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WB (Western Blot) (SMAD1 monoclonal antibody (M03), clone 2E9. Western Blot analysis of SMAD1 expression in IMR-32.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (SMAD1 monoclonal antibody (M03), clone 2E9 Western Blot analysis of SMAD1 expression in HeLa.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])
ICC (Immunocytochemistry) (ICC staining Phospho-RSK1 p90 (T359+S363) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-RSK1 p90 (T359+S363) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-RSK1 p90 (T359+S363) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Phospho-RSK1 p90 (T359+S363) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-RSK1 p90 (T359+S363) antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Phospho-RSK1 p90 (T359+S363) on different lysates using anti-Phospho-RSK1 p90 (T359+S363) antibody at 1/1, 000 dilution. Positive control: Lane 1: A431 Lane 2: 293 Lane 3: MCF-7)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. Medium power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD21:FITC)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD21:RPE)
Application Data (Immunofluorescence staining of human tonsil cryosection using Mouse anti Human CD1a antibody , red in A and Mouse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. Medium power)
Application Data (Immunofluorescence image of human tonsil cryosection stained with Sheep anti Human LOX-1 antibody in red and with Mouse anti Human CD21 antibody in red. Nuclei are stained blue using DAPI. Merged image on right)
Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 , red in A and Mouse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. medium power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD21)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. High power)
Application Data (Immunoperoxidase staining of human spleen cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. High power)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . Medium power)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. Medium power)
Application Data (Staining of mouse peripheral blood mononuclear cells with Rat anti Mouse CD13: Alexa Fluor 488)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. Low power)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . Low power)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . High power)
Application Data (Staining of mouse peripheral blood mononuclear cells with Rat anti Mouse CD13: Alexa Fluor 647)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. High power)
WB (Western Blot) (Western blot analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARID3A monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged ARID3A is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ARID3A on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ARID3A on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
WB (Western Blot) (ARID3A monoclonal antibody, Western Blot analysis of ARID3A expression in K-562.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
ARID3A (AT-rich Interactive Domain-containing Protein 3A, ARID Domain-containing Protein 3A, B Cell Regulator of IgH Transcription, Bright, Dead Ringer-like Protein 1, E2F-binding Protein 1, DRIL1, DRIL3, DRX, E2FBP1) (AP)
Gene Names
ARID3A; DRIL1; DRIL3; BRIGHT; E2FBP1
Reactivity
Human
Applications
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Data (Proximity Ligation Analysis of protein-protein interactions between CRKL and PIK3R1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between FLT1 and PIK3R1 Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between EGFR and PIK3R1. HeLa cells were stained with EGFR rabbit purified polyclonal 1:1200 and PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue))
Application Data (Detection limit for recombinant GST tagged PIK3R1 is ~0.1ng/ml as a capture antibody.)
WB (Western Blot) (PIK3R1 monoclonal antibody Western Blot analysis of PIK3R1 expression in PC-12.)
WB (Western Blot) (PIK3R1 monoclonal antibody Western Blot analysis of PIK3R1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (75.68kD).)
IP (Immunoprecipitation) (Immunoprecipitation analysis of 600 ug extracts of Mouse brain using 3 ug Src antibody (AAA28491). Western blot was performed from the immunoprecipitate using Src antibody (AAA28491) at a dilution of 1:1000.)
ICC (Immunocytochemistry) (Confocal imaging of NIH/3T3 cells using [KO Validated] Src Rabbit mAb (AAA28491,at dilution of 1:100) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.)
ICC (Immunocytochemistry) (Confocal imaging of A549 cells using [KO Validated] Src Rabbit mAb (AAA28491,at dilution of 1:100) (Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.)
WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using [KO Validated] Src Rabbit mAb (AAA28491) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1min.)
WB (Western Blot) (Western blot analysis of lysates from wild type (WT) and Src knockout (KO) HeLa cells, using [KO Validated] Src Rabbit mAb (AAA28491) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1min.)
WB (Western Blot) (Western blot analysis of various lysates using [KO Validated] Src Rabbit mAb (AAA28491) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot) (Western Blot analysis of TUBA1 expression in transfected 293T cell line by TUBA1 monoclonal antibody. Lane 1: TUBA1 transfected lysate (49.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TUBA1 monoclonal antibody. Western Blot analysis of TUBA1 expression in HeLa.)
WB (Western Blot) (Western blot analysis of TUBA1 over-expressed 293 cell line, cotransfected with TUBA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TUBA1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TUBA1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TUBA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])
WB (Western Blot) (TUBA1 monoclonal antibody. Western Blot analysis of TUBA1 expression in different Cell lines and Human Tissue.)
WB (Western Blot) (Western Blot detection against Immunogen (75.02kD).)
WB (Western Blot) (Western Blot analysis of ATP2C1 expression in transfected 293T cell line by ATP2C1 monoclonal antibody. Lane 1: ATP2C1 transfected lysate (100.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (42.61kD).)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using SUPT5H/SPT5 Rabbit mAb (AAA28496) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using SUPT5H/SPT5 Rabbit mAb (AAA28496) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using SUPT5H/SPT5 Rabbit mAb (AAA28496) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using SUPT5H/SPT5 Rabbit mAb (AAA28496) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat ovary using SUPT5H/SPT5 Rabbit mAb (AAA28496) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of lysates from HeLa cells, using SUPT5H/SPT5 Rabbit mAb (AAA28496) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Application Data (Staining of KG1 lymphocytes with Mouse anti Human CD59:FITC)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59: Azide Free)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59:RPE)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59:Alexa Fluor488)
Application Data (Published customer image: The effect of protease treatment on influenza virion associated host proteins. Purified influenza A/WSN/33 virus was either mock treated or subjected to overnight digestion with subtilisin followed by concentration through a sucrose cushion. 10 ug of mock infected cell lysate (lane 1) or influenza infected cell lysate (lane 2) and 2 ug of untreated influenza virions (lane 3) or protease treated influenza virions (lane 4) were then analyzed by western blot with antibodies against the indicated proteins. Numbers to the right are molecular weight markers.From: Shaw ML, Stone KL, Colangelo CM, Gulcicek EE, Palese P (2008) Cellular Proteins in Influenza Virus Particles. PLoS Pathog 4(6): e1000085.)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Alexa Fluor 647)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Biotin)
WB (Western Blot) (SMAD4 monoclonal antibody. Western Blot analysis of SMAD4 expression in IMR-32.)
WB (Western Blot) (Western Blot detection against Immunogen (86.46kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CDKN1A and SMAD4 HeLa cells were stained with CDKN1A rabbit purified polyclonal 1:1200 and SMAD4 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged SMAD4 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD4 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (SMAD4 monoclonal antibody Western Blot analysis of SMAD4 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (78.03kD).)
WB (Western Blot) (POLK monoclonal antibody, Western Blot analysis of POLK expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged POLK is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POLK on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody. Lane 1: POLK transfected lysate (54.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western blot analysis of NDN over-expressed 293 cell line, cotransfected with NDN Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NDN monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged NDN is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NDN on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NDN on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5ug/ml])
WB (Western Blot) (Western Blot analysis of NDN expression in transfected 293T cell line by NDN monoclonal antibody. Lane 1: NDN transfected lysate (36.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NDN monoclonal antibody Western Blot analysis of NDN expression in HL-60.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Pacific Blue)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Alexa Fluor 488)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Low power)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Medium power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:RPE)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. High power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:Biotin)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:FITC)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:Alexa Fluor 405)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD4)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R1 and SHC1 Huh7 cells were stained with PIK3R1 rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R1 and SHC1 HeLa cells were stained with PIK3R1 rabbit purified polyclonal 1:1200 and SHC1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged SHC1 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SHC1 on A-431 cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SHC1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1ug/ml].)
WB (Western Blot) (SHC1 monoclonal antibody, Western Blot analysis of SHC1 expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (38.1kD).)
SHC (SHC-transforming Protein 1, SH2 Domain Protein C1, SHC1, SHC-transforming Protein 3, SHC-transforming Protein A, SHCA, Src Homology 2 Domain-containing-transforming Protein C1, FLJ26504) APC
Gene Names
SHC1; SHC; SHCA
Reactivity
Human
Applications
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with p-HSF1 (S326) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary)
ICC (Immunocytochemistry) (ICC staining p-HSF1 (S326) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining p-HSF1 (S326) in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining p-HSF1 (S326) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-p-HSF1 (S326) antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of p-HSF1 (S326) on different lysates using anti-p-HSF1 (S326) antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: BT20 Lane 3: AGS)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Albumin antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Albumin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Albumin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Albumin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Albumin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Albumin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Albumin on Hela cells lysates using anti-Albumin antibody at 1/1, 000 dilution.)
WB (Western Blot) (EIF4G1 monoclonal antibody. Western Blot analysis of EIF4G1 expression in NIH/3T3.)
WB (Western Blot) (EIF4G1 monoclonal antibody, Western Blot analysis of EIF4G1 expression in HepG2.)
Application Data (Detection limit for recombinant GST tagged EIF4G1 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EIF4G1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of EIF4G1 expression in transfected 293T cell line by EIF4G1 monoclonal antibody. Lane 1: EIF4G1 transfected lysate (70.95kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (EIF4G1 monoclonal antibody. Western Blot analysis of EIF4G1 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (FOXA1 monoclonal antibody (M05), clone 3C1. Western Blot analysis of FOXA1 expression in HepG2.)
WB (Western Blot) (FOXA1 monoclonal antibody (M05), clone 3C1 Western Blot analysis of FOXA1 expression in MCF-7.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.5 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.5 ug/ml])
ICC (Immunocytochemistry) (ICC staining Phospho-GSK3 beta (Ser 9) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-GSK3 beta (Ser 9) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-GSK3 beta (Ser 9) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-GSK3 beta (Ser 9) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-GSK3 beta (Ser 9) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Phospho-GSK3 beta (Ser 9) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-GSK3 beta (Ser 9) antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Phospho-GSK3 beta (Ser 9) on different lysates using anti-Phospho-GSK3 beta (Ser 9) antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7)
Application Data (Detection limit for recombinant GST tagged IFI16 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to IFI16 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to IFI16 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of IFI16 expression in transfected 293T cell line by IFI16 monoclonal antibody. Lane 1: IFI16 transfected lysate (82kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (IFI16 monoclonal antibody Western Blot analysis of IFI16 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (EXOSC3 monoclonal antibody, Western Blot analysis of EXOSC3 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged EXOSC3 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of EXOSC3 transfected lysate using EXOSC3 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with EXOSC3 monoclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EXOSC3 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of EXOSC3 expression in transfected 293T cell line by EXOSC3 monoclonal antibody. Lane 1: EXOSC3 transfected lysate (29.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (56.36kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of NIH/3T3 cells with Phospho- PAK1 (S144)+PAK2 (S141)+PAK3 (S139) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Phospho- PAK1 (S144)+PAK2 (S141)+PAK3 (S139) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho- PAK1 (S144)+PAK2 (S141)+PAK3 (S139) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- Phospho- PAK1 (S144)+PAK2 (S141)+PAK3 (S139) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- Phospho- PAK1 (S144)+PAK2 (S141)+PAK3 (S139) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Phospho- PAK1 (S144)+PAK2 (S141)+PAK3 (S139) antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Phospho- PAK1 (S144)+PAK2 (S141)+PAK3 (S139) on different lysates using anti-Phospho- PAK1 (S144)+PAK2 (S141)+PAK3 (S139) antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: SH-SY-5Y)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . Medium power)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. Medium power)
Application Data (Staining of mouse peripheral blood mononuclear cells with Rat anti Mouse CD13: Alexa Fluor 488)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. Low power)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . Low power)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . High power)
Application Data (Staining of mouse peripheral blood mononuclear cells with Rat anti Mouse CD13: Alexa Fluor 647)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. High power)
ICC (Immunocytochemistry) (ICC staining TACC3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining TACC3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TACC3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TACC3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TACC3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of TACC3 on different lysates using anti-TACC3 antibody at 1/1, 000 dilution. Positive control: Lane 1: HT29 Lane 2: Hela)
Application Data (Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EIF2AK2 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EIF2AK2 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EIF2AK2 on HeLa cell. [antibody concentration 30 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EIF2AK2 on HeLa cell. [antibody concentration 30 ug/ml])
WB (Western Blot) (EIF2AK2 monoclonal antibody (M02), clone 1D11 Western Blot analysis of EIF2AK2 expression in K-562.)
Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (YY1 monoclonal antibody (M03), clone 4D2 Western Blot analysis of YY1 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged TYK2 is approximately 1ng/ml as a capture antibody.)
WB (Western Blot) (TYK2 monoclonal antibody (M03), clone 6H1 Western Blot analysis of TYK2 expression in Hela S3 NE.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TYK2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TYK2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TYK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to TYK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using AIF Rabbit mAb (AAA28504) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
ICC (Immunocytochemistry) (ICC staining Phospho-JunD (S255) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-JunD (S255) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-JunD (S255) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Phospho-JunD (S255) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-JunD (S255) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Phospho-JunD (S255) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-JunD (S255) antibody. Counter stained with hematoxylin.)
ICC (Immunocytochemistry) (ICC staining NGF in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NGF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NGF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using anti-NGF antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NGF antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-NGF antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of NGF on Hela cell lysates using anti-NGF antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining CD19 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD19 in PC-3M cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CD19 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD19 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD19 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CD19 on Raji cells lysates using anti-CD19 antibody at 1/1, 000 dilution.)
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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