI, Ortiz AM, Ryan ES, et al. (2014) CD4 Depletion in SIV-Infected Macaques Results in Macrophage and Microglia Infection with Rapid Turnover of Infected Cells. PLoS Pathog 10(10): e1004467.)

Application Data (Published customer image: Massive SIV infection of LN and intestinal macrophages in CD4-depleted RMs. (a) Immunofluorescence staining for T cell (CD3; blue) and macrophage (CD68 and/or CD163; green) lineage markers combined with in situ hybridization for SIV vRNA (red) in the LN isolated at day 42 post-infection from one representative undepleted control (left) and one CD4-depleted RM. The vast majority of SIV vRNA+ cells express CD3 in undepleted control but express CD68/CD163 in CD4-depleted RM. (b) The same staining is shown for colon (top) and jejunum (bottom) tissues in two representative CD4-depleted animals. (c) Quantitative image analysis of LN and mucosal tissues showing the fraction of SIV vRNA+ cells that express CD3 or CD68/CD163 in CD4-depleted (n = 12; the 8 animals in this study plus the 4 in Ortiz et al 2011) or undepleted control RMs (n = 6; two SIVmac251 infected animals belonging to a different study were added as controls). (d) Relative abundance of SIV vRNA+ in productively infected T cells and macrophages, determined by measuring the volumetric sum of the SIV vRNA+ intensity integration values in situ from confocal images collected under identical laser settings, is shown in four CD4-depleted RMs. Macrophages on average have higher per cell SIV vRNA+ content compared to CD4+ T cells within the same host. Statistical analyses were determined by Mann-Whitney test.From: Micci L, Alvarez X, Iriele RI, Ortiz AM, Ryan ES, et al. (2014) CD4 Depletion in SIV-Infected Macaques Results in Macrophage and Microglia Infection with Rapid Turnover of Infected Cells. PLoS Pathog 10(10): e1004467.)

Application Data (Published customer image:Immunofluorescence analysis of myocardial HO-1 and CD163 expression. Surgical non-treated (NT) control animals showed minimal HO-1 (green) expression in resident CD163+ perivascular macrophages (red) (A -C). Sporadic HO-1 expression (white arrow) localized to CD163+ cells observed with Hb alone (D -F). With LPS alone, CD163+ infiltrates and perivascular macrophages showed minimal HO-1 expression (white arrowhead) (G -I). Increased HO-1 expression localized to CD163+ infiltrates and perivascular macrophages observed with LPS + Hb (white arrows) (J -L,M -O). Hp reduces HO-1 expression in CD163+ infiltrates and perivascular cells (P -R,S -U). Nuclei were counterstained with Hoechst 33342 (blue). Scale bar = 10 um.From: Baek JH, Zhang X, Williams MC, Schaer DJ, Buehler PW, D'Agnillo F. Extracellular Hb enhances cardiac toxicity in endotoxemic guinea pigs: protective role of haptoglobin. Toxins (Basel). 2014 Mar 31;6(4):1244-59.)

Application Data (Published customer image: Rasmussen's encephalitis. (A) Areas of active encephalitis and cortical scarring highlighted with increased numbers of HLADR-positive microglia/macrophages, as well as interstitial rod-like CD163-positive microglia cells (inset). (B) On adjacent sections, labelling with pS6 235/236 showed a similar distribution of cellular labelling as well as with pS6 240/244 (C). (D) pS6 240/244 highlighted many of the enlarged dysmorphic neurones in RE cases, as confirmed with co-labelling with neurofilaments (inset). (E) pS6 236/26 demonstrated labelling of smaller glial cells as well as (F) bipolar rod shape cells. Double labelling studies in RE cases confirmed the majority of pS6 labelled cells were not GFAP-positive astroglia (G) but co-labelled with populations of iba1-positive cells (microglial marker) (H), nestin and doublecortin (inset) (I) positive cells. Bar in A, B, C equivalent to approximately 100 microns, in d approximately 50 microns and E, F, G, H, I approximately 30 microns.From: Liu J, Reeves C, Michalak Z, Coppola A, Diehl B, Sisodiya SM, Thom M. Evidence for mTOR pathway activation in a spectrum of epilepsy-associated pathologies. Acta Neuropathol Commun. 2014 Jul 8;2:71.)

Application Data (Published customer image: Tumor-promoting phenotype of the myeloid clusters. IHC staining demonstrating the expression of CD163, IL-6, IL-10, VEGF-A, MMP-9, SDF-1 and Bcl-xL by the myeloid cells associated with anthracosis. For each protein, representative images showing positive and negative staining areas were selected from the same slide. The quantification was performed by analyzing random images of myeloid cluster areas associated with anthracosis and other areas (10 images for each group) from 10 patients. Two-tailed Student's t-test was used for statistical analysis. Shown are means +/- SEM, *** P)

Application Data (Published customer image: Morphologic and phenotypic characterization of porcine alveolar macrophage cells (PAM) by flow cytometry using SWC3, SWC1, CD163, CD14, CD16, MHCII and DC-sign staining. This figure is a representative of three independent experiments.From: Seeboth J, Solinhac R, Oswald IP, Guzylack-Piriou L. The fungal T-2 toxin alters the activation of primary macrophages induced by TLR-agonists resulting in a decrease of the inflammatory response in the pig. Vet Res. 2012 Apr 24;43:35..)

Application Data (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

Application Data (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

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CD163, Monoclonal Antibody (Cat# AAA12100)

Full Name

MOUSE ANTI HUMAN CD163

Gene Names

CD163; M130; MM130

Applications

Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoassay (IY), Immunohistology Paraffin, Western Blot (WB)

Pricing

$260/0.025 mg $495/2x0.025 mg $715/3x0.025 mg $920/5x0.025 mg $925/4x0.025 mg

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FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells with VEGF Receptor 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining VEGF Receptor 1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining VEGF Receptor 1 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining VEGF Receptor 1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VEGF Receptor 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-VEGF Receptor 1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of VEGF Receptor 1 on mouse lung lysates using anti-VEGF Receptor 1 antibody at 1/1, 000 dilution.)

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VEGF 1, Monoclonal Antibody (Cat# AAA30023)

Full Name

VEGF Receptor 1 Antibody

Gene Names

FLT1; FLT; FLT-1; VEGFR1; VEGFR-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CTNNBL1 mouse mAb with DAB staining.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CTNNBL1 mouse mAb with DAB staining.)

FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells using CTNNBL1 mouse mAb (green) and negative control (red).)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using CTNNBL1 mouse mAb (green). Blue)

WB (Western Blot) (Western blot analysis using CTNNBL1 mouse mAb against Hela (1), Jurkat (2), HEK293 (3), A431 (4), HepG2 (5), RAJI (6) cell lysate.)

WB (Western Blot) (Western blot analysis using CTNNBL1 mAb against HEK293 (1) and CTNNBL1 (AA)

WB (Western Blot) (Western blot analysis using CTNNBL1 mAb against human CTNNBL1 (AA)

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CTNNBL1, Monoclonal Antibody (Cat# AAA14152)

Full Name

Anti-CTNNBL1 Mouse mAb

Gene Names

CTNNBL1; NAP; P14L; PP8304; C20orf33; dJ633O20.1

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)

Pricing

$525/0.1 mL $1,010/2x0.1 mL $1,470/3x0.1 mL $1,810/5x0.1 mL $1,905/4x0.1 mL

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WB (Western Blot) (TRIB2 monoclonal antibody Western Blot analysis of TRIB2 expression in Jurkat.)

WB (Western Blot) (Western blot analysis of TRIB2 over-expressed 293 cell line, cotransfected with TRIB2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIB2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged TRIB2 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TRIB2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of TRIB2 expression in transfected 293T cell line by TRIB2 monoclonal antibody. Lane 1: TRIB2 transfected lysate (38.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)

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TRIB2, Monoclonal Antibody (Cat# AAA24392)

Full Name

TRIB2 (Tribbles Homolog 2, TRB2, TRB-2, C5FW, GS3955) (AP)

Gene Names

TRIB2; C5FW; TRB2; GS3955

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of DHFR expression in HeLa.)

IF (Immunofluorescence) (Immunofluorescence on HeLa cells using 125814 (10ug/ml).)

Application Data (Detection limit for 125814 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of DHFR expression in transfected 293T cell line using 125814. Lane 1: DHFR transfected lysate (21.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot analysis of DHFR expression in PC-12 using 125814.)

WB (Western Blot) (Western Blot detection against immunogen (36.74kD).)

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DHFR, Monoclonal Antibody (Cat# AAA26696)

Full Name

DHFR (Dihydrofolate reductase) (MaxLight 650)

Gene Names

DHFR; DYR; DHFRP1

Reactivity

Human, Rat

Applications

FLISA, Immunofluorescence (IF), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with PHD1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining PHD1 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PHD1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PHD1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of PHD1 on different lysates using anti-PHD1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: PC12 Lane 3: NIH/3T3)

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PHD1/Prolyl Hydroxylase, Monoclonal Antibody (Cat# AAA30022)

Full Name

PHD1/Prolyl Hydroxylase Antibody

Gene Names

EGLN2; EIT6; PHD1; HPH-1; HPH-3; HIFPH1; HIF-PH1

Reactivity

Human, Mouse, Rat

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of 293T cells with ABCG2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

ICC (Immunocytochemistry) (ICC staining ABCG2 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining ABCG2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining ABCG2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-ABCG2 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of ABCG2 on different lysates using anti-ABCG2 antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: Human placenta)

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ABCG2, Monoclonal Antibody (Cat# AAA30278)

Full Name

ABCG2 Antibody

Gene Names

ABCG2; MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; CDw338; UAQTL1; EST157481

Reactivity

Human, Mouse

Applications

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Purity

ProA affinity purified

Pricing

$360/0.05 mL $465/0.1 mL $895/2x0.1 mL $1,300/3x0.1 mL $1,680/4x0.1 mL $1,875/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CAV2 mouse mAb with DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using CAV2 mouse mAb with DAB staining.)

FCM (Flow Cytometry) (Flow cytometric analysis of A549 cells using CAV2 mouse mAb (green) and negative control (red).)

WB (Western Blot) (Western blot analysis using CAV2 mouse mAb against A549 (1), 3T3-L1 (2), A431 (3) cell lysate.)

WB (Western Blot) (Western blot analysis using CAV2 mAb against HEK293 (1) and CAV2 (AA)

WB (Western Blot) (Western blot analysis using CAV2 mAb against human CAV2 (AA)

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CAV2, Monoclonal Antibody (Cat# AAA14151)

Full Name

Anti-CAV2 Mouse mAb

Gene Names

CAV2; CAV

Reactivity

Human

Applications

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Pricing

$525/0.1 mL $1,010/2x0.1 mL $1,470/3x0.1 mL $1,810/5x0.1 mL $1,905/4x0.1 mL

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WB (Western Blot) (Western blot analysis of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DUSP6 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged DUSP6 is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DUSP6 on HeLa cell. [antibody concentration 10ug/ml.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DUSP6 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of DUSP6 expression in transfected 293T cell line by DUSP6 monoclonal antibody. Lane 1: DUSP6 transfected lysate (42kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (67.65kD).)

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DUSP6, Monoclonal Antibody (Cat# AAA25671)

Full Name

DUSP6 (Dual Specificity Protein Phosphatase 6, Dual Specificity Protein Phosphatase PYST1, Mitogen-activated Protein Kinase Phosphatase 3, MAP Kinase Phosphatase 3, MKP3, MKP-3, PYST1) (PE)

Gene Names

DUSP6; HH19; MKP3; PYST1

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence to CDKN1B on HeLa cell using 124808 (10ug/ml).)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between AKT1 and CDKN1B HeLa cells were stained with AKT1 rabbit purified polyclonal (1:1200) and 124808 (1:50). Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

IHC (Immunohistochemistry) (Immunoperoxidase to CDKN1B on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue using 124808 (5ug/ml).)

WB (Western Blot) (Western Blot analysis of CDKN1B expression in transfected 293T cell line by 124808 Lane 1: CDKN1B transfected lysate (22.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (47.89kD).)

Application Data (Detection limit for 124808 is 10ng/ml as a capture antibody.)

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CDKN1B, Monoclonal Antibody (Cat# AAA26695)

Full Name

CDKN1B (Cyclin-dependent Kinase Inhibitor 1B, Cyclin-dependent Kinase Inhibitor p27, p27Kip1, KIP1) (MaxLight 650)

Gene Names

CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1

Reactivity

Human

Applications

FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

WB (Western Blot) (ASNA1 monoclonal antibody. Western Blot analysis of ASNA1 expression in PC-12.)

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ASNA1, Monoclonal Antibody (Cat# AAA24143)

Full Name

ASNA1 (ATPase ASNA1, Arsenical Pump-driving ATPase, Arsenite-stimulated ATPase, Transmembrane Domain Recognition Complex 40kD ATPase Subunit, hARSA-I, hASNA-I, ARSA, TRC40) (AP)

Gene Names

ASNA1; GET3; ARSA1; TRC40; ARSA-I; ASNA-I

Reactivity

Human, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged UBE2C is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBE2C on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBE2C on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of UBE2C expression in transfected 293T cell line by UBE2C monoclonal antibody. Lane 1: UBE2C transfected lysate (19.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (UBE2C monoclonal antibody, Western Blot analysis of UBE2C expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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UBE2C, Monoclonal Antibody (Cat# AAA24399)

Full Name

UBE2C (Ubiquitin-conjugating Enzyme E2 C, UbcH10, Ubiquitin Carrier Protein C, Ubiquitin-protein Ligase C, dJ447F3.2) (AP)

Gene Names

UBE2C; UBCH10; dJ447F3.2

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged SHMT1 is ~1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 1ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of SHMT1 expression in transfected 293T cell line by SHMT1 monoclonal antibody. Lane 1: SHMT1 transfected lysate (53.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SHMT1 monoclonal antibody, Western Blot analysis of SHMT1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)

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SHMT1, Monoclonal Antibody (Cat# AAA24655)

Full Name

SHMT1 (Serine Hydroxymethyltransferase, Cytosolic, SHMT, Serine Methylase, Glycine Hydroxymethyltransferase, MGC15229, MGC24556) APC

Gene Names

SHMT1; SHMT; CSHMT

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (Western blot analysis of MRPL12 over-expressed 293 cell line, cotransfected with MRPL12 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MRPL12 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged MRPL12 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MRPL12 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MRPL12 on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody. Lane 1: MRPL12 transfected lysate (21.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (MRPL12 monoclonal antibody, Western Blot analysis of MRPL12 expression in COLO 320 HSR.)

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MRPL12, Monoclonal Antibody (Cat# AAA25167)

Full Name

MRPL12 (Mitochondrial Ribosomal Protein L12, 39S Ribosomal Protein L12, Mitochondrial, 5c5-2, FLJ60124, L12mt, MGC8610, MRP-L12, MRP-L31/34, MRPL7, MRPL7/L12, RPML12) (FITC)

Gene Names

MRPL12; 5c5-2; L12mt; MRPL7; RPML12; MRPL7/L12; MRP-L31/34

Reactivity

Human

Applications

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in Raw 264.7.)

WB (Western Blot) (HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in NIH/3T3)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between TP53 and HSPA1B. HeLa cells were stained with TP53 rabbit purified polyclonal 1:1200 and HSPA1B mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged HSPA1B is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HSPA1B on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human testis. [antibody concentration 6ug/ml])

WB (Western Blot) (Western Blot detection against Immunogen (34.65kD).)

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HSPA1A, Monoclonal Antibody (Cat# AAA25423)

Full Name

HSPA1A (Heat Shock 70kD Protein 1A/1B, Heat Shock 70kD Protein 1/2, HSP70.1/HSP70.2, HSP70-1/HSP70-2, HSPA1, HSPA1B) (HRP)

Gene Names

HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PGR is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (PGR monoclonal antibody (M04), clone 2C2 Western Blot analysis of PGR expression in A-431.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGR on A-431 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGR on A-431 cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGR on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1.5 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to PGR on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1.5 ug/ml])

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PGR, Monoclonal Antibody (Cat# AAA26191)

Full Name

PGR (Progesterone Receptor, NR3C3, PR) (Biotin)

Gene Names

PGR; PR; NR3C3

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (Detection limit for recombinant GST tagged TESK2 is approximately 0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])

WB (Western Blot) (Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M04), clone 5H4.Lane 1: TESK2 transfected lysate (60.3 KDa).Lane 2: Non-transfected lysate.)

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TESK2, Monoclonal Antibody (Cat# AAA26447)

Full Name

TESK2 (Testis-Specific Kinase 2) (HRP)

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)

Purity

Purified

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts of various cells, using DYNLT1 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using DYNLT1 antibody.)

Application Data

WB (Western Blot) (Western blot analysis of extracts of various cells, using DYNLT1 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using DYNLT1 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using DYNLT1 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using DYNLT1 antibody.)

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Atrial Natriuretic Peptide, Monoclonal Antibody (Cat# AAA26703)

Full Name

Atrial Natriuretic Peptide (ANP, Atrial Natriuretic Factor) (APC)

Gene Names

NPPA; ANF; ANP; PND; ATFB6; CDD-ANF

Reactivity

Human

Applications

ELISA (EIA), Immunohistochemistry (IHC), Radioimmunoassay (RIA)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,350/0.1 mL $2,620/2x0.1 mL $3,820/3x0.1 mL $4,955/4x0.1 mL $5,855/5x0.1 mL

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284: Low Endotoxin)

Application Data (Staining of U937 cells with Mouse anti Human CD284:RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284: FITC)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284: Alexa Fluor 647)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD284: Alexa Fluor 488)

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CD284, Monoclonal Antibody (Cat# AAA12112)

Full Name

MOUSE ANTI HUMAN CD284:FITC

Gene Names

TLR4; TOLL; CD284; TLR-4; ARMD10

Applications

Flow cytometry (FC/FACS)

Pricing

$250/0.025 mg $475/2x0.025 mg $685/3x0.025 mg $870/5x0.025 mg $885/4x0.025 mg

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WB (Western Blot) (Western Blot Analysis of Human Spleen cell lysate using HLA-Pan Mouse Monoclonal Antibody (CR3/43).)

WB (Western Blot) (Western Blot Analysis of Ramos cell lysate using HLA-Pan Mouse Monoclonal Antibody (CR3/43).)

SDS-PAGE (SDS-PAGE Analysis Purified HLA-Pan Mouse Monoclonal Antibody (CR3/43). Confirmation of Purity and Integrity of Antibody.)

IF (Immunofluorescence) (Immunofluorescent staining of Raji cells. HLA- Pan Mouse Monoclonal Antibody (CR3/43) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red))

FCM (Flow Cytometry) (Flow Cytometric Analysis of Human Raji cells using HLA-Pan Mouse Monoclonal Antibody (CR3/43) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Tonsil stained with HLA- Pan Mouse Monoclonal Antibody (CR3/43).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Tonsil stained with HLA- Pan Mouse Monoclonal Antibody (CR3/43).)

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HLA-DP/-DQ/-DR, Monoclonal Antibody (Cat# AAA23889)

Full Name

HLA-DP/-DQ/-DR (MHC II)

Gene Names

HLA-DPB1; DPB1; HLA-DP; HLA-DPB; HLA-DP1B

Reactivity

Human

Applications

Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

Pricing

$265/0.02 mg (With BSA & Azide at 0.2mg/mL) $445/0.1 mg (With BSA & Azide at 0.2mg/mL) $445/0.1 mg (Without BSA & Azide at 1mg/mL) $855/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,245/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,610/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,810/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,810/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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WB (Western Blot) (Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged MUTYH is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody. Lane 1: MUTYH transfected lysate (59.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (MUTYH monoclonal antibody Western Blot analysis of MUTYH expression in HeLa NE.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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MUTYH, Monoclonal Antibody (Cat# AAA25169)

Full Name

MUTYH (A/G-specific Adenine DNA Glycosylase, MutY Homolog, hMYH, MYH) (FITC)

Gene Names

MUTYH; MYH

Reactivity

Human

Applications

ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

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WB (Western Blot) (EXOC4 monoclonal antibody. Western Blot analysis of EXOC4 expression in Raw 264.7.)

Application Data (Detection limit for recombinant GST tagged EXOC4 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (EXOC4 monoclonal antibody. Western Blot analysis of EXOC4 expression in NIH/3T3.)

WB (Western Blot) (EXOC4 monoclonal antibody, Western Blot analysis of EXOC4 expression in Hela NE.)

WB (Western Blot) (EXOC4 monoclonal antibody. Western Blot analysis of EXOC4 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (38.1kD).)

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EXOC4, Monoclonal Antibody (Cat# AAA25681)

Full Name

EXOC4 (Exocyst Complex Component 4, Exocyst Complex Component Sec8, KIAA1699, SEC8, SEC8L1, MGC27170) (PE)

Gene Names

EXOC4; SEC8; Sec8p; SEC8L1

Reactivity

Human, Mouse, Rat

Applications

ELISA (EIA), Western Blot (WB)

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$990/0.1 mL $1,920/2x0.1 mL $2,795/3x0.1 mL $3,625/4x0.1 mL $4,230/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.